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WO2015152230A1 - Puce de détection - Google Patents

Puce de détection Download PDF

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Publication number
WO2015152230A1
WO2015152230A1 PCT/JP2015/060104 JP2015060104W WO2015152230A1 WO 2015152230 A1 WO2015152230 A1 WO 2015152230A1 JP 2015060104 W JP2015060104 W JP 2015060104W WO 2015152230 A1 WO2015152230 A1 WO 2015152230A1
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
wall surface
holding
unit
injection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2015/060104
Other languages
English (en)
Japanese (ja)
Inventor
由美子 大鹿
千里 吉村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brother Industries Ltd
Original Assignee
Brother Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brother Industries Ltd filed Critical Brother Industries Ltd
Publication of WO2015152230A1 publication Critical patent/WO2015152230A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/07—Centrifugal type cuvettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0409—Moving fluids with specific forces or mechanical means specific forces centrifugal forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00495—Centrifuges
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides

Definitions

  • This disclosure relates to a test chip in which a sample or a reagent is quantified in a quantification unit.
  • a test chip in which a sample or a reagent is quantified in a quantification unit is known.
  • a centrifugal force is applied to the microchip in the first direction, and the whole blood sample in the sample tube is taken out.
  • a centrifugal force is applied in a second direction orthogonal to the first direction, the whole blood sample is introduced into the separation unit, and the centrifugal separation is performed to separate the plasma component and the blood cell component.
  • centrifugal force in the first direction is applied, and the plasma component in the upper layer is removed. At this time, the removed plasma component is accommodated in the first region.
  • centrifugal force in the second direction is applied, the blood cell component liquid level in the separation part is adjusted, and the removed plasma component is moved to the second region.
  • a centrifugal force in a third direction opposite to the first direction is applied, and the liquid reagent R0 in the first liquid reagent holding unit is introduced into the liquid reagent measuring unit and weighed.
  • the liquid reagent R1 in the second liquid reagent holding unit and the liquid reagent R2 in the third liquid reagent holding unit move to the first liquid reagent storage unit and the second liquid reagent storage unit, respectively.
  • the blood cell component in the separation unit is introduced into the blood cell measurement unit and measured.
  • a centrifugal force in the second direction is applied, and the measured blood cell component and the liquid reagent R0 are mixed in the first mixing unit to obtain a mixed solution. Due to this centrifugal force, the liquid reagent R2 in the second liquid reagent container is measured by the liquid reagent metering unit. Subsequently, centrifugal forces in the third direction, the second direction, the first direction, and the second direction are sequentially applied to sufficiently mix the liquid mixture. By applying the centrifugal force in the first direction, the liquid reagent R1 in the first liquid reagent storage unit is measured by the liquid reagent measuring unit. Further, the measured liquid reagent R1 moves to the second mixing unit by the centrifugal force in the second direction.
  • the centrifugal force in the first direction is then applied in the fourth direction, and the supernatant of the mixed solution in the first mixing unit is introduced into the mixed solution measuring unit, and weighing is performed. Is done.
  • the measured mixed liquid and the liquid reagent R1 are mixed in the second mixed liquid.
  • centrifugal forces in the first direction and the second direction are sequentially applied to sufficiently mix the liquid mixture. In a state where the centrifugal force in the second direction is applied, the measured liquid reagent R2 is located in the third region.
  • a centrifugal force in the third direction is applied, the mixed solution and the liquid reagent R2 are mixed in the detection unit, and further, a centrifugal force in the second direction is applied and mixing is sufficiently performed. Finally, a centrifugal force in the third direction is applied, the mixed solution is accommodated in the detection unit, light is irradiated on the detection unit, and optical measurement is performed such that the intensity of the transmitted light is measured.
  • the direction of the centrifugal force before the switching and the direction of the centrifugal force after the switching are different in the period before and after the switching. Centrifugal force is applied, and there is a possibility that the whole blood sample or the liquid reagent does not flow into the measuring unit. Therefore, there is a problem that the quantitative accuracy is lowered.
  • the purpose of the present disclosure is to provide a test chip that improves quantitative accuracy.
  • a test chip includes an injection part into which a specimen or a reagent is injected, a quantification part in which the specimen or the reagent is quantified, and a flow path between the injection part and the quantification part.
  • a second guide part connected to a second end part which is the other end part of the quantification part, and the specimen or the reagent overflowing from the quantification part, and the injection part, and the quantification part,
  • the plurality of holding portions are concave portions or bent wall surfaces that open in a crossing direction that intersects the opening direction.
  • the path from the injection part to the quantification part becomes longer, and the injected specimen or Even with a large amount of reagent, it is possible to hold a sample injected by a plurality of holding units. Therefore, it is possible to reduce the possibility that the sample or reagent flows into the first guide unit or the second guide unit without entering the quantification unit. Therefore, the quantitative accuracy of the specimen or reagent can be improved.
  • the plurality of holding portions include a first holding portion that is a concave portion that opens in the intersecting direction, and a second holding portion that is the bent wall surface that is bent toward the quantitative portion, and the second holding portion is the second holding portion.
  • the first tip portion on the holding portion side may be closer to the injection portion in the crossing direction than the second tip portion on the first holding portion side of the second holding portion.
  • the specimen or reagent flows from the first holding unit to the second holding unit in the flow path between the injection unit and the quantification unit. Moreover, since the first tip portion on the second holding portion side of the first holding portion is closer to the injection portion in the crossing direction than the second tip portion on the first holding portion side of the second holding portion, The specimen or reagent that has flowed out can flow into the second holding part and reduce the possibility of flowing into other parts.
  • the inspection chip may include a third holding portion that is a recess that opens in the crossing direction from between the first tip portion and the second tip portion.
  • the sample or reagent overflowing from the first holding unit can be received by the third holding unit. Therefore, when the specimen or reagent flows from the injection part into the first holding part, the specimen or reagent overflowing from the first holding part can be prevented from flowing into the second holding part.
  • the third holding portion may include a first region extending in a direction opposite to the intersecting direction and a second region extending in a direction opposite to the opening direction.
  • the injection portion includes a first wall portion between the injection portion and the holding portion, and the first holding portion includes a first wall surface that is a wall surface facing the second holding portion side, and forms the injection portion.
  • the first virtual plane passing through the tip of the first wall portion is orthogonal to the first virtual plane when the volume of the specimen or the reagent to be injected into the injection section is a predetermined injection amount.
  • the first imaginary line extending from the tip portion in the outflow direction, which is a direction to perform, may intersect the first wall surface.
  • the sample or reagent when the sample or reagent starts to flow out from the tip of the first wall portion of the injection portion flows out in the first imaginary line direction and hits the first wall surface of the first holding portion. It is possible to reduce the possibility that the specimen or reagent that has flowed out of the section suddenly enters the second holding section or the quantitative section.
  • the first wall portion may be formed at a distance from the first tip portion.
  • the end portion on the first holding portion side of the tip portion of the first wall portion is formed with a gap from the end portion on the first wall portion side of the first wall surface.
