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WO2014094412A1 - Foliar fertilizer composition containing sodium alginate oligosaccharide and use thereof - Google Patents

Foliar fertilizer composition containing sodium alginate oligosaccharide and use thereof Download PDF

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Publication number
WO2014094412A1
WO2014094412A1 PCT/CN2013/076894 CN2013076894W WO2014094412A1 WO 2014094412 A1 WO2014094412 A1 WO 2014094412A1 CN 2013076894 W CN2013076894 W CN 2013076894W WO 2014094412 A1 WO2014094412 A1 WO 2014094412A1
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WIPO (PCT)
Prior art keywords
acid
foliar fertilizer
sodium alginate
fertilizer composition
alginate oligosaccharide
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PCT/CN2013/076894
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French (fr)
Chinese (zh)
Inventor
赵小明
张运红
杜昱光
尹恒
王文霞
刘启顺
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Dalian Institute of Chemical Physics of CAS
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Dalian Institute of Chemical Physics of CAS
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Publication of WO2014094412A1 publication Critical patent/WO2014094412A1/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/10Fertilisers containing plant vitamins or hormones
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/27Dispersions, e.g. suspensions or emulsions

Definitions

  • Foliar fertilizer composition containing sodium alginate oligosaccharide and application thereof
  • the present invention relates to physiological metabolism of plants, and more particularly to the use of amino acids, sodium alginate oligosaccharides and trace elements as main active substances in plant growth promotion. Background technique
  • oligosaccharides such as chitooligosaccharides have been used in the field of plants since the 1970s, and have functions such as inducing plant resistance and promoting plant growth. Such oligosaccharides are specifically named as oligosaccharides.
  • Alginate oligosaccharide is one of the important molecules of oligosaccharide, which is obtained by enzymatic hydrolysis of sodium alginate from aL-mannuronic acid (M) and bD-guluronic acid (G).
  • M L-mannuronic acid
  • G bD-guluronic acid
  • An oligosaccharide which is linked by a 1,4-glycosidic bond and composed of different GGGMMM fragments has the advantages of relatively low molecular weight, low toxicity, good water solubility, and strong absorption.
  • Amino acid trace element fertilizer containing sodium alginate oligosaccharide as active ingredient has not been reported in the literature and patents. Summary of
  • An amino acid trace element foliar fertilizer wherein the main active ingredients of the foliar fertilizer are amino acids, trace elements and sodium alginate oligosaccharides, and the remaining components are solvents, adjuvants or carriers.
  • the foliar fertilizer composition can be used for cash crops, food crops, vegetables, fruit trees, economic forests, flowers and lawns.
  • the flowering and fruit-preserving rate of flowering plants reached 50-99%, increasing the synthesis of various enzymes and secondary metabolites related to resistance under drought conditions of plants 70%-90%.
  • the active ingredient comprises glycine, alanine, isoleucine, cystine, cysteine, methionine, threonine, serine, phenylalanine, tyrosine, Several of tryptophan, proline, hydroxyproline, glutamic acid, aspartic acid, etc.;
  • the trace element fertilizer is a fertilizer capable of providing plant trace elements, such as boron fertilizer, molybdenum fertilizer, manganese fertilizer , copper fertilizer, iron fertilizer, zinc fertilizer, etc.
  • the sodium alginate oligosaccharide is a natural polysaccharide carbohydrate extracted from kelp, by aL-mannuronic acid (M) and bD-guluronic acid ( Guluronic acid, GM is a copolymer consisting of 1, 4-glycosidically linked and composed of different GGGMMM fragments, with a molecular formula of (C 6 H 7 0 6 Na)n, a
  • the composition is diluted 500 times, sprayed on the foliage of the flower bud stage, sprayed once every 7-10 days, and sprayed 3-4 times continuously.
  • the active component is added to a solvent, a dispersing agent and an auxiliary agent to form a water agent, an emulsifiable concentrate, a soluble liquid agent, a water emulsion, a microemulsion, and a suspending agent.
  • the solvent includes, but is not limited to: water, ethanol, acetone, xylene, toluene, chlorobenzene, cyclohexane, methanol, dimethylformamide, pyrrolidone, cyclohexanone, butanol, pentanol, ethers, One or a mixture of esters, dimethyl sulfoxide, lauryl alcohol, dichloromethane, petroleum distillate, a solvent mass ratio of 10-99%; the auxiliary agent has a nonionic type and an ionic type, including but Not limited to: glacial acetic acid, hydrochloric acid, sulfuric acid, sodium hydroxide, potassium hydroxide, ammonia, fatty alcohol polyoxyethylene ether, alkylphenol ethoxylate, polyoxyethylene fatty acid ester, alkyl aryl polyglycol One of an ether, an alkyl sulfonate, an aryl sulfonate, a fatty acid polydiethanol
  • the mass ratio of additives is 10-99%.
  • the dispersing agent includes one or a mixture of the above-mentioned lignosulfonate, methylcellulose, alkylsulfonate, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether, etc., and the mass ratio is 0.05-50%.
  • the foliar fertilizer composition may be added with zinc sulfate, zinc nitrate, zinc chloride, zinc phosphate, copper sulfate, copper nitrate, copper chloride, copper phosphate, manganese nitrate, manganese sulfate, manganese chloride, manganese phosphate, sulfuric acid.
  • the cash crop includes tobacco, cotton, camellia, rape, beet, sugar cane, soybean, tea tree, peanut, etc.;
  • the food crop includes wheat, rice, corn, sorghum, etc.;
  • the vegetable includes cabbage, bitter gourd, loofah, celery, Eggplant, tomato, melon, spinach, garlic, onion, pepper, cucumber, spinach, kohlrabi, potato, sweet potato, radish, carrot, pea, kidney bean, green beans, edamame, etc.; and the fruit including mango, watermelon, apple, pear , banana, papaya, citrus, orange, grape, cherry, lychee, peach, plum, apricot, strawberry, pineapple, blueberry, kiwi, jujube, persimmon, cantaloupe, etc., trefoil, variegated crested flower, etc.
  • the economic forest includes: rubber, coconut, walnut, chestnut, hazelnut, etc.
  • the flower includes: one or two-year-old herbal flowers: Dianthus, marigold, Calendula, Nasturtium, Pansy, Impatiens, Violet Jasmine, Corn Poppy, California Poppy, A String of Red, Violet, Morning Glory, Mimosa, Cockscomb, Amaranth, Night Money, Sky Blue Hydrangea, Four Seasons Primula; Perennial Herbal Flowers: Wenzhu, Impatiens, Spearmint , Clivia, Vanilla, Violet, Strelitzia, Mangoslavia, Zhudinghong, Evergreen, Asparagus, Calla Lily, Baizilian, Passiflora, Cyclamen, Gloxinia, Gerbera, Sun Flower, Begonia, Hanging Pineapple Deciduous perennial and bulbous grasses: lily, valerian, peony, chrys
  • Chinese herbal medicines include: Ginseng, Sanqi, Rhubarb, Chuanxiong, Gastrodia elata, Fungus, Muxiang, Ophiopogon japonicus, Bupleurum, Codonopsis, Astragalus, Coptis, Areca, Mint, Strych, Schisandra, Galla Chinensis, Burdock, Cordyceps , Nutmeg, Benzoin, Corydalis, Bilateral Needle, Polygonum, Ban GmbH, Acanthopanax, Mangosteen, Ten Contributions; Types of Turfgrass: Thin Pruning, Fluffy, Prunus, Prunus, Kobre, Tough Leaf purple fescue, purple fescue, fescue, scutellaria and tall fescue, bluegrass, common bluegrass, bluegrass and bluegrass, perennial ryegrass, foxtail, steppe, yarrow , big spiked yarrow, Chinese knotted grass, Manila sedge,
  • the invention uses biologically derived sodium alginate oligosaccharide for amino acid trace element fertilizer, which is green, environmentally friendly, pollution-free, pollution-free and pollution-free.
  • the main active ingredients in the present invention amino acids, trace elements and sodium alginate oligosaccharides, have a wide range of sources and are inexpensive, and are suitable for large-scale promotion.
  • the foliar fertilizer composition of the present invention has a small amount of use, has a long-lasting effect, and does not cause phytotoxicity.
  • the foliar fertilizer composition of the present invention has the dual effects of providing plant nutrition and promoting plant growth, and can partially replace the use of chemical plant growth regulators, reduce chemical residues, and solve food safety problems.
  • the foliar fertilizer composition is added to a solvent, an auxiliary agent, a water-based agent, an emulsifiable concentrate, a soluble liquid agent, an aqueous emulsion, a microemulsion, a suspending agent; a known carrier, an auxiliary agent, a powder, a soluble powder, a wettable powder, and a granule are added.
  • Agent water-dispersible granules, microcapsules, tablets. This embodiment describes a conventional water agent ratio, but the content of the present invention is not limited to this:
  • the amino acid content was determined by an automatic amino acid analyzer; the trace element fertilizer was determined according to the method in the NY1428 annex.
  • the mass of sodium alginate oligosaccharide was determined by an electrospray ionization (ESI) liquid chromatography-mass spectrometer.
  • the chromatographic conditions were: mass spectrometry mode in positive ion mode; electrospray ionization source (ESI); spray voltage 4.5 kV; transfer capillary temperature 350 °C; Sheath gas (N2) pressure 30 au (lau approximately 1 psi) , Aux gas (N2) pressure is 5 au, Ion sweep gas (N2) pressure is 0 au; Scanning range: 150 ⁇ 2000.
  • the content was determined by colorimetry.
  • the electrospray ionization mass spectrum is shown in Figure 1.
  • Example 2 Using the foliar fertilizer composition to increase rapeseed yield
  • Treatment 1 The foliar fertilizer composition of Example 1 was diluted 500 times, sprayed once in the rapeseed period + full bloom period, 30 kg per mu; 2 was not sprayed (control). Each treatment was repeated three times and randomly arranged. The plot length was 13.52 m, the width was 7.4 m, and the row spacing was 0.74 mx0.26 m. Each plot was planted with 11 rows and 583 caves, planted with double plants, and 7226 plants were planted in acre. The spraying time was carried out on March 7 and March 25, 2012, using the Workers and farmers 16 manual sprayer, with the leaf wet and not dripping. The test investigated each treatment during the maturity of rapeseed.
  • the yield experiment was carried out using three different varieties of cherries, red light, pioneer, and dragon crown.
  • the foliar fertilizer group of Example 1 was used.
  • the compound was diluted 500 times, sprayed on the snow before April 12, 2012, and 200 kg of liquid medicine was applied to investigate the yield of 3 cherry varieties.
  • the results showed that the foliar fertilizer composition had a good yield increasing effect on the cherry.
  • the results are shown in Table 3 below.
  • Example 5 Using the foliar fertilizer composition can increase the yield and composition of Nanguo pear
  • the foliar fertilizer composition was diluted with 500 times of liquid, 200 kg of water was applied per mu, and the yield experiment was carried out on pear trees in Pucheng in Shaanxi in early spring.
  • the foliar fertilizer composition was sprayed twice on the pear tree flower before flowering (inflorescence separation period) and after the young flowering period. Investigate production, the specific results are shown in Table 5 below:
  • Example 2 the foliar fertilizer composition of Example 1 was diluted 500 times, and 200 kg of water was applied per mu.
  • the yield experiment was carried out on citrus in Yichang, Hubei.
  • the foliar fertilizer composition was sprayed on the citrus flower before flowering (inflorescence separation period) and after the young flowering period, and sprayed twice.
  • the specific results are shown in Table 6 below:
  • Example 7 Using this foliar fertilizer composition can promote rice rooting and improve drought resistance
  • the drought resistance test of rice seedlings was carried out by using the foliar fertilizer composition of Example 1.
  • the growth of rice seedlings was treated with two leaves and one heart, and the drought conditions were simulated by adding 20% PEG6000.
  • the number of treatments in each group was 100; the leaf fertilizer was diluted 500 times, and sprayed once a week for three times.
  • the results showed that the rice seedlings of the foliar fertilizer composition significantly increased the activity and root number of the untreated components. The details are shown in Table 7 below.
  • Example 8 Using the Foliar Fertilizer Composition to Improve Drought Resistance of Wheat
  • Example 8 Using the foliar fertilizer composition of Example 1 for rooting or foliar application of wheat, 3 g per acre was used, and seven days after sampling, the drought resistance indexes were detected by spectrophotometry. The specific results are shown in Table 8 below:
  • Example 11 Method for arranging foliar fertilizer composition containing sodium alginate oligosaccharide
  • the active ingredients of the foliar fertilizer are amino acids, sodium alginate oligosaccharides and trace elements, wherein the amino acids include, but are not limited to, glycine, alanine, isoleucine, cystine, cysteine, methionine. , threonine, serine, phenylalanine, tyrosine, tryptophan, valine, hydroxyproline, glutamic acid, aspartic acid, etc.; the trace element fertilizer is capable of providing plant trace elements Fertilizers, such as boron fertilizer, molybdenum fertilizer, manganese fertilizer, copper fertilizer, iron fertilizer, zinc fertilizer, etc.
  • Fertilizers such as boron fertilizer, molybdenum fertilizer, manganese fertilizer, copper fertilizer, iron fertilizer, zinc fertilizer, etc.
  • the plant antifreeze and cold resistance agent is added to a solvent, an auxiliary agent, a water preparation agent, an emulsifiable concentrate, a water emulsion, a microemulsion, a suspension agent; a known carrier, an auxiliary agent, a powder, a soluble powder, a wettable powder, and a granule Agent, water-dispersible granules, microcapsules, tablets.
  • This embodiment describes a commonly used liquid dosage ratio, but the content of the present invention is not limited thereto: 100-200 g of amino acid is dissolved in 1 L of water, and then the required trace elements and sodium alginate oligosaccharide are added. Then, the pH is adjusted with a base, and an auxiliary agent is added to completely dissolve it, thereby preparing the foliar fertilizer composition.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Disclosed is a foliar fertilizer composition containing sodium alginate oligosaccharide, the composition comprising sodium alginate oligosaccharide, amino acid, and microelements capable of significantly promoting plant growth. The sodium alginate oligosaccharide, also called sodium alginate, kelp glue or algin, is a natural polysaccharide carbohydrate extracted from kelp, and is a copolymer comprising a-L-mannuronic acid, b-D-guluronic acid, and different GGGMMM segments; the a-L-mannuronic acid and the b-D-guluronic acid are linked by a 1,4-glucosidic bond; the sodium alginate oligosaccharide has a molecular formula of (C6H7O6Na)n, a polymerization degree of 2-10, a mannuronic acid to guluronic acid (M/G) ratio of 7:3, and a uronic acid content of more than 90%, accounting for 2-3% of the total amount; and the microelements comprise 2-20% of the foliar fertilizer, and comprise at least two of sulfur, boron, iron, manganese, copper, zinc, and molybdenum. The composition is mainly applied to economic crops, fruits and vegetables, grain crops, economic forest, flowers, lawns and the like.

