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WO2013046818A1 - Dispositif d'essai, accepteur d'objet d'essai et système d'essai - Google Patents

Dispositif d'essai, accepteur d'objet d'essai et système d'essai Download PDF

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Publication number
WO2013046818A1
WO2013046818A1 PCT/JP2012/065336 JP2012065336W WO2013046818A1 WO 2013046818 A1 WO2013046818 A1 WO 2013046818A1 JP 2012065336 W JP2012065336 W JP 2012065336W WO 2013046818 A1 WO2013046818 A1 WO 2013046818A1
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WO
WIPO (PCT)
Prior art keywords
receiver
holder
holders
liquid
inspection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2012/065336
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English (en)
Japanese (ja)
Inventor
由美子 大鹿
千里 吉村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brother Industries Ltd
Original Assignee
Brother Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brother Industries Ltd filed Critical Brother Industries Ltd
Publication of WO2013046818A1 publication Critical patent/WO2013046818A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/04—Investigating sedimentation of particle suspensions
    • G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
    • G01N2015/045—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates by optical analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges

Definitions

  • the present invention relates to a test object receiver for performing chemical, medical, and biological tests, a test apparatus that applies centrifugal force by rotating the test target receptor, and a test system composed of these. About.
  • Patent Document 1 an inspection apparatus for inspecting biological substances, chemical substances, and the like by centrifuging an object to be inspected called a microchip or an inspection chip has been proposed (for example, see Patent Document 1).
  • an analysis tool including a liquid inlet provided in the central portion and a plurality of flow paths extending from the liquid inlet to the peripheral portion, and an inspection is performed by horizontally rotating the analytical tool.
  • An apparatus is disclosed. In such an inspection apparatus, the inspection object moves to the measurement site provided on the downstream side of the flow path by the centrifugal treatment of the inspection object receptacle. After the execution of the centrifugal process, the measurement site of the test object receiver is irradiated with light from the light source, and the light transmitted through the test object is received by the sensor to obtain the test result.
  • the inspection object is optically measured by transmitting light in the vertical direction with respect to the measurement part of the inspection object receptacle.
  • the light source and sensor it is necessary to arrange the light source and sensor so that the light source and sensor are not applied to the rotating unit. Therefore, the distance between the light source and the sensor is increased, and there is a possibility that the apparatus member interferes in the optical path.
  • the present invention has been made in order to solve the above-described problem. Even when optical measurement is performed from a direction different from the vertical direction with respect to the measurement portion of the inspection target receptacle, the optical path connecting the light source and the sensor is not affected. It is an object of the present invention to provide an inspection apparatus, an inspection object receiver, and an inspection system that can suppress interference of other inspection object receivers.
  • the inspection apparatus of the present invention includes an injection port into which a liquid that is an inspection object is injected, a flow path extending in a planar direction in which the liquid injected from the injection port is movable, and a downstream side of the flow path
  • the inspection object receiver formed with the storage part for storing the liquid is rotated around a first axis separated from the inspection object receiver, and the liquid is caused to flow in the flow path by centrifugal force.
  • a plurality of receiver holders each holding the inspection target receiver in a posture in which an extending direction of the flow path and a virtual plane including the first axis are parallel to each other;
  • a first drive unit that rotates the plurality of receiver holders in one direction around the first axis in a state where the inspection target receiver is held by at least one of the plurality of receiver holders;
  • a second axis extending in a direction perpendicular to the virtual plane
  • a plurality of receiver holders that can be rotated in both directions, and the plurality of receiver holders rotated by a predetermined amount by the first driver and then held by the receiver holder.
  • a measuring unit that measures the liquid by transmitting light from the direction intersecting the centrifugal direction to the storage unit, and the measuring unit is rotated by the receiving holder.
  • the second drive unit includes a target holder that is the receiver holder that holds the inspection target receiver to be measured when the liquid is measured by the measurement unit, the light source and the sensor. Position where the connecting optical path passes through the reservoir Rotate, and, different from other of said receptacle holder and said target holder is rotated to a position away from the optical path connecting the light source and the sensor.
  • the box-shaped receiver holder holding the inspection target receiver is rotated about the first axis, and the extending direction of the flow path and the virtual plane including the first axis are parallel to each other. Centrifugal force is applied to the test object receptacle held in a posture that makes After the receiver holder is rotated by a predetermined amount, the liquid is measured by transmitting light to the storage portion.
  • the measuring unit has a light source and a sensor outside the range in which the receiver holder is rotated.
  • the target holder is rotated to a position where the optical path passes through the storage section, and the other receiver holder is rotated to a position away from the optical path. Thereby, it is possible to prevent the optical path from being interfered with by another receiver holder at the time of measuring the liquid, and it is possible to accurately measure the liquid existing in the storage portion of the inspection target receiver held by the target holder.
  • the second drive unit changes the rotation angle of the target holder to the first angle when the liquid is measured by the measurement unit, and the other holder holder different from the target holder Among them, the interference holder positioned in the optical path may be changed to a second angle having a predetermined angular difference with respect to the first angle. In this case, it is possible to prevent the optical path from being interfered by the interference holder at the time of liquid measurement only by rotating the interference holder at an angle different from that of the target holder.
  • the plurality of receiver holders are box-shaped bodies having the same shape having an internal space into which the inspection target receiver can be inserted and removed, and the receiver holder includes an extending direction of the flow path.