  • the sample or reagent overflowing from the first holding part flows from the gap toward the second holding part. Therefore, it is possible to reduce the possibility that the specimen or reagent overflowing from the first holding part flows back to the injection part. Therefore, it is possible to prevent the quantitative amount from being reduced below the specified value, and improve the quantitative accuracy.
  • the inspection chip includes a second wall surface on the second holding portion side in the first guide portion, a third wall surface connected to the second end portion in the second guide portion, and the second wall surface in the first guide portion.
  • the first wall surface angle which is an acute angle formed with the first wall surface and the angle on the first wall portion side, is an acute angle formed with the intersecting direction and the fifth wall surface, and is opposite to the third holding portion.
  • the fifth wall surface angle is equal to or less than the fifth wall surface angle, and the fifth wall surface angle is a second imaginary line that is a tangent line between the second end portion and a tip portion of the fourth wall surface toward the second wall surface, It is an acute angle formed with the intersecting direction and smaller than the fourth wall surface angle that is the angle on the fourth wall surface side. Good.
  • the sample or reagent flowing out from the first holding unit does not remain in the second holding unit when the reagent is injected into the quantifying unit. Can flow into.
  • the fifth wall surface angle is smaller than the fourth wall surface angle, when the sample or reagent is injected from the second holding unit into the quantification unit, the possibility that the sample or reagent gets over the tip of the fourth wall surface can be reduced. Therefore, it is possible to reduce the risk that the sample or reagent is less than the prescribed amount to be quantified, and improve the quantification accuracy.
  • the second holding portion includes a sixth wall surface connected to the fifth wall surface at a bending point and extending from the bending point toward the second tip portion, and the wall surface forming the first holding portion and the first tip portion
  • the third imaginary line may intersect the sixth wall surface.
  • the second holding unit when injecting the sample or reagent from the first holding unit to the second holding unit, the second holding unit is taken along the third imaginary line drawn so that the sample or reagent contacts the first tip. Since it flows in the sixth wall surface direction, the possibility of flowing into the third holding portion can be reduced.
  • the angle on the second holding portion side may be an obtuse angle.
  • FIG. 7 is a state transition diagram of the test chip 2 continued from FIG. 6.
  • FIG. 8 is a state transition diagram of the test chip 2 continued from FIG. 7.
  • FIG. 9 is a state transition diagram of the test chip 2 continued from FIG. 8. It is the elements on larger scale of the front of the test
  • FIG. 1 shows a plane of the inspection apparatus 1 constituting the inspection system 3 and functional blocks inside the control apparatus 90.
  • the inspection system 3 of the present embodiment includes an inspection chip 2 that can store a sample and a reagent that are liquids, and an inspection apparatus 1 that performs an inspection using the inspection chip 2.
  • the inspection device 1 rotates the inspection chip 2 around the vertical axis A ⁇ b> 1 separated from the inspection chip 2, centrifugal force acts on the inspection chip 2.
  • the inspection apparatus 1 rotates the inspection chip 2 around the horizontal axis A2
  • the centrifugal direction which is the direction of the centrifugal force acting on the inspection chip 2 is switched.
  • the inspection system 3 and the inspection apparatus 1 of the present embodiment have a known structure as described in JP 2012-78107 A, and therefore, in the following description, an outline of the structure of the inspection apparatus 1 will be described. To do.
  • FIG. 1 shows a state where the top plate of the upper housing 30 of the inspection apparatus 1 is removed.
  • the inspection apparatus 1 includes an upper housing 30, a lower housing 31, an upper plate 32, a turntable 33, an angle changing mechanism 34, and a control device 90.
  • the turntable 33 is a disk rotatably provided on the upper side of an upper plate 32 described later.
  • the inspection chip 2 is held above the turntable 33.
  • the angle changing mechanism 34 is a drive mechanism provided on the turntable 33.
  • the angle changing mechanism 34 rotates the inspection chip 2 around the horizontal axis A2.
  • the upper housing 30 is fixed to an upper plate 32 described later, and a measurement unit 7 that performs optical measurement on the inspection chip 2 is provided inside.
  • the control device 90 is a controller that controls various processes of the inspection device 1.
  • the schematic structure of the lower housing 31 will be described.
  • the lower housing 31 has a box-shaped frame structure in which frame members are combined.
  • An upper plate 32 that is a rectangular plate material is provided on the upper surface of the lower housing 31.
  • a drive mechanism that rotates the turntable 33 around the vertical axis A1 is provided in the lower housing 31 as follows.
  • a spindle motor 35 that supplies a driving force for rotating the turntable 33 is installed on the left side of the lower housing 31.
  • a shaft 36 of the main shaft motor 35 protrudes upward, and a pulley 37 is fixed.
  • a vertical main shaft 57 extending upward from the inside of the lower housing 31 is provided at the center of the lower housing 31.
  • the main shaft 57 passes through the upper plate 32 and protrudes above the lower housing 31.
  • the upper end portion of the main shaft 57 is connected to the center portion of the turntable 33.
  • the main shaft 57 is rotatably held by a support member (not shown) provided immediately below the upper plate 32.
  • a pulley 38 is fixed to the main shaft 57 below the support member.
  • a belt 39 is stretched over the pulley 37 and the pulley 38.
  • a guide rail (not shown) extending in the vertical direction inside the lower housing 31 is provided on the right side in the lower housing 31.
  • a T-shaped plate (not shown) is movable in the vertical direction in the lower housing 31 along the guide rail.
  • the above-described main shaft 57 is a hollow cylindrical body.
  • An inner shaft (not shown) is a shaft that can move in the vertical direction inside the main shaft 57.
  • the upper end portion of the inner shaft passes through the main shaft 57 and is connected to the rack gear 43.
  • a bearing (not shown) is provided at the left end of the T-shaped plate. Inside the bearing, the lower end portion of the inner shaft is rotatably held.
  • a stepping motor 51 for moving the T-shaped plate up and down is fixed in front of the T-shaped plate.
  • the shaft 58 of the stepping motor 51 protrudes rearward, that is, downward in FIG.
  • a disc-shaped cam plate (not shown) is fixed to the tip of the shaft 58.
  • a cylindrical projection (not shown) is provided on the rear surface of the cam plate.
  • the tip of the protrusion is inserted into a groove (not shown). The protrusion can slide in the groove.
  • the angle changing mechanism 34 has a pair of L-shaped plates 60 fixed to the upper surface of the turntable 33. Each L-shaped plate 60 extends upward from a base portion fixed in the vicinity of the center of the turntable 33, and its upper end portion extends outward in the radial direction of the turntable 33.
  • a rack gear 43 (not shown) fixed to the inner shaft is provided between the pair of L-shaped plates 60.
  • the rack gear 43 is a metal plate-like member that is long in the vertical direction, and gears are respectively carved on both end faces.
  • a horizontal support shaft 46 having a gear 45 is rotatably supported at the distal end side in the extending direction of each L-shaped plate 60.
  • the support shaft 46 is fixed to the inspection chip 2 via a mounting holder (not shown). For this reason, the inspection chip 2 also rotates around the support shaft 46 in conjunction with the rotation of the gear 45.
