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IN2015KN00073A - - Google Patents

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Publication number
IN2015KN00073A
IN2015KN00073A IN73KON2015A IN2015KN00073A IN 2015KN00073 A IN2015KN00073 A IN 2015KN00073A IN 73KON2015 A IN73KON2015 A IN 73KON2015A IN 2015KN00073 A IN2015KN00073 A IN 2015KN00073A
Authority
IN
India
Prior art keywords
relates
present
rna molecule
individual cells
molecule expressed
Prior art date
Application number
Inventor
Yuri Bushkin
Maria Laura Gennaro
Sanjay Tyagi
Richard Pine
Original Assignee
Univ Rutgers
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rutgers filed Critical Univ Rutgers
Publication of IN2015KN00073A publication Critical patent/IN2015KN00073A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6866—Interferon
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention relates to a method for rapidly detecting copies of at least one RNA molecule expressed in individual cells and uses thereof.
IN73KON2015 2012-06-27 2013-06-26 IN2015KN00073A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261665231P 2012-06-27 2012-06-27
US201361784802P 2013-03-14 2013-03-14
PCT/US2013/047774 WO2014004609A2 (en) 2012-06-27 2013-06-26 Rapid assays for t-cell activation by rna measurements using flow cytometry

Publications (1)

Publication Number Publication Date
IN2015KN00073A true IN2015KN00073A (en) 2015-07-31

Family

ID=49784002

Family Applications (1)

Application Number Title Priority Date Filing Date
IN73KON2015 IN2015KN00073A (en) 2012-06-27 2013-06-26

Country Status (12)

Country Link
US (2) US10900072B2 (en)
EP (2) EP3828278A1 (en)
JP (1) JP6464081B2 (en)
KR (1) KR20150036186A (en)
CN (2) CN108624654A (en)
AU (2) AU2013280416B2 (en)
BR (1) BR112014032677A2 (en)
CA (1) CA2877933A1 (en)
IN (1) IN2015KN00073A (en)
RU (1) RU2671707C2 (en)
WO (1) WO2014004609A2 (en)
ZA (1) ZA201500239B (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4361608A3 (en) 2012-02-03 2024-07-24 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
EP3901243A1 (en) 2012-08-03 2021-10-27 California Institute of Technology Multiplexing and quantification in pcr with reduced hardware and requirements
CN106198358B (en) * 2016-06-24 2019-02-15 北京大学 Chromosome sorting method based on "excitation in quartz cup" flow cytometer
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
CN108732082A (en) * 2018-06-07 2018-11-02 北京唯公医疗技术有限公司 cell grouping method
US12203129B2 (en) * 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7457572B2 (en) * 2019-05-27 2024-03-28 住友化学株式会社 Method and device for measuring warpage of optical film
EP4025595A4 (en) * 2019-09-03 2023-11-29 The Regents Of The University Of California METHOD FOR SEQUENCE OF MRNA IN PARALLEL SINGLE CELLS WITH QUANTIFICATION OF INTRACELLULAR PHENOTYPE
US20220381770A1 (en) * 2019-10-29 2022-12-01 Universitaet Heidelberg Artificial antigen presenting molecules and their uses
CN111999235A (en) * 2020-07-03 2020-11-27 青岛农业大学 A method for rapid detection of viable edible fungus protoplasts by flow cytometry
RU2766781C1 (en) * 2021-03-03 2022-03-15 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр кардиологии" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ кардиологии" Минздрава России) Method for detecting vascular endothelium involvement
WO2022271272A1 (en) * 2021-06-22 2022-12-29 Nantcell, Inc. Rapid test for patient cd8 t-cell activation, systems and methods