Description

含海藻酸钠寡糖的叶面肥组合物及其应用 技术领域  Foliar fertilizer composition containing sodium alginate oligosaccharide and application thereof

本发明涉及到植物的生理代谢, 具体地说是一种以氨基酸、海藻酸钠寡糖和微量 元素为主要活性物质在植物促生长中应用。 背景技术  The present invention relates to physiological metabolism of plants, and more particularly to the use of amino acids, sodium alginate oligosaccharides and trace elements as main active substances in plant growth promotion. Background technique

传统的化学肥料肥效单一、污染严重, 长期使用破坏土壤结构, 而人工合成植物 生长调节剂则易引起餐桌污染, 导致农产品品质下降。 我国加入 WTO后, 食品安全 已经成为影响农业和食品工业在国际市场竞争力的关键因素,无公害农产品安全生产 技术的开发和应用迫在眉睫。氨基酸和海洋寡糖属天然提取物, 可弥补我国传统肥料 和人工合成植物生长调节剂的不足, 作为新型叶面肥开发,有助于提高我国农产品安 全, 保护环境。  Traditional chemical fertilizers have a single fertilizer effect and serious pollution. The long-term use destroys the soil structure, while the artificial plant growth regulators are likely to cause table pollution, resulting in a decline in the quality of agricultural products. After China's accession to the WTO, food safety has become a key factor affecting the competitiveness of agriculture and food industry in the international market. The development and application of safe production technology for pollution-free agricultural products is extremely urgent. The natural extracts of amino acids and marine oligosaccharides can make up for the deficiency of traditional fertilizers and synthetic plant growth regulators in China. As a new type of foliar fertilizer development, it can help improve the safety of agricultural products in China and protect the environment.

壳寡糖等一些寡糖类物质从上世纪 70年代被应用于植物领域, 具有诱导植物抗 性、促进植物生长等功能, 这类寡糖物质被专门命名为寡糖素。海藻酸钠寡糖是寡糖 素的重要分子之一,是通过酶解海藻酸钠得到的由 a-L-甘露糖醛酸 (mannuronic acid, M)与 b-D-古罗糖醛酸 (guluronic acid, G) 依靠 1, 4-糖苷键连接并由不同 GGGMMM 片段组成的低聚糖, 具有相对分子质量小、 毒性低、 水溶性好、 吸收性强等优点。 含 有以海藻酸钠寡糖为活性成份的氨基酸微量元素肥未见文献和专利报道。 发明内容  Some oligosaccharides such as chitooligosaccharides have been used in the field of plants since the 1970s, and have functions such as inducing plant resistance and promoting plant growth. Such oligosaccharides are specifically named as oligosaccharides. Alginate oligosaccharide is one of the important molecules of oligosaccharide, which is obtained by enzymatic hydrolysis of sodium alginate from aL-mannuronic acid (M) and bD-guluronic acid (G). An oligosaccharide which is linked by a 1,4-glycosidic bond and composed of different GGGMMM fragments has the advantages of relatively low molecular weight, low toxicity, good water solubility, and strong absorption. Amino acid trace element fertilizer containing sodium alginate oligosaccharide as active ingredient has not been reported in the literature and patents. Summary of the invention

本发明的目的在于提供一种含有海藻酸钠寡糖为活性成分的氨基酸微量元素肥 料。  It is an object of the present invention to provide an amino acid trace element fertilizer containing sodium alginate oligosaccharide as an active ingredient.