  • a pinion gear 44 supported by an L-shaped plate 60 so as to be rotatable about a horizontal axis (not shown) is interposed.
  • the pinion gear 44 meshes with the gear 45 and the rack gear 43, respectively. In conjunction with the vertical movement of the rack gear 43, the pinion gear 44 and the gear 45 are driven to rotate, and the inspection chip 2 is rotated about the support shaft 46.
  • the inspection chip 2 rotates about the main shaft 57, which is a vertical axis, and a centrifugal force acts on the inspection chip 2.
  • the rotation around the vertical axis A1 of the inspection chip 2 is referred to as revolution.
  • the inspection chip 2 rotates about the support shaft 46 which is a horizontal axis, and the direction of the centrifugal force acting on the inspection chip 2 changes relatively.
  • the rotation around the horizontal axis A2 of the inspection chip 2 is called autorotation.
  • the rack gear 43 is also lowered to the lowermost end of the movable range.
  • the inspection chip 2 is in a steady state where the rotation angle is 0 degree.
  • the rack gear 43 is also raised to the uppermost end of the movable range.
  • inspection chip 2 will be in the state rotated 180 degree
  • the upper housing 30 has a box-like frame structure in which frame members are combined, and is installed on the upper left side of the upper plate 32. More specifically, the upper housing 30 is provided outside the range in which the inspection chip 2 is rotated as viewed from the main shaft 57 at the rotation center of the turntable 33.
  • the measurement unit 7 provided in the upper housing 30 includes a light source 71 that emits measurement light, and an optical sensor 72 that detects the measurement light emitted from the light source 71.
  • the light source 71 and the optical sensor 72 are disposed on both the front and rear sides of the turntable 33 outside the rotation range of the inspection chip 2.
  • the position on the left side of the main shaft 57 in the reciprocable range of the inspection chip 2 is the measurement position at which the inspection chip 2 is irradiated with the measurement light.
  • the measurement light connecting the light source 71 and the optical sensor 72 intersects the front surface and the rear surface of the inspection chip 2 substantially perpendicularly.
  • the control device 90 includes a CPU 91 that performs main control of the inspection device 1, a RAM 92 that temporarily stores various data, and a ROM 93 that stores a control program. Connected to the CPU 91 are an operation unit 94 for a user to input instructions to the control device 90, a hard disk device 95 for storing various data and programs, and a display 96 for displaying various information.
  • a personal computer may be used, or a dedicated control device may be used.
  • a revolution controller 97 controls the revolution of the inspection chip 2 by transmitting a control signal for rotating the spindle motor 35 to the spindle motor 35.
  • the rotation controller 98 controls the rotation of the inspection chip 2 by transmitting a control signal for rotating the stepping motor 51 to the stepping motor 51.
  • the measurement controller 99 performs the optical measurement of the inspection chip 2 by driving the measurement unit 7. Specifically, the measurement controller 99 transmits a control signal for executing light emission of the light source 71 and light detection of the optical sensor 72 to the light source 71 and the optical sensor 72.
  • the CPU 91 controls the revolution controller 97, the rotation controller 98, and the measurement controller 99.
  • FIG. 2 Structure of inspection chip 2> With reference to FIG.2 and FIG.3, the detailed structure of the test
  • the upper, lower, left, right, front side, and back side of FIG. 2 are the upper, lower, left, right, front, and rear sides of the inspection chip 2, respectively. .
  • the inspection chip 2 has a square shape including an upper side portion 21, a right side portion 22, a left side portion 23, and a lower side portion 24 when viewed from the front as an example, and has a predetermined thickness.
  • the front surface 201 of the plate member 20 is sealed with a sheet 291 made of a transparent synthetic resin thin plate.
  • the rear surface 202 opposite to the front surface 201 is sealed with a sheet 292 made of a transparent synthetic resin thin plate.
  • a liquid flow path 25 is formed between the plate material 20 and the sheet 291 and between the plate material 20 and the sheet 292 so that the liquid sealed in the inspection chip 2 can flow.
  • the liquid channel 25 is a recess formed at a predetermined depth on the front surface 201 side and the rear surface 202 side of the plate material 20, and extends in a direction orthogonal to the front-rear direction, which is the thickness direction of the plate material 20.
  • the sheets 291 and 292 seal the flow path forming surface of the plate material 20. The sheets 291 and 292 are not shown except for FIGS.
  • the liquid channel 25 includes the sample quantitative channel 11, the reagent quantitative channels 13, 15, the first connection channel 301, the second connection channel 331, the mixing unit 80, the measurement unit 81, and the like.
  • the reagent fixed amount flow path 13 is provided in the upper left part of the front surface 201.
  • the sample quantitative flow path 11 is provided on the right side of the reagent quantitative flow path 13 in the front surface 201.
  • the reagent fixed amount flow path 15 is provided in the upper left part on the rear surface 202 side.
  • the mixing unit 80 is provided in the lower right part of the front surface 201.
  • the mixing unit 80 is an area including a flow path on the right side of an inflow port 306 described later, which is connected to a passage 117 described later and extends downward.
  • the measurement unit 81 is a lower part of the mixing unit 80.
  • the reagent quantification flow paths 13 and 15 include the injection port 130, the reagent injection part 131, the communication path 154, the first holding part 132, the second holding part 133, and the reagent quantifying part 134, respectively. 1st guide part 138, 2nd guide part 137, and reagent surplus part 136 are included.
  • the reagent injection part 131 is provided in the upper left part of the test chip 2.
  • the reagent injection part 131 is a recess that opens upward.
  • the inlet 130 penetrates the plate member 20 from the upper part of the reagent injection part 131 toward the upper side part 21 of the test chip 2.
  • the inlet 130 is a part where the first reagent 18 or the second reagent 19 is injected into the reagent injection part 131.
  • the reagent injection part 131 of the reagent fixed amount flow channel 13 is a part where the first reagent 18 injected from the injection port 130 of the reagent fixed amount flow channel 13 is stored.
  • the reagent injection part 131 of the reagent fixed amount flow channel 15 is a part where the second reagent 19 injected from the injection port 130 of the reagent fixed amount flow channel 15 is stored.
  • the 2nd reagent 19 of this embodiment is a reagent mixed after the 1st reagent 18 and the separation component 17A mentioned later are mixed.
  • the first reagent 18 and the second reagent 19 are collectively referred to as “reagent 16” when not specified either.
  • the first holding part 132 is a concave part that opens to the left, which is a direction that intersects the upward direction that is the opening direction of the reagent injection part 131.
  • the first holding part 132 and the reagent injection part 131 are connected via a communication passage 154 extending in the left-right direction.
  • the first holding part 132 includes a flow path extending downward from the right side portion of the communication path 154 on the left side, and is connected to the second holding part 133 via this flow path.
  • the “direction intersecting the opening direction of the reagent injecting portion 131” refers to a “direction toward the left direction parallel to the extending direction of the upper side portion 21”.
  • a second holding part 133 is formed below the first holding part 132.
  • maintenance part 132 forms the communicating path 154, and is a recessed part formed from the lower wall surface 21A of the upper side part 21 connected with the vertical wall surface 132B, the first wall surface 132A, and the vertical wall surface 132B.
