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WO2013067611A1 - Procédé enzymatique à froid pour la production d'éthanol - Google Patents

Procédé enzymatique à froid pour la production d'éthanol Download PDF

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Publication number
WO2013067611A1
WO2013067611A1 PCT/BR2012/000433 BR2012000433W WO2013067611A1 WO 2013067611 A1 WO2013067611 A1 WO 2013067611A1 BR 2012000433 W BR2012000433 W BR 2012000433W WO 2013067611 A1 WO2013067611 A1 WO 2013067611A1
Authority
WO
WIPO (PCT)
Prior art keywords
enzymatic process
ethanol
production
heated
ethanol production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/BR2012/000433
Other languages
English (en)
Portuguese (pt)
Inventor
Eduardo CAUDURO MALLMANN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of WO2013067611A1 publication Critical patent/WO2013067611A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the present invention describes a cold enzymatic process for ethanol production. More specifically it comprises a process that utilizes starch hydrolyzing enzyme for simultaneous fermentation and cold saccharification.
  • Biofuels have shown great potential for larger scale substitution of petroleum derivatives.
  • Sugar cane, corn, sugar beet, oilseeds such as soybean, castor oil, palm oil, in addition to plant biomass such as cellulose, hemiceiulose and lignin are interesting sources for the generation of this type of fuel.
  • Ethanol ⁇ CH 3 CH 2 OH also called ethyl alcohol and, in ordinary language, simply alcohol, is an Organic substance obtained from the fermentation of sugars, hydration of ethylene or reduction to acetoide.
  • ethanol is widely used as a fuel for explosion engines, thus constituting a growing market for renewable fuel and the establishment of a basic chemical industry, sustained by the use of biomass of agricultural and renewable origin.
  • the ethanol production process is divided into three major stages: a) preparation of the raw material, pre-cooking to gelatinization, hydrolysis / saccharification with the addition of ⁇ -amylase and glucoamylase (saccharifying); fermentation and distillation.
  • Enzymatic hydrolysis and fermentation may occur separately or simultaneously. If it occurs separately, the advantage is the possibility of optimizing the enzymatic hydrolysis and fermentation temperatures, where in enzymatic hydrolysis the cellulose is broken down into cellobiose, which is subsequently converted into two glucose molecules by ⁇ -giucosidase. . However, endogiucanase activity is inhibited by cellobiose and ⁇ -glucosidase activity by glucose.
  • Simultaneous saccharification and fermentation is a way to prevent enzymatic inhibition, as as glucose is being formed it is also being consumed for ethanol production, leading to greater cellulose conversion.
  • an intermediate temperature condition is required for the enzymes and the yeast added once the optimum temperature for saccharification is about 55 to 30 C and S C for fermentation.
  • the invention provides a cold enzymatic process for production of ethanol with hydrophysication of granular starch without the need for conventional gelatinization and liquefaction processes.
  • the invention provides a cold enzymatic process for ethanol production that eliminates the need for activators such as calcium and caustic soda to enhance enzymatic activity.
  • the invention provides a cold enzymatic process for ethanol production that provides higher ethanol production per kilogram of biomass.
  • the invention provides a cold enzymatic process for ethanol production that reduces the energy requirement for heating the wheels.
  • the invention provides a cold enzymatic process for ethanol production that eliminates the essential vitamin and amino acid losses in the simultaneous cold fermentation and saccharification process.
  • the invention provides a cold enzymatic process for ethanol production that provides lower glycerine production when compared to hot processes, with a reduction of about 20%.
  • Figure 1 presents the graph comparing the pH of starch and the pH of ethanol stored in dornas
  • the cold enzymatic process for ethanol production comprises in a first step the disintegration of the raw material, selected from grains, roots or tubers.
  • the disintegration is preferably carried out with a 0.8mm sieve, followed by hydration of the raw material at a rate of 04 liters per kilogram for grains and 1 liter per kilogram for roots and tubers.
  • the raw material is washed and peeled, followed by comminution.
  • the disintegrated and hydrated raw material is stored in drums for the addition of 0.02 to 0.08 wt% biomass and yeast between 0.095 and 0.0105 for the breakdown of molecules. of sucrose.
  • the stored biomass pH in the drums should be between 3.8 and 4.5, as shown in figure 1, and the temperature between 31 ° C and 33 ° C, with constant stirring to keep the solids in suspension for up to 75 hours.

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé enzymatique à froid pour la production d'éthanol avec fermentation et saccharification à froid de manière simultanée, faisant intervenir des enzymes hydrolysantes d'amidon et de levures, dans des conditions spécifiques de température, d'agitation et de pH, selon la biomasse utilisée.
PCT/BR2012/000433 2011-11-07 2012-11-06 Procédé enzymatique à froid pour la production d'éthanol Ceased WO2013067611A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
BRPI1106666-0 2011-11-07
BRPI1106666A BRPI1106666A2 (pt) 2011-11-07 2011-11-07 processo enzimático a frio para produção de etanol

Publications (1)

Publication Number Publication Date
WO2013067611A1 true WO2013067611A1 (fr) 2013-05-16

Family

ID=48288394

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BR2012/000433 Ceased WO2013067611A1 (fr) 2011-11-07 2012-11-06 Procédé enzymatique à froid pour la production d'éthanol

Country Status (3)

Country Link
BR (1) BRPI1106666A2 (fr)
UY (1) UY34438A (fr)
WO (1) WO2013067611A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070281344A1 (en) * 2006-06-06 2007-12-06 Lantero Oreste J Process for conversion of granular starch to ethanol
WO2008021050A1 (fr) * 2006-08-11 2008-02-21 Danisco Us, Inc., Genencor Division Amylases de céréales naturelles dans des associations d'enzymes pour l'hydrolyse d'amidon granulé
WO2009114403A1 (fr) * 2008-03-11 2009-09-17 Danisco Us Inc., Genencor Division Utilisation de rhizopus amilases dans l’hydrolyse d’amidon en granules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070281344A1 (en) * 2006-06-06 2007-12-06 Lantero Oreste J Process for conversion of granular starch to ethanol
WO2008021050A1 (fr) * 2006-08-11 2008-02-21 Danisco Us, Inc., Genencor Division Amylases de céréales naturelles dans des associations d'enzymes pour l'hydrolyse d'amidon granulé
WO2009114403A1 (fr) * 2008-03-11 2009-09-17 Danisco Us Inc., Genencor Division Utilisation de rhizopus amilases dans l’hydrolyse d’amidon en granules

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CARDONA, C.A. ET AL.: "Production of bioethanol from sugarcane bagasse: Status and perspectives", BIORESOURCE TECHNOLOGY, vol. 101, 2010, pages 4754 - 4766, XP026986209 *
ROBERTSON, G.H ET AL.: "Native or raw starch digestion: A key step in energy efficient biorefining of grain", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 54, 2006, pages 353 - 365, XP002525742 *
SANCHEZ, O.J. ET AL.: "Trends in biotechnological production of fuel ethanol from different foodstocks", BIORESOURCE TECHNOLOGY, vol. 99, 2008, pages 5270 - 5295, XP022647362 *
SANTOS, J.R.A. ET AL.: "Seleção de linhagem industrial de Saccharomyces cerevisiae com potencial desempenho para a produção de etanol em condições adversas de temperatura e agitação.", REVISTA BRASILEIRA DE PRODUTOS AGROINDUSTRIAIS, vol. 12, no. 1, 2010, pages 75 - 80 *

Also Published As

Publication number Publication date
UY34438A (es) 2013-06-28
BRPI1106666A2 (pt) 2016-01-19

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