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WO2010007031A3 - Methods for improving cardiac differentiation of human embryonic stem cells - Google Patents

Methods for improving cardiac differentiation of human embryonic stem cells Download PDF

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Publication number
WO2010007031A3
WO2010007031A3 PCT/EP2009/058936 EP2009058936W WO2010007031A3 WO 2010007031 A3 WO2010007031 A3 WO 2010007031A3 EP 2009058936 W EP2009058936 W EP 2009058936W WO 2010007031 A3 WO2010007031 A3 WO 2010007031A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
stem cells
embryonic stem
human embryonic
improving cardiac
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2009/058936
Other languages
French (fr)
Other versions
WO2010007031A2 (en
Inventor
Thanh Tran
Mark Burcin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Novartis AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis AG filed Critical Novartis AG
Publication of WO2010007031A2 publication Critical patent/WO2010007031A2/en
Publication of WO2010007031A3 publication Critical patent/WO2010007031A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0657—Cardiomyocytes; Heart cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Rheumatology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cardiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Described herein are methods for inducing differentiation of stem cells into cardiomyocytes or other defined differentiated lineages by contacting the stem cells with Wnt3a or a functional fragment thereof. Also described herein are methods for treating patients with myocardial damage, e.g., after myocardial infarction or heart failure.
PCT/EP2009/058936 2008-07-14 2009-07-13 Methods for improving cardiac differentiation of human embryonic stem cells Ceased WO2010007031A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8057608P 2008-07-14 2008-07-14
US61/080,576 2008-07-14

Publications (2)

Publication Number Publication Date
WO2010007031A2 WO2010007031A2 (en) 2010-01-21
WO2010007031A3 true WO2010007031A3 (en) 2010-03-11

Family

ID=41404272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2009/058936 Ceased WO2010007031A2 (en) 2008-07-14 2009-07-13 Methods for improving cardiac differentiation of human embryonic stem cells

Country Status (1)

Country Link
WO (1) WO2010007031A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8415155B2 (en) 2009-10-19 2013-04-09 Cellular Dynamics International, Inc. Cardiomyocyte production
CA2802526C (en) 2010-06-13 2021-11-16 Institute Of Biophysics, Chinese Academy Of Sciences Methods and compositions for preparing cardiomyocytes from stem cells and uses thereof
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
US9487752B2 (en) 2011-03-30 2016-11-08 Cellular Dynamics International, Inc. Priming of pluripotent stem cells for neural differentiation
WO2013010965A1 (en) * 2011-07-15 2013-01-24 Universite Libre De Bruxelles Generation of mesodermal cells from pluripotent stem cells
CN104508121B (en) 2012-07-23 2018-01-19 中国科学院生物物理研究所 Method for inducing pluripotent stem cells to differentiate into ventricular myocytes in vitro
JP6366582B2 (en) * 2012-10-23 2018-08-01 国立大学法人京都大学 A method for efficiently establishing induced pluripotent stem cells
GB201304831D0 (en) 2013-03-15 2013-05-01 Coretherapix Slu Adult cardiac stem cell population
CA2914922C (en) 2013-06-11 2021-11-30 Pluriomics B.V. Culture medium compositions for maturating cardiomyocytes derived from pluripotent mammalian stem cells.
EP3060649A4 (en) 2013-10-23 2017-07-12 The J. David Gladstone Institutes, A Testamentary Trust Established under The Will of J. David Gladstone Reprogramming cardiomyocytes with one transcription factor
AU2014277667B2 (en) * 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
CA3019570A1 (en) * 2016-03-30 2017-10-05 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Enhanced direct cardiac reprogramming
EP3444331A1 (en) * 2017-08-14 2019-02-20 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Facilitated generation of cardiomyocytes by forward programming of human pluripotent stem cells
WO2020023806A1 (en) * 2018-07-25 2020-01-30 The Johns Hopkins University Compositions and methods for generation of heart field-specific progenitor cells
DE102019127604A1 (en) * 2019-10-14 2021-04-15 Georg-August-Universität Göttingen Stiftung Öffentlichen Rechts, Universitätsmedizin Production of skeletal muscle cells and tissue from pluripotent stem cells
CN115551994A (en) 2020-03-11 2022-12-30 倍特博有限公司 Method for producing hepatocytes