  • a pair of openings provided opposite to both side surfaces of the receiver holder that are substantially parallel, wherein the reservoir is in the centrifugal direction in a state in which the test subject receiver is inserted into the receiver holder.
  • the first angle is a rotation angle through which the optical path passes through the measurement port of the target holder when the liquid is measured by the measurement unit
  • the second angle The angle may be a rotation angle through which the optical path passes through a position separated from the interference holder when the liquid is measured by the measurement unit.
  • the plurality of receiver holders are box-shaped bodies having the same shape, and have a measurement port that exposes the reservoir of the test target receiver inserted therein.
  • the target holder is changed to an angle at which the optical path passes through the measurement port, and the interference holder is changed to a second angle away from the optical path. Therefore, it is possible to accurately measure the liquid existing in the storage part of the inspection target receptacle held by the target holder by the optical path passing through the measurement port.
  • the inspection object receiver of the present invention is the inspection object receiver used in the inspection device, and the storage unit includes the first holder and the first holder that hold the inspection object receiver. Regardless of which of the two angles is rotated, it may be a part where the outflow of the liquid that has flowed into the reservoir is restricted. In this case, even when the plurality of receiver holders are rotated at either the first angle or the second angle, the inspection target receiver is restricted from flowing out of the liquid from the reservoir. Therefore, it is possible to continuously measure the inspection object receivers respectively held by the plurality of object holders.
  • the plurality of receiver holders are arranged in a plurality of points symmetrically about the first axis, and the second drive unit is arranged symmetrically about the first axis.
  • Each set of the holder holders is rotated in conjunction with each other, and the measurement units are arranged in a set that is point-symmetric about the first axis, and the set of measurement units is point-symmetric.
  • Each set of the receiver holders arranged may be measured as the target holder. In this case, it is possible to simultaneously measure each of the inspection object receivers held by the set of receiver holders with a set of measuring units.
  • the inspection system of the present invention is configured to rotate the inspection object receiver around a first object axis that is separated from the inspection object receiver, and the inspection object receiver that stores the liquid that is the inspection object,
  • An inspection system comprising: an inspection device that applies centrifugal force to a target receiver and measures the liquid, wherein the inspection target receiver is injected from the injection port and the injection port to which the liquid is injected A flow path extending in a planar direction in which the liquid can move, and a storage section in which the liquid moved to the downstream side of the flow path is stored, and the inspection apparatus includes the extending direction of the flow path and the A plurality of receiver holders each holding the inspection object receiver in a posture parallel to a virtual plane including the first axis; and at least one of the plurality of receiver holders, the inspection object receiver In a state where the plurality of receiver holders are The plurality of receiver holders are rotated in both directions around a first drive section that rotates in one direction around one axis and a second axis that extends in a direction ortho
  • the storage unit is centrifuged for each of the inspection target receivers held by the receiver holder.
  • a measuring unit that measures the liquid by transmitting light from a direction that intersects the direction, and the measuring unit is configured to measure the inspection target receptacle to be measured from outside the range in which the receiver holder is rotated.
  • the box-shaped receiver holder holding the inspection target receiver is rotated about the first axis, and the extending direction of the flow path and the virtual plane including the first axis are parallel to each other. Centrifugal force is applied to the test object receptacle held in a posture that makes After the receiver holder is rotated by a predetermined amount, the liquid is measured by transmitting light to the storage portion.
  • the measuring unit has a light source and a sensor outside the range in which the receiver holder is rotated.
  • the target holder is rotated to a position where the optical path passes through the storage section, and the other receiver holder is rotated to a position away from the optical path. Thereby, it is possible to prevent the optical path from being interfered by other receiver holders when measuring the liquid, and to accurately measure the liquid present in the storage portion of the target holder.
  • FIG. 2 It is a top view of the test
  • 2 is a perspective view of a receiver holder 100.
  • FIG. It is a front view of the test object receiver. It is a front view of the receiving body holder 100 in which the test object receiving body 2 was accommodated.
  • 3 is a block diagram showing an electrical configuration of a control device 90.
  • FIG. It is a figure which shows the state change of the test object receiver 2 in the receiver holder 100 revolved in the steady state. It is a figure which shows the state change of the test object receiver 2 in the receiver holder 100 revolved in the displacement state. It is a figure which shows the state change of the test object receiver 2 after the centrifugation process in the receiver holder 100 of a steady state. It is a figure which shows the state change of the test object receiver 2 after the centrifugation process in the receiver holder 100 of a displacement state.
  • the inspection apparatus 1 can apply a centrifugal force to the inspection object receiver 2 by rotation about a vertical axis that is a first axis separated from the inspection object receiver 2.
  • the inspection apparatus 1 can switch the centrifugal direction, which is the direction of the centrifugal force applied to the inspection target receptacle 2, by rotating the inspection target receptacle 2 around the horizontal axis that is the second axis. is there.
  • FIG. 1 shows a state in which the top plate of the upper housing 30 is removed, and FIGS. 2 to 5 do not show the upper housing 30 and the upper plate 61.
  • FIGS. 2 to 5 show the left side view of the inspection apparatus 1 shown in FIGS. 2 to 5 shows a longitudinal section at the center in the left-right direction of the inspection apparatus 1.
  • the inspection apparatus 1 includes an upper housing 30, a lower housing 31, a turntable 33, a plurality of receiver holders 100, an angle changing mechanism 34, a control device 90, and the like.
  • the lower casing 31 is supported on the installation surface of the inspection apparatus 1 by the leg portions 50 at the four corners.