  • the first wall surface 132A is a wall surface facing the lower wall surface 21A.
  • the vertical wall surface 132B is a wall surface connecting the one end portion of the first wall surface 132A and the upper side portion 21.
  • the second holding part 133 is a bent wall surface that opens in a direction intersecting the opening direction of the reagent injection part 131.
  • the second holding unit 133 is connected to the fifth wall surface 133A inclined toward the reagent quantitative unit 134 provided in the lower left portion of the test chip 2 and one end of the fifth wall surface 133A, and the first holding unit 133 It is formed from a sixth wall surface 133 ⁇ / b> B extending in the direction of the portion 132.
  • the second holding portion 133 has a fifth wall surface 133A extending from the bending point 133G with the sixth wall surface 133B toward the lower left direction, and the sixth wall surface 133B having an upper right position from the bending point 133G with the fifth wall surface 133A. It is a bent wall surface formed by extending in the direction.
  • the first wall surface 132A of the first holding portion 132 extends in the lower left direction from the connection portion with the vertical wall surface 132B. Therefore, the first tip 132C in the lower left direction of the first wall surface 132A is located closer to the second holding portion 133 than the connection portion between the first wall surface 132A and the vertical wall surface 132B in the opening direction. Moreover, the 1st holding
  • the first wall 27 is provided between the reagent injection part 131 and the first holding part 132.
  • the first wall portion 27 extends obliquely upward to the right from the left side portion 23 toward the upper side portion 21 to form a reagent injection portion 131.
  • a reagent quantitative unit 134 is provided below the second holding unit 133.
  • the reagent quantification part 134 is a part where the reagent 16 is quantified, and is a concave part recessed in the lower left.
  • the reagent quantification unit 134 is connected to the mixing unit 80 through the first guide unit 138 and the first connection channel 301, and is connected to the reagent surplus unit 136 through the second guide unit 137.
  • the end on the mixing unit 80 side of the reagent quantitative unit 134 is referred to as a first end 141.
  • the end of the reagent quantification unit 134 opposite to the mixing unit 80 is referred to as a second end 142.
  • a surface connecting the first end portion 141 and the second end portion 142 is a reagent fixed amount surface 146.
  • the reagent quantification surface 146 is a virtual surface that is the position of the upper surface of the reagent 16 when the reagent 16 is quantified by the reagent quantification unit 134. Therefore, the volume of the liquid channel 25 below the reagent quantification surface 146 is the quantification amount in the reagent quantification unit 134.
  • the first guide unit 138 extends obliquely upward to the right from the upper part of the reagent quantification unit 134. That is, the first guide portion 138 extends from the first end portion 141 toward the first connection channel 301.
  • the first guide unit 138 is a flow path through which the first reagent 18 quantified by the reagent quantification unit 134 moves.
  • the first guide portion 138 is formed of a bent fourth wall surface 145 connected to the first end portion 141, and a second wall surface 149 that faces the fourth wall surface 145 and that is the wall surface on the second holding portion 133 side.
  • the second guide part 137 extends obliquely downward to the left from the upper part of the reagent quantification part 134. That is, the second guide part 137 extends from the second end part 142 toward the reagent surplus part 136.
  • the second guide unit 137 is a flow path through which the reagent 16 overflowing from the reagent quantitative unit 134 moves.
  • a reagent surplus part 136 is provided at the lower left of the reagent quantification part 134.
  • the reagent surplus part 136 is a part in which the reagent 16 that has moved through the second guide part 137 is accommodated, and is a concave part provided downward and rightward from the lower end part of the second guide part 137.
  • the third wall surface 144 extends from the second end portion 142 of the reagent fixed amount portion 134A in the direction of the reagent surplus portion 136.
  • the reagent 16 overflowing from the reagent quantitative unit 134A flows on the third wall surface 144 and flows into the reagent surplus unit 136.
  • the first connection channel 301 will be described.
  • the reagent quantitative unit 134 of the reagent quantitative channel 13 is referred to as a reagent quantitative unit 134A
  • the reagent quantitative unit 134 of the reagent quantitative channel 15 is referred to as a reagent quantitative unit 134B.
  • the first connection channel 301 is a channel formed on the front surface 201 and connecting the first guide part 138 and the mixing part 80.
  • the first connection channel 301 extends obliquely downward to the right from the tip 310 of the fourth wall surface 145 of the first guide 138 and further extends to the right from the lower end.
  • the first connection channel 301 is formed by the seventh wall surface 302 and the eighth wall surface 303.
  • the seventh wall surface 302 extends from the distal end portion 310 of the fourth wall surface 145 of the first guide portion 138 to a right end portion 313 that forms an inflow port 306 described later.
  • the eighth wall surface 303 is a wall surface facing the seventh wall surface 302 and extending to the mixing unit 80 side.
  • the first connection channel 301 includes a reagent receiving unit 305 and is connected to the inflow port 306.
  • the reagent receiving unit 305 is provided on the lower side 24 side of the first connection channel 301 and is connected to the inflow port 306.
  • the inflow port 306 is formed by the right end portion 313 of the seventh wall surface 302 and the right end portion 314 of the eighth wall surface 303 positioned above the right end portion 313.
  • the inflow port 306 is located on the left side of the mixing unit 80 and is a part for allowing the reagent 16 to flow into the mixing unit 80.
  • a confluence hole portion 351 is provided on the left side of the reagent receiving portion 305.
  • the merge hole 351 is a hole that penetrates the plate member 20 in the front-rear direction and joins the second connection channel 331 to the first connection channel 301.
  • the second connection channel 331 will be described. As shown in FIG. 3, the second connection channel 331 is formed on the rear surface 202 and extends from the reagent quantification unit 134B to the merging hole 351 side, and is a channel that connects the reagent quantification unit 134B and the merging hole 351. is there.
  • the second connection channel 331 includes two reagent receiving portions 341 and 342.
  • the reagent receiving parts 341 and 342 are parts that receive the second reagent 19 quantified by the reagent quantifying part 134B.
  • the second connection channel 331 extends obliquely upward to the right from the reagent determination unit 134B and is connected to the reagent receiver 341, and extends obliquely downward to the left from the reagent receiver 341 and is connected to the reagent receiver 342.
  • the right end portion of the reagent receiving portion 342 is connected to the merge hole portion 351 and is connected to the first connection channel 301 on the front surface 201 side.
  • the sample fixed amount flow path 11 includes an injection port 110, a sample injection unit 111, a first sample holding unit 112, a sample guide unit 113, a separation unit 124, a channel 125, a channel 127, a sample surplus unit 126, A second sample holding unit 123, a sample determination unit 114, a passage 115, a passage 117, and a second surplus unit 116 are included.
  • the sample injection part 111 is provided on the right side of the first holding part 132 of the reagent fixed amount flow path 13.
  • the specimen injection unit 111 is a recess that opens upward.
  • the injection port 110 penetrates the plate material 20 from the upper part of the specimen injection part 111 toward the upper side part 21 of the test chip 2.
  • the injection port 110 is a part where the sample 17 is injected into the sample injection unit 111.