为实现上述目的, 本发明采用的技术方案为:  In order to achieve the above object, the technical solution adopted by the present invention is:

一种氨基酸微量元素叶面肥,所述叶面肥的主要活性成份为氨基酸、微量元素和 海藻酸钠寡糖, 其余成分为溶剂、 助剂或载体。 该叶面肥组合物可用于经济作物、 粮 食作物、 蔬菜、 果树、 经济林木、 花卉及草坪等。 开花结果类植物保花保果率达到 50-99%, 增加植株干旱条件下抗性相关的各种酶类和次生代谢物的合成 70%-90%。  An amino acid trace element foliar fertilizer, wherein the main active ingredients of the foliar fertilizer are amino acids, trace elements and sodium alginate oligosaccharides, and the remaining components are solvents, adjuvants or carriers. The foliar fertilizer composition can be used for cash crops, food crops, vegetables, fruit trees, economic forests, flowers and lawns. The flowering and fruit-preserving rate of flowering plants reached 50-99%, increasing the synthesis of various enzymes and secondary metabolites related to resistance under drought conditions of plants 70%-90%.

所述的活性成份: 所述氨基酸包含甘氨酸、 丙氨酸、 异亮氨酸、 胱氨酸、 半胱氨 酸、 甲硫氨酸、 苏氨酸、 丝氨酸、 苯丙氨酸、 酪氨酸、 色氨酸、 脯氨酸、 羟脯氨酸、 谷氨酸、天门冬氨酸等中的几种;所述微量元素肥料为能够提供植物微量元素的肥料, 如硼肥、 钼肥、 锰肥、 铜肥、 铁肥、 锌肥等; 所述海藻酸钠寡糖为海带中提取的天然 多糖碳水化合物,由 a-L-甘露糖醛酸 (mannuronic acid, M)与 b-D-古罗糖醛酸 (guluronic acid, GM衣靠 1, 4-糖苷键连接并由不同 GGGMMM 片段组成的共聚物, 分子式为 (C6H706Na)n, 聚合度 2-10, 糖醛酸组成为 M/G=7:3, 糖醛酸含量 >90%。 所述叶面肥 组合物稀释 500倍液, 于作物花蕾期叶面喷施, 7-10天喷施 1次, 连续喷施 3-4次。 所述活性组分加入溶剂、 分散剂和助剂配成水剂、 乳油、 可溶性液剂、 水乳剂、 微乳剂、悬浮剂。所述溶剂包括但不局限于: 水、 乙醇、 丙酮、 二甲苯、 甲苯、 氯苯、 环己烷、 甲醇、 二甲基甲酰胺、 吡咯烷酮、 环己酮、 丁醇、 戊醇、 醚类、 酯类、 二甲 基亚砜、 月桂醇、 二氯甲烷、 石油馏出物的一种或上述混合物, 溶剂质量比例为 10-99%; 所述助剂有非离子型和离子型, 包括但不局限于: 冰醋酸、 盐酸、 硫酸、 氢氧化钠、 氢氧化钾、 氨水、 脂肪醇聚氧乙烯醚、 烷基酚聚氧乙烯醚、 聚氧乙烯脂肪 酸酯、 烷基芳基聚二醇醚、 烷基磺酸盐、 芳基磺酸盐、 脂肪酸聚二乙醇、 十二烷基苯 磺酸盐、烷基酚聚氧乙烯醚磷酸酯、聚氧乙烯烷基酚甲醛缩合物等的一种或者混合物。 助剂质量比例为 10-99%。 所述分散剂包括木质素磺酸盐、 甲基纤维素、 烷基磺酸盐、 烷基萘磺酸盐、 脂肪醇聚氧乙烯醚等的一种或者上述混合物, 质量比例为 0.05-50%; 所述叶面肥组合物可以加入硫酸锌、硝酸锌、氯化锌、磷酸锌、硫酸铜、硝酸铜、 氯化铜、 磷酸铜、 硝酸锰、 硫酸锰、 氯化锰、 磷酸锰、 硫酸钾、 硝酸钾、 磷酸钾、 硫 酸二氢钾、 磷酸氢二钾、 氯化钾、 硫酸镁、 硝酸镁、 氯化镁、 硫酸亚铁、 硫酸铁、 硝 酸亚铁、 硝酸铁、 氯化亚铁、 氯化铁、 磷酸亚铁和磷酸铁的一种或者混合物, 质量比 例为 0.005-20%。 The active ingredient: the amino acid comprises glycine, alanine, isoleucine, cystine, cysteine, methionine, threonine, serine, phenylalanine, tyrosine, Several of tryptophan, proline, hydroxyproline, glutamic acid, aspartic acid, etc.; the trace element fertilizer is a fertilizer capable of providing plant trace elements, such as boron fertilizer, molybdenum fertilizer, manganese fertilizer , copper fertilizer, iron fertilizer, zinc fertilizer, etc.; the sodium alginate oligosaccharide is a natural polysaccharide carbohydrate extracted from kelp, by aL-mannuronic acid (M) and bD-guluronic acid ( Guluronic acid, GM is a copolymer consisting of 1, 4-glycosidically linked and composed of different GGGMMM fragments, with a molecular formula of (C 6 H 7 0 6 Na)n, a degree of polymerization of 2-10, and a uronic acid composition of M/G. =7:3, uronic acid content >90%. The composition is diluted 500 times, sprayed on the foliage of the flower bud stage, sprayed once every 7-10 days, and sprayed 3-4 times continuously. The active component is added to a solvent, a dispersing agent and an auxiliary agent to form a water agent, an emulsifiable concentrate, a soluble liquid agent, a water emulsion, a microemulsion, and a suspending agent. The solvent includes, but is not limited to: water, ethanol, acetone, xylene, toluene, chlorobenzene, cyclohexane, methanol, dimethylformamide, pyrrolidone, cyclohexanone, butanol, pentanol, ethers, One or a mixture of esters, dimethyl sulfoxide, lauryl alcohol, dichloromethane, petroleum distillate, a solvent mass ratio of 10-99%; the auxiliary agent has a nonionic type and an ionic type, including but Not limited to: glacial acetic acid, hydrochloric acid, sulfuric acid, sodium hydroxide, potassium hydroxide, ammonia, fatty alcohol polyoxyethylene ether, alkylphenol ethoxylate, polyoxyethylene fatty acid ester, alkyl aryl polyglycol One of an ether, an alkyl sulfonate, an aryl sulfonate, a fatty acid polydiethanol, a dodecylbenzene sulfonate, an alkylphenol ethoxylate phosphate, a polyoxyethylene alkylphenol formaldehyde condensate, and the like Species or mixtures. The mass ratio of additives is 10-99%. The dispersing agent includes one or a mixture of the above-mentioned lignosulfonate, methylcellulose, alkylsulfonate, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether, etc., and the mass ratio is 0.05-50%. The foliar fertilizer composition may be added with zinc sulfate, zinc nitrate, zinc chloride, zinc phosphate, copper sulfate, copper nitrate, copper chloride, copper phosphate, manganese nitrate, manganese sulfate, manganese chloride, manganese phosphate, sulfuric acid. Potassium, potassium nitrate, potassium phosphate, potassium dihydrogen sulfate, dipotassium hydrogen phosphate, potassium chloride, magnesium sulfate, magnesium nitrate, magnesium chloride, ferrous sulfate, iron sulfate, ferrous nitrate, ferric nitrate, ferrous chloride, chlorine One or a mixture of iron, ferrous phosphate and iron phosphate in a mass ratio of 0.005-20%.

