[go: up one dir, main page]

WO2013113822A3 - Ozone scavengers for nucleic acid hybridization experiments - Google Patents

Ozone scavengers for nucleic acid hybridization experiments Download PDF

Info

Publication number
WO2013113822A3
WO2013113822A3 PCT/EP2013/051915 EP2013051915W WO2013113822A3 WO 2013113822 A3 WO2013113822 A3 WO 2013113822A3 EP 2013051915 W EP2013051915 W EP 2013051915W WO 2013113822 A3 WO2013113822 A3 WO 2013113822A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid hybridization
hybridization experiments
ozone scavengers
scavengers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2013/051915
Other languages
French (fr)
Other versions
WO2013113822A2 (en
Inventor
Frank Bergmann
Dieter Heindl
Hans-Peter Josel
John Luckey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Publication of WO2013113822A2 publication Critical patent/WO2013113822A2/en
Publication of WO2013113822A3 publication Critical patent/WO2013113822A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a method, a buffer and a kit for scavenging reactive oxygen species in nucleic acid hybridization experiments.
PCT/EP2013/051915 2012-02-03 2013-01-31 Ozone scavengers for nucleic acid hybridization experiments Ceased WO2013113822A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261594414P 2012-02-03 2012-02-03
US61/594,414 2012-02-03

Publications (2)

Publication Number Publication Date
WO2013113822A2 WO2013113822A2 (en) 2013-08-08
WO2013113822A3 true WO2013113822A3 (en) 2013-10-10

Family

ID=48326245

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2013/051915 Ceased WO2013113822A2 (en) 2012-02-03 2013-01-31 Ozone scavengers for nucleic acid hybridization experiments

Country Status (1)

Country Link
WO (1) WO2013113822A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113186037B (en) * 2021-04-23 2022-12-13 山东博弘基因科技有限公司 Nucleic acid scavenger

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108966A1 (en) * 2003-05-30 2004-12-16 Agilent Technologies Inc. Ligand array assays exhibiting reduced fluorescent dye degradation and compositions for practicing the same
WO2012037394A1 (en) * 2010-09-16 2012-03-22 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004108966A1 (en) * 2003-05-30 2004-12-16 Agilent Technologies Inc. Ligand array assays exhibiting reduced fluorescent dye degradation and compositions for practicing the same
WO2012037394A1 (en) * 2010-09-16 2012-03-22 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"Index of /~astuart/CMB_Lab", 22 September 2009 (2009-09-22), Retrieved from the Internet <URL:http://phobos.ramapo.edu/~astuart/CMB_Lab/> [retrieved on 20130613] *
"Search Results", 13 June 2013 (2013-06-13), Retrieved from the Internet <URL:http://www.nimblegen.com/search.html?q=+CGH%2FLOH+> [retrieved on 20130613] *
A. STUART: "Hybridization of Yeast cDNAs to Microarrays", 22 September 2009 (2009-09-22), XP002698814, Retrieved from the Internet <URL:http://phobos.ramapo.edu/~astuart/CMB_Lab/Genisphere_hybridiz_protocol.doc> [retrieved on 20130613] *
CHAN ET AL.: "Development of a Dye-Protecting Solution for Preventing Fluorescent Dye Degradation on DNA microarrays", 15 March 2004 (2004-03-15), XP002698817, Retrieved from the Internet <URL:http://www.chem.agilent.com/library/posters/public/5989_0924en_ozone_abrf.pdf> [retrieved on 20130613] *
J. PAINTER ET AL.: "Metrics for optimizing CGH/LOH array data quality", NIMBLEGENTECHNICAL NOTE FEB 2012, 1 February 2012 (2012-02-01), XP002698816, Retrieved from the Internet <URL:http://www.nimblegen.com/downloads/support/06650007001_NG_CGH-LOH_OptimizingDataQuality_TN_0212.pdf> [retrieved on 20130613] *
LUCKEY ET AL.: "Ozone effects onNimbleGen dual-colorDNA labeling microarraydata quality", NIMBLEGEN TECHNICAL NOTE JANUARY 2012, 21 January 2012 (2012-01-21), XP002698815, Retrieved from the Internet <URL:http://iucontent.iu.edu.sa/scholars/workflow%20development/Ozone%20effects%20on%20NimbleGen%20dual-color%20DNA%20labeling%20microarray%20data%20quality.pdf> [retrieved on 20130613] *

Also Published As

Publication number Publication date
WO2013113822A2 (en) 2013-08-08

Similar Documents

Publication Publication Date Title
GB201219137D0 (en) Direct nucleic acid amplification kit, reagent and method
SG10201601638QA (en) Nucleic acid detection method, and device and kit for use in same
GB2499921B (en) Biophotonic compositions, kits and methods
WO2017091811A3 (en) Methods for variant detection
ZA201403803B (en) Composition,apparatus,kit and method and uses thereof
EP2890966A4 (en) Method and kit for purifying nucleic acids
HK1211260A1 (en) Method and kit for nucleic acid sequencing
GB201516569D0 (en) Methods, compositions, and kits for nucleic acid analysis
WO2013177429A3 (en) Extreme pcr
SG10201403405RA (en) Method, kit and device for detecting nucleic acid
EP2559442A4 (en) Method for regulating microrna content in organisms and uses thereof
PL2658444T3 (en) Method for providing an efficient biosensor, as well as corresponding biosensor, substrate and insertion kit
WO2012154735A3 (en) Thermostable carbonic anhydrases and methods of use thereof
GB2489187B (en) Compositions, methods and related uses for cleaving modified DNA
EP2729464B8 (en) Cyclopropyl-fused-1,3-thiazepines as bace 1 and/or bace 2 inhibitors
EP2745211B8 (en) Multi-structural, multi-level information formalization and structuring method, and associated apparatus
IN2015KN00073A (en)
EP3020826A4 (en) Whole-genome sequencing method based on dna cloning mixing pool
WO2012025615A3 (en) Process and kit for treating hair
WO2012030136A3 (en) Oligonucleotides for detecting listeria spp. and use thereof
GB201218942D0 (en) Encoder, decoder and method
SG11201509297XA (en) Tree Anchoring Apparatus, Kit and Method
WO2013093627A3 (en) In silico affinity maturation
HUE038608T2 (en) Compositions and methods for nucleic acid hybridization
PL2999798T3 (en) Method for simultaneous detection of bacteria and fungi in a biological preparation by pcr, primers as well as bacteria and fungi detection kit

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 13721270

Country of ref document: EP

Kind code of ref document: A2

122 Ep: pct application non-entry in european phase

Ref document number: 13721270

Country of ref document: EP

Kind code of ref document: A2