  • the lower housing 31 is provided with a drive mechanism for rotating the turntable 33 around a vertical axis.
  • the turntable 33 is provided on the upper surface side of the lower housing 31.
  • the turntable 33 is a disk-shaped rotating body on which the plurality of receiver holders 100 are held upward.
  • the receiver holder 100 is a box-shaped body that holds the inspection target receiver 2 inside.
  • the angle changing mechanism 34 is provided on the turntable 33.
  • the angle changing mechanism 34 is a drive mechanism that rotates the plurality of receiver holders 100 around the horizontal axis.
  • the upper housing 30 is fixed on the upper side of the lower housing 31.
  • the upper housing 30 is provided with a measuring unit 7 for optically measuring the liquid contained in the inspection object receiver 2.
  • the control device 90 is a controller that controls centrifugal processing, measurement processing, and the like of the inspection device 1.
  • the lower housing 31 has a box-shaped frame structure in which frame members are combined.
  • an upper plate 32 that is a rectangular plate material in plan view is provided.
  • a turntable 33 is rotatably provided above the upper plate 32.
  • a middle frame member 52 extending horizontally in the front-rear direction, which is the left-right direction shown in FIGS.
  • a spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed near the rear in the lower housing 31.
  • a shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed.
  • a vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31 in plan view.
  • the main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31.
  • the upper end portion of the main shaft 57 is connected to the center portion of the turntable 33 in plan view.
  • a support member 53 that is a holding metal fitting through which the main shaft 57 passes is provided directly below the upper plate 32.
  • the support member 53 is fixed to the middle frame member 52 by a pair of frames 54.
  • the main shaft 57 is rotatably held by the support member 53.
  • a pulley 38 is fixed below the support member 53 on the main shaft 57.
  • a belt 39 is stretched over the pulleys 37 and 38.
  • a guide rail 56 extending vertically from the bottom surface of the lower housing 31 to the lower surface of the upper plate 32 is provided near the front in the lower housing 31.
  • the T-shaped plate 48 which is a T-shaped plate-shaped connection fitting, is movable in the vertical direction within the lower housing 31 along the guide rail 56.
  • a laterally long groove portion 80 is formed on the left side of the T-shaped plate 48 which is the back side of the paper surface shown in FIGS.
  • the above-described main shaft 57 is a hollow cylindrical body.
  • the inner shaft 40 is a vertical shaft that can move in the vertical direction inside the main shaft 57.
  • An upper end portion of the inner shaft 40 passes through the main shaft 57 and is connected to a round rack gear 43 described later.
  • a bearing 55 fixed to the middle frame member 52 is provided below the main shaft 57.
  • a bearing 41 fixed to the rear end portion of the T-shaped plate 48 is provided below the bearing 55.
  • a bearing (not shown) is provided inside the bearing 41, and a lower end portion of the inner shaft 40 is inserted therein.
  • the intermediate portion and the lower end portion of the inner shaft 40 are rotatably held by bearings 55 and 41, respectively.
  • a stepping motor 51 for moving the T-shaped plate 48 up and down is fixed by a fixing tool (not shown).
  • the shaft 58 of the stepping motor 51 protrudes toward the right side that is the front side of the paper shown in FIGS. 2 to 5, and a disc-shaped cam plate 59 is fixed to the tip.
  • a cylindrical projection 70 is provided on the right surface of the cam plate 59. Since the tip of the protrusion 70 is inserted into the groove 80 described above, the protrusion 70 can slide in the groove 80.
  • the stepping motor 51 rotates the shaft 58, the projection 70 moves up and down in conjunction with the rotation of the cam plate 59.
  • the T-shaped plate 48 moves up and down along the guide rail 56 in conjunction with the protrusion 70 inserted in the groove 80.
  • the angle changing mechanism 34 includes a first plate 60 and a second plate 63 which are a plurality of L-shaped plates 6 which are L-shaped plate-shaped connecting fittings fixed to the upper surface of the turntable 33.
  • Each L-shaped plate 6 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and an upper end portion thereof extends outward in the radial direction of the turntable 33.
  • a round rack gear 43 fixed to the inner shaft 40 is provided between the L-shaped plates 6.
  • the round rack gear 43 is a vertically long metal rod-like member, and a gear is engraved on the entire circumference in the vertical direction.
  • first plates 60 and four second plates 63 that are eight L-shaped plates 6 are alternately arranged so as to extend radially from the rotation center of the turntable 33 in a plan view.
  • the eight L-shaped plates 6 are equally arranged so that the adjacent first plate 60 and second plate 63 form 45 degrees in plan view. That is, the eight L-shaped plates 6 include two sets of first plates 60 arranged symmetrically with respect to the rotation center of the turntable 33 and two sets of second plates 63 arranged symmetrically with respect to the points. Including.
  • the second plate 63 extends above the first plate 60.
  • a horizontal shaft 46 of the gear 45 is rotatably supported at the distal end side in the extending direction of each L-shaped plate 6. Since the shaft 46 is fixed to the receiver holder 100, the receiver holder 100 also rotates about the shaft 46 in conjunction with the rotation of the gear 45.
  • Each L-shaped plate 6 is provided with a pinion gear 44 that is supported so as to be rotatable about a horizontal axis on the rotation center side of the turntable 33 with respect to the gear 45.
  • each second plate 63 is provided with a gear 47 supported above the pinion gear 44 and the gear 45 so as to be rotatable about a horizontal axis.
  • the pinion gear 44 meshes with the gear 47 and the round rack gear 43, and the gear 47 meshes with the gear 45.
