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WO2011050050A3 - Use of a high-resolution melting assay to measure genetic diversity - Google Patents

Use of a high-resolution melting assay to measure genetic diversity Download PDF

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Publication number
WO2011050050A3
WO2011050050A3 PCT/US2010/053358 US2010053358W WO2011050050A3 WO 2011050050 A3 WO2011050050 A3 WO 2011050050A3 US 2010053358 W US2010053358 W US 2010053358W WO 2011050050 A3 WO2011050050 A3 WO 2011050050A3
Authority
WO
WIPO (PCT)
Prior art keywords
genetic diversity
resolution melting
melting assay
measure genetic
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/053358
Other languages
French (fr)
Other versions
WO2011050050A2 (en
Inventor
Susan Henrietta Eshleman
William Ian Towler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Publication of WO2011050050A2 publication Critical patent/WO2011050050A2/en
Publication of WO2011050050A3 publication Critical patent/WO2011050050A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)

Abstract

A method is provided for determining the level of genetic diversity in a sample using a high-resolution melting assay.
PCT/US2010/053358 2009-10-20 2010-10-20 Use of a high-resolution melting assay to measure genetic diversity Ceased WO2011050050A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US25332909P 2009-10-20 2009-10-20
US61/253,329 2009-10-20
US31423510P 2010-03-16 2010-03-16
US61/314,235 2010-03-16

Publications (2)

Publication Number Publication Date
WO2011050050A2 WO2011050050A2 (en) 2011-04-28
WO2011050050A3 true WO2011050050A3 (en) 2011-11-03

Family

ID=43900929

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/053358 Ceased WO2011050050A2 (en) 2009-10-20 2010-10-20 Use of a high-resolution melting assay to measure genetic diversity

Country Status (1)

Country Link
WO (1) WO2011050050A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014145243A2 (en) * 2013-03-15 2014-09-18 Biogen Idec Ma Inc. High resolution melting analysis assay for the detection of viral dna
WO2015164517A1 (en) * 2014-04-22 2015-10-29 The Johns Hopkins University Trained melt profile analysis methods and systems for reliable genotyping of sequence variants
CN106319092B (en) * 2016-09-27 2018-03-09 广州维佰生物科技有限公司 A kind of quick discriminating Porcine epidemic diarrhea virus classics strain and HRM detection primers, kit and the method for variant

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090155791A1 (en) * 2007-07-19 2009-06-18 Aarhus Universitet Method for detecting methylation status by using methylation-independent primers
US20090222503A1 (en) * 2005-09-20 2009-09-03 Robert Andrew Palais Melting Curve Analysis with Exponential Background Subtraction

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090222503A1 (en) * 2005-09-20 2009-09-03 Robert Andrew Palais Melting Curve Analysis with Exponential Background Subtraction
US20090155791A1 (en) * 2007-07-19 2009-06-18 Aarhus Universitet Method for detecting methylation status by using methylation-independent primers

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ETSUKO TAJIRI-UTAGAWA ET AL.: "Development of a Rapid High-Throughput Method for High-Resolution Melting Analysis for Routine Detection and Genotyping of Noroviruses", J. OF CLIN. MICROBIOL., vol. 47, no. 2, 10 December 2008 (2008-12-10), pages 435 - 440 *
IVAN SABOLA ET AL.: "A new approach for the evaluation of the human papillomavirus type 16 variability with high resolution melting analysis", JOURNAL OF VIROLOGICAL METHODS, vol. 162, 5 August 2009 (2009-08-05), pages 142 - 147, XP026682331, DOI: doi:10.1016/j.jviromet.2009.07.029 *
KYLIE HEWSON ET AL.: "Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model", ARCH VIROL., vol. 154, no. 4, 20 March 2009 (2009-03-20), pages 649 - 660, XP019722918 *
WILLIAM I. TOWLER ET AL.: "Analysis of HIV Diversity Using a High-Resolution Melting Assay", HRM ASSAY FOR DIVERSITY, vol. 26, no. 8, 31 August 2010 (2010-08-31), pages 913 - 918 *

Also Published As

Publication number Publication date
WO2011050050A2 (en) 2011-04-28

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