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WO2010007031A3 - Procédés pour améliorer la différenciation cardiaque des cellules souches embryonnaires humaines - Google Patents

Procédés pour améliorer la différenciation cardiaque des cellules souches embryonnaires humaines Download PDF

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Publication number
WO2010007031A3
WO2010007031A3 PCT/EP2009/058936 EP2009058936W WO2010007031A3 WO 2010007031 A3 WO2010007031 A3 WO 2010007031A3 EP 2009058936 W EP2009058936 W EP 2009058936W WO 2010007031 A3 WO2010007031 A3 WO 2010007031A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
stem cells
embryonic stem
human embryonic
improving cardiac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2009/058936
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English (en)
Other versions
WO2010007031A2 (fr
Inventor
Thanh Tran
Mark Burcin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Publication of WO2010007031A2 publication Critical patent/WO2010007031A2/fr
Publication of WO2010007031A3 publication Critical patent/WO2010007031A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cardiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des procédés pour induire une différenciation des cellules souches dans des cardiomyocytes ou autres lignées cellulaires différenciées définies par mise en contact des cellules souches avec Wnt3a ou un fragment fonctionnel de celui-ci. L’invention décrit également des méthodes de traitement de patients atteints de lésions myocardiques, par ex., après un infarctus du myocarde ou une insuffisance cardiaque.
PCT/EP2009/058936 2008-07-14 2009-07-13 Procédés pour améliorer la différenciation cardiaque des cellules souches embryonnaires humaines Ceased WO2010007031A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8057608P 2008-07-14 2008-07-14
US61/080,576 2008-07-14

Publications (2)

Publication Number Publication Date
WO2010007031A2 WO2010007031A2 (fr) 2010-01-21
WO2010007031A3 true WO2010007031A3 (fr) 2010-03-11

Family

ID=41404272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/058936 Ceased WO2010007031A2 (fr) 2008-07-14 2009-07-13 Procédés pour améliorer la différenciation cardiaque des cellules souches embryonnaires humaines

Country Status (1)

Country Link
WO (1) WO2010007031A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011056416A2 (fr) 2009-10-19 2011-05-12 Cellular Dynamics International, Inc. Production de cardiomyocytes
CN104774804B (zh) * 2010-06-13 2018-10-26 中国科学院生物物理研究所 由干细胞制备心肌细胞的方法和组合物及其用途
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
EP2694644B1 (fr) 2011-03-30 2018-01-24 Cellular Dynamics International, Inc. Amorçage de cellules souches pluripotentes pour la différentiation neurale
WO2013010965A1 (fr) * 2011-07-15 2013-01-24 Universite Libre De Bruxelles Génération de cellules mésodermiques à partir de cellules souches pluripotentes
CN107460164B (zh) 2012-07-23 2021-02-05 中国科学院生物物理研究所 体外诱导多能干细胞分化为心室肌细胞的方法
JP6366582B2 (ja) * 2012-10-23 2018-08-01 国立大学法人京都大学 効率的に人工多能性幹細胞を樹立する方法
GB201304831D0 (en) * 2013-03-15 2013-05-01 Coretherapix Slu Adult cardiac stem cell population
JP6682429B2 (ja) * 2013-06-11 2020-04-15 エンカルディア・ベー・フェー 多能性哺乳動物幹細胞に由来する心筋細胞を成熟させるための培地組成物
US10144915B2 (en) 2013-10-23 2018-12-04 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Reprogramming fibroblasts into cardiomyocytes
AU2014277667B2 (en) * 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
MX2018011875A (es) * 2016-03-30 2019-03-28 The J David Gladstone Inst A Testamentary Trust Established Under The Will Of J David Gladstone Reprogramacion cardiaca directa mejorada.
EP3444331A1 (fr) * 2017-08-14 2019-02-20 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Génération facilitée de cardiomyocytes par programmation future de cellules souches pluripotentes humaines
US20220073883A1 (en) * 2018-07-25 2022-03-10 The Johns Hopkins University Compositions and methods for generation of heart field-specific progenitor cells
DE102019127604A1 (de) * 2019-10-14 2021-04-15 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Herstellung von Skelettmuskelzellen und Skelettmuskelgewebe aus pluripotenten Stammzellen
CN115551994A (zh) 2020-03-11 2022-12-30 倍特博有限公司 产生肝细胞的方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040014209A1 (en) * 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
WO2007126077A1 (fr) * 2006-04-28 2007-11-08 Asubio Pharma Co., Ltd. Procédé pour induction de la différenciation de cellules du myocarde à partir de cellules souches pluripotentes
WO2008060446A2 (fr) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Procédés d'induction de la cardiomyogenèse

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040014209A1 (en) * 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
WO2007126077A1 (fr) * 2006-04-28 2007-11-08 Asubio Pharma Co., Ltd. Procédé pour induction de la différenciation de cellules du myocarde à partir de cellules souches pluripotentes
WO2008060446A2 (fr) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Procédés d'induction de la cardiomyogenèse

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ANTON ET AL: "beta-Catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 581, no. 27, 15 October 2007 (2007-10-15), pages 5247 - 5254, XP022324687, ISSN: 0014-5793 *
BAKRE M M ET AL: "Generation of multipotential mesendodermal progenitors from mouse embryonic stem cells via sustained Wnt pathway activation", JOURNAL OF BIOLOGICAL CHEMISTRY 20071026 US, vol. 282, no. 43, 26 October 2007 (2007-10-26), pages 31703 - 31712, XP002561091 *
CAI LIUHONG ET AL: "Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions.", CELL RESEARCH JAN 2007, vol. 17, no. 1, January 2007 (2007-01-01), pages 62 - 72, XP002561094, ISSN: 1748-7838 *
DEB ARJUN ET AL: "SFRP2 regulates cardiomyogenic differentiation by inhibiting a positive transcriptional autofeedback loop of Wnt3a", STEM CELLS (MIAMISBURG), vol. 26, no. 1, 4 October 2007 (2007-10-04), pages 35 - 44, XP002561092, ISSN: 1066-5099 *
DRAVID GAUTAM ET AL: "Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells", STEM CELLS (MIAMISBURG), vol. 23, no. 10, November 2005 (2005-11-01), pages 1489 - 1501, XP002561093, ISSN: 1066-5099 *
KATTMAN STEVEN J ET AL: "Multipotent Flk-1(+) cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages", DEVELOPMENTAL CELL, CELL PRESS, vol. 11, no. 5, 1 November 2006 (2006-11-01), pages 723 - 732, XP002491235, ISSN: 1534-5807 *
TRAN THANH H ET AL: "Wnt3a-induced mesoderm formation and cardiomyogenesis in human embryonic stem cells.", STEM CELLS (DAYTON, OHIO) AUG 2009, vol. 27, no. 8, August 2009 (2009-08-01), pages 1869 - 1878, XP002561096, ISSN: 1549-4918 *
WATANABE KIICHI ET AL: "Directed differentiation of telencephalic precursors from embryonic stem cells", NATURE NEUROSCIENCE, NATURE AMERICA, INC, US, vol. 8, no. 3, 1 March 2005 (2005-03-01), pages 288 - 296, XP002487263, ISSN: 1097-6256 *
YANG LEI ET AL: "Human cardiovascular progenitor cells develop from a KDR plus embryonic-stem-cell-derived population", NATURE (LONDON), vol. 453, no. 7194, May 2008 (2008-05-01), pages 524, XP002561095, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO2010007031A2 (fr) 2010-01-21

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