  • eight receiver holders 100A to 100H provided on the eight L-shaped plates 6 are arranged at intervals of 45 degrees around the round rack gear 43 in plan view.
  • four receiver holders 100A, 100C, 100E, and 100G provided on the four first plates 60 are arranged at intervals of 90 degrees in a plan view.
  • the acceptor holders 100A and 100E form a pair arranged opposite to each other with the revolution center interposed therebetween, and the acceptor holders 100C and 100G constitute a pair arranged to face each other across the revolution center.
  • four receiver holders 100B, 100D, 100F, and 100H provided on the four second plates 63 are arranged at intervals of 90 degrees in a plan view.
  • the acceptor holders 100B and 100F form a pair arranged opposite to each other with the revolution center interposed therebetween, and the acceptor holders 100D and 100H constitute a pair arranged oppositely across the revolution center.
  • a cylindrical guide member 42 is provided at the upper end of the round rack gear 43.
  • a plurality of frames 62 are erected in the vicinity of the rotation center of the turntable 33. Upper ends of the plurality of frames 62 are fixed to a plate-like upper plate 61, respectively.
  • the guide member 42 is held so as to be slidable in the vertical direction while being inserted into an opening formed in the central portion of the upper plate 61.
  • each first plate 60 the pinion gear 44 and the gear 45 are driven to rotate in conjunction with the vertical movement of the round rack gear 43, and the receiver holders 100A to 100H rotate about the shaft 46. That is, the four receiver holders 100A, 100C, 100E, and 100G supported by the four first plates 60 rotate in the same direction and angle around the horizontal axis.
  • the pinion gear 44, the gear 47, and the gear 45 are driven to rotate in conjunction with the vertical movement of the round rack gear 43, and as a result, the receiver holder 100 rotates about the shaft 46. That is, the four receiver holders 100B, 100D, 100F, and 100H supported by the four second plates 63 rotate around the horizontal axis in the same direction and angle.
  • the chip holder 100 of the first plate 60 and the chip holder 100 of the second plate 63 rotate in opposite directions around the horizontal axis.
  • the receiver holders 100A to 100H rotate around the main shaft 57 as the vertical axis, which is the first axis, to receive the inspection object. Centrifugal force is applied to the body 2.
  • the rotation around the vertical axis of the receiver holder 100 is referred to as “revolution”.
  • the receiver holders 100A to 100H rotate around the shaft 46 as the horizontal axis, which is the second axis, so that the inspection object receiver 2 is rotated.
  • the acting centrifugal direction changes relatively.
  • the rotation around the horizontal axis of the receiver holder 100 is referred to as “rotation”.
  • each of the receiver holders 100A to 100H the extending direction of channels 24 and 25 described later and a vertical plane that is a virtual plane including the main shaft 57 are parallel to each other regardless of the change in angle due to rotation and revolution.
  • Each test subject receptacle 2 is held inside in a posture to be made.
  • the receiving holders 100A, 100C, 100E, and 100G of the first plate 60 are in a steady state to be described later. Further, in FIG. 1 and FIG. 2, the receiver holders 100A and 100E are located at the measurement positions described later. However, in FIG. 3, for convenience of explanation, the inspection apparatus 1 shown in FIGS. 1 and 2 is revolved 45 degrees counterclockwise in plan view without changing the rotation angle of each of the holders 100A to 100H. ing.
  • the receiving holders 100B, 100D, 100F, and 100H of the second plate 63 are in a steady state to be described later. Furthermore, in FIG. 4, the receiver holders 100B and 100F are located at the measurement positions described later.
  • the inspection apparatus 1 shown in FIG. 4 is revolved 45 degrees counterclockwise in a plan view without changing the rotation angle of each of the receiver holders 100A to 100H. 2 to 5, a pair of receiver holders 100 arranged before and after the round rack gear 43 are indicated by solid lines, and the other receiver holders 100 located on both sides of the pair of receiver holders 100 are indicated by phantom lines. Is shown.
  • the round rack gear 43 is also lowered to the uppermost end of the movable range.
  • the receiver holders 100B, 100D, 100F, and 100H of the second plate 63 are in a steady state as shown in FIG.
  • the receiving holders 100A, 100C, 100E, and 100G of the first plate 60 are in a displaced state as shown in FIG. That is, the autorotatable range that is the angular width that each receiver holder 100 can rotate is a range from the steady state (0 degrees) to the displaced state (90 degrees).
  • the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper side of the upper plate 32. More specifically, the upper housing 30 is provided on the outer peripheral side of the turntable 33 in plan view, and has an opposing wall 81 that surrounds a range in which the receiver holder 100 is rotated.
  • a pair of measuring units 7 are provided on both the front and rear sides inside the upper housing 30.
  • Each measuring unit 7 measures the liquid in the inspection target receptacle 2 by transmitting light extending in a direction intersecting with the extending direction of the below-described flow paths 24 and 25 to the inspection target receptacle 2 to be measured. To do. Specifically, each measurement unit 7 transmits light extending in the horizontal direction perpendicular to the extending direction of the flow paths 24 and 25 to the storage unit 23 to be measured.
  • Each measuring unit 7 includes a light source 71 that emits measurement light and an optical sensor 72 that detects the measurement light emitted from the light source 71.
  • the light source 71 is provided in the right front part of the inspection apparatus 1 which is the lower right part shown in FIG. 1, while the optical sensor 72 is provided in the left front part of the inspection apparatus 1 which is the upper right part shown in FIG. ing.
