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WO2012134262A3 - Procédé d'extraction de protéines à partir d'un matériau végétal riche en pectine - Google Patents

Procédé d'extraction de protéines à partir d'un matériau végétal riche en pectine Download PDF

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Publication number
WO2012134262A3
WO2012134262A3 PCT/MY2012/000044 MY2012000044W WO2012134262A3 WO 2012134262 A3 WO2012134262 A3 WO 2012134262A3 MY 2012000044 W MY2012000044 W MY 2012000044W WO 2012134262 A3 WO2012134262 A3 WO 2012134262A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
inventive method
plant material
preparation
pectin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/MY2012/000044
Other languages
English (en)
Other versions
WO2012134262A2 (fr
Inventor
Zalinah HAJI.AHMAD
Elham RASTEGARI
David Farringdon SPENCER
Maznah Ismail
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universiti Putra Malaysia (UPM)
Original Assignee
Universiti Putra Malaysia (UPM)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiti Putra Malaysia (UPM) filed Critical Universiti Putra Malaysia (UPM)
Publication of WO2012134262A2 publication Critical patent/WO2012134262A2/fr
Publication of WO2012134262A3 publication Critical patent/WO2012134262A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C07K1/128—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general sequencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015—Polygalacturonase (3.2.1.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/0201—Pectin lyase (4.2.2.10)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

L'invention concerne des procédés d'extraction de protéines à partir d'un matériau végétal caractérisé par une forte teneur en composés interférents, en particulier des polysaccharides abondants tels que la pectine. Le procédé de l'invention met en oeuvre un traitement pectolytique, en préparation de l'échantillon de matériau végétal, avant extraction des protéines. Ce procédé optimisé produit des extraits de protéines de haute qualité compatibles avec d'autres analyses protéomiques sensibles, notamment des procédés de type électrophorèse sur gel bidimensionnelle ou spectrométrie de masse. L'invention concerne également les extraits de protéines obtenus par le procédé de l'invention, et l'utilisation de ce procédé dans la préparation d'échantillons de protéines. L'invention concerne en outre un kit d'extraction de protéines pour la mise en œuvre du procédé, ainsi que l'utilisation de ce kit pour l'analyse de protéines et la protéomique.
PCT/MY2012/000044 2011-03-30 2012-03-07 Procédé d'extraction de protéines à partir d'un matériau végétal riche en pectine Ceased WO2012134262A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
MYPI2011700031A MY165920A (en) 2011-03-30 2011-03-30 A protein extraction kit from plant material
MYPI2011700031 2011-03-30

Publications (2)

Publication Number Publication Date
WO2012134262A2 WO2012134262A2 (fr) 2012-10-04
WO2012134262A3 true WO2012134262A3 (fr) 2012-12-27

Family

ID=46932197

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/MY2012/000044 Ceased WO2012134262A2 (fr) 2011-03-30 2012-03-07 Procédé d'extraction de protéines à partir d'un matériau végétal riche en pectine

Country Status (2)

Country Link
MY (1) MY165920A (fr)
WO (1) WO2012134262A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104020296A (zh) * 2014-06-09 2014-09-03 无锡福阳生物科技有限公司 转基因蛋白cp4epsps的定量检测方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20220002997A (ko) * 2019-04-25 2022-01-07 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 식물 메신저 팩과 관련된 조성물 및 방법
CN111592586B (zh) * 2019-11-24 2021-12-03 杭州市农业科学研究院 一种适用于质谱鉴定的草莓雌蕊蛋白提取方法
CN111171108B (zh) * 2019-12-27 2021-12-10 中国热带农业科学院南亚热带作物研究所 一种澳洲坚果叶片总蛋白质的高效提取方法
CN112641101A (zh) * 2020-12-24 2021-04-13 常州市芙丽佳生物科技有限公司 包含橄榄仁提取物的蛋白粉及其制备方法
CN112617198A (zh) * 2020-12-24 2021-04-09 常州市芙丽佳生物科技有限公司 一种橄榄仁提取物的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830738A (en) * 1996-06-04 1998-11-03 Clemson University Extraction of pigment from plant material
US6313273B1 (en) * 1999-08-25 2001-11-06 Abbott Laboratories Soy proteins and methods for their production

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5830738A (en) * 1996-06-04 1998-11-03 Clemson University Extraction of pigment from plant material
US6313273B1 (en) * 1999-08-25 2001-11-06 Abbott Laboratories Soy proteins and methods for their production

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JUNG ET AL.: "Functionality of soy protein produced by enzyme-assisted extraction", JOURNAL OF THE AMERICAN OIL CHEMISTS' SOCIETY, vol. 83, no. 1, January 2006 (2006-01-01), pages 71 - 78 *
ZHOU ET AL.: "FT-IR methodology for quality control of arabinogalactan protein (AGP) extracted from green tea (Camellia sinensis)", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 57, 20 May 2009 (2009-05-20), pages 5121 - 5128 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104020296A (zh) * 2014-06-09 2014-09-03 无锡福阳生物科技有限公司 转基因蛋白cp4epsps的定量检测方法

Also Published As

Publication number Publication date
WO2012134262A2 (fr) 2012-10-04
MY165920A (en) 2018-05-18

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