  • the specimen injection unit 111 is a part where the specimen 17 injected from the injection port 110 is stored.
  • the specimen 17 of the present embodiment is a liquid containing components such as blood, plasma, blood cells, bone marrow, urine, vaginal tissue, epithelial tissue, tumor, semen, saliva, or foodstuff.
  • the first sample holding unit 112 and the sample injection unit 111 are connected via a communication path extending in the left-right direction.
  • the first sample holder 112 is a recess that opens in the direction of the sample injector 111.
  • a lower end portion of the first sample holding unit 112 is connected to a sample guide unit 113 which is a passage having a narrow channel.
  • a separation unit 124 is provided below the sample guide unit 113.
  • the sample guide unit 113 guides the sample 17 to the separation unit 124.
  • the separation unit 124 is a part where components contained in the specimen 17 are separated.
  • the separation unit 124 centrifuges the specimen 17 into a component having a small specific gravity and a component having a large specific gravity by the action of centrifugal force.
  • a component having a small specific gravity of the specimen 17 separated by the separation unit 124 is referred to as a separation component 17A
  • a component having a large specific gravity is referred to as a residual component 17B.
  • connection channel 120 extends obliquely upward to the right from the central portion in the vertical direction on the right side surface of the separation unit 124, and the upper end of the connection channel 120 is connected to the upper end of the component holding unit 121.
  • the component holding unit 121 is a storage unit that holds at least a part of the residual component 17 ⁇ / b> B separated in the separation unit 124. That is, the specimen 17 before separation or the separated component 17A may flow into the component holding unit 121.
  • the passage 125 extends obliquely to the left and the passage 127 extends obliquely upward to the right.
  • the passage 125 extends to the specimen surplus portion 126 provided on the lower left side of the separation portion 124.
  • the specimen surplus part 126 is a part where the specimen 17 overflowing from the separation part 124 is stored.
  • the passage 127 is connected to the second sample holder 123.
  • a sample quantitative unit 114 is provided below the second sample holding unit 123.
  • the specimen quantification unit 114 is a part that quantifies the separated component 17A, and is a recess that opens upward.
  • the specimen quantification unit 114 is connected to the mixing unit 80 via the passage 117 and is connected to the second surplus portion 116 via the passage 115.
  • the end on the mixing unit 80 side of the sample quantitative unit 114 is referred to as a first sample quantitative unit end 118.
  • the end of the sample quantitative unit 114 opposite to the mixing unit 80 is referred to as a second sample quantitative unit end 119.
  • a surface connecting the first sample quantitative portion end 118 and the second sample quantitative portion end 119 is a sample quantitative surface 129.
  • the sample quantification surface 129 is a virtual surface serving as the position of the upper surface of the separation component 17A when the separation component 17A is quantified by the sample quantification unit 114. Therefore, the volume of the liquid channel 25 below the sample quantification surface 129 is the quantification amount in the sample quantification unit 114.
  • the sample quantification unit 114 is formed below the sample quantification surface 129 and shallower than the upper side.
  • the second surplus part 116 is a part where the separated component 17A overflowing from the specimen quantification part 114 is stored.
  • the mixing unit 80 is connected to the sample quantification unit 114 via the passage 117.
  • the mixing unit 80 is connected to the reagent quantitative unit 134A via the first connection channel 301.
  • the mixing unit 80 is connected to the reagent quantification unit 134B via the second connection channel 331.
  • the separated component 17A quantified in the sample quantification unit 114, the first reagent 18 quantified in the reagent quantification unit 134A, and the second reagent 19 quantified in the reagent quantification unit 134B are mixed.
  • the measurement light is transmitted to the measurement unit 81 that forms the lower part of the mixing unit 80.
  • the first wall surface 132A of the first holding portion 132 has a first tip portion 132C that is an end portion on the second holding portion 133 side.
  • the sixth wall surface 133B of the second holding part 133 has a second tip part 133C that is an end part on the first holding part 132 side.
  • the first tip portion 132C is provided at a position closer to the reagent injection portion 131 in the crossing direction than the second tip portion 133C.
  • the first reagent 18 flows from the first holding unit 132 to the second holding unit 133 in the flow path between the reagent injection unit 131 and the reagent quantitative unit 134A. Further, since the first tip portion 132C is closer to the reagent injection portion 131 in the crossing direction than the second tip portion 133C, the first reagent 18 flowing out from the first holding portion 132 flows into the second holding portion 133 and others The possibility of flowing into this part can be reduced.
  • the front surface 201 of the test chip 2 is provided with a third holding portion 160 that is a recess that opens in a crossing direction from between the first tip portion 132C and the second tip portion 133C.
  • a third holding portion 160 that is a recess that opens in a crossing direction from between the first tip portion 132C and the second tip portion 133C.
  • the third holding unit 160 includes a first region 161 that extends in the direction opposite to the intersecting direction and a second region 162 that extends in the direction opposite to the upper side, which is the opening direction of the reagent injection unit 131. Therefore, the first reagent 18 that has flowed into the third holding unit 160 remains in the first region 161 when a centrifugal force is applied in the right direction, which is the direction opposite to the intersecting direction.
  • the first reagent 18 that has flowed into the third holding unit 160 remains in the second region 162 when a centrifugal force is applied in a downward direction that is opposite to the upper side that is the opening direction of the reagent injection unit 131. . Therefore, the possibility that the first reagent 18 that has flowed into the third holding unit 160 flows out of the third holding unit 160 can be reduced.
  • a volume V ⁇ b> 1 surrounded by the wall surfaces 27 ⁇ / b> A and 27 ⁇ / b> B forming the reagent injection portion 131 and the first virtual surface 170 passing through the tip portion 27 ⁇ / b> C of the first wall portion 27 is shown in FIG. 2.
  • the volume V2 which is a predetermined injection amount injected into the reagent injection part 131.
  • the wall surface 27 ⁇ / b> A and the wall surface 27 ⁇ / b> B are wall surfaces on the reagent injection part 131 side of the first wall part 27.
  • the wall surface 27A extends from the connecting portion with the wall surface 27B to the tip portion 27C in the upper right direction.
  • the wall surface 27B extends in the upper left direction from the connection portion with the wall surface 27A.
  • the distal end portion 27 ⁇ / b> C is a distal end in the extending direction of the first virtual surface 170.
  • the volume V1, which is a predetermined injection amount, is determined by, for example, injecting a predetermined volume V1 from the injection port 130 into the reagent injection unit 131 using a pipette.
  • a line or a mark indicating the volume V1 which is a predetermined injection amount, may be formed in the reagent injection part 131. Therefore, the first reagent 18 when the first reagent 18 starts to flow out from the distal end portion 27C flows out in the direction of the first virtual line 171 and hits the first wall surface 132A. Therefore, it is possible to reduce the possibility that the first reagent 18 that has flowed out of the reagent injection unit 131 flows out to the second holding unit 133 or the reagent quantitative unit 134A without the first holding unit 132.