所述经济作物包括烟草、棉花、 油茶、 油菜、 甜菜、甘蔗、 大豆、 茶树、花生等; 所述粮食作物包括小麦、 水稻、 玉米、 高粱等; 所述蔬菜包括白菜、 苦瓜、 丝瓜、 芹 菜、 茄子、 西红柿、 冬瓜、 菠菜、 大蒜、 葱、 辣椒、 黄瓜、 菠菜、 大头菜、 马铃薯、 番薯、 萝卜、 胡萝卜、 豌豆、 菜豆、 四季豆、 毛豆等; 和所述水果包括芒果、 西瓜、 苹果、 梨、 香蕉、 木瓜、 柑橘、 橙子、 葡萄、 樱桃、 荔枝、 桃、 李、 杏、 草莓、 菠萝、 蓝莓、 猕猴桃、 枣、 柿子、 哈密瓜等, 三叶、 多变小冠花等、 匍匐马蹄金、 沿阶草、 百里香、 匍匐委陵菜等。 所述经济林木包括: 橡胶、 椰子、 核桃、 板栗、 榛子等, 所 述花卉包括有: 一、 二年生草本花卉: 石竹、 万寿菊、 金盏菊、 旱金莲、 三色堇、 凤 仙花、 紫茉莉、 虞美人、 花菱草、 一串红、 紫罗兰、 牵牛花、 含羞草、 鸡冠花、 千日 红、 夜落金钱、 天蓝绣球、 四季报春; 多年生草本花卉: 文竹、凤仙、 吊兰、君子兰、 香子兰、 紫罗兰、 鹤望兰、 文殊兰、 朱顶红、 万年青、 天门冬、 马蹄莲、 百子莲、 西 番莲、 仙客来、 大岩桐、 非洲菊、 太阳花、 秋海棠、 垂花凤梨; 落叶宿根和球根类草 花: 百合、 萱草、 牡丹、 菊花、 芍药、 鸢尾、 水仙、 蜀葵、 大丽花、 唐菖蒲、 小苍兰、 晚香玉、 郁金香、 风信子、 美人蕉; 水生花卉: 荷花、 睡莲、 凤眼莲、 石菖蒲; 蕨类花卉: 肾蕨、 铁线蕨、 鹿角蕨; 兰科花卉: 春兰、 蕙兰、 建兰、 墨兰、 台兰、 寒兰、 石斛、 兔耳兰、 卡特兰、 香港兜兰; 常绿灌木类: 米兰、 栀子、 含笑、 茉莉、 扶桑、 探春、 棕竹、 一品红、 五色梅、 夜丁香、 六月雪、 迎春花、 素馨花、 夹竹桃、 南天竹、 杜鹃花、 大叶黄杨; 落叶灌木类: 月季、 蔷薇、 玫瑰、 腊梅、 石榴、 迎春、 木槿、 地棠、 黄刺玫、 珍珠梅、 榆叶梅、 紫丁香、 木芙蓉、 无花果、 贴梗海棠、 木本 象牙红; 常绿乔木类: 苏铁、 山棕、 蒲葵、 榕树、 冬青、 桂花、、 山茶、 佛手、 香圆、 金橘、 翠柏、 龙柏、 橡皮树、 广玉兰、 五针松、 金钱松、 罗汉松、 雪松、 白兰花; 落 叶乔木类: 桃花、 梅花、 樱花、 玉兰、 紫荆、 海棠花、 垂丝海棠; 常绿藤本类: 龙吐 珠、 龟背竹、 叶子花、 常春藤、 万字茉莉、 非洲凌宵; 落叶藤本类: 凌宵、 藤本、 木 香、 金银花、 爬山虎; 仙人掌与多肉植物: 昙花、 芦荟、 景天、 蟹爪兰、 龙舌兰、 虎 皮兰、 虎刺梅、 霸王鞭、 仙人球类、 令箭荷花、 落地生根、 木叶仙人掌。 中药材类包 括: 人参、 三七、 大黄、 川芎、 天麻、 木耳、 木香、 麦冬、 柴胡、 党参、 黄芪、 黄 连、 槟榔、 薄荷、 马钱子、 五味子、 五倍子、 牛蒡子、 冬虫草、 肉豆蔻、 安息香、 延 胡索、 两面针、 何首乌、 板蓝根、 刺五加、 罗汉果、 十大功劳; 草坪草的种类: 细弱 剪股颖、 绒毛剪股颖、 匍匐剪股颖、 小糠草, 韧叶紫羊茅、 匍匐紫羊茅、 羊茅、 细叶 茅和高羊茅等, 草地早熟禾、普通早熟禾、林地早熟禾和早熟禾,多年生黑麦草、洋狗 尾草、 梯牧草、 结缕草、 大穗结缕草、 中华结缕草、 马尼拉结缕草、 细叶结缕草、 白 颖苔草、 细叶苔、 异穗苔和卵穗苔草等; 白三叶、 红三叶、 多变小冠花等、 匍匐马蹄 金、 沿阶草、 百里香、 匍匐委陵菜等。 The cash crop includes tobacco, cotton, camellia, rape, beet, sugar cane, soybean, tea tree, peanut, etc.; the food crop includes wheat, rice, corn, sorghum, etc.; the vegetable includes cabbage, bitter gourd, loofah, celery, Eggplant, tomato, melon, spinach, garlic, onion, pepper, cucumber, spinach, kohlrabi, potato, sweet potato, radish, carrot, pea, kidney bean, green beans, edamame, etc.; and the fruit including mango, watermelon, apple, pear , banana, papaya, citrus, orange, grape, cherry, lychee, peach, plum, apricot, strawberry, pineapple, blueberry, kiwi, jujube, persimmon, cantaloupe, etc., trefoil, variegated crested flower, etc. Along the grass, thyme, 匍匐 匍匐 菜. The economic forest includes: rubber, coconut, walnut, chestnut, hazelnut, etc., the flower includes: one or two-year-old herbal flowers: Dianthus, marigold, Calendula, Nasturtium, Pansy, Impatiens, Violet Jasmine, Corn Poppy, California Poppy, A String of Red, Violet, Morning Glory, Mimosa, Cockscomb, Amaranth, Night Money, Sky Blue Hydrangea, Four Seasons Primula; Perennial Herbal Flowers: Wenzhu, Impatiens, Spearmint , Clivia, Vanilla, Violet, Strelitzia, Mangoslavia, Zhudinghong, Evergreen, Asparagus, Calla Lily, Baizilian, Passiflora, Cyclamen, Gloxinia, Gerbera, Sun Flower, Begonia, Hanging Pineapple Deciduous perennial and bulbous grasses: lily, valerian, peony, chrysanthemum, peony, iris, narcissus, hollyhock, dahlia, gladiolus, freesia, tuberose, tulip, hyacinth, canna; aquatic flowers: lotus, water lily , Eichhornia crassipes, Shichangpu; Fern flowers: Kidney fern, Adiantum, Staghorn fern; Orchid flower: Chunlan, Cymbidium, Jianlan, Molan, Tailan, Hanlan, Shijie, Rabbit Ear, Cattleya, Hong Kong Paphiopedilum; Evergreen shrubs: Milan, scorpion, smiling, jasmine, hibiscus, spring, brown bamboo, poinsettia , five-color plum, night clove, June snow, winter jasmine, frangipani, oleander, Nandina, azalea, big leaf boxwood; deciduous shrubs: rose, rose, rose, wax, pomegranate, spring, hibiscus, earth棠, yellow thorn rose, pearl plum, eucalyptus, lilac, wood hibiscus, fig, stalked sea bream, woody Ivory red; evergreen trees: cycad, mountain brown, palm, eucalyptus, holly, sweet-scented osmanthus, camellia, bergamot, fragrant, kumquat, cypress, cypress, rubber tree, magnolia, pine, pine , Podocarpus, cedar, white orchid; deciduous trees: peach, plum, cherry, magnolia, bauhinia, jellyfish, squid; evergreen vines: dragon spit, monstera, bougainvillea, ivy, 4D Jasmine, African scorpion; deciduous vines: scorpion, vine, woody, honeysuckle, ivy; cactus and succulents: scented flowers, aloe vera, sedum, crab claws, agave, tiger skin, tiger thorn, Tyrannosaurus, Prickly Pear, Lotus Flower, Rooting, Wood Leaf Cactus. Chinese herbal medicines include: Ginseng, Sanqi, Rhubarb, Chuanxiong, Gastrodia elata, Fungus, Muxiang, Ophiopogon japonicus, Bupleurum, Codonopsis, Astragalus, Coptis, Areca, Mint, Strych, Schisandra, Galla Chinensis, Burdock, Cordyceps , Nutmeg, Benzoin, Corydalis, Bilateral Needle, Polygonum, Banlangen, Acanthopanax, Mangosteen, Ten Contributions; Types of Turfgrass: Thin Pruning, Fluffy, Prunus, Prunus, Kobre, Tough Leaf purple fescue, purple fescue, fescue, scutellaria and tall fescue, bluegrass, common bluegrass, bluegrass and bluegrass, perennial ryegrass, foxtail, steppe, yarrow , big spiked yarrow, Chinese knotted grass, Manila sedge, fineleaf sedge, white sedge, fine leaf moss, sphagnum moss and egg sedge; white clover, red clover, changeable Small crown flowers, etc., 匍匐 horseshoe gold, along the grass, thyme, 匍匐 匍匐 菜.

本发明具有如下优点:  The invention has the following advantages:

1 . 本发明将生物来源的海藻酸钠寡糖用于氨基酸微量元素肥料, 绿色、 环保、 无公害、 无污染、 无公害。  1. The invention uses biologically derived sodium alginate oligosaccharide for amino acid trace element fertilizer, which is green, environmentally friendly, pollution-free, pollution-free and pollution-free.

2. 本发明中的主要活性成份氨基酸、 微量元素和海藻酸钠寡糖, 来源广泛, 价 格低廉, 适于大规模推广。  2. The main active ingredients in the present invention, amino acids, trace elements and sodium alginate oligosaccharides, have a wide range of sources and are inexpensive, and are suitable for large-scale promotion.

3. 本发明中的叶面肥组合物使用量少, 持效时间长, 且不会产生药害。  3. The foliar fertilizer composition of the present invention has a small amount of use, has a long-lasting effect, and does not cause phytotoxicity.

4. 本发明中的叶面肥组合物具有提供植物营养和促进植株生长的双重功效, 可 部分替代化学植物生长调节剂的使用, 降低化学残留, 解决食品安全问题。 附图说明  4. The foliar fertilizer composition of the present invention has the dual effects of providing plant nutrition and promoting plant growth, and can partially replace the use of chemical plant growth regulators, reduce chemical residues, and solve food safety problems. DRAWINGS

图 1. 海藻酸钠寡糖的电喷物电离质谱图谱。 具体实施方式  Figure 1. Electrospray ionization mass spectrometry of sodium alginate oligosaccharides. detailed description

下面结合实施实例对本发明做进一步说明。  The invention will be further described below in conjunction with the examples.

