WO2010106468A1

WO2010106468A1 - Dry powdery fodder additive, supplement or fodder containing algae spirulina platensis

Info

Publication number: WO2010106468A1
Application number: PCT/IB2010/051039
Authority: WO
Inventors: Vytas RIMKUS; Almantas Simkus; Raimundas Syvys; Sarunas Birutis
Original assignee: SPILA UAB
Current assignee: SPILA UAB
Priority date: 2009-03-16
Filing date: 2010-03-10
Publication date: 2010-09-23
Anticipated expiration: 2011-09-16
Also published as: LT5613B; LT2009020A

Abstract

The invention claimed relates to fodder production, to produce particular fodder additive, supplement or fodder with algae Spirulina platensis. Biomass of algae Spirulina platensis both in dry and preserved with molasses form is stable under granulat ion and is admixed to farinaceous fodder. The preparation obtained has probiotic and hemopoietic activity, improves digestability of fodder s nutrients and positively affects livestock health, reproduction and secretion of milk. The dose is up to 300 g per day per livestock animal.

Description

Title of Invention: DRY POWDERY FODDER ADDITIVE, SUPPLEMENT OR FODDER CONTAINING ALGAE

SPIRULINA PLATENSIS

Technical Field

[1]

Background Art

[2] Nowadays there is a widespread and rapidly increasing interest in the natural and ecological preparations intended to improve the digestibility and absorption of forage and having probiotic as well as immunomodulatory qualities. All preparations may be classified into probiotics, prebiotics and phytobiotics. They are among the most promising, relatively new and rapidly emerging natural and ecological preparations produced by biotechnology methods and intended to improve the microbial balance of the digestive tract, animal health, and quality of production ( Hamidreza and Werner, 2005; TpaΛOBa H jφ., 2001).

[3] Phytobiotics include preparations of plant origin and their phytochemical ingredients.

Some of phytochemical materials are known to bind cholesterol, reduce its resorption and effectively remove it from the organism, have secretomotoric and secretolytic properties, improve immune system of animals, eliminate pathogenic microflora, and reduce concentration of ammonia and hydrogen sulfide in the organism of animals.

[4] Algae also belong to the group of phytobiotics. The cyanobacteria Spirulina platens is besides being a rich source of vitamins, amino acids, phytohormones, ferments, and microelements, also helps to increase their absorption from forage. In this way the organism is provided with phytochemical materials and the amount of unabsorbed feed is reduced (Goksan et al., 2007; Patil et al., 2008; EropoBa H ,zφ., 2006; IleτpaκθB, 2007).

[5] The additives and supplements are prepared so as to make them dry and powdery, easy to include into fodder and easily used by foragers and cattle-breeders. Disclosure of Invention Technical Problem

[6] The aim of the envisaged invention is to create a dry, powdery fodder additive which could be used in forage and granulated fodder, and a have positive effect on animal physiological processes and productivity as well as increase the quantity and quality of the economically useful animals' production. Mode for Invention

[7] The stated goal is achieved when the animal diet involves a fodder additive which comprises natural cyanobacteria Spirulina platensis containing, on the dry matter basis, not less than 12,5 % of the blue pigment phycocyanin, preserved molasses and animal fodder(bran, milled grains or a blend of milled grains). The algae is preserved with molasses at the following ratio of components (% by mass): 13-40 percent cyanobacteria Spirulina platensis biomass, the rest is molasses. Before preservation the cyanobacteria Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture. The molasses is heated up to 36 - 42 ⁰C temperature. The vacuumed cyanobacteria Spirulina platensis biomass is poured while mixing into the heated molasses. The mass is heated at the temperature of 36-42°C for 20 - 35 minutes while mixing. The cyanobacteria S pirulina platensis biomass preserved with molasses remains active for up to 12 months.

[8] The obtained cyanobacteria Spirulina platensis biomass preserved with molasses is left for at least 24 hours but not longer than 12 months. During this time the algae becomes saturated with molasses and its cell maintains integrity while pelleting. The molasses - cyanobacteria Spirulina platensis mass is equally sprayed into the animal fodder in the amount of 3 - 5 % and then it is granulated. In 50 - 225 g of the obtained fodder additive there is 1 g cyanobacteria Spirulina platensis biomass containing 70 - 75 % moisture. The obtained fodder additive does not group into layers and is dustless. In addition, it is easy to transport and store and can be easily utilized by cattle breeders. The obtained powdery fodder additive remains active for 6 months.