  • the light source 71 is provided in the left rear part of the inspection apparatus 1 which is the upper left part shown in FIG. 1, while the optical sensor 72 is provided in the right rear part of the inspection apparatus 1 which is the lower left part shown in FIG. It has been. That is, the light source 71 and the optical sensor 72 of each measuring unit 7 are respectively disposed on the left and right sides of the turntable 33 that are the upper and lower sides shown in FIG. 1 outside the rotation range of the receiver holder 100.
  • the measurement light emitted from the light source 71 to the left side of the inspection apparatus 1 is received by the optical sensor 72.
  • measurement light emitted from the light source 71 to the right side of the inspection apparatus 1 is received by the optical sensor 72.
  • the height position of the optical path connecting the light source 71 and the optical sensor 72 is equal to the height position of the measurement port 120 described later with reference to the receiver holder 100 in a steady state.
  • the facing wall 81 is formed with an exposure port (not shown) for exposing the light source 71 and the optical sensor 72 of each measurement unit 7 to the outside of the upper housing 30.
  • the receiver 2 becomes the inspection target receiver 2 to be measured. More specifically, the position where the two measurement lights extending in the left-right direction of the inspection apparatus 1 are orthogonal to the front and rear surfaces of the pair of receiver holders 100 in plan view is the measurement position. In the inspection object receiver 2 to be measured, the measurement light passes through a measurement port 120 described later.
  • each of the receiver holders 100A to 100H revolves by the rotation of the turntable 33, and a centrifugal force is applied to the inspection target receiver 2.
  • the respective holder holders 100A to 100H are rotated by the vertical movement of the round rack gear 43, whereby the direction of the centrifugal force applied to the inspection object receiver 2 is changed.
  • the liquid in the test object receptacle 2 is stirred.
  • the holders 100A and 100E are moved to the measurement position as shown in FIG. Further, as shown in FIG. 2, the round rack gear 43 is lowered to the lowest end of the movable range, and the receiver holders 100A, 100C, 100E, 100G of the first plate 60 are in a steady state. In this state, two optical paths that pass through the measurement ports 120 of the receiver holders 100A and 100E are formed, and the liquid contained in the inspection target receivers 2 held by the receiver holders 100A and 100E is inspected. .
  • the receiving holders 100B, 100D, 100F, and 100H of the second plate 63 are in a displaced state. That is, both the adjacent holder holders 100B and 100H that easily interfere with the optical path formed on the receiver holder 100A are in a displaced state. At the same time, both the adjacent holder holders 100D and 100F that are likely to interfere with the optical path formed on the receiver holder 100E are in a displaced state. As a result, the receiver holders 100B, 100D, 100F, and 100H are all retracted above the measurement ports 120 of the receiver holders 100A and 100E, thereby preventing the possibility of interference with the optical path.
  • the turntable 33 is rotated 45 degrees, whereby the receiver holders 100B and 100F are moved to the measurement positions as shown in FIG. Further, the round rack gear 43 rises to the uppermost end of the movable range, and the receiver holders 100B, 100D, 100F, and 100H of the second plate 63 are in a steady state. In this state, two optical paths that pass through the measurement ports 120 of the receiver holders 100B and 100F are formed, and the liquid contained in the inspection target receivers 2 held by the receiver holders 100B and 100F is inspected. .
  • the receiving holders 100A, 100C, 100E, and 100G of the first plate 60 are in a displaced state. That is, both the adjacent holder holders 100C and 100A that are likely to interfere with the optical path formed on the receiver holder 100B are in a displaced state. At the same time, the adjacent holder holders 100G and 100E that are likely to interfere with the optical path formed on the receiver holder 100F are both in a displaced state. As a result, the receiver holders 100A, 100C, 100E, and 100G are all retracted above the measurement ports 120 of the receiver holders 100A and 100E, so that the possibility of interference with the optical path is prevented.
  • the turntable 33 rotates 45 degrees, the round rack gear 43 descends to the lowest end of the movable range, and each of the two optical paths formed on the receiver holders 100C and 100G.
  • the inspection object receiver 2 is measured.
  • the turntable 33 rotates 45 degrees, the round rack gear 43 rises to the uppermost end of the movable range, and each of the two optical paths formed on the receiver holders 100D and 100H.
  • the inspection object receiver 2 is measured.
  • the other receiver holder 100 adjacent to the receiver holder 100 in which the optical path is formed is in a displaced state, so that interference with the optical path by the other receiver holder 100 is prevented.
  • the upper, lower, lower right, upper left, lower left, and upper left in FIG. 6 are the upper, lower, left, right, front, and rear of the receiver holder 100, respectively.
  • the pair of receiver holders 100 are disposed above the turntable 33 so as to face each other with the rotation center of the turntable 33 interposed therebetween.
  • Each receiver holder 100 has a bottom wall 101, a right wall 102, a left wall 103, a front wall 104, and a rear wall 105, which are walls surrounding other than the upper side, and an opening 107 is formed on the upper side.
  • the opening 107 is formed in a rectangular shape in plan view corresponding to the inspection object receptacle 2 described later. Therefore, the test subject receiver 2 can be inserted into and removed from the internal space of the receiver holder 100 through the opening 107.
  • the upper end of the left wall 103 is formed with a recess 109 into which the locking claw 114 is fitted with the lid 106 closed.
  • the lever member 113 is biased in a direction in which the locking claw 114 is locked to the recess 109 by a spring not shown.