实施例 1 常用的本叶面肥组合物的配制  Example 1 Preparation of a commonly used foliar fertilizer composition

本叶面肥组合物加入溶剂、助剂配成水剂、乳油、可溶性液剂、水乳剂、微乳剂、 悬浮剂; 加入公知的载体、 助剂配成粉剂、 可溶性粉剂、 可湿性粉剂、 颗粒剂、 水分 散性粒剂、 微胶囊剂、 片剂。 本实施例介绍了一种常用的水剂配比, 但本发明的内容 并不局限于此:  The foliar fertilizer composition is added to a solvent, an auxiliary agent, a water-based agent, an emulsifiable concentrate, a soluble liquid agent, an aqueous emulsion, a microemulsion, a suspending agent; a known carrier, an auxiliary agent, a powder, a soluble powder, a wettable powder, and a granule are added. Agent, water-dispersible granules, microcapsules, tablets. This embodiment describes a conventional water agent ratio, but the content of the present invention is not limited to this:

将 25g海藻酸钠寡糖溶于 1L水中, 然后同时加入终质量浓度 16%的硼酸、 终质 量浓度 12%的硼砂, 最后加入终质量浓度 0.4%的钼酸铵和 106 g含量 95%的氨基酸 (主要包括苏氨酸、 天冬氨酸、 丝氨酸、 谷氨酸、 脯氨酸、 精氨酸、 甘氨酸等), 用 KOH调节 pH 6.5, 使其完全溶解, 从而制备出本叶面肥组合物。 25 g of sodium alginate oligosaccharide was dissolved in 1 L of water, and then a final concentration of 16% boric acid, a final concentration of 12% borax, and finally a final concentration of 0.4% ammonium molybdate and 106 g of 95% amino acid were added. (mainly including threonine, aspartic acid, serine, glutamic acid, valine, arginine, glycine, etc.), the pH is 6.5 adjusted with KOH, and completely dissolved, thereby preparing the foliar fertilizer composition .

其中氨基酸含量用氨基酸自动分析仪检测;微量元素肥料按 NY1428附件中的方 法测定。 其中海藻酸钠寡糖的质量由配有电喷雾离子源(ESI)液相色谱 -质谱联用仪 测定。 色谱测定条件为: 质谱模式为正离子模式; 电喷雾离子源 (ESI ); 喷雾电压 4.5 kV; 传输毛细管温度 350°C; 鞘气(Sheath gas, N2)压力为 30 au ( lau约为 lpsi), 辅助气 (Aux gas, N2) 压力为 5 au, 离子吹扫气 (Ion sweep gas, N2) 压力为 0 au; 扫描范围: 150〜2000。 含量采用比色法测定。 其电喷物电离质谱图谱见图 1。  The amino acid content was determined by an automatic amino acid analyzer; the trace element fertilizer was determined according to the method in the NY1428 annex. The mass of sodium alginate oligosaccharide was determined by an electrospray ionization (ESI) liquid chromatography-mass spectrometer. The chromatographic conditions were: mass spectrometry mode in positive ion mode; electrospray ionization source (ESI); spray voltage 4.5 kV; transfer capillary temperature 350 °C; Sheath gas (N2) pressure 30 au (lau approximately 1 psi) , Aux gas (N2) pressure is 5 au, Ion sweep gas (N2) pressure is 0 au; Scanning range: 150~2000. The content was determined by colorimetry. The electrospray ionization mass spectrum is shown in Figure 1.

实施例 2 使用本叶面肥组合物提高油菜产量  Example 2 Using the foliar fertilizer composition to increase rapeseed yield

试验共设计两个处理: 处理 1, 将实施例 1叶面肥组合物稀释 500倍, 于油菜抽 薹期 +盛花期各喷一次, 亩施 30 kg; 处理 2不喷药 (对照)。 每处理重复三次, 随机 排列, 小区长 13.52 m, 宽 7.4 m, 行穴距 0.74 mx0.26 m, 每小区栽 11行, 583穴, 双株栽植, 亩栽 7226株。 喷药时间于 2012年 3月 7日和 3月 25日进行, 使用工农 16 型手动喷雾器, 以叶湿不滴水为度。 试验在油菜成熟期对各处理进行调查。 每小 区按 4个点取样, 每点调查 50株, 调查指标有总角果树、 每角果粒树、 千粒重和实 际产量。 实验结果显示, 施用本叶面肥组合物能显著提高油菜硼含量、产量及其构成 因子。 具体如下表 1和表 2所示。  A total of two treatments were designed for the test: Treatment 1. The foliar fertilizer composition of Example 1 was diluted 500 times, sprayed once in the rapeseed period + full bloom period, 30 kg per mu; 2 was not sprayed (control). Each treatment was repeated three times and randomly arranged. The plot length was 13.52 m, the width was 7.4 m, and the row spacing was 0.74 mx0.26 m. Each plot was planted with 11 rows and 583 caves, planted with double plants, and 7226 plants were planted in acre. The spraying time was carried out on March 7 and March 25, 2012, using the Workers and Farmers 16 manual sprayer, with the leaf wet and not dripping. The test investigated each treatment during the maturity of rapeseed. Each sub-region was sampled at 4 points, and 50 strains were surveyed at each point. The survey indicators included total pod, pericarp, 1000-grain weight, and actual yield. The experimental results show that the application of the foliar fertilizer composition can significantly increase the boron content, yield and its constituent factors of rapeseed. The details are shown in Table 1 and Table 2 below.

表 1. 使用本叶面肥组合物对油菜抽薹期干物重及 B利用效率的影响  Table 1. Effect of using this foliar fertilizer composition on dry matter weight and B utilization efficiency in rapeseed

Figure imgf000005_0001
表 2. 使用本叶面肥组合物对油菜产量及其构成因子的影响
Figure imgf000005_0001
Table 2. Effect of using this foliar fertilizer composition on rapeseed yield and its constituent factors

Figure imgf000005_0002
实施例 3 使用本叶面肥组合物提高樱桃产量
Figure imgf000005_0002
Example 3 Using this foliar fertilizer composition to increase cherry yield

利用红灯、先锋、 龙冠三种不同品种的樱桃进行产量实验, 将实施例 1叶面肥组 合物稀释 500倍, 于 2012年 4月 12号雪前喷施, 亩施 200kg药液, 调查 3个樱桃品 种产量, 结果显示, 本叶面肥组合物对樱桃具有较好的增产效果, 具体结果如下表 3 所示。 The yield experiment was carried out using three different varieties of cherries, red light, pioneer, and dragon crown. The foliar fertilizer group of Example 1 was used. The compound was diluted 500 times, sprayed on the snow before April 12, 2012, and 200 kg of liquid medicine was applied to investigate the yield of 3 cherry varieties. The results showed that the foliar fertilizer composition had a good yield increasing effect on the cherry. The results are shown in Table 3 below.

表 3. 使用本叶面肥组合物对樱桃产量的影响  Table 3. Effect of using this foliar fertilizer composition on cherry yield

Figure imgf000006_0001
实施例 4 使用本叶面肥组合物提高苹果产量
Figure imgf000006_0001
Example 4 Using this foliar fertilizer composition to increase apple yield

2012年 4月 10号, 在陕西洛川富县针对苹果进行产量实验, 将本叶面肥组合物 稀释 500倍, 喷施苹果树, 亩施 200 kg的药液。 调查产量和外观品质, 具体结果如 下表 4所示。  On April 10, 2012, in the production experiment of apples in Fuxian County, Luochuan, Shaanxi, the foliar fertilizer composition was diluted 500 times, sprayed with apple trees, and 200 kg of liquid was applied per mu. The yield and appearance quality were investigated, and the specific results are shown in Table 4 below.