  • the first wall portion 27 is formed with a distance L1 from the first tip portion 132C on the first wall portion 27 side of the first wall surface 132A. That is, the rightmost end portion 27D of the first wall portion 27 is formed with a distance L1 from the first tip portion 132C. Therefore, in the crossing direction, the end portion 27D is formed with a gap L1 from the first tip portion 132C on the first wall portion 27 side of the first wall surface 132A.
  • the reagent 18 flows from the gap L1 toward the second holding unit 133. Therefore, it is possible to reduce the possibility that the first reagent 18 overflowing from the first holding part 132 flows back to the reagent injection part 131. Therefore, it is possible to prevent the quantitative amount from being reduced below the specified value, and improve the quantitative accuracy.
  • the angle on the first wall portion 27 side formed by the intersecting direction and the first wall surface 132A of the first holding portion 132 is the first wall surface angle ⁇ 1.
  • the first wall surface angle ⁇ 1 is an acute angle.
  • the angle formed between the intersecting direction and the fifth wall surface 133A on the side opposite to the third holding portion 160 is the fifth wall surface angle ⁇ 2.
  • the fifth wall surface angle ⁇ 2 is an acute angle.
  • the first wall surface 132A and the fifth wall surface 133A are formed so that the first wall surface angle ⁇ 1 is equal to or smaller than the fifth wall surface angle ⁇ 2.
  • the angle on the fourth wall surface 145 side formed by the second virtual line 172 and the intersecting direction is the fourth wall surface angle ⁇ 3.
  • the fourth wall surface angle ⁇ 3 is an acute angle.
  • the second imaginary line 172 is a tangent line between the second end 142 of the reagent quantitative unit 134A and the tip 310 of the first guide 138 facing the second wall 149 of the fourth wall 145.
  • the fifth wall surface 133A and the fourth wall surface 145 are formed so that the fifth wall surface angle ⁇ 2 is smaller than the fourth wall surface angle ⁇ 3. Accordingly, since the first wall surface angle ⁇ 1 is equal to or smaller than the fifth wall surface angle ⁇ 2, the first reagent 18 that has flowed out of the first holding unit 132 during the injection of the first reagent 18 into the reagent quantitative unit 134A is It can flow into the reagent quantification unit 134A without remaining in the holding unit 133.
  • the fifth wall surface angle ⁇ 2 is smaller than the fourth wall surface angle ⁇ 3
  • the first reagent 18 moves to the tip of the fourth wall surface 145.
  • the possibility of getting over the portion 310 can be reduced. Therefore, the possibility that the first reagent 18 is less than the prescribed amount to be quantified can be reduced and the quantification accuracy can be improved.
  • the second holding portion 133 includes a sixth wall surface 133B that extends from the bending point 133G toward the second tip portion 133C.
  • a volume surrounded by the first wall surface 132A and the vertical wall surface 132B forming the first holding portion 132 and the second virtual surface 132D passing through the first tip portion 132C is a volume V2.
  • the volume V2 is the same as the volume V1 of the predetermined injection amount into the reagent injection part 131
  • the first tip part 132C is brought into contact with the outflow direction which is a direction perpendicular to the second virtual surface 132D.
  • the drawn third virtual line 173 intersects the sixth wall surface 133B.
  • the first reagent 18 when the first reagent 18 is injected from the first holding unit 132 to the second holding unit 133, the first reagent 18 moves in the direction of the sixth wall surface 133 ⁇ / b> B of the second holding unit 133 along the third virtual line 173. Since it flows, the possibility that the first reagent 18 flows into the third holding unit 160 can be reduced.
  • the first wall surface 132A and the second holding portion of the first holding portion 132 have an obtuse angle ⁇ 4 on the second holding portion 133 side.
  • a sixth wall surface 133B of 133 is formed. Therefore, when the first reagent 18 is injected from the first holding unit 132 into the second holding unit 133, the first reagent 18 flows into the third holding unit 160, and at the time of quantification in the downstream reagent quantification unit 134. The possibility that the first reagent 18 is insufficient can be reduced.
  • the support shaft 46 extending from the L-shaped plate 60 is vertically connected to the center of the rear surface of the plate member 20 via a mounting holder (not shown). As the support shaft 46 rotates, the inspection chip 2 rotates around the support shaft 46.
  • the inspection chip 2 is in the steady state shown in FIGS. 2 and 3, the upper side 21 and the lower side 24 are orthogonal to the direction of gravity G, the right side 22 and the left side 23 are parallel to the direction of gravity G, and The left side portion 23 is disposed closer to the main shaft 57 than the right side portion 22.
  • the inspection apparatus 1 performs inspection by optical measurement by allowing the measurement light connecting the light source 71 and the optical sensor 72 to pass through the measurement unit 81.
  • Example of inspection method> An inspection method using the inspection apparatus 1 and the inspection chip 2 will be described.
  • the sample 17 is injected from the injection port 110 and placed in the sample injection unit 111.
  • the first reagent 18 is injected from the injection port 130 of the reagent fixed amount flow path 13 and is arranged in the reagent injection portion 131 of the reagent fixed amount flow path 13.
  • the second reagent 19 is injected from the inlet 130 of the reagent quantitative flow channel 15 and is arranged in the reagent injection part 131 of the reagent quantitative flow channel 15.
  • the arrangement method of the first reagent 18, the second reagent 19, and the specimen 17 is not limited.
  • holes are opened at positions corresponding to the sample injection unit 111 and the reagent injection unit 131 in the sheets 291 and 292, and the user injects the sample 17, the first reagent 18, and the second reagent 19 from the holes, You may seal and seal.
  • the first reagent 18 and the second reagent 19 may be arranged in advance in the respective reagent injection portions 131 of the reagent quantitative flow paths 13 and 15 and sealed with sheets 291 and 292.
  • a hole may be opened in the sheet 291 at a position corresponding to the sample injection part 111 of the sample fixed amount flow channel 11, and the user may inject the sample 17 from the hole, and further seal and seal.
  • the volume V1 that is a predetermined injection amount of the first reagent 18 is determined, for example, by the user injecting the predetermined volume V1 into the reagent injection unit 131 using a pipette.
  • the injection amounts of the second reagent 19 and the specimen 17 are also determined, for example, by the user injecting a predetermined volume into the reagent injection part 131 and the specimen injection part 111 using a pipette.
  • the user attaches the inspection chip 2 to a mounting holder (not shown) and inputs a processing start command from the operation unit 94.
  • the CPU 91 executes the centrifugal process shown in FIG. 5 based on the control program stored in the ROM 93.
  • the inspection apparatus 1 can inspect two inspection chips 2 at the same time. For convenience of explanation, a procedure for inspecting one inspection chip 2 will be described below.
  • the steady state of the inspection chip 2 shown in FIGS. 2 and 3 is referred to as a rotation angle of 0 degree
  • the state rotated 90 degrees counterclockwise from the steady state is referred to as a rotation angle of 90 degrees.
  • the inspection chip 2 rotates counterclockwise as viewed from the front. Further, when the CPU 91 rotates the inspection chip 2 from 90 degrees to 90 degrees, the inspection chip 2 rotates clockwise as viewed from the front.