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040014209A1 (en) * 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
WO2007126077A1 (en) * 2006-04-28 2007-11-08 Asubio Pharma Co., Ltd. Method for differentiation induction of myocardial cell from pluripotent stem cell
WO2008060446A2 (en) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Methods for inducing cardiomyogenesis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040014209A1 (en) * 2002-01-23 2004-01-22 Lassar Andrew B. Compositions and methods for modulating cell differentiation
WO2007126077A1 (en) * 2006-04-28 2007-11-08 Asubio Pharma Co., Ltd. Method for differentiation induction of myocardial cell from pluripotent stem cell
WO2008060446A2 (en) * 2006-11-09 2008-05-22 The J. David Gladstone Institutes Methods for inducing cardiomyogenesis

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
ANTON ET AL: "beta-Catenin signaling contributes to stemness and regulates early differentiation in murine embryonic stem cells", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 581, no. 27, 15 October 2007 (2007-10-15), pages 5247 - 5254, XP022324687, ISSN: 0014-5793 *
BAKRE M M ET AL: "Generation of multipotential mesendodermal progenitors from mouse embryonic stem cells via sustained Wnt pathway activation", JOURNAL OF BIOLOGICAL CHEMISTRY 20071026 US, vol. 282, no. 43, 26 October 2007 (2007-10-26), pages 31703 - 31712, XP002561091 *
CAI LIUHONG ET AL: "Promoting human embryonic stem cell renewal or differentiation by modulating Wnt signal and culture conditions.", CELL RESEARCH JAN 2007, vol. 17, no. 1, January 2007 (2007-01-01), pages 62 - 72, XP002561094, ISSN: 1748-7838 *
DEB ARJUN ET AL: "SFRP2 regulates cardiomyogenic differentiation by inhibiting a positive transcriptional autofeedback loop of Wnt3a", STEM CELLS (MIAMISBURG), vol. 26, no. 1, 4 October 2007 (2007-10-04), pages 35 - 44, XP002561092, ISSN: 1066-5099 *
DRAVID GAUTAM ET AL: "Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells", STEM CELLS (MIAMISBURG), vol. 23, no. 10, November 2005 (2005-11-01), pages 1489 - 1501, XP002561093, ISSN: 1066-5099 *
KATTMAN STEVEN J ET AL: "Multipotent Flk-1(+) cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages", DEVELOPMENTAL CELL, CELL PRESS, vol. 11, no. 5, 1 November 2006 (2006-11-01), pages 723 - 732, XP002491235, ISSN: 1534-5807 *
TRAN THANH H ET AL: "Wnt3a-induced mesoderm formation and cardiomyogenesis in human embryonic stem cells.", STEM CELLS (DAYTON, OHIO) AUG 2009, vol. 27, no. 8, August 2009 (2009-08-01), pages 1869 - 1878, XP002561096, ISSN: 1549-4918 *
WATANABE KIICHI ET AL: "Directed differentiation of telencephalic precursors from embryonic stem cells", NATURE NEUROSCIENCE, NATURE AMERICA, INC, US, vol. 8, no. 3, 1 March 2005 (2005-03-01), pages 288 - 296, XP002487263, ISSN: 1097-6256 *
YANG LEI ET AL: "Human cardiovascular progenitor cells develop from a KDR plus embryonic-stem-cell-derived population", NATURE (LONDON), vol. 453, no. 7194, May 2008 (2008-05-01), pages 524, XP002561095, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO2010007031A2 (en) 2010-01-21

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