[9] The stated goal may be achieved by supplementing the animal diet with a fodder additive which comprises natural cyanobacteria Spirulina platensis biomass, containing not less than 12,5 % of the blue pigment phycocyanin on the dry matter basis , and dried in the drying oven until material air dry condition(9,5 - 14,0 % moisture), and animal fodder(bran, milled grains or a blend of milled grains.) Before drying, the cyanobacteria Spirulina platensis biomass is washed with drinking water and vacuumed to 70 - 75 % moisture content. The biomass is dried in the drying oven up to the temperature of 36-42 ⁰C. The dried cyanobacteria Spirulina platensis biomass remains active for 36 months.

[10] The air dry Spirulina platensis biomass is equally mixed into the animal fodder in the amount of 3 - 15%; the animal fodder may be granulated. There is 1 g of air dry Spirulina platensis biomass in 6,7-33,3 g of the obtained fodder additive. The obtained fodder additive is not dusty, does not group into layers, is easy to store and utilize. The obtained powdery fodder additive remains active for 24 months.

[11] The animal diet, complemented with a mixture of Spirulina platensis biomass and animal fodder, stimulates the vital physiological processes in the organisms of economically useful animals, improves microbial balance of the digestive tract, digestibility of nutrients, and promotes health. As a result the productivity of eco- nomically useful animals increases by 7- 19 %. The allowance of this dry powdery fodder additive for one animal is 2-300 g a day.

[12] In order to illustrate the invention the granulated Spirulina Platensis preparation was used in feeding experiment with dairy cows and sows. The preparation used in the experiment with dairy cows contained 1 g of air dried Spirulina platensis material per 10 g. The dairy cows were fed 20 g per head per day of this preparation. The preparation used in the experiment with sows contained 1 g of air dried Spirulina platensis preserved with molasses per 100 g of the pelleted fodder . The sows were fed 200 g per head per day of this preparation.

[13] THE EXPERIMENT WITH DAIRY COWS

[14] The experiment was carried out with Lithuanian Black-and- White cows. Taking into account their age, lactation and productivity, two groups of cows were formed: control and experimental. Each of the group included 10 cows of III-IV lactation. The experiment was conducted with cows from 70 to 130 lactation day. During the indoor period the animals of both groups were kept and fed under the same conditions, except that cows of the experimental group together with fodder were individually given 20 g of the granulated Spirulina platensis preparation containing 2 g of Spirulina platensis biomass. At the beginning of the experiment and then every 15 days the control milkings of the cows were carried out. The milk samples were analyzed by routine research methods of Vl 'Pieno tyrimai '. The fat, proteins, lactose and urea was determined by the mid-infrared spectroscopic method (LST ISO 9622), while the somatic cells count was determined by fluoro- optoelectronic method (LST EN ISO 13366-3).

[15] In order to evaluate the physiological state of the cows morphological and biochemical blood parameters were analyzed. The blood was sampled from the tail vein into the vacuum tubes (Venoject II, Terumo Europe, Belgium) 2 hours after morning feeding at the beginning of the experiment, after 1 month and then after 2 months, i. e. at the end of the experiment. For the morphological analysis the blood was collected into the tubes with anticoagulant EDTA (K2), while the blood for the biochemical analysis was taken into the tubes without anticoagulants. The amount of hemoglobin was determined by the colorimetric method. The calculation of erythrocytes and leucocytes was performed in Goriajev's chamber. The glucose was determined by the reflex photometer Accutrend GCT 2001 (Germany), the total protein was determined by refractometer RL3 (Poland).

[16] The rumen contents were collected from the caudo- ventral part of the rumen using throat-esophagus tube GDZ-I at the beginning of the experiment and every 20 days, 3 hours after morning feeding. The analysis of the following indicators of the rumen contents was performed: pH was measured by electrometric method using pH-meter CP-315; the reduction activity of bacteria was evaluated by G. Dirksen's method; the reaction of glucose fermentation was performed by Einhorn sacharometer according to the methodology described by J. Bakϋnas; the number of infusoria was determined using Fux-Rosenthal chamber according to the method of J. A. Schultz; the total volatile fatty acids (VFA) was determined by distilling the rumen contents using Markham apparatus according to V.V. Ciupko and M. V. Berus method. The digestibility of the organic matter (OM) of the grass forage was analyzed by I stage in vitro method.

[17] The analysis of cow rumen contents included the calculation of the total bacteria count (TBC), the lactate fermenting bacteria count (LFBC) and the cellulolytic bacteria count (CBC) according to N. O. van Gylswyk's technique for cultivating obligate anaerobes.