  • the receiver holder 100 has a measurement port 120 that is an opening for measuring the liquid stored in the inspection target receiver 2.
  • the measurement port 120 is a pair of openings 121 and 122 arranged to face the front wall 104 and the rear wall 105 that are both side surfaces of the receiver holder 100.
  • the measurement port 120 is provided at a position that is downstream in the centrifugal direction in the receiver holder 100 and downstream in the gravity direction in a steady state. More specifically, the opening 121 is provided in the lower left portion of the front wall 104, which is the lower right portion shown in FIG. 6, and the opening 122 is provided in the lower left portion of the rear wall 105, which is the lower right portion shown in FIG. It has been.
  • the openings 121 and 122 are formed in the same square shape and communicate with each other in the front-rear direction through the internal space of the receiver holder 100.
  • the inspection object receptacle 2 is formed in a square shape in a front view, and mainly includes a transparent synthetic resin plate material 20 having a predetermined thickness. On the front surface of the plate member 20, storage portions 21, 22, and 23 that are three depressions and flow paths 24 and 25 that are two groove portions are formed.
  • the storage unit 21 is formed in the upper right part of the test target receptacle 2 which is the upper left part shown in FIG. 7 and stores the specimen injected into the test target receiver 2.
  • the storage part 22 is formed below the storage part 21 of the test object receptacle 2 and stores the reagent injected into the test object receiver 2.
  • the storage part 23 is formed in the lower left part of the test object receptacle 2 which is the lower right part shown in FIG. 7, and stores the liquid in which the specimen and the reagent are stirred by the centrifugal process.
  • the flow path 24 extends from the storage unit 21 to the storage unit 23, and the injected specimen can move according to the centrifugal force.
  • the flow path 25 extends from the storage unit 22 to the storage unit 23, and the injected reagent can move according to the centrifugal force.
  • the reservoir 23 is a part where the outflow of the inflowed liquid is restricted regardless of the rotation angle of the receiver holder 100 holding the inspection target receiver 2 between 0 degrees and 90 degrees.
  • the storage part 23 is formed in a rhombus shape in a front view, and the flow paths 24 and 25 are connected to the upper end part.
  • a backflow prevention wall 231 that extends downward to the right so as to protrude into the storage portion 23 is provided immediately below the connection portion of the flow paths 24 and 25 in the storage portion 23. The backflow prevention wall 231 restricts the liquid flowing into the storage unit 23 from flowing back into the flow paths 24 and 25.
  • the vertical distance H1 from the lower end portion of the storage portion 23 to the backflow prevention wall 231 is injected into the inspection target receptacle 2 in a state where the inspection target receptacle 2 is held by the steady state holder holder 100. It is a depth that can contain the mixed liquid of the specimen and the reagent to be measured.
  • the distance H2 in the left-right direction from the left end portion of the reservoir 23 to the backflow prevention wall 231 is injected into the inspection target receptacle 2 in a state where the inspection target receptacle 2 is held by the receiving holder 100 in a displaced state. It is a depth that can accommodate the mixed liquid of the specimen and the reagent.
  • the front surface of the plate member 20 is sealed by a cover member 200 made of a transparent synthetic resin thin plate.
  • the cover member 200 is formed with an injection port 201 that is an opening for injecting a specimen into the storage unit 21 and an injection port 202 that is an opening for injecting a reagent into the storage unit 22.
  • the sealing patch 210 is attached to the injection port 201 and the injection port 202, respectively. Thereby, the specimen and the reagent stored in the storage units 21 and 22 are prevented from flowing out from the injection ports 201 and 202 to the outside of the test target receptacle 2.
  • the control device 90 includes a CPU 91 that controls the main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for inputting instructions to the control device 90, a hard disk device (HDD) 95 for storing various data and programs, a display 96 for displaying various information, and the like.
  • a CPU 91 that controls the main control of the inspection device 1
  • a RAM 92 that temporarily stores various data
  • a ROM 93 that stores a control program.
  • an operation unit 94 for inputting instructions to the control device 90
  • HDD hard disk device
  • display 96 for displaying various information, and the like.
  • a revolution controller 97 controls the revolution of the receiver holders 100A to 100H by driving the spindle motor 35 to rotate.
  • the rotation controller 98 controls the rotation of the receiver holders 100A to 100H by driving the stepping motor 51 to rotate.
  • the measurement controller 99 performs optical measurement of the inspection target by performing light emission control of the light source 71 and detection control of the optical sensor 72 for each of the pair of measurement units 7.
  • the user drops a specimen and a reagent respectively to the reservoirs 21 and 22 of the test object receptacle 2 before performing the test, and seals the inlets 201 and 202 with the sealing patch 210, respectively. Thereafter, the user inserts the inspection object receiver 2 into the receiver holders 100A to 100H, and inputs a process start command from the operation unit 94. Thereby, the CPU 91 executes an inspection process as exemplified below based on the control program stored in the ROM 93. In FIGS. 10 and 11, the centrifugal direction acting on the test object receptacle 2 is indicated by an arrow.
  • the rotation controller 98 lowers the round rack gear 43 to the lowest end of the movable range. Accordingly, the receiver holders 100A, 100C, 100E, and 100G that are the receiver holders 100 of the first plate 60 are in the steady state shown in FIG. 2, and the receiver holders 100B and 100D that are the receiver holders 100 of the second plate 63 are obtained. , 100F, 100H are in the displacement state shown in FIG.
  • the revolution controller 97 rotates the turntable 33 to start a centrifugal process for revolving the receiver holders 100A to 100H at a predetermined rotational speed.