  • the CPU 91 reads the motor drive information stored in advance in the HDD 95, sets the drive information of the spindle motor 35 in the revolution controller 97, and sets the drive information of the stepping motor 51 in the rotation controller 98. (S1). At this time, the test chip 2 is in a steady state and has a rotation angle of 0 degree as shown in FIGS. Next, the CPU 91 shown in FIG. 1 controls the revolution controller 97 to start driving the spindle motor 35 (S2). As a result, the inspection chip 2 having a rotation angle of 0 degrees revolves. The spindle motor 35 increases the rotation speed of the turntable 33 to the speed V based on an instruction from the revolution controller 97.
  • the speed V is, for example, 3000 rpm.
  • centrifugal force X acts on the inspection chip 2.
  • the CPU 91 maintains the rotation speed of the spindle motor 35 at the speed V (S3).
  • centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the reagent 16 moves from the reagent injection part 131 to the first holding part 132 by the action of the centrifugal force X.
  • the sample 17 moves from the sample injection unit 111 to the first sample holding unit 112.
  • the rotation speed of the turntable 33 is assumed to be constant at the speed V, but the value of the speed V may be changed during the centrifugal process.
  • FIG. 6C shows a state in which the inspection chip 2 has been rotated to a rotation angle of 90 degrees
  • FIG. 6B shows an intermediate state during the rotation of the inspection chip 2 from the rotation angle of 0 degrees to 90 degrees of rotation. Indicates the state.
  • the inspection chip 2 is rotated up to 90 degrees of rotation, and the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24.
  • the reagent 16 flows from the first holding unit 132 to the reagent quantifying unit 134 via the second holding unit 133 that is a bent wall surface.
  • the excess reagent 16 in the reagent quantitative unit 134 flows to the reagent surplus unit 136 via the second guide unit 137.
  • the centrifugal force X acts in the direction perpendicular to the reagent fixed amount surface 146. Thereby, the reagent 16 for the capacity of the reagent quantification unit 134 is quantified.
  • the sample 17 flows from the first sample holding unit 112 to the separation unit 124 via the sample guide unit 113.
  • the excess specimen 17 in the separation unit 124 flows to the specimen surplus part 126 via the passage 125.
  • the capacity of the separation part 124 is the capacity of the liquid flow path 25 below the virtual surface 148 extending in the right direction from the end part 147 on the passage 125 side in the separation part 124 shown in FIG.
  • the first reagent 18 during the rotation of the test chip 2 from the rotation angle 0 degree shown in FIG. 6 (A) to the rotation angle 90 degree shown in FIG. 6 (C).
  • the flow will be described.
  • the first reagent 18 is transferred from the first holding part 132 to the second holding part 133 while the test chip 2 is rotated from the rotation angle of 0 degree to the rotation angle of 90 degrees.
  • the second holding unit 133 is a bent wall surface, the first reagent 18 flows from the second holding unit 133 into the reagent quantitative unit 134A without being stored.
  • the flow path from the reagent injection unit 131 to the reagent quantification unit 134A becomes long, and the first The amount that the reagent 18 is held increases. Therefore, even if the injected first reagent 18 is large, the injected first reagent 18 is held in the plurality of holding parts of the first holding part 132 and the second holding part 133. Therefore, the possibility that the first reagent 18 flows into the first guide unit 138 or the second guide unit 137 without entering the reagent quantitative unit 134A can be reduced. The same applies to the front surface 201 on the rear surface 202 side.
  • the first tip 132C on the second holder 133 side of the first holder 132 is closer to the reagent injection part 131 in the crossing direction than the second tip 133C on the first holder 132 side of the second holder 133. Since it is near, the 1st reagent 18 which flowed out from the 1st holding
  • the one reagent 18 can flow into the reagent quantitative unit 134A without remaining in the second holding unit 133. Further, since the fifth wall surface angle ⁇ 2 is smaller than the fourth wall surface angle ⁇ 3, when the first reagent 18 is injected from the second holding unit 133 into the reagent quantification unit 134A, the first reagent 18 moves to the tip of the fourth wall surface 145. The possibility of getting over the portion 310 can be reduced. Therefore, the possibility that the first reagent 18 is less than the prescribed amount to be quantified can be reduced, and the quantification accuracy can be improved. The same applies to the front surface 201 on the rear surface 202 side.
  • the CPU 91 holds the rotational speed of the spindle motor 35 at the speed V for a predetermined time (S5).
  • the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24 for a predetermined time on the inspection chip 2 having a rotation angle of 90 degrees shown in FIG.
  • the separation unit 124 the component of the specimen 17 is separated into the separation component 17A and the residual component 17B.
  • the specimen 17 is blood
  • blood cells having a large specific gravity accumulate on the side in which the centrifugal force X acts
  • plasma having a small specific gravity accumulates on the side opposite to the direction in which the centrifugal force X acts. That is, the residual component 17B that is blood cells in the blood and the separation component 17A that is plasma are separated.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 up to the rotation angle of 0 degrees as shown in FIG. 7B (S6).
  • the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the first reagent 18 quantified in the reagent quantification unit 134A moves to the mixing unit 80 and is stored.
  • the second reagent 19 quantified in the reagent quantification unit 134B moves to the reagent receiving unit 341.
  • the separation component 17A moves to the second specimen holding unit 123 through the passage 127.
  • the separated component 17A remaining in the separation unit 124 and a part of the residual component 17B move to the component holding unit 121 via the connection channel 120.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 8A (S7).
  • the centrifugal force X acts from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the separation component 17A flows from the second sample holding unit 123 to the sample quantification unit 114. Further, the second reagent 19 moves from the reagent receiving part 341 to the reagent receiving part 342. As shown in FIG. 8B, the excess separated component 17A in the specimen quantification unit 114 flows to the second surplus unit 116 via the passage 115.
  • the centrifugal force X acts in a direction perpendicular to the specimen quantification surface 129.
  • the separation component 17A corresponding to the volume of the specimen quantification unit 114 is quantified.
  • the second reagent 19 that has moved to the reagent receiving part 342 joins the first connection channel 301 formed in the front surface 201 via the joining hole part 351.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51. As shown in FIG. 8C, FIG. 9A, and FIG. Is rotated (S8). As a result, the centrifugal force X acts on the test chip 2 from the left side 23 toward the right side 22.
  • the centrifugal force X acts in the process of changing the posture of the test chip 2 from the state shown in FIG. 8B to the state shown in FIG. 9B, as shown in FIG.
  • the separated component 17A quantified by the sputum sample quantifying unit 114 flows into the first reagent 18 stored in the first reagent 18, and is mixed to generate a mixed liquid 261.
  • the second reagent 19 that has joined from the joining hole portion 351 flows into the mixing portion 80, and a second mixed liquid 262 is generated as shown in FIG. 9B.
  • the CPU 91 controls the rotation controller 98 to drive and control the stepping motor 51 to rotate the inspection chip 2 to a rotation angle of 90 degrees as shown in FIG. 9C (S9).
  • the centrifugal force X acts on the inspection chip 2 from the upper side portion 21 toward the lower side portion 24. Due to the action of the centrifugal force X, the second mixed liquid 262 moves to the measuring unit 81.
