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WO2010133776A3 - Dispositif et procede d'isolement de cibles biologiques ou chimiques - Google Patents

Dispositif et procede d'isolement de cibles biologiques ou chimiques Download PDF

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Publication number
WO2010133776A3
WO2010133776A3 PCT/FR2010/000374 FR2010000374W WO2010133776A3 WO 2010133776 A3 WO2010133776 A3 WO 2010133776A3 FR 2010000374 W FR2010000374 W FR 2010000374W WO 2010133776 A3 WO2010133776 A3 WO 2010133776A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemical targets
biological
reaction chamber
relates
isolating biological
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2010/000374
Other languages
English (en)
Other versions
WO2010133776A2 (fr
Inventor
Guillaume Durin
Sylvaine Chabaud
Cyril Delattre
Dorothée JARY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to US13/321,177 priority Critical patent/US8703434B2/en
Priority to CN2010800272608A priority patent/CN102498402A/zh
Priority to EP10727008A priority patent/EP2433130A2/fr
Publication of WO2010133776A2 publication Critical patent/WO2010133776A2/fr
Publication of WO2010133776A3 publication Critical patent/WO2010133776A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

La présente invention se rapporte à un dispositif de collecte et de concentration de cibles biologiques ou chimiques en vue de leur détection; le dispositif comprenant une chambre réactionnelle (1) dans laquelle sont placées des billes magnétiques fonctionnalisées (2), un système d'agitation par ultrasons (3), un champ magnétique (4) pouvant être activé (position ON) ou inactivé (position OFF) et un système de déplacement de fluide (5) pour l'introduction (51) et l'évacuation (52) de tout ou partie du contenu de la chambre réactionnelle. La présente invention se rapporte également à un procédé de capture de cibles biologiques ou chimiques mettant en œuvre ledit dispositif.
PCT/FR2010/000374 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques Ceased WO2010133776A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US13/321,177 US8703434B2 (en) 2009-05-19 2010-05-17 Device and method for isolating biological or chemical targets
CN2010800272608A CN102498402A (zh) 2009-05-19 2010-05-17 用于分离生物或化学靶标的装置和方法
EP10727008A EP2433130A2 (fr) 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0902383A FR2945819B1 (fr) 2009-05-19 2009-05-19 Dispositif et procede d'isolement de cibles biologiques ou chimiques
FR0902383 2009-05-19

Publications (2)

Publication Number Publication Date
WO2010133776A2 WO2010133776A2 (fr) 2010-11-25
WO2010133776A3 true WO2010133776A3 (fr) 2011-05-19

Family

ID=41435283

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2010/000374 Ceased WO2010133776A2 (fr) 2009-05-19 2010-05-17 Dispositif et procede d'isolement de cibles biologiques ou chimiques

Country Status (5)

