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WO2013012269A3 - 전능성 줄기세포로부터 희소돌기아교 전구세포의 제조 방법 - Google Patents

전능성 줄기세포로부터 희소돌기아교 전구세포의 제조 방법 Download PDF

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Publication number
WO2013012269A3
WO2013012269A3 PCT/KR2012/005775 KR2012005775W WO2013012269A3 WO 2013012269 A3 WO2013012269 A3 WO 2013012269A3 KR 2012005775 W KR2012005775 W KR 2012005775W WO 2013012269 A3 WO2013012269 A3 WO 2013012269A3
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cell
pluripotent stem
precursor cell
producing
oligodendrocyte precursor
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French (fr)
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WO2013012269A2 (ko
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김동욱
김대성
임보영
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Industry Academic Cooperation Foundation of Yonsei University
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Industry Academic Cooperation Foundation of Yonsei University
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Abstract

본 발명은 전능성 줄기세포로부터 희소돌기아교 전구세포의 제조 방법 및 방법에 의해 제조된 신경전구세포에 관한 것으로서, 보다 상세하게는, (a) 전능성 줄기세포를 신경 로제트(neural rosette)로 분화시켜 신경전구세포를 포함하는 세포 파퓰레이션을 얻는 단계; (b) 상기 세포 파퓰레이션에 A2B5에 특이적으로 결합하는 항체를 접촉시키는 단계; (c) 상기 A2B5에 대한 항체가 결합된 A2B5 양성 세포를 분리하는 단계; 및 (d) 상기 A2B5 양성 세포를 희소돌기아교 전구세포로 분화하는 단계에 관한 것이다. 본 발명에 따르면, 기존의 방법과 비교하여 효율적이고 안정된 희소돌기아교 전구세포의 제조 방법을 확립하고 표준화 할 수 있으며, 분화과정을 추적하기에 적합한 표지인자제공 할 수 있다. 또한, 분화 과정 중에 생길 수 있는 변수에 의한 분화 수율이 일정하게 유지되지 못하는 단점을 극복할 수 있으며, 분화과정을 단축하여 보다 고순도 제조방법을 제공할 수 있다.
PCT/KR2012/005775 2011-07-20 2012-07-19 전능성 줄기세포로부터 희소돌기아교 전구세포의 제조 방법 Ceased WO2013012269A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2011-0071895 2011-07-20
KR1020110071895A KR101330649B1 (ko) 2011-07-20 2011-07-20 전능성 줄기세포로부터 희소돌기아교전구세포의 제조 방법

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WO2013012269A2 WO2013012269A2 (ko) 2013-01-24
WO2013012269A3 true WO2013012269A3 (ko) 2013-04-11

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10801010B2 (en) 2014-03-07 2020-10-13 Unist (Ulsan National Institute Of Science And Technology) Composition for inducing direct transdifferentiation into oligodendrocyte progenitor cells from somatic cells and use thereof
SG11201909501TA (en) * 2017-04-13 2019-11-28 Univ Leland Stanford Junior Personalized 3d neural culture system for generating human oligodendrocytes and studying myelination in vitro
CN108624560B (zh) * 2018-06-01 2022-04-08 南京艾尔普再生医学科技有限公司 一种分化培养基及少突胶质前体细胞的制备方法
KR20210027290A (ko) * 2018-06-06 2021-03-10 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 신경 줄기 세포 조성물 및 신경변성 장애를 치료하는 방법
CN114540301B (zh) * 2020-11-20 2024-02-27 上海纽仁生物医药科技有限公司 少突胶质前体细胞亚群及其用途

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KR100941036B1 (ko) 2007-10-05 2010-02-05 고려대학교 산학협력단 인간 배아줄기세포로부터 척수신경계 희소돌기 아교세포생산을 위한 삼단계 분화기법

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ABRANCHES, E. ET AL.: "Neural differentiation of embryonic stem cells in vitro: a road map to neurogenesis in the embryo", PLOS ONE, vol. 4, no. 7, 21 July 2009 (2009-07-21), pages E6286 *
ELKABETZ, Y. ET AL.: "Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage", GENES & DEVELOPMENT, vol. 22, no. 2, 15 January 2008 (2008-01-15), pages 152 - 165 *
NUNES, MC. ET AL.: "Identification and isolation of multipotential neural progenitor cells from the subcortical white matter of the adult human brain", NATURE MEDICINE, vol. 9, no. 4, April 2003 (2003-04-01), pages 439 - 447 *
OGAWA, S-I. ET AL.: "Immunopanning selection of A2B5-positive cells increased the differentiation efficiency of induced pluripotent stem cells into oligodendrocytes", NEUROSCIENCE LETTERS, vol. 489, no. 2, 4 February 2011 (2011-02-04), pages 79 - 83 *
ZHANG, S-C. ET AL.: "In vitro differentiation of transplantable neural precursors from human embryonic stem cells", NATURE BIOTECHNOLOGY, vol. 19, no. 12, 2001, pages 1129 - 1133 *

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KR101330649B1 (ko) 2013-11-15
WO2013012269A2 (ko) 2013-01-24

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