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IN2012DN00302A - - Google Patents

Info

Publication number
IN2012DN00302A
IN2012DN00302A IN302DEN2012A IN2012DN00302A IN 2012DN00302 A IN2012DN00302 A IN 2012DN00302A IN 302DEN2012 A IN302DEN2012 A IN 302DEN2012A IN 2012DN00302 A IN2012DN00302 A IN 2012DN00302A
Authority
IN
India
Prior art keywords
enzyme
mgs
methylglyoxal synthase
mutant
biochemical
Prior art date
Application number
Inventor
Francois Voelker
Laurence Dumon-Seignovert
Philippe Soucaille
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of IN2012DN00302A publication Critical patent/IN2012DN00302A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03003—Methylglyoxal synthase (4.2.3.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention concerns a method for the production of a biochemical selected among lactic acid, acetol and 1,2-propanediol, comprising culturing a microorganism modified for an improved production of the biochemical selected among lactic acid, acetol and 1,2-propanediol in an appropriate culture medium and recovery of the desired biochemical which may be further purified wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme which activity is not inhibited by orthophosphate. The present invention concerns a mutant methylglyoxal synthase (MGS) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein - the mutant enzyme has retained more than 50% of the methylglyoxal synthase activity of the parent enzyme and - the methylglyoxal synthase activity of the mutant MGS is not inhibited by orthophosphate as compared to the parent enzyme.
IN302DEN2012 2009-07-30 2010-07-30 IN2012DN00302A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23007609P 2009-07-30 2009-07-30
EP09166815 2009-07-30
PCT/EP2010/061094 WO2011012693A1 (en) 2009-07-30 2010-07-30 Mutant methylglyoxal synthase (mgs) for the production of a biochemical by fermentation

Publications (1)

Publication Number Publication Date
IN2012DN00302A true IN2012DN00302A (en) 2015-05-08

Family

ID=41014566

Family Applications (1)

Application Number Title Priority Date Filing Date
IN302DEN2012 IN2012DN00302A (en) 2009-07-30 2010-07-30

Country Status (9)

Country Link
US (1) US8497102B2 (en)
EP (1) EP2459710B1 (en)
JP (1) JP5913099B2 (en)
KR (1) KR101771266B1 (en)
CN (1) CN102575235B (en)
BR (1) BR112012002153A2 (en)
CA (1) CA2767962C (en)
IN (1) IN2012DN00302A (en)
WO (1) WO2011012693A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2532751A1 (en) 2011-06-10 2012-12-12 Metabolic Explorer Use of inducible promoters in the fermentative production of 1,2-propanediol
US9353386B2 (en) 2011-06-15 2016-05-31 B.R.A.I.N. Biotechnology Research And Information Network Ag Means and methods for producing propanediol
JP2014530020A (en) 2011-10-04 2014-11-17 フロリダ大学リサーチファウンデーション インコーポレイティッド Variant of glycerol dehydrogenase having D-lactate dehydrogenase activity and use thereof
CN104812889B (en) * 2012-09-14 2018-08-28 麦兰特公司 Organic acid is generated by fermenting at low PH
CN102994577B (en) * 2012-10-26 2015-01-21 上海交通大学 Method for producing acetone alcohol by microbial cell conversion method
JP6446807B2 (en) * 2014-03-26 2019-01-09 三菱ケミカル株式会社 Method for producing organic compound using microorganism
WO2017042602A1 (en) 2015-09-10 2017-03-16 Metabolic Explorer New lactaldehyde reductases for the production of 1,2-propanediol
EP3342873A1 (en) 2016-12-29 2018-07-04 Metabolic Explorer Conversion of methylglyoxal into hydroxyacetone using enzymes and applications thereof
KR102489629B1 (en) * 2020-04-02 2023-01-18 서강대학교산학협력단 Methylglyoxal synthase from Clostridium difficile 630 strain attatched strep-tag, and method for producing methylglyoxal using thereof
KR102488646B1 (en) * 2020-04-02 2023-01-16 서강대학교산학협력단 Ultra-high heat resistant methylglyoxal synthase from Oceanithermus profundus DSM 14977 strain attatched strep-tag, and method for producing methylglyoxal using thereof
CN119709808B (en) * 2025-03-03 2025-07-25 天津科技大学 Method for synthesizing lactic acid monomer by taking sucrose as raw material