Country Link
US (1) US8703434B2 (fr)
EP (1) EP2433130A2 (fr)
CN (1) CN102498402A (fr)
FR (1) FR2945819B1 (fr)
WO (1) WO2010133776A2 (fr)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8999732B2 (en) * 2006-06-21 2015-04-07 Spinomix, S.A. Method for manipulating magnetic particles in a liquid medium
FR2993281B1 (fr) * 2012-07-13 2014-07-18 Biomerieux Sa Systeme automatise de lyse de microorganismes presents dans un echantillon, d'extraction et de purification des acides nucleiques desdits microorganismes aux fins d'analyse
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN102994383A (zh) * 2012-11-13 2013-03-27 陕西师范大学 磁-声复合作用的离体细胞标记装置和方法
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3567116A1 (fr) 2012-12-14 2019-11-13 10X Genomics, Inc. Procédés et systèmes de traitement de polynucléotides
BR112015019159A2 (pt) 2013-02-08 2017-07-18 10X Genomics Inc geração de código de barras de polinucleotídeos
US11029310B2 (en) * 2013-03-14 2021-06-08 Wisconsin Alumni Research Foundation Device and method for extracting a targeted fraction from a sample
US10040062B2 (en) * 2014-01-14 2018-08-07 Wisconsin Alumni Research Foundation Device and method for transferring a target between locations
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
JP2017522866A (ja) 2014-06-26 2017-08-17 １０エックス ジェノミクス， インコーポレイテッド 核酸配列の分析
US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
US10697000B2 (en) * 2015-02-24 2020-06-30 10X Genomics, Inc. Partition processing methods and systems
CN115651972A (zh) 2015-02-24 2023-01-31 10X 基因组学有限公司 用于靶向核酸序列覆盖的方法
CN115369161A (zh) 2015-12-04 2022-11-22 10X 基因组学有限公司 用于核酸分析的方法和组合物
WO2017197343A2 (fr) 2016-05-12 2017-11-16 10X Genomics, Inc. Filtres microfluidiques sur puce
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
CN106111329B (zh) * 2016-08-16 2017-10-13 闫维新 一种微型磁珠的操控装置
ES2951666T3 (es) * 2016-09-12 2023-10-24 Hoffmann La Roche Procedimientos y composiciones para purificar ácidos nucleicos bicatenarios
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP4029939B1 (fr) 2017-01-30 2023-06-28 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
SG11201901822QA (en) 2017-05-26 2019-03-28 10X Genomics Inc Single cell analysis of transposase accessible chromatin
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3954782A1 (fr) 2017-11-15 2022-02-16 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
AU2018353924A1 (en) 2017-12-29 2019-07-18 Clear Labs, Inc. Automated priming and library loading device
WO2019169347A1 (fr) 2018-03-02 2019-09-06 10X Genomics, Inc. Systèmes et appareils pour maintenir des plaques
EP3775271B1 (fr) 2018-04-06 2025-03-12 10X Genomics, Inc. Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques
CN109395875B (zh) * 2018-12-11 2023-10-20 苏州英赛斯智能科技有限公司 一种磁珠分离机构、装置以及磁珠分离方法
CN110208519A (zh) * 2019-06-27 2019-09-06 深圳华迈兴微医疗科技有限公司 一种主动液流控制微流控检测系统
CN110208520A (zh) * 2019-06-27 2019-09-06 深圳华迈兴微医疗科技有限公司 一种主动液流控制微流控检测系统
US11857981B2 (en) 2019-12-23 2024-01-02 10X Genomics, Inc. Magnetic separator for an automated single cell sequencing system
US11701668B1 (en) 2020-05-08 2023-07-18 10X Genomics, Inc. Methods and devices for magnetic separation
US11946038B1 (en) 2020-05-29 2024-04-02 10X Genomics, Inc. Methods and systems including flow and magnetic modules
US12263482B1 (en) 2020-06-03 2025-04-01 10X Genomics, Inc. Methods and devices for magnetic separation in a flow path
CN113881663A (zh) * 2021-10-25 2022-01-04 苏州缔因安生物科技有限公司 一种基于超声波的核酸提取方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004053115A1 (fr) * 2002-12-09 2004-06-24 Tsinghua University Separation cellulaire rapide a base de magnetisme
US20050013741A1 (en) * 2001-11-19 2005-01-20 A' Brassard Lothar Device and method for treating magnetic particles
US20060252031A1 (en) * 2004-07-23 2006-11-09 Platypus Technologies, Llc Bead based assays using a liquid crystal reporter
WO2007097740A2 (fr) * 2005-02-11 2007-08-30 Massachusetts Institute Of Technology Méthodes et appareils de préparation d'échantillons
WO2008131554A1 (fr) * 2007-04-26 2008-11-06 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Health Procédé destiné à isoler des microorganismes à partir d'échantillons et système, appareil et compositions à cet effet

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4628037A (en) 1983-05-12 1986-12-09 Advanced Magnetics, Inc. Binding assays employing magnetic particles
US6159378A (en) 1999-02-23 2000-12-12 Battelle Memorial Institute Apparatus and method for handling magnetic particles in a fluid
GB0001450D0 (en) 2000-01-21 2000-03-08 Genpoint As Cell isolation method
JP2003097970A (ja) 2001-07-19 2003-04-03 Alps Electric Co Ltd 回転型センサ

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050013741A1 (en) * 2001-11-19 2005-01-20 A' Brassard Lothar Device and method for treating magnetic particles
WO2004053115A1 (fr) * 2002-12-09 2004-06-24 Tsinghua University Separation cellulaire rapide a base de magnetisme
US20060141450A1 (en) * 2002-12-09 2006-06-29 Xu Zhang Magnetism based rapid cell separation
US20060252031A1 (en) * 2004-07-23 2006-11-09 Platypus Technologies, Llc Bead based assays using a liquid crystal reporter
WO2007097740A2 (fr) * 2005-02-11 2007-08-30 Massachusetts Institute Of Technology Méthodes et appareils de préparation d'échantillons
WO2008131554A1 (fr) * 2007-04-26 2008-11-06 Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of Health Procédé destiné à isoler des microorganismes à partir d'échantillons et système, appareil et compositions à cet effet

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ANONYME: "PATHATRIX Overvieuw", MATRIX MICROSCIENCE PRODUCT DETAILS, 3 May 2008 (2008-05-03), XP002563982, Retrieved from the Internet <URL:http://www.matrixmsci.com/pathatrix.htm> [retrieved on 20100119] *

Also Published As

Publication number Publication date
US8703434B2 (en) 2014-04-22
WO2010133776A2 (fr) 2010-11-25
FR2945819B1 (fr) 2011-06-17
US20120190037A1 (en) 2012-07-26
FR2945819A1 (fr) 2010-11-26
CN102498402A (zh) 2012-06-13
EP2433130A2 (fr) 2012-03-28

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