[18] During the analysis of the rumen contents the amount of anaerobic and facultative bacteria was determined as well as lactobacillus, streptococci and yeasts by applying the plate method. In order to determine the amount of aerobic and facultative anaerobic bacteria, the nutrient agar was used; the amount of lactobacillus was determined using MRS agar, streptococci were analyzed employing Slanetz-Bartley agar, while in the analysis of yeasts Sabouraud agar was used (Liofilchem, Italy).

[19] The data of the analysis were processed by the statistical analysis method using statistic package 'R 2.2.0 '. The statistical analysis was performed using Student's t-test. The results were considered reliable when p<0.05.

[20] The results of cows' control milkings and qualitative indicators of milk composition which were obtained during the experiment are presented in Table 1. As shown by the results of the analysis, the Spirulina platensis preparation had an effect upon the milk yield of the dairy cows after the first 15 days of the experiment. The milk production of the cows of the control group remained almost the same during this period, whereas the control group cows produced 0,9 kg or 3,84 percent more milk than at the beginning of the experiment. After 30 days of the experiment the cow of the experimental group on average yielded 1,58 kg or 7,48 percent more milk per day than the cow from the control group. After 45 days of the experiment the cow of the experimental group on average yielded 1,64 kg or 7,0 percent more milk per day than the cow from the control group. At the end of the experiment, after 60 days of the trial the cow of the experimental group on average yielded 3,33 kg or 14,4 percent more milk per day than that of the control group. Comparing the milk yield of this period with that of the beginning of the experiment, the milk yield of the experimental group was by 2,96 kg or 12,62 percent higher per day, whereas the milk yield of the cows of the control group was by 1,54 kg or 6,7 percent lower.

[21] As the results of the analysis indicate, the preparation containing cyanobacteria

Spirulina platensis affects the composition of milk. During the trial the content of milk fat in the milk of the control group cows remained almost the same, whereas in the milk of the cows of the experimental group it increased by 0,14 percent. The protein content of the milk in the control group cows decreased by 0,19 percent, whereas in the milk of the experimental cows it increased by 0,13 percent.

[22] At the end of the trial the somatic cells count in the control cows was by 66,75 thousand/ml or 57,5 percent higher than in the milk of the cows of the experimental group. This demonstrates that a cyanobacterium has a positive effect on the quality of milk and the health of cows' udder.

[23]

[Table 1]

[Table ]

Table 1. Indicators of control milkings and milk composition

[24] On the basis of the results presented in Table 1, it can be concluded that the preparation containing cyanobacteria Spirulina platensis stimulates the secretion of milk glands; as a result the milk yield can increase by 14,4 percent per day. During 60 days of this experiment the cows of the experimental group on average produced 92, 1 kg more milk, 5,49 kg more milk fat, 4,43 kg more milk protein, and 3,53 kg more milk sugar in comparison to the cows of the control group.

[25] The analysis of the rumen contents showed that during each stage of the experiment the biochemical indicators of the rumen in cows were within physiological norms. The results of the rumen analysis are shown in Table 2. During the analysis of the indicators of the rumen contents it was determined that after one month of the experiment the amount of volatile fatty acids (VFA) increased (p<0,05) by 32,9 percent and after two month by 9,4 percent in cows which were fed cyanobacteria Spirulina platensis additive. Volatile fatty acids are the main source of energy for cows. We assume that the animals of the experimental group received more energy necessary for milk synthesis.

[26] [Table 2]

[Table ]

Table 2. Biochemical and microbiological parameters of rumen contents

[27] The glucose fermentation experiment (Table 2) showed that the fermentation processes in the rumen of the experimental cows were by 24 percent more active than in the control animals.

