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WO2013043758A3 - Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products - Google Patents

Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products Download PDF

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Publication number
WO2013043758A3
WO2013043758A3 PCT/US2012/056159 US2012056159W WO2013043758A3 WO 2013043758 A3 WO2013043758 A3 WO 2013043758A3 US 2012056159 W US2012056159 W US 2012056159W WO 2013043758 A3 WO2013043758 A3 WO 2013043758A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
derived
produce
hydroxypropionic acid
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2012/056159
Other languages
French (fr)
Other versions
WO2013043758A2 (en
Inventor
Christopher P. Mercogliano
Faith Dizon WATSON
Tanya E.w. LIPSCOMB
Hans H. Liao
Michael D. Lynch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OPX Biotechnologies Inc
Original Assignee
OPX Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OPX Biotechnologies Inc filed Critical OPX Biotechnologies Inc
Publication of WO2013043758A2 publication Critical patent/WO2013043758A2/en
Publication of WO2013043758A3 publication Critical patent/WO2013043758A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/01075—Malonyl CoA reductase (malonate semialdehyde-forming)(1.2.1.75)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention regards microbial production of malonate semialdehyde, 3-hydroxypropionate, and products derived thereof, including using a combinations of malonyl CoA reductase domains and 3-HP dehydrogenase domains with altered cofactor specificities for NADH and NADPH. Embodiments of the present invention also include methods for engineering these proteins, the genetically engineered microorganisms used for production with these altered enzymes, methods for producing such organisms, and methods of making compounds, downstream compounds, and downstream, products.
PCT/US2012/056159 2011-09-19 2012-09-19 Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products Ceased WO2013043758A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US201161536558P 2011-09-19 2011-09-19
US201161536464P 2011-09-19 2011-09-19
US201161536539P 2011-09-19 2011-09-19
US61/536,558 2011-09-19
US61/536,539 2011-09-19
US61/536,464 2011-09-19

Publications (2)

Publication Number Publication Date
WO2013043758A2 WO2013043758A2 (en) 2013-03-28
WO2013043758A3 true WO2013043758A3 (en) 2013-06-13

Family

ID=47915086

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2012/056159 Ceased WO2013043758A2 (en) 2011-09-19 2012-09-19 Compositions and methods regarding direct nadh utilization to produce 3-hydroxypropionic acid, derived chemicals and further derived products

Country Status (1)

Country Link
WO (1) WO2013043758A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2751261B1 (en) 2011-09-30 2018-08-29 Novozymes, Inc. Dehydrogenase variants and polynucleotides encoding same
KR102098849B1 (en) * 2012-05-30 2020-04-09 란자테크 뉴질랜드 리미티드 Recombinant microorganisms and uses therefor
JP2015536669A (en) 2012-11-30 2015-12-24 ノボザイムス，インコーポレイティド Production of 3-hydroxypropionic acid by recombinant yeast
EP3877525A2 (en) 2018-12-18 2021-09-15 Braskem S.A. Co-production pathway for 3-hp and acetyl-coa derivatives from malonate semialdehyde
AU2022218205A1 (en) 2021-02-08 2023-05-25 Lanzatech, Inc. Recombinant microorganisms and uses therefor
US20250290082A1 (en) * 2021-05-28 2025-09-18 Cargill, Incorporated Fermentation method for producing malonic acid

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638316B2 (en) * 2000-11-20 2009-12-29 Cargill, Incorporated 3-hydroxypropionic acid and other organic compounds
US20110125118A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals
US20120244588A1 (en) * 2011-03-24 2012-09-27 Samsung Electronics Co., Ltd. Method of producing 3-hydroxypropionic acid using malonic semialdehyde reducing pathway

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7638316B2 (en) * 2000-11-20 2009-12-29 Cargill, Incorporated 3-hydroxypropionic acid and other organic compounds
US20110125118A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals
US20120244588A1 (en) * 2011-03-24 2012-09-27 Samsung Electronics Co., Ltd. Method of producing 3-hydroxypropionic acid using malonic semialdehyde reducing pathway

Also Published As

Publication number Publication date
WO2013043758A2 (en) 2013-03-28

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