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WO2010115074A1 - Procédés de purification pour composés bio-organiques - Google Patents

Procédés de purification pour composés bio-organiques Download PDF

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Publication number
WO2010115074A1
WO2010115074A1 PCT/US2010/029740 US2010029740W WO2010115074A1 WO 2010115074 A1 WO2010115074 A1 WO 2010115074A1 US 2010029740 W US2010029740 W US 2010029740W WO 2010115074 A1 WO2010115074 A1 WO 2010115074A1
Authority
WO
WIPO (PCT)
Prior art keywords
composition
bio
organic
host cells
crude
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2010/029740
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English (en)
Other versions
WO2010115074A8 (fr
Inventor
Pinar Tabur
Glenn Dorin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amyris Inc
Original Assignee
Amyris Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris Biotechnologies Inc filed Critical Amyris Biotechnologies Inc
Publication of WO2010115074A1 publication Critical patent/WO2010115074A1/fr
Publication of WO2010115074A8 publication Critical patent/WO2010115074A8/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C7/00—Purification; Separation; Use of additives
    • C07C7/10—Purification; Separation; Use of additives by extraction, i.e. purification or separation of liquid hydrocarbons with the aid of liquids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes

Definitions

  • Figure 6 is a plot of total acid number (TAN) as a function of time for a crude bio-organic composition that was obtained by liquid/liquid separation at an acidic pH (produced by farnesene producing yeast strain B).
  • the amount of calcium hydroxide used was 0.356 % by weight.
  • the clarifying step occurs by sedimentation followed by decantation. In still other embodiments, the clarifying step occurs by filtration. In certain embodiments, the clarifying step occurs by centrifugation. In certain other embodiments, the clarifying step occurs in a continuous disk stack nozzle centrifuge.
  • FIG. 2 shows the separation disc covered with emulsion. When enough emulsion forms, then the centrifuge becomes ineffective in separating the bio-organic compound from the aqueous phase.
  • a concentrated bio-organic composition was obtained as described by
  • This example describes a dose response of the total acid number as a function of the amount of calcium hydroxide of the crude bio-organic composition derived from yeast strain B.
  • TFF Process tangential flow filtration
  • approximately 200 liters of clarified broth was used.
  • the clarified broth was processed in series through 4 Industrial TFF cartridges (316L Stainless Steel, 1.5" diameter tube, 0.1 micrometer pore size, 0.35m 2 each).
  • the purpose of the TFF processing is to remove water and aqueous components, leaving behind a concentrated clarified broth.
  • the initial processing conditions for the TFF were l ⁇ psig inlet pressure, 5psig outlet pressure, and 880mL/min of permeate flux (38L/m 2 /hr). Time course samples of permeate and retentate streams were obtained. Throughout the course of processing, the permeate flux gradually dropped off due to accumulation of solids in the cartridges and increased viscosity of the retentate.
  • Approximately 14 liters of concentrated clarified broth was recovered from the

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Water Supply & Treatment (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Treatment Of Water By Oxidation Or Reduction (AREA)

Abstract

La présente invention concerne des procédés et des systèmes de purification de composés bio-organiques. Dans certains modes de réalisation, ce procédé de purification comprend une étape consistant à rendre neutre le pH de la composition. Dans d'autres modes de réalisation, le procédé de purification comprend, éventuellement, une étape de neutralisation de la composition visant à ramener l'indice d'acide à moins d'environ 0,5 mg KOH/g de composé bio-organique.
PCT/US2010/029740 2009-04-02 2010-04-02 Procédés de purification pour composés bio-organiques Ceased WO2010115074A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16616709P 2009-04-02 2009-04-02
US61/166,167 2009-04-02

Publications (2)

Publication Number Publication Date
WO2010115074A1 true WO2010115074A1 (fr) 2010-10-07
WO2010115074A8 WO2010115074A8 (fr) 2011-07-14

Family

ID=42358697

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2010/029740 Ceased WO2010115074A1 (fr) 2009-04-02 2010-04-02 Procédés de purification pour composés bio-organiques

Country Status (1)

Country Link
WO (1) WO2010115074A1 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019030073A1 (fr) * 2017-08-07 2019-02-14 Total Raffinage Chimie Procédé de récupération d'isoprénoïdes produits par des microorganismes
WO2020006251A1 (fr) * 2018-06-29 2020-01-02 Amyris, Inc. Procédés pour récupérer des composés isoprénoïdes non miscibles dans l'eau à partir d'une biomasse microbienne