[28] The rumen of cattle is populated with anaerobic bacteria, infusoria and fungi. They quickly colonize the fodder which gets into the rumen. Due to the active participation of microorganisms, the organism receives 70 percent more energetic matter which is necessary to maintain vital physiological processes and yield production. Infusoria play an important role in physiological processes of the rumen and are involved in all processes of metabolism. Due to their abundance and mobility they form a microcirculation, loosen and mix the rumen contents. As a result the surface area of the fodder increases and the optimal conditions for bacteria ferments are created. Under the effect of the preparation containing cyanobacteria Spirulina platensis, the rumen contents of the experimental cows after the first month of the experiment contained 37,55 thousand/ml or 37,75 percent (p<0,05) more infusoria in comparison to that of the control cows and after the second month 15,43 thousand/ml or 11,24 percent more than in the rumen contents of the control group cows. The microorganisms in the rumen contents of cows not only decompose fodder, but also synthesize their body proteins from the materials in the rumen. Later as they move through the digestive tract they are digested by the ferments and used by the animal organism. The proteins of microorganisms, in comparison to the proteins of fodder, are much closer to the organism of cows. The total increase of microorganisms in rumen is very significant. The analysis of the digestibility of the organic matter of fodder during which the analyzed fodder was incubated with rumen contents of the cows showed that the digestibility of the organic matter of hay, haylage and silage in experimental cows was respectively 7,4 percent (p<0,05), 2,6 percent and 5,6 percent (p<0,05) better than that of the cows of the control group. This explains the improved digestibility of nutrients of ration under the influence of cyanobacteria Spirulina platensis.

[29] Anaerobic microorganisms in the rumen actively participate in the digestibility of the organic matter. The analysis of the rumen contents showed that the average total anaerobic bacteria count increased in the experimental cows which diet was complemented with cyanobacteria Spirulina platensis. After 1 month of the experiment the TBC was by 6,3 percent higher (p<0,01) in the rumen of cows of the experimental group and after 2 months this number was by 8,6 percent higher (p<0,001) than in the rumen of cows of the control group. There was no much change in the average number of lactate fermenting bacteria and cellulolytic bacteria in the rumen of experimental cows.

[30] The preparation containing cyanobacteria Spirulina platensis which was used in the experiment had an influence on the amount of aerobic bacteria and microaerophilic bacteria - lactobacilli and facultative anaerobes. The total anaerobic and facultative anaerobic bacteria count in the rumen contents of the experimental group cows was by 5,2 percent higher than in the rumen contents of the control group cows. Furthermore, after one month of the experiment the amount of microaerophilic bacteria - lactobacilli in the rumen of the experimental cows was by 6,9 percent higher (p<0,001) than in that of the control cows, while after the second month it was by 10,6 percent higher than (p<0,001) in the rumen of the control group cows. At the end of the experiment the amount of other bacteria producing lactic acid, i.e. streptococci was by 12,2 percent (p<0,001) higher in the rumen of the experimental group than in that of the control group cows. The amount of yeasts in the rumen of the cows of the control group was by 6,8 higher compared to that of the control group cows.

[31] The obtained results of the rumen microbiological analysis showed that Spirulina platensis has probiotic activity and increases the concentration of beneficial microorganisms in the cows' rumen.

[32] The hematologic analysis demonstrated that the blood parameters of the cows of both groups corresponded to the physiological norms; they were sufficiently high and indicated good state of health of the cows. After the first month of the experiment the amount of erythrocytes in the blood of the cows of both groups was almost equal; however, at the end of the experiment the amount of hemoglobin and erythrocytes in the experimental cows' blood, in comparison to the control cows, was by 12,5 percent (p<0,05) and 6,8 percent higher, respectively. This indicates that the oxidation processes were more active in the organism of the cows of the experimental group. Based on the obtained results it was concluded that the preparation has an effect upon hemopoiesis.

[33] Conclusions:

[34] 1. 1. The air dry cyanobacteria Spirulina plate snis biomass is resistant to granulation process, positively affects the cows' state of health and stimulates the secretion of milk glands in the dairy cows. As a result the dairy cows produce a greater amount of high quality yield.

[35] 1. 2. The cyanobacteria Spirulina platensis fodder additive has probiotic activity, improves the microbial balance, the digestibility of feed nutrients, stimulates fermentation processes and has hemopoietic properties.

[36] THE EXPERIMENT WITH SOWS

[37] Two analogous groups (control and experimental) of sows were formed both of which included 6 animals. The sows of Landrace breed were inseminated with semen of Duroc and Pietrain cross bred boars. The experiment with the sows was conducted from 82 day of pregnancy up to the weaning of piglets. The piglets were weaned at 28 day of age. All the pigs used in the trial were kept and fed under the same standardized conditions, except that the regular ration fed to the pigs of the experimental group was complemented with the granulated cyanobacteria Spirulina platensis preparation. Its daily allowance was 2 g of cyanobacteria biomass containing 75 percent moisture.