表 4. 使用本叶面肥组合物对苹果产量及外观品质的影响  Table 4. Effect of using this foliar fertilizer composition on apple yield and appearance quality

Figure imgf000006_0002
实施例 5 使用本叶面肥组合物能提高南果梨产量及其构成因子
Figure imgf000006_0002
Example 5 Using the foliar fertilizer composition can increase the yield and composition of Nanguo pear

2012年用实施例 1叶面肥组合物稀释 500倍液, 每亩施用水剂 200 kg, 在陕西 蒲城于早春对梨树进行产量实验。在梨树花在开花前(花序分离期)和谢花后幼果期 喷施该叶面肥组合物, 共喷施两次。 调查产量, 具体结果如下表 5所示:  In 2012, the foliar fertilizer composition was diluted with 500 times of liquid, 200 kg of water was applied per mu, and the yield experiment was carried out on pear trees in Pucheng in Shaanxi in early spring. The foliar fertilizer composition was sprayed twice on the pear tree flower before flowering (inflorescence separation period) and after the young flowering period. Investigate production, the specific results are shown in Table 5 below:

表 5. 使用本叶面肥组合物对南果梨产量及产量构成因子的影响 处理药剂组合 单果重 (g) 单株产量 (kg/株) 对照 42. 6 12. 4 叶面肥组合物 57.8 21.6 实施例 6 使用本叶面肥组合物能提高南柑橘产量及其构成因子Table 5. Effect of using this foliar fertilizer composition on the yield and yield components of Nanguo Pear. Treatment combination of single fruit weight (g) Yield per plant (kg/plant) Control 42. 6 12. 4 Foliar fertilizer composition 57.8 21.6 Example 6 Using this foliar fertilizer composition can increase the yield and composition of southern citrus

2012年将实施例 1叶面肥组合物稀释 500倍液, 每亩施用水剂 200 kg, 在湖北 宜昌对柑橘进行了产量实验。在柑橘花在开花前(花序分离期)和谢花后幼果期喷施 该叶面肥组合物, 共喷施两次。 具体结果如下表 6所示: In 2012, the foliar fertilizer composition of Example 1 was diluted 500 times, and 200 kg of water was applied per mu. The yield experiment was carried out on citrus in Yichang, Hubei. The foliar fertilizer composition was sprayed on the citrus flower before flowering (inflorescence separation period) and after the young flowering period, and sprayed twice. The specific results are shown in Table 6 below:

表 6 使用本叶面肥组合物能提高南果梨产量及其构成因子  Table 6 Using this foliar fertilizer composition can increase the yield and composition of Nanguo Pear

Figure imgf000007_0001
实施例 7 使用本叶面肥组合物能促进水稻发根和提高抗旱性
Figure imgf000007_0001
Example 7 Using this foliar fertilizer composition can promote rice rooting and improve drought resistance

利用实施例 1叶面肥组合物对水稻秧苗进行抗旱性实验,水稻秧苗生长为两叶一 心时处理, 干旱条件用添加 20%的 PEG6000模拟。 各组处理数量均为 100株; 本叶 面肥稀释 500倍液使用, 每周喷施 1次, 一共三次。 结果显示: 本叶面肥组合物的水 稻秧苗较未处理组成活率和发根数显著提高。 具体如下表 7所示。  The drought resistance test of rice seedlings was carried out by using the foliar fertilizer composition of Example 1. The growth of rice seedlings was treated with two leaves and one heart, and the drought conditions were simulated by adding 20% PEG6000. The number of treatments in each group was 100; the leaf fertilizer was diluted 500 times, and sprayed once a week for three times. The results showed that the rice seedlings of the foliar fertilizer composition significantly increased the activity and root number of the untreated components. The details are shown in Table 7 below.

表 7. 使用本叶面肥组合物对水稻秧苗成活率和发根数的影响  Table 7. Effect of using this foliar fertilizer composition on survival rate and root number of rice seedlings

Figure imgf000007_0002
实施例 8 使用本叶面肥组合物提高小麦的抗旱性
Figure imgf000007_0002
Example 8 Using the Foliar Fertilizer Composition to Improve Drought Resistance of Wheat

使用实施例 1叶面肥组合物对小麦进行灌根处理或叶面喷施处理, 每亩使用 3g, 七天后取样利用分光光度法检测各抗旱指标。 具体结果如下表 8所示:  Using the foliar fertilizer composition of Example 1 for rooting or foliar application of wheat, 3 g per acre was used, and seven days after sampling, the drought resistance indexes were detected by spectrophotometry. The specific results are shown in Table 8 below:

表 8.干旱条件下使用稀释 500倍液的本叶面肥组合物对小麦抗旱指标的影响  Table 8. Effect of this foliar fertilizer composition diluted with 500 times solution on drought resistance index of wheat under drought conditions

Figure imgf000007_0003
脯氨酸 g/(g FW)) 0.138 0.146 0.128 可溶性糖 ^mol/L) 0.149 0.123 0.105 实施例 9 使用本叶面肥组合物对花卉抗旱性的提高
Figure imgf000007_0003
Proline g/(g FW)) 0.138 0.146 0.128 Soluble sugar ^mol/L) 0.149 0.123 0.105 Example 9 Improvement of drought resistance of flowers using the foliar fertilizer composition

2012年对蝴蝶兰、 仙客来进行了抗旱性实验。 干旱条件用添加 20%的 PEG6000 模拟, 选取蝴蝶兰、 仙客来各 100株进行叶面喷施实施例 1 叶面肥组合物稀释 500 倍液处理, 每周喷施 1次, 一共三次, 具体结果如下表 9所示。  In 2012, drought resistance experiments were carried out on Phalaenopsis and Cyclamen. The drought conditions were simulated by adding 20% PEG6000. 100 strains of Phalaenopsis and Cyclamen were selected for foliar application. The foliar fertilizer composition of Example 1 was diluted 500 times, and sprayed once a week for three times. The results are shown in Table 9 below.

表 9. 使用本叶面肥组合物对花卉抗旱性的影响  Table 9. Effect of using this foliar fertilizer composition on drought resistance of flowers

Figure imgf000008_0001
实施例 10 使用本叶面肥组合物对白羊草抗干旱性的提高
Figure imgf000008_0001
Example 10 Improvement of Drought Resistance of White Sheep Grass Using the Foliar Fertilizer Composition

2012年用实施例 1 叶面肥组合物对白羊草进行了抗旱性实验。 干旱处理用添加 20%的 PEG6000模拟, 叶面喷施本叶面肥组合物稀释 500倍液处理, 每周喷施 1次, 一共五次。 具体结果如下表 10所示:  In 2012, the drought resistance test was carried out on the white sheep grass using the foliar fertilizer composition. The drought treatment was simulated by adding 20% PEG6000, and the foliar spray application of the foliar fertilizer composition was diluted 500 times, and sprayed once a week for five times. The specific results are shown in Table 10 below:

表 10. 使用本叶面肥组合物对白羊草抗旱性的影响

Figure imgf000008_0002
Table 10. Effect of using this foliar fertilizer composition on drought resistance of Leymus chinensis
Figure imgf000008_0002

实施例 11 含海藻酸钠寡糖的叶面肥组合物的配置方法  Example 11 Method for arranging foliar fertilizer composition containing sodium alginate oligosaccharide

本叶面肥的活性成分为氨基酸、海藻酸钠寡糖和微量元素,其中氨基酸包括但不 局限于甘氨酸、 丙氨酸、异亮氨酸、胱氨酸、半胱氨酸、 甲硫氨酸、 苏氨酸、 丝氨酸、 苯丙氨酸、 酪氨酸、 色氨酸、 脯氨酸、 羟脯氨酸、 谷氨酸、 天门冬氨酸等; 所述微量 元素肥料为能够提供植物微量元素的肥料, 如硼肥、 钼肥、 锰肥、 铜肥、 铁肥、 锌肥 等。 本植物防冻抗寒剂加入溶剂、 助剂配成水剂、 乳油、 可溶性液剂、 水乳剂、 微乳 剂、 悬浮剂; 加入公知的载体、 助剂配成粉剂、 可溶性粉剂、 可湿性粉剂、 颗粒剂、 水分散性粒剂、 微胶囊剂、 片剂。 本实施例介绍了一种常用的水剂配比, 但本发明的 内容并不局限于此: 将 100-200 g的氨基酸溶于 1L水中, 然后加入所需要的微量元 素和海藻酸钠寡糖, 然后用碱调节 pH, 加入助剂, 使其完全溶解, 从而制备出本叶 面肥组合物。  The active ingredients of the foliar fertilizer are amino acids, sodium alginate oligosaccharides and trace elements, wherein the amino acids include, but are not limited to, glycine, alanine, isoleucine, cystine, cysteine, methionine. , threonine, serine, phenylalanine, tyrosine, tryptophan, valine, hydroxyproline, glutamic acid, aspartic acid, etc.; the trace element fertilizer is capable of providing plant trace elements Fertilizers, such as boron fertilizer, molybdenum fertilizer, manganese fertilizer, copper fertilizer, iron fertilizer, zinc fertilizer, etc. The plant antifreeze and cold resistance agent is added to a solvent, an auxiliary agent, a water preparation agent, an emulsifiable concentrate, a water emulsion, a microemulsion, a suspension agent; a known carrier, an auxiliary agent, a powder, a soluble powder, a wettable powder, and a granule Agent, water-dispersible granules, microcapsules, tablets. This embodiment describes a commonly used liquid dosage ratio, but the content of the present invention is not limited thereto: 100-200 g of amino acid is dissolved in 1 L of water, and then the required trace elements and sodium alginate oligosaccharide are added. Then, the pH is adjusted with a base, and an auxiliary agent is added to completely dissolve it, thereby preparing the foliar fertilizer composition.