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7179311B2 (en) 2003-01-31 2007-02-20 Chevron U.S.A. Inc. Stable olefinic, low sulfur diesel fuels
WO2007139924A2 (fr) 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Dispositif de fabrication de composés bio-organiques
WO2007140339A2 (fr) 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production d'isoprénoïdes
US7399323B2 (en) 2006-10-10 2008-07-15 Amyris Biotechnologies, Inc. Fuel compositions comprising farnesane and farnesane derivatives and method of making and using same
WO2008113041A2 (fr) 2007-03-14 2008-09-18 Ls9, Inc. Procédé de production d'hydrocarbures de bas poids moléculaire à partir de ressources renouvelables
WO2008133658A2 (fr) 2006-11-21 2008-11-06 Amyris Biotechnologies, Inc. Compositions de carburant pour aviation et leurs procédés de fabrication et d'utilisation
WO2008140492A2 (fr) 2006-11-21 2008-11-20 Amyris Biotechnologies, Inc. Compositions de carburéacteur et leurs procédés de fabrication et d'utilisation
WO2008151149A2 (fr) 2007-06-01 2008-12-11 Solazyme, Inc. Production d'huile dans des micro-organismes
WO2009014636A2 (fr) 2007-07-20 2009-01-29 Amyris Biotechnologies, Inc. Compositions de carburant comprenant du tétraméthylcyclohexane

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7179311B2 (en) 2003-01-31 2007-02-20 Chevron U.S.A. Inc. Stable olefinic, low sulfur diesel fuels
WO2007139924A2 (fr) 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Dispositif de fabrication de composés bio-organiques
WO2007140339A2 (fr) 2006-05-26 2007-12-06 Amyris Biotechnologies, Inc. Production d'isoprénoïdes
US20080274523A1 (en) 2006-05-26 2008-11-06 Neil Stephen Renninger Production of isoprenoids
US7399323B2 (en) 2006-10-10 2008-07-15 Amyris Biotechnologies, Inc. Fuel compositions comprising farnesane and farnesane derivatives and method of making and using same
WO2008133658A2 (fr) 2006-11-21 2008-11-06 Amyris Biotechnologies, Inc. Compositions de carburant pour aviation et leurs procédés de fabrication et d'utilisation
WO2008140492A2 (fr) 2006-11-21 2008-11-20 Amyris Biotechnologies, Inc. Compositions de carburéacteur et leurs procédés de fabrication et d'utilisation
WO2008113041A2 (fr) 2007-03-14 2008-09-18 Ls9, Inc. Procédé de production d'hydrocarbures de bas poids moléculaire à partir de ressources renouvelables
WO2008151149A2 (fr) 2007-06-01 2008-12-11 Solazyme, Inc. Production d'huile dans des micro-organismes
US20090047721A1 (en) 2007-06-01 2009-02-19 Solazyme, Inc. Renewable Diesel and Jet Fuel from Microbial Sources
WO2009014636A2 (fr) 2007-07-20 2009-01-29 Amyris Biotechnologies, Inc. Compositions de carburant comprenant du tétraméthylcyclohexane

Non-Patent Citations (5)

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Title
"Pollution Prevention Guidance Manual for the PFPR Industry", pages: 41 - 46
AMALVY ET AL., CHEM. COMMUN., 2003, pages 1826 - 182
GALLUP ET AL., ENERGY FUELS, vol. 21, no. 3, 2007, pages 1741 - 1759
GEORGE ALTHER, WATER ENGINEERING & MANAGEMENT, 2001, pages 27 - 29
RO DAE-KYUN ET AL: "Production of the antimalarial drug precursor artemisinic acid in engineered yeast", NATURE, NATURE PUBLISHING GROUP, LONDON, GB LNKD- DOI:10.1038/NATURE04640, vol. 440, 13 April 2006 (2006-04-13), pages 940 - 943, XP002510909, ISSN: 0028-0836 *

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019030073A1 (fr) * 2017-08-07 2019-02-14 Total Raffinage Chimie Procédé de récupération d'isoprénoïdes produits par des microorganismes
US11103808B2 (en) 2017-08-07 2021-08-31 Amyris, Inc. Process for recovering isoprenoids produced by microorganisms
WO2020006251A1 (fr) * 2018-06-29 2020-01-02 Amyris, Inc. Procédés pour récupérer des composés isoprénoïdes non miscibles dans l'eau à partir d'une biomasse microbienne
CN112771169A (zh) * 2018-06-29 2021-05-07 阿迈瑞斯公司 从微生物生物质回收水不混溶性类异戊二烯化合物的方法
JP2021529512A (ja) * 2018-06-29 2021-11-04 アミリス， インコーポレイテッド 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法
US11312976B2 (en) 2018-06-29 2022-04-26 Amyris, Inc. Methods for recovering water-immiscible isoprenoid compounds from microbial biomass
JP7504031B2 (ja) 2018-06-29 2024-06-21 アミリス， インコーポレイテッド 微生物バイオマスから不水溶性イソプレノイド化合物を回収するための方法
CN112771169B (zh) * 2018-06-29 2024-07-09 阿迈瑞斯公司 从微生物生物质回收水不混溶性类异戊二烯化合物的方法
US12031169B2 (en) 2018-06-29 2024-07-09 Amyris, Inc. Compounds, compositions, and methods for recovering water-immiscible compounds from microbial biomass

Also Published As

Publication number Publication date
WO2010115074A8 (fr) 2011-07-14

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