  • the specimen in the reservoir 21 flows into the reservoir 23 via the flow path 24 by centrifugal force in the receiver holder 100 of the first plate 60, as shown in FIG.
  • the reagent in the reservoir 22 flows into the reservoir 23 via the flow path 25 by centrifugal force and mixes with the specimen.
  • the acceptor holder 100 of the second plate 63 is in a displaced state, the specimen and the reagent in the reservoirs 21 and 22 do not move, or even if they move, the amount is very small.
  • the rotation controller 98 raises the round rack gear 43 to the uppermost end of the movable range.
  • the receiver holder 100 of the second plate 63 rotates by -90 degrees to the steady state shown in FIG. 4, and the receiver holder 100 of the first plate 60 rotates by 90 degrees to the displacement state shown in FIG. .
  • the mixed liquid of the specimen and the reagent is agitated in the storage unit 23 by the change in the centrifugal direction.
  • the receiver holder 100 of the second plate 63 as shown in FIG. 10
  • the specimen and reagent in the reservoirs 21 and 22 move through the channels 24 and 25 by centrifugal force and are mixed in the reservoir 23.
  • the revolution controller 97 controls the revolution position of the turntable 33 so that the holder holders 100A, 100E of the pair of first plates 60 stop at the measurement position.
  • the mixed liquid is stored within the range of the distance H1 in the storage unit 23.
  • the liquid mixture is stored within the range of the distance H2 in the storage unit.
  • the backflow prevention wall 231 prevents the mixed liquid from flowing out from the storage portion 23.
  • the measurement controller 99 executes a measurement process of the liquid present in the inspection target receptacle 2 after the centrifugal process. Specifically, in each measurement unit 7, measurement light is emitted from the light source 71 toward the optical sensor 72. Since each measurement light passes through each measurement port 120 of the pair of receiver holders 100A and 100E existing at the measurement position, an optical path that passes through the storage unit 23 of the inspection target receiver 2 exposed from each measurement port 120 is obtained. Form each one. Since each measurement light is attenuated when passing through the liquid mixture in the reservoir 23, a test result of the liquid mixture is obtained based on the amount of light received by each optical sensor 72. That is, it is possible to simultaneously measure each liquid mixture present in each inspection object receiver 2 held in one set of receptor holders 100 by one measurement process.
  • another receiver holder 100 adjacent to the pair of receiver holders 100 present at the measurement position is controlled to be in a displaced state so as not to interfere with the measurement light.
  • the receiver holders 100A and 100E of the first plate 60 existing at the measurement position are in a steady state, whereas the receiver holders 100B, 100D and 100F of the second plate 63 positioned on both sides thereof.
  • 100H is the displacement state.
  • the respective reservoirs 23 of the receiver holders 100A and 100E are exposed in the left-right direction without being covered with the other receiver holders 100B, 100D, 100F, and 100H as shown in FIG.
  • an optical path that passes through the storage portion 23 of the receiver holder 100 existing at the measurement position is formed without being interfered with other receiver holders 100, and an accurate measurement result can be obtained.
  • the receiver holder 100 can be separated from the optical path with a relatively short movement distance.
  • an optical path connecting the light source 71 and the optical sensor 72 can be formed at a position overlapping the other receiver holder 100 in plan view. Therefore, not only the freedom degree of arrangement
  • the turntable 33 revolves 45 degrees, and the round rack gear 43 rises to the uppermost end of the movable range.
  • the receiver holder 100 of the second plate 63 is in the steady state shown in FIG. 4, the receiver holder 100 of the first plate 60 is in the displaced state shown in FIG. 100B and 100F move to the measurement position.
  • the mixed liquid is stored within the range of the distance H2 in the storage unit.
  • the mixed liquid is stored within the range of the distance H1 in the storage unit 23. In this state, the measurement process is executed as described above.
  • the turntable 33 revolves 45 degrees, and the round rack gear 43 descends to the lowest end of the movable range.
  • the receiver holder 100 of the first plate 60 is in the steady state shown in FIG. 2, and the receiver holder 100 of the second plate 63 is in the displaced state shown in FIG.
  • the other holder holders 100C and 100G of the first plate 60 move to the measurement position. In this state, the measurement process is executed as described above.
  • the turntable 33 revolves 45 degrees and the round rack gear 43 rises to the uppermost end of the movable range.
  • the receiver holder 100 of the second plate 63 is in the steady state shown in FIG. 4, the receiver holder 100 of the first plate 60 is in the displaced state shown in FIG. 5, and the receiver holder 100D of the other set of second plates 63 is reached. , 100H moves to the measurement position. In this state, the measurement process is executed as described above. By the above inspection method, the measurement of the mixed liquid is executed for all the inspection object receivers 2 held in the eight receiver holders 100A to 100H of the inspection apparatus 1.
  • the box-shaped receiver holder 100 that holds the inspection object receiver 2 is revolved around the main shaft 57 and the flow paths 24 and 25 are extended. Centrifugal force is applied to the test object receptacle 2 held in a posture in which the direction and the virtual plane including the main shaft 57 are parallel to each other.
  • the measuring unit 7 has a light source 71 and an optical sensor 72 outside the range in which the receiver holder 100 is revolved.
  • the receiving holder 100 to be measured is rotated to a position where the optical path passes through the storage portion 23 of the receiving object 2 to be inspected, and the other receiving holder 100 is rotated to a position away from the optical path. .