[38] The analysis of the influence of the Spirulina platensis preparation on the sows' reproduction and the growth performance of suckling pigs covered the following parameters: litter size (units), piglet's weight at birth (kg), litter weight of piglets at birth (kg), litter weight of piglets at 21 day of age (kg), piglet's weight at 21 day of age (kg), litter weight of piglets at weaning (kg), weight of piglets at weaning(kg), daily gain of piglets (g), the survival percentage of piglets. Based on the weighting data the daily gain of the piglets was calculated. [39] The sows' milk was sampled 4 hours and then 14 days after farrowing. The analysis of the milk samples were conducted in the accredited laboratory Vl 'Pieno tyrimai' according to the approved methods. The fat, protein and lactose content was determined by mid-infrared spectroscopic method (LST ISO 9622) using 'LactoScope FTIR ' equipment (FT 1.0. 2001; Delta Instruments, Netherlands). The relative milk yielding capacity of the sows was determined according to the litter weight on 21 day after farrowing.

[40] At the end of the experiment the digestibility analysis was conducted and blood samples were taken for hematological analysis. The digestibility of the nutrients of the sows' ration was determined using TiO₂ indicator according to its concentration in fodder and faeces. On the basis of the data of the fodder and faeces chemical analysis, the digestibility ratio was calculated. In the fodder and faeces the following was determined: total nitrogen content, protein and non-protein nitrogen - by Kjeldahl method; dry matter content was determined by drying at the temperature of 100-105 ° C, crude fat - by Sokslet method; crude fibre - by the method of Kurschner.

[41] The morphological parameters of the sows' blood were determined by SWELAB-

AC920 EO+ (2004; Boule Medical AB, Sweden) instrument, while the biochemical indicators were analyzed by semi-automatic analyzer Screen Master Plus (2002; Hospitex Diagnostics S. A., Italy).

[42] The obtained data were processed by the statistical analysis method using statistic package 'R 1.7.1. ' (http://www.r-project.org) and the WinExcel program. The reliability of arithmetic means (p) was determined by Student. The results were considered reliable when p<0,05.

[43] The reproductive data of sows are presented in Table 1. As the farrowing data show, the litter size of the sows of the experimental group was 1,83 piglets smaller than that of the control group; however, the birth weight of the litter and the piglet delivered by the sows of the experimental group was by 0,25 kg or 1,53 percent and by 0,27 kg or 19,85 percent (p<0,05) higher, respectively, in comparison to those of the control sows.

[44] [Table 3]

[Table ]

Table 1. Reproductive indicators of sows (n = 6)

[45] On 21 day of the experiment the litter weight and milk yielding capacity of the sows of the experimental group was by 5,86 kg or 11,23 percent (p<0,05) higher than that of the control group sows. The average piglet's weight, the daily gain and the survival of the piglets of the experimental sows was by 0,89 kg or 17,12 percent (p< 0,05), 29 g or 15,85 percent, and 10,1 percent higher, respectively, compared to those of the control group sows.

[46] At the time of weaning, i.e. on 28 day after farrowing, the litter weight, the piglet's weight and the daily gain of the piglets of the experimental sows was by 6,94 kg or 10,74 percent, 1,07 kg or 16,56 percent (p<0,05) and 29 g or 15,93 percent higher than those of the control group sows.

[47] The analysis of the sows' milk composition, which was carried out 4 hours and then 14 days after farrowing, showed (Table 2) that the microalgae Spirulina platensis additive had a positive effect on the parameters of the sows' milk composition. Four hours after farrowng, the milk of the experimental sows contained 0,33 percent more fat, 0,39 percent more protein (p<0,05), and 0,38 percent more lactose (p<0,05) than the milk of the sows of the control group.

[48] [Table 4] [Table ] Table 2. Chemical composition of sows' milk (n = 6)

[49] Fourteen days after farrowing (Table 2), the same patterns were present as those which were seen 4 hours after farrowing, i.e. the milk of the sows of the experimental group contained 0,32 percent more fat, 0,14 percent more protein and 0,13 percent more lactose compared to the milk of the sows of the control group.

[50] Based on the results of the analysis it can be concluded that under the influence of the preparation containing algae Spirulina platensis the sows synthesize more milk and of better nutritive value, while a greater amount of nutrients stimulates piglets' growth and increases their survival.

[51] The digestibility analysis of the nutrients of the ration (Table 3) showed that the Spirulina platensis preparation improve the digestibility of the nutrients of the experimental sows ration. The digestibility of the dry matter, protein, fat, and fibre in the experimental group sows was by 1,12 percent, 3,63 percent, 1,72 percent, and 1,33 percent higher, respectively, than the digestibility of the sows of the control group.