  • the CPU 91 controls the rotation controller 98 to drive the stepping motor 51.
  • the CPU 91 rotates the inspection chip 2 until the rotation angle is 0 degree (S10). Further, the CPU 91 controls the revolution controller 97 to stop the rotation of the spindle motor 35 (S10). Therefore, the revolution of the inspection chip 2 is completed. Centrifugation is terminated.
  • the CPU 91 controls the revolution controller 97 to rotate and move the inspection chip 2 to the angle of the measurement position.
  • the measurement controller 99 shown in FIG. 1 causes the light source 71 to emit light
  • the measurement light passes through the second mixed liquid 262 stored in the measurement unit 81.
  • the CPU 91 performs optical measurement of the second liquid mixture 262 based on the change amount of the measurement light received by the optical sensor 72, and acquires measurement data.
  • CPU91 calculates the measurement result of the 2nd liquid mixture 262 based on the acquired measurement data.
  • the inspection result of the second mixed liquid 262 based on the measurement result is displayed on the display 96 shown in FIG.
  • the measuring method of the 2nd liquid mixture 262 is not restricted to an optical measurement, Another method may be sufficient.
  • the reagent injection unit 131 is an example of the “injection unit” of the present disclosure.
  • the first holding unit 132 and the second holding unit 133 are examples of the “holding unit” of the present disclosure.
  • the first reagent 18 and the second reagent 19 are examples of the “reagent” of the present disclosure.
  • the reagent quantification unit 134A is an example of the “quantification unit” of the present disclosure.
  • the reagent quantitative unit 134B is an example of the “quantitative unit” of the present disclosure.
  • the first holding unit 132 and the second holding unit 133 that hold the reagent are provided in the flow path between the reagent injection unit 131 and the reagent quantitative unit 134A.
  • a plurality are provided adjacent to each other.
  • the first holding part 132 and the second holding part 133 provided on the downstream side thereof exist, so the path through which the first reagent 18 flows becomes long, The amount that the first reagent 18 is held increases. Therefore, even if the injected first reagent 18 is large, the injected first reagent 18 is held in the plurality of holding parts of the first holding part 132 and the second holding part 133.
  • the possibility that the first reagent 18 flows into the first guide unit 138 or the second guide unit 137 without entering the reagent quantitative unit 134A can be reduced. Therefore, since the amount of the first reagent 18 necessary for quantification is injected into the reagent quantification unit 134A, the quantification accuracy of the first reagent 18 in the reagent quantification unit 134A can be improved.
  • the first reagent 18 may be changed to a specimen.
  • the measurement unit 81 is a lower part of the mixing unit 80, but may be provided separately from the mixing unit 80.
  • the reagent fixed amount flow channel 15 and the second connection flow channel 331 are formed on the rear surface 202 side, and the reagent fixed amount flow channel 13 and the first connection flow channel 301 are formed on the front surface 201.
  • the present invention is not limited to this.
  • the reagent fixed amount flow path 15, the second connection flow path 331, the reagent fixed amount flow path 13, and the first connection flow path 301 may be formed on the front surface 201.
  • the first connection flow path 301 and the second connection flow path 331 may join at the flow path of the front surface 201 instead of joining at the joining hole portion 351.
  • the reagent quantitative flow channel 15 and the second connection flow channel 331 may not be provided in the test chip 2.
  • the reagent quantification unit 134 is a quantification unit that quantifies the reagent, but may be used as a sample quantification unit.
  • maintenance part 133 may be formed from a recessed part instead of a bending wall surface.
  • a ridge line 131A slightly raised from the flow path may be formed in the reagent injection part 131.
  • the volume surrounded by the ridge line 131A and the wall surface 23A, the wall surface 27A, and the wall surface 27B forming the reagent injection part 131 may be a volume V1 of a predetermined injection amount into the reagent injection part 131.
  • a virtual surface 131B orthogonal to the wall surface 23A is formed from the distal end portion 27C of the first wall portion 27 forming the reagent injection portion 131, and the reagent injection portion 131 is formed.
  • the volume surrounded by the wall surface 23A, the wall surface 27A, and the wall surface 27B may be a volume V1 of a predetermined injection amount into the reagent injection part 131. In these cases, it can be clearly seen how far the first reagent 18 (16) is injected into the reagent injection part 131 to reach the volume V1.

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Abstract

L'invention concerne une puce de détection présentant une meilleure précision de fixation de quantité. Une partie d'injection de réactif (131) est une concavité qui s'ouvre vers le haut, et stocke un premier réactif (18) injecté depuis un orifice d'injection (130). Une première partie de maintien (132) forme une concavité qui s'ouvre vers la gauche, qui est une direction qui coupe la direction d'ouverture vers le haut de la partie d'injection de réactif (131). Une seconde partie de maintien (133) est formée sous la première partie de maintien (132). La seconde partie de maintien (133) est formée à partir d'une cinquième surface de paroi (133A) inclinée vers une partie de fixation de quantité de réactif (134) située dans la section gauche inférieure de la puce de détection (2), et une sixième surface de paroi (133B) s'étendant dans la direction de la première partie de maintien (132) et reliée à une première extrémité de la cinquième surface de paroi (133A). La seconde partie de maintien (133) forme une ouverture de surface de paroi incurvée dans une direction qui coupe la direction d'ouverture de la partie d'injection de réactif (131). La partie de fixation de quantité de réactif (134), qui fixe la quantité du premier réactif (18) à injecter depuis la seconde partie de maintien (133), est disposée en dessous de la seconde partie de maintien (133).
PCT/JP2015/060104 2014-03-31 2015-03-31 Puce de détection Ceased WO2015152230A1 (fr)

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CN114313942A (zh) * 2022-01-05 2022-04-12 方运和 一种快速生物检测用全程密封可二次注射的试剂反应盒

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JP2011237325A (ja) * 2010-05-12 2011-11-24 Brother Ind Ltd 検査対象受体
JP2012127724A (ja) * 2010-12-14 2012-07-05 Rohm Co Ltd マイクロチップ
JP2012247342A (ja) * 2011-05-30 2012-12-13 Brother Ind Ltd 検査対象受体、その検査対象受体を備えた液体混合システム、及びその液体混合システムを用いる液体混合方法
JP2013079812A (ja) * 2011-09-30 2013-05-02 Brother Ind Ltd 検査対象受体

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Publication number Priority date Publication date Assignee Title
JP2011237325A (ja) * 2010-05-12 2011-11-24 Brother Ind Ltd 検査対象受体
JP2012127724A (ja) * 2010-12-14 2012-07-05 Rohm Co Ltd マイクロチップ
JP2012247342A (ja) * 2011-05-30 2012-12-13 Brother Ind Ltd 検査対象受体、その検査対象受体を備えた液体混合システム、及びその液体混合システムを用いる液体混合方法
JP2013079812A (ja) * 2011-09-30 2013-05-02 Brother Ind Ltd 検査対象受体

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114313942A (zh) * 2022-01-05 2022-04-12 方运和 一种快速生物检测用全程密封可二次注射的试剂反应盒

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