  • the other receiver holder 100 positioned in the optical path is rotated at an angle different from that of the receiver holder 100 to be measured, and the optical path is prevented from being interfered by the other receiver holder 100 during the measurement of the mixed liquid. it can. More specifically, at the time of measuring the mixed liquid, the receiving holder 100 to be measured is changed to an angle (0 degrees) through which the optical path passes through the measurement port 120, and the other receiving holders 100 are separated from the optical path (90 degrees). ). Therefore, it is possible to accurately measure the liquid mixture existing in the storage unit 23 of the inspection object receiver 2 held in the measurement object receiver holder 100 by the optical path passing through the measurement port 120.
  • the outflow of the mixed liquid from the storage unit 23 is restricted. Therefore, it is possible to continuously measure the inspection object receivers 2 respectively held by the plurality of receiver holders 100. Further, the inspection object receivers 2 held by the set of receptor holders 100 are simultaneously measured by the set of measurement units 7, thereby efficiently inspecting using a large number of inspection object receivers 2. be able to.
  • the present invention is not limited to the above embodiment, and it goes without saying that various modifications are possible.
  • the structures of the inspection apparatus 1, the inspection object receiver 2, and the reception holder 100, the number of the reception holders 100, the rotation angle, the centrifugal direction, and the like are merely examples, and may be determined according to the measurement conditions. .
  • the eight receiving holders 100 are provided in order to simultaneously inspect a maximum of eight inspection target receivers, but the number of the receiving holders 100 can be appropriately changed.
  • the plurality of receiver holders 100 are simultaneously rotated via one movable member (round rack gear 43), but the structure for rotating the receiver holder 100 can be changed as appropriate. It is. For example, a structure in which a plurality of sets of receiver holders 100 are independently rotated by a group may be employed, or a structure in which the plurality of receiver holders 100 are all independently rotated may be employed.
  • the rotation angle of the receiver holder 100 is 0 degree to 90 degrees.
  • the inspection apparatus 1 may rotate the receiver holder 100 in another angle range (for example, 0 degrees to 180 degrees).
  • the rotation angle of the other receiver holder 100 that obstructs the optical path is not limited to 90 degrees, and the other receiver holder 100 that obstructs the optical path may be rotated at an angle (for example, 45 degrees) away from the optical path.

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Abstract

La présente invention porte sur un dispositif d'essai qui, même lorsqu'une position de mesure de l'accepteur d'objet d'essai est mesurée optiquement à partir d'une direction autre que la direction verticale, est apte à supprimer une interférence par d'autres accepteurs d'objet d'essai avec le trajet optique reliant une source lumineuse et un capteur ; l'invention porte également sur un accepteur d'objet d'essai et un système d'essai. Dans ce dispositif d'essai (1), un dispositif de maintien d'accepteur en forme de boîte (100) qui maintient un accepteur d'objet d'essai (2) est mis en rotation autour d'un axe vertical et une force centrifuge est appliquée à l'accepteur d'objet d'essai (2) maintenu dans une orientation parallèle au plan vertical. Après que le dispositif de maintien d'accepteur (100) a tourné d'une quantité prescrite, une lumière est émise dans une unité de réservoir de l'accepteur d'objet d'essai (2) et le liquide est mesuré. Une unité de mesure (7) a une source lumineuse (71) et un capteur optique (72) à l'extérieur de la zone où le dispositif de maintien d'accepteur (100) est mis en rotation. Lors de la mesure du liquide, le dispositif de maintien d'accepteur (100) à mesurer est amené à tourner jusqu'à une position à laquelle le trajet optique passe à travers l'unité de réservoir et les autres dispositifs de maintien d'accepteur (100) sont amenés à tourner jusqu'à une position distante du trajet optique.
PCT/JP2012/065336 2011-09-30 2012-06-15 Dispositif d'essai, accepteur d'objet d'essai et système d'essai Ceased WO2013046818A1 (fr)

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JP2011-218627 2011-09-30
JP2011218627A JP5585561B2 (ja) 2011-09-30 2011-09-30 検査装置、検査システム、およびコンピュータプログラム

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009058406A (ja) * 2007-08-31 2009-03-19 Nsk Ltd 遠心力付与装置及び検体液分析装置
WO2009066737A1 (fr) * 2007-11-20 2009-05-28 Toray Industries, Inc. Cristaux d'alimentation en liquide et procédé d'analyse
JP2010066195A (ja) * 2008-09-12 2010-03-25 Seiko Epson Corp 生体試料反応用チップ、生体試料反応用チップに反応液を充填する遠心装置、および生体試料反応用チップに反応液を充填する方法
WO2010050208A1 (fr) * 2008-10-28 2010-05-06 藤倉化成株式会社 Dispositif de trajet d'écoulement de liquide et son procédé de fabrication

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009058406A (ja) * 2007-08-31 2009-03-19 Nsk Ltd 遠心力付与装置及び検体液分析装置
WO2009066737A1 (fr) * 2007-11-20 2009-05-28 Toray Industries, Inc. Cristaux d'alimentation en liquide et procédé d'analyse
JP2010066195A (ja) * 2008-09-12 2010-03-25 Seiko Epson Corp 生体試料反応用チップ、生体試料反応用チップに反応液を充填する遠心装置、および生体試料反応用チップに反応液を充填する方法
WO2010050208A1 (fr) * 2008-10-28 2010-05-06 藤倉化成株式会社 Dispositif de trajet d'écoulement de liquide et son procédé de fabrication

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