Claims

权 利 要 求 书 Claim 1 . 含海藻酸钠寡糖的叶面肥组合物, 其特征在于: 所述叶面肥的主要活性成份 为氨基酸、 微量元素和海藻酸钠寡糖, 其余成分为溶剂、 助剂或载体。 A foliar fertilizer composition comprising sodium alginate oligosaccharide, characterized in that: the main active ingredients of the foliar fertilizer are amino acids, trace elements and sodium alginate oligosaccharides, and the remaining components are solvents, adjuvants or carriers. 2. 根据权利要求 1所述的含海藻酸钠寡糖的叶面肥组合物, 其特征在于: 所述 氨基酸包含甘氨酸、 丙氨酸、 异亮氨酸、 胱氨酸、 半胱氨酸、 甲硫氨酸、 苏氨酸、 丝 氨酸、 苯丙氨酸、 酪氨酸、 色氨酸、 脯氨酸、 羟脯氨酸、 谷氨酸、 天门冬氨酸中的一 种或二种以上;  The alginate oligosaccharide-containing foliar fertilizer composition according to claim 1, wherein the amino acid comprises glycine, alanine, isoleucine, cystine, cysteine, One or more of methionine, threonine, serine, phenylalanine, tyrosine, tryptophan, valine, hydroxyproline, glutamic acid, aspartic acid; 所述微量元素肥料为能够提供植物微量元素的肥料,如硼肥、钼肥、锰肥、铜肥、 铁肥、 锌肥中的一种或二种以上;  The trace element fertilizer is a fertilizer capable of providing plant trace elements, such as one or more of boron fertilizer, molybdenum fertilizer, manganese fertilizer, copper fertilizer, iron fertilizer and zinc fertilizer; 所述海藻酸钠寡糖为海带中提取的天然多糖碳水化合物, 由 a-L-甘露糖醛酸 (mannuronic acid, M) 与 b-D-古罗糖醛酸 (guluronic acid, G) 依靠 1, 4-糖苷键连接并 由不同 GGGMMM片段组成的共聚物, 分子式为 (C6H706Na)n, 聚合度 2-10, 糖醛酸 组成为 M/G=7:3, 糖醛酸含量 >90%。 The sodium alginate oligosaccharide is a natural polysaccharide carbohydrate extracted from kelp, and is dependent on 1,4-glycoside by aL-mannuronic acid (M) and bD-guluronic acid (G). a copolymer which is bonded by a different GGGMMM fragment and has a molecular formula of (C 6 H 7 0 6 Na)n, a degree of polymerization of 2-10, a uronic acid composition of M/G=7:3, and a uronic acid content of >90. %. 3. 根据权利要求 1所述的含海藻酸钠寡糖的叶面肥组合物, 其特征在于: 所述 主要活性成份氨基酸、 微量元素和海藻酸钠寡糖质量含量分别为 10-400 g/L、 0.2-50 g/L和 0.1-30 g/L, 它们之间的比例为 20:4:5。  The sodium alginate oligosaccharide-containing foliar fertilizer composition according to claim 1, wherein the main active ingredient amino acid, trace element and sodium alginate oligosaccharide have a mass content of 10-400 g/ L, 0.2-50 g/L and 0.1-30 g/L, the ratio between them is 20:4:5. 4. 根据权利要求 1所述的含海藻酸钠寡糖的叶面肥组合物, 其特征在于: 所述溶剂包括但不局限于: 水、 乙醇、 丙酮、 二甲苯、 甲苯、 氯苯、 环己烷、 甲 醇、 二甲基甲酰胺、 吡咯烷酮、 环己酮、 丁醇、 戊醇、 醚类、 酯类、 二甲基亚砜、 月 桂醇、 二氯甲烷、石油馏出物的一种或二种以上的混合物, 叶面肥组合物中溶剂质量 比例为 50-99%;  The sodium alginate oligosaccharide-containing foliar fertilizer composition according to claim 1, wherein the solvent includes but is not limited to: water, ethanol, acetone, xylene, toluene, chlorobenzene, ring One of hexane, methanol, dimethylformamide, pyrrolidone, cyclohexanone, butanol, pentanol, ethers, esters, dimethyl sulfoxide, lauryl alcohol, dichloromethane, petroleum distillate or a mixture of two or more kinds, the solvent mass ratio of the foliar fertilizer composition is 50-99%; 所述助剂为非离子型助剂和离子型助剂,包括但不局限于: 盐酸、硫酸、冰醋酸、 氢氧化钠、 氢氧化钾、 氨水、 脂肪醇聚氧乙烯醚、 烷基酚聚氧乙烯醚、 聚氧乙烯脂肪 酸酯、 烷基芳基聚二醇醚、 烷基磺酸盐、 芳基磺酸盐、 脂肪酸聚二乙醇、 十二烷基苯 磺酸盐、烷基酚聚氧乙烯醚磷酸酯、聚氧乙烯烷基酚甲醛缩合物的一种或二种以上混 合的水溶液, 水溶液中助剂质量浓度 0.1-2%; 叶面肥组合物中助剂水溶液的质量比 例为 50-99%;  The auxiliary agent is a non-ionic auxiliary agent and an ionic auxiliary agent, including but not limited to: hydrochloric acid, sulfuric acid, glacial acetic acid, sodium hydroxide, potassium hydroxide, ammonia water, fatty alcohol polyoxyethylene ether, alkylphenol poly Oxyethylene vinyl ether, polyoxyethylene fatty acid ester, alkyl aryl polyglycol ether, alkyl sulfonate, aryl sulfonate, fatty acid polydiethanol, dodecylbenzene sulfonate, alkyl phenol condensate One or more mixed aqueous solutions of oxyethylene ether phosphate and polyoxyethylene alkylphenol formaldehyde condensate, the concentration of the auxiliary agent in the aqueous solution is 0.1-2%; the mass ratio of the aqueous solution of the auxiliary agent in the foliar fertilizer composition is 50-99%; 所述载体包括木质素磺酸盐、 甲基纤维素、 烷基磺酸盐、 烷基萘磺酸盐、 脂肪醇 聚氧乙烯醚等的一种或者二种以上混合物, 叶面肥组合物中载体质量比例为 0.05-50%。  The carrier comprises one or a mixture of two or more of lignosulfonate, methylcellulose, alkylsulfonate, alkylnaphthalenesulfonate, fatty alcohol polyoxyethylene ether, etc., in a foliar fertilizer composition The carrier mass ratio is 0.05-50%. 5. 根据权利要求 1所述的含海藻酸钠寡糖的叶面肥组合物, 其特征在于: 所述 所述微量元素肥料为硫酸锌、 硝酸锌、 氯化锌、 磷酸锌、 硫酸铜、 硝酸铜、 氯化铜、 磷酸铜、硝酸锰、硫酸锰、 氯化锰、磷酸锰、硫酸钾、硝酸钾、磷酸钾、硫酸二氢钾、 磷酸氢二钾、 氯化钾、 硫酸镁、 硝酸镁、 氯化镁、 硫酸亚铁、 硫酸铁、 硝酸亚铁、 硝 酸铁、 氯化亚铁、 氯化铁、 磷酸亚铁和磷酸铁中的一种或者二种以上混合物, 其于叶 面肥组合物中质量比例为 0.005-20%。 The sodium alginate oligosaccharide-containing foliar fertilizer composition according to claim 1, wherein the trace element fertilizer is zinc sulfate, zinc nitrate, zinc chloride, zinc phosphate, copper sulfate, Copper nitrate, copper chloride, copper phosphate, manganese nitrate, manganese sulfate, manganese chloride, manganese phosphate, potassium sulfate, potassium nitrate, potassium phosphate, potassium dihydrogen sulfate, dipotassium hydrogen phosphate, potassium chloride, magnesium sulfate, nitric acid Magnesium, magnesium chloride, ferrous sulfate, iron sulfate, ferrous nitrate, nitrate One or a mixture of two or more of ferric acid, ferrous chloride, ferric chloride, ferrous phosphate and iron phosphate in a ratio of 0.005 to 20% by mass in the foliar fertilizer composition. 6. 一种权利要求 1所述含海藻酸钠寡糖的叶面肥组合物的在作物增产及抗旱中 的应用。  6. Use of a sodium alginate oligosaccharide-containing foliar fertilizer composition according to claim 1 for crop yield increase and drought resistance. 7. 根据权利要求 6所述的应用, 其特征在于:  7. The application of claim 6 wherein: 该叶面肥组合物可用于经济作物、 粮食作物、 蔬菜、 果树、 经济林木、 花卉或草 坪; 开花结果类植物保花保果率达到 50-99%, 增加植株干旱条件下抗性相关的各种 酶类和次生代谢物的合成 70%-90%。  The foliar fertilizer composition can be used for economic crops, food crops, vegetables, fruit trees, economic trees, flowers or lawns; flowering fruit plants have a flower-preserving rate of 50-99%, and the resistance-related conditions under the drought conditions of the plants are increased. The synthesis of enzymes and secondary metabolites is 70%-90%. 