[52] [Table 5]

[Table ]

Table 3. The digestibility of nutrients of sows' ration (n = 6)

[53] The morphological and biochemical blood analysis (Table 4) showed that microalgae Spirulina platensis had a positive influence on the hematological indicators of the sows. The blood of the experimental sows contained 7,2 g/1 or 5,33 percent (p<0,05) more hemoglobin, 0,9 x 10¹²/l or 14,52 percent more erythrocytes, 0,2 x 10⁹/l leucocytes, and 0,2 percent more total protein than the blood of the sows of the control group. The alg&eSpirulina platensis had no effect on the calcium and phosphorus content in the blood.

[54] [Table 6] [Table ] Table 4. Hematological indicators of sows' blood (n = 6)

[55] *p < 0,05 [56] From the results of the analysis (Table 4) it can be seen, that the hematological indicators in the blood of both groups of sows are sufficiently high and indicate a good state of health of the pigs. Based on the obtained results it can be claimed that the vital processes in the organism of the experimental sows were much intensive than in that of the control sows. Therefore, it can be concluded that the algae Spirulina platensis preparations in the sows' ration had a positive influence on hemopoiesis. [57] Conclusions:

[58] 1. 1. The Spirulina platensis biomass preserved by molasses is resistant to the granulation process.

[59] 1. 2. The sows' daily ration complemented with preparation which contains 2 g of 75 percent moisture preserved Spirulina platensis biomass helped to increase the reproductive properties of the sows. The piglet's weight at birth increased by 19,86 percent (p<0,05) , milk yielding capacity by 11,23 percent, the piglet's weight on 21 and 28 day of age by 17,12 percent (p<0,05) and 16,56 percent (p<0,05), respectively, and the survival of the piglets increased by 10,1 percent.

[60] 1. 3. Under the influence of algae, the sows digested the nutrients of the ration better and synthesized more milk of better nutritive value.

[61] 1. A.Spirulina platensis intensified vital physiological processes and had an effect on hemopoiesis.

[62] 1. 1. Goksan T., Zekeriyaoglu A., Ak I. The Growth of Spirulina platensis in

Different Culture Systems Under Greenhouse Condition. Turkey Journal of Biology. 2007. P. 47-52.

[63] 1. 2. Hamidreza R., Werner S. Great successes in the lowering of pH value in the small intestine of mono gastric animals by using natural feed additives and lowering the macro elements. Tagungsband 4. Boku-Symposium, 2005. P.114-119.

[64] 1. 3. Patil G.; Chethana S.; Madhusudhan M. C; Raghavarao K. S. M. S. Fractionation and purification of the phycobiliproteins from Spirulina platensis. Bioresource Technology, 2008, Vol. 99. P. 7393-7396

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φfflcouHaHHHa. Boπpocbi iBrraHHa, 2006. T. 75. N. 2. C. 19-21.

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Claims

[Claim 1] 1. The fodder additive or supplement comprising algae, characterized in that it comprises cyanobacteria Spirulina platensis in the form of fresh biomass, containing, on the dry matter basis, the blue pigment phy- cocyanin and molasses at the following ratio of components (% by mass): from 13 to 40 percent Spirulina platensis biomass; the rest is molasses; and all this components optionally are mixed with a desired animal fodder.

2. The fodder comprising algae, characterized in that it comprises cy&nob&cteή&Spirulina platensis in the form of fresh biomass, containing, on the dry matter basis, the blue pigment phycocyanin and molasses at the following ratio of components (% by mass): from 0,13 to 40 percent Spirulina platensis biomass; the rest is molasses and animal fodder.

3. The production method of the fodder additive, supplement or fodder according to claim 1 and 2, characterized in that the Spirulina platensis biomass is washed with drinking water, vacuumed to 50-75 % moisture and preserved by mixing it for 30 min with molasses heated up to 36-42 0C, left for at least 24 hours and only then it is mixed into the animal fodder optionally in the amount of 7 % and/or granulated.

The fodder additive, supplement or fodder comprising algae, characterized in that it comprises cyanobacteria Spirulina platensism the form of air dry biomass which contains, on the dry matter basis, the blue pigmentphycocyaninand optionally additionally comprises the ani- malfodderat the following ratio of components (% by mass): from 0,13 to 40 percent Spirulina platensis biomass; the rest is animal fodder. The production method of fodder additive, supplement or fodder according to claim 4, characterized in that the fresh cyanobacteria Spirulina platensishiomass is washed with drinking water, dried to air dry material, then cyanobacteria Spirulina platensis optionally is mixed into the animal fodder in the amount of up to 40 % and/or granulated.