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WO2010049807A3 - Procédé d'expression recombinante de polypeptides - Google Patents

Procédé d'expression recombinante de polypeptides Download PDF

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Publication number
WO2010049807A3
WO2010049807A3 PCT/IB2009/007384 IB2009007384W WO2010049807A3 WO 2010049807 A3 WO2010049807 A3 WO 2010049807A3 IB 2009007384 W IB2009007384 W IB 2009007384W WO 2010049807 A3 WO2010049807 A3 WO 2010049807A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
vector system
recombinant expression
vector
based vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IB2009/007384
Other languages
English (en)
Other versions
WO2010049807A2 (fr
Inventor
Youming Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Bridges GmbH
Original Assignee
Gene Bridges GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Bridges GmbH filed Critical Gene Bridges GmbH
Priority to CN2009801528939A priority Critical patent/CN102264905A/zh
Publication of WO2010049807A2 publication Critical patent/WO2010049807A2/fr
Publication of WO2010049807A3 publication Critical patent/WO2010049807A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un système de deux vecteurs permettant l'expression, pouvant être induite par un large spectre d'hôtes, d'un polypeptide d'intérêt. Ledit système comprend : i) un premier vecteur comprenant : (a) le promoteur T7 positionné en 5' d'un promoteur inductible; (b) un site de clonage positionné en 3' du promoteur inductible pour introduire un gène codant pour le polypeptide d'intérêt; et ii) un second vecteur qui rend une cellule hôte compétente pour exprimer l'ARN polymérase T7, ledit vecteur comprenant un gène codant pour l'ARN polymérase T7 sous la commande d'un promoteur inductible.
PCT/IB2009/007384 2008-10-30 2009-10-29 Procédé d'expression recombinante de polypeptides Ceased WO2010049807A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN2009801528939A CN102264905A (zh) 2008-10-30 2009-10-29 用于多肽重组表达的基于t7双启动子的载体系统

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10985408P 2008-10-30 2008-10-30
US61/109,854 2008-10-30

Publications (2)

Publication Number Publication Date
WO2010049807A2 WO2010049807A2 (fr) 2010-05-06
WO2010049807A3 true WO2010049807A3 (fr) 2010-08-26

Family

ID=41720606

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2009/007384 Ceased WO2010049807A2 (fr) 2008-10-30 2009-10-29 Procédé d'expression recombinante de polypeptides

Country Status (2)

Country Link
CN (1) CN102264905A (fr)
WO (1) WO2010049807A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2628793A1 (fr) * 2012-02-17 2013-08-21 Pharma-Zentrale Gmbh Souches de Escherichia Coli recombinantes
CN103145848B (zh) * 2013-02-06 2014-11-26 上海高龙生物科技有限公司 重组乳铁蛋白二肽、表达载体及其应用
WO2016180379A1 (fr) * 2015-05-14 2016-11-17 Universidad De Los Andes Nouveau transposon favorisant l'expression fonctionnelle de gènes dans des adn épisomaux et procédé d'augmentation de la transcription d'adn dans des analyses fonctionnelles de bibliothèques métagénomiques
CN105779488B (zh) * 2016-03-23 2018-06-22 清华大学 一种诱导外源基因在革兰氏阴性菌中表达的系统及其应用
EP3330282A1 (fr) * 2016-12-02 2018-06-06 Ludwig-Maximilians-Universität München Cipa et cipb pixa comme échafaudages pour organiser des protéines dans des inclusions cristallines
GB201704659D0 (en) * 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
CN107212155B (zh) * 2017-06-16 2019-12-17 西南大学 一种用于鲤科家鱼养殖的药物组合物
CN115197954B (zh) * 2021-04-14 2024-09-17 上海凯赛生物技术股份有限公司 用于发酵生产1,5-戊二胺的重组dna、菌株及其用途
CN115536158B (zh) * 2022-09-21 2024-05-10 华南农业大学 一种复合酶及降解四环素的应用
CN116769814B (zh) * 2023-06-16 2024-02-02 苏州泓迅生物科技股份有限公司 一种大肠杆菌益生菌t7表达系统及其应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571691A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Production of recombinant lactoferrin and lactoferrin polypeptides using CDNA sequences in various organisms

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GARCIA G A ET AL: "EXPRESSION OF PROTEINS IN E. COLI UTILIZING A DUAL PROMOTER-BASED VECTOR: PLACT7", METHODS IN MOLECULAR BIOLOGY, HUMANA PRESS INC, NJ, US, vol. 62, 1 January 1997 (1997-01-01), pages 63 - 71, XP008054745, ISSN: 1064-3745 *
POULTRY SCIENCE, vol. 86, no. Suppl. 1, 2007, JOINT ANNUAL MEETING OF THE AMERICAN-DAIRY-SCIENCE-ASSOCIATION/POULTR Y-SCIENCE-ASSOCIATION-ASOCIACIO; SAN ANTONIO, TX, USA; JULY 08 -12, 2007, pages 440, ISSN: 0032-5791 *
SALAM MOHAMMAD ABDUS ET AL: "Immunogenicity of Salmonella vector vaccines expressing SBR of Streptococcus mutans under the control of a T7-nirB (dual) promoter system", VACCINE, vol. 24, no. 23, June 2006 (2006-06-01), pages 5003 - 5015, XP002572599, ISSN: 0264-410X *
TANG Z R ET AL: "Production of the recombinant bovine lactoferricin and its beneficial supplementation to the diet for weaned pigs", POULTRY SCIENCE, vol. 86, no. Suppl. 1, 2007, & JOINT ANNUAL MEETING OF THE AMERICAN-DAIRY-SCIENCE-ASSOCIATION/POULTR Y-SCIENCE-ASSOCIATION-ASOCIACIO; SAN ANTONIO, TX, USA; JULY 08 -12, 2007, pages 440, XP002590211, ISSN: 0032-5791 *
WELZ D ET AL: "Ferric citrate transport of Escherichia coli: functional regions of the FecR transmembrane regulatory protein.", JOURNAL OF BACTERIOLOGY MAY 1998, vol. 180, no. 9, May 1998 (1998-05-01), pages 2387 - 2394, XP002572618, ISSN: 0021-9193 *

Also Published As

Publication number Publication date
WO2010049807A2 (fr) 2010-05-06
CN102264905A (zh) 2011-11-30

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