8. 根据权利要求 6所述的应用, 其特征在于:  8. The application according to claim 6, wherein: 所述经济作物包括烟草、 棉花、 油茶、 油菜、 甜菜、 甘蔗、 大豆、 茶树、 花生中 的一种或者二种以上;  The cash crop includes one or more of tobacco, cotton, camellia, rape, sugar beet, sugar cane, soybean, tea tree, and peanut; 所述粮食作物包括小麦、 水稻、 玉米、 高粱中的一种或者二种以上;  The food crop includes one or more of wheat, rice, corn, and sorghum; 所述蔬菜包括白菜、 苦瓜、 丝瓜、 芹菜、 茄子、 西红柿、 冬瓜、 菠菜、 大蒜、 葱、 辣椒、 黄瓜、 菠菜、 大头菜、 马铃薯、 番薯、 萝卜、 胡萝卜、 豌豆、 菜豆、 四季豆、 毛豆中的一种或者二种以上;  The vegetables include cabbage, bitter gourd, loofah, celery, eggplant, tomato, melon, spinach, garlic, onion, pepper, cucumber, spinach, kohlrabi, potato, sweet potato, radish, carrot, pea, kidney bean, green beans, edamame One or more; 所述水果包括芒果、 西瓜、 苹果、 梨、 香蕉、 木瓜、 柑橘、 橙子、 葡萄、 樱桃、 荔枝、 桃、 李、 杏、 草莓、 菠萝、 蓝莓、 猕猴桃、 枣、 柿子、 哈密瓜等, 三叶、 多变 小冠花等、 匍匐马蹄金、 沿阶草、 百里香、 匍匐委陵菜中的一种或者二种以上; 所述经济林木包括: 橡胶、 椰子、 核桃、 板栗、 榛子中的一种或者二种以上; 所述花卉包括下述中的一种或者二种以上: 一、二年生草本花卉:石竹、万寿菊、 金盏菊、 旱金莲、 三色堇、 凤仙花、 紫茉莉、 虞美人、 花菱草、 一串红、 紫罗兰、 牵 牛花、含羞草、鸡冠花、千日红、 夜落金钱、 天蓝绣球、 四季报春; 多年生草本花卉: 文竹、 凤仙、 吊兰、 君子兰、 香子兰、 紫罗兰、 鹤望兰、 文殊兰、 朱顶红、 万年青、 天门冬、 马蹄莲、 百子莲、 西番莲、 仙客来、 大岩桐、 非洲菊、 太阳花、 秋海棠、 垂 花凤梨; 落叶宿根和球根类草花: 百合、 萱草、 牡丹、 菊花、 芍药、 鸢尾、 水仙、 蜀 葵、 大丽花、 唐菖蒲、 小苍兰、 晚香玉、 郁金香、 风信子、 美人蕉; 水生花卉: 荷花、 睡莲、 凤眼莲、 石菖蒲; 蕨类花卉: 肾蕨、 铁线蕨、 鹿角蕨; 兰科花卉: 春兰、 蕙兰、 建兰、 墨兰、 台兰、 寒兰、 石斛、 兔耳兰、 卡特兰、 香港兜兰; 常绿灌木类: 米兰、 栀子、 含笑、 茉莉、 扶桑、 探春、 棕竹、 一品红、 五色梅、 夜丁香、 六月雪、 迎春花、 素馨花、 夹竹桃、 南天竹、 杜鹃花、 大叶黄杨; 落叶灌木类: 月季、 蔷薇、 玫瑰、 腊 梅、 石榴、 迎春、 木槿、 地棠、 黄刺玫、 珍珠梅、 榆叶梅、 紫丁香、 木芙蓉、 无花果、 贴梗海棠、 木本象牙红; 常绿乔木类: 苏铁、 山棕、 蒲葵、 榕树、 冬青、 桂花、、 山 茶、 佛手、 香圆、 金橘、 翠柏、 龙柏、 橡皮树、 广玉兰、 五针松、 金钱松、 罗汉松、 雪松、 白兰花; 落叶乔木类: 桃花、 梅花、 樱花、 玉兰、 紫荆、 海棠花、 垂丝海棠; 常绿藤本类: 龙吐珠、 龟背竹、 叶子花、常春藤、万字茉莉、 非洲凌宵; 落叶藤本类: 凌宵、 藤本、 木香、 金银花、 爬山虎; 仙人掌与多肉植物: 昙花、 芦荟、 景天、 蟹爪 兰、 龙舌兰、 虎皮兰、 虎刺梅、 霸王鞭、 仙人球类、 令箭荷花、 落地生根、 木叶仙人 掌; 中药材类包括: 人参、 三七、 大黄、 川芎、 天麻、 木耳、 木香、 麦冬、 柴胡、 党参、 黄芪、 黄连、 槟榔、 薄荷、 马钱子、 五味子、 五倍子、 牛蒡子、 冬虫草、 肉豆 蔻、 安息香、 延胡索、 两面针、 何首乌、 板蓝根、 刺五加、 罗汉果、 十大功劳; 草坪 草的种类: 细弱剪股颖、 绒毛剪股颖、 匍匐剪股颖、 小糠草, 韧叶紫羊茅、 匍匐紫羊 茅、 羊茅、 细叶茅和高羊茅等, 草地早熟禾、 普通早熟禾、 林地早熟禾和早熟禾,多年 生黑麦草、 洋狗尾草、 梯牧草、 结缕草、 大穗结缕草、 中华结缕草、 马尼拉结缕草、 细叶结缕草、 白颖苔草、 细叶苔、 异穗苔和卵穗苔草等; 白三叶、 红三叶、 多变小冠 花等、 匍匐马蹄金、 沿阶草、 百里香、 匍匐委陵菜。 The fruit includes mango, watermelon, apple, pear, banana, papaya, citrus, orange, grape, cherry, lychee, peach, plum, apricot, strawberry, pineapple, blueberry, kiwi, jujube, persimmon, cantaloupe, etc., trefoil, One or more of a small crown flower, a horseshoe gold, a step grass, a thyme, a potentilla; the economic forest includes: one of rubber, coconut, walnut, chestnut, hazelnut or Two or more; the flower includes one or more of the following: One or two-year-old herbal flowers: Dianthus, Marigold, Calendula, Nasturtium, Pansy, Impatiens, Mirabilis, Corn Poppy , California Poppy, A String of Red, Violet, Morning Glory, Mimosa, Cockscomb, Amaranth, Night Money, Sky Blue Hydrangea, Four Seasons Primula; Perennial Herbal Flowers: Wenzhu, Impatiens, Chlorophyta, Clivia, Fragrant Lan, Violet, Strelitzia, Manjusri, Zhu Dinghong, Evergreen, Asparagus, Calla Lily, Baizilian, Passiflora, Cyclamen, Gloxinia Gerbera, sun flower, begonia, flowering pineapple; deciduous perennial and bulbous grass flowers: lily, valerian, peony, chrysanthemum, peony, iris, narcissus, hollyhock, dahlia, gladiolus, freesia, tuberose, tulip, Hyacinth, Canna; Aquatic flowers: Lotus, water lily, Eichhornia crassipes, Shichangpu; Fern flowers: Kidney fern, Adiantum, Staghorn fern; Orchid flower: Chunlan, Cymbidium, Jianlan, Molan, Tailan, Hanlan, Dendrobium, Rabbit Ears, Cattleya, Hong Kong Paphiopedilum; Evergreen shrubs: Milan, scorpion, smiling, jasmine, hibiscus, spring, brown bamboo, poinsettia, five-color plum, night lilac, June snow, Winter jasmine, frangipani, oleander, southern bamboo, rhododendron, big leaf boxwood; deciduous shrubs: rose, rose, rose, wax, pomegranate, spring, hibiscus, mantle, yellow thorn, pearl plum, cilantro Plum, lilac, wood hibiscus, fig, stalked sea bream, woody ivory red; evergreen arbor: cycad, mountain brown, palmetto, eucalyptus, winter Green, Osmanthus, Camellia, Bergamot, Fragrant, Kumquat, Cypress, Cypress, Rubber Tree, Magnolia, Pine, Pine, Pine, Cedar, White Orchid; Deciduous Trees: Peach Blossom, Plum Blossom, Cherry blossom, magnolia, bauhinia, Begonia, and scorpionfish; evergreen vines: dragon spit, monstera, bougainvillea, ivy, jasmine, African locust; Clover, vine, woody, honeysuckle, ivy; cactus and succulents: scented flowers, aloe vera, sedum, crab claw orchid, agave, tiger skin, tiger thorn plum, tyrannosaurus, prickly pear, lotus flower, rooting , Wood leaf cactus; Chinese medicinal materials include: ginseng, notoginseng, rhubarb, Chuanxiong, Tianma, fungus, woody, Ophiopogon japonicus, Bupleurum, Codonopsis, Astragalus, Coptis, Areca, Mint, Strychnos, Schisandra, Gallic, Burdock, Cordyceps, Nutmeg, Benzoin, Corydalis, Bilateral Needle, Polygonum, Banlangen, Acanthopanax, Mangosteen, Ten Contributions; Types of Lawn Grass: Thin Pruning, Fluffy, 匍匐, 匍匐, Kobresia, T. chinensis, T. chinensis, Fescue, Tall Fescue and Tall Fescue, Kentucky bluegrass, common bluegrass, bluegrass and bluegrass, perennial ryegrass, foxtail, ladder Forage, Zoysia, Big Spike, Chinese Zoysia, Manila Zoysia, Kobresia, White Spear, Fine Leaf Moss, Isochaled moss and egg stalks; white clover, red clover, variegated small crown flower, etc., 匍匐 horseshoe gold, along the grass, thyme, 匍匐 匍匐. 9. 根据权利要求 6所述的应用, 其特征在于:  9. The application of claim 6 wherein: 所述叶面肥组合物稀释 10-2000倍液,于作物花蕾期叶面喷施至叶片完全打湿为 止, 7-10天喷施 1-3次, 连续喷施 3-4次。  The foliar fertilizer composition is diluted 10-2000 times, sprayed on the foliage of the flower bud stage until the leaves are completely wetted, sprayed 1-3 times in 7-10 days, and sprayed 3-4 times continuously.
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