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US4994369A (en) * 1987-12-15 1991-02-19 The Board Of Trustees Of The Leland Stanford Jr. Univ. T-cell activation related gene
US5866331A (en) 1995-10-20 1999-02-02 University Of Massachusetts Single molecule detection by in situ hybridization
US6610839B1 (en) * 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
DE69723531T3 (en) * 1996-10-01 2012-09-06 Geron Corp. Catalytic subunit of human telomerase
US7514232B2 (en) * 1996-12-06 2009-04-07 Becton, Dickinson And Company Method for detecting T cell response to specific antigens in whole blood
RU2258710C2 (en) 1999-03-09 2005-08-20 Займодженетикс, Инк. New cytokine zalpha 11-ligand
CN102406937A (en) 1999-03-09 2012-04-11 津莫吉尼蒂克斯公司 Novel cytokine zalpha11 ligand
US20040033517A1 (en) 2002-04-19 2004-02-19 Wolfram Ruf Compositions and methods relating to endothelial cell signaling using the protease activated receptor (PAR1)
AU2003258090B2 (en) * 2002-08-08 2010-07-22 Baylor College Of Medicine Isolation and identification of T cells
EP1765988B1 (en) * 2004-05-27 2017-09-20 The Trustees of The University of Pennsylvania Novel artificial antigen presenting cells and uses therefor
DE102005020754B3 (en) 2005-05-02 2007-01-11 Altana Pharma Ag Cellular assay method for the identification of PKCtheta inhibitors
US20090081688A1 (en) * 2005-06-20 2009-03-26 Advanced Cell Diagnostics Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations
CN101495650B (en) 2005-06-20 2015-02-04 领先细胞医疗诊断有限公司 Method for detection of nucleic acids in single cells and identification of rare cells in heterogeneous large cell populations
ES2330013T3 (en) 2005-09-05 2009-12-03 Immatics Biotechnologies Gmbh PEPTIDES ASSOCIATED WITH TUMORS UNITED TO MOLECULES OF THE HUMAN LEUCOCITE ANTIGEN (HLA) CLASS I OR II AND RELATED CANCER VACCINES.
WO2008069940A2 (en) * 2006-12-06 2008-06-12 Albert Einstein College Of Medicine Of Yeshiva University Multi-microrna methods and compositions
WO2009003493A2 (en) 2007-07-03 2009-01-08 Dako Denmark A/S Mhc multimers, methods for their generation, labeling and use
JP2010004750A (en) 2008-06-24 2010-01-14 Institute For Rheumatic Diseases Co Ltd Method for diagnosing onset or onset possibility of tissue disorder by autoimmune disease, and utilization thereof
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ES2363545B2 (en) * 2010-01-26 2012-04-18 Universidade De Santiago De Compostela METHOD FOR IDENTIFYING AND PURIFYING HUMAN NATURAL REGULATOR T CELLS (NTREG).
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JP5344007B2 (en) * 2011-06-30 2013-11-20 株式会社デンソー Near field communication device

Also Published As

Publication number Publication date
AU2017228677B2 (en) 2019-02-14
US20210139965A1 (en) 2021-05-13
EP2867374A4 (en) 2016-05-11
AU2013280416A1 (en) 2015-02-05
BR112014032677A2 (en) 2019-01-15
RU2015102447A (en) 2016-08-20
WO2014004609A2 (en) 2014-01-03
AU2013280416B2 (en) 2017-06-15
JP2015522284A (en) 2015-08-06
EP2867374B1 (en) 2020-10-28
WO2014004609A3 (en) 2014-02-27
US11767551B2 (en) 2023-09-26
CN108624654A (en) 2018-10-09
KR20150036186A (en) 2015-04-07
US10900072B2 (en) 2021-01-26
CN104662170A (en) 2015-05-27
EP2867374A2 (en) 2015-05-06
ZA201500239B (en) 2015-12-23
US20160201122A1 (en) 2016-07-14
RU2671707C2 (en) 2018-11-06
JP6464081B2 (en) 2019-02-06
AU2017228677A1 (en) 2017-10-05
EP3828278A1 (en) 2021-06-02
CA2877933A1 (en) 2014-01-03

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