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3336051A1 (en) 1983-10-04 1985-04-25 Maizena Gmbh, 2000 Hamburg Process for the preparation of 1,2-propanediol, especially D(-)-1,2-propanediol
DE3820040A1 (en) 1988-06-13 1989-12-14 Henkel Kgaa METHOD AND DEVICE FOR SEPARATING PROPYLENE GLYCOL
DE4128692A1 (en) 1991-08-29 1993-03-04 Henkel Kgaa METHOD FOR PRODUCING ACETOL
WO1999028481A1 (en) 1997-02-19 1999-06-10 Wisconsin Alumni Research Foundation Microbial production of hydroxyacetone and 1,2-propanediol
US6087140A (en) 1997-02-19 2000-07-11 Wisconsin Alumni Research Foundation Microbial production of 1,2-propanediol from sugar
CN101157930A (en) 2002-05-30 2008-04-09 卡吉尔道有限责任公司 Fermentation process using specific oxygen uptake rates as a process control
US7300787B2 (en) 2002-07-05 2007-11-27 Archer-Daniels-Midland Company Lactobacillus strains and use thereof in fermentation for L-lactic acid production
KR100864672B1 (en) 2003-04-02 2008-10-23 씨제이제일제당 (주) Method for producing 1,2-propanediol using Klebsiella pneumoniae
WO2005033324A1 (en) 2003-09-30 2005-04-14 Mitsui Chemicals, Inc. Biocatalyst for producing d-lactic acid
US20050112737A1 (en) 2003-11-20 2005-05-26 A. E. Staley Manufacturing Co. Lactic acid producing yeast
FR2864967B1 (en) 2004-01-12 2006-05-19 Metabolic Explorer Sa ADVANCED MICROORGANISM FOR THE PRODUCTION OF 1,2-PROPANEDIOL
WO2005095536A2 (en) 2004-03-25 2005-10-13 Suppes Galen J Method of producing lower alcohols from glycerol
EP2305826B1 (en) 2005-08-10 2016-03-30 University of Florida Research Foundation, Inc. Materials and methods for efficient lactic acid production
RU2412929C9 (en) 2005-12-29 2012-04-10 Басф Се Method of extracting propylene glycol from aqueous compositions
EP2139985A1 (en) 2007-03-23 2010-01-06 Metabolic Explorer Metabolically engineered microorganism useful for the production of 1,2-propanediol
US20110262980A1 (en) 2007-03-23 2011-10-27 Philippe Soucaille Micro-organisms for the production of acetol obtained by a combination of evolution and rational design
WO2008116851A1 (en) 2007-03-23 2008-10-02 Metabolic Explorer Metabolically engineered microorganism useful for the production of acetol
DK2109681T3 (en) 2007-03-23 2014-12-08 Metabolic Explorer Sa NEW MICROORGANISMS FOR THE PREPARATION OF 1,2-PROPANDIOL OBTAINED BY A COMBINATION OF EVOLUTION AND RATIONAL DESIGN

Also Published As

Publication number Publication date
JP2013500032A (en) 2013-01-07
BR112012002153A2 (en) 2015-09-15
CA2767962A1 (en) 2011-02-03
WO2011012693A1 (en) 2011-02-03
JP5913099B2 (en) 2016-04-27
EP2459710B1 (en) 2016-11-09
KR20120047276A (en) 2012-05-11
US20120122166A1 (en) 2012-05-17
CN102575235A (en) 2012-07-11
EP2459710A1 (en) 2012-06-06
CA2767962C (en) 2017-11-28
CN102575235B (en) 2015-05-20
KR101771266B1 (en) 2017-08-24
US8497102B2 (en) 2013-07-30

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