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WO2008011234A3 - Hôte recombinant pour obtention de l-asparaginase ii - Google Patents

Hôte recombinant pour obtention de l-asparaginase ii Download PDF

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Publication number
WO2008011234A3
WO2008011234A3 PCT/US2007/070706 US2007070706W WO2008011234A3 WO 2008011234 A3 WO2008011234 A3 WO 2008011234A3 US 2007070706 W US2007070706 W US 2007070706W WO 2008011234 A3 WO2008011234 A3 WO 2008011234A3
Authority
WO
WIPO (PCT)
Prior art keywords
asparaginase
producing
enzyme
recombinant host
escherichia coli
Prior art date
Application number
PCT/US2007/070706
Other languages
English (en)
Other versions
WO2008011234A2 (fr
Inventor
David R Filpula
Maoliang Wang
Original Assignee
Enzon Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to NZ572519A priority Critical patent/NZ572519A/en
Priority to ES07840229.4T priority patent/ES2553329T3/es
Priority to CA002652718A priority patent/CA2652718A1/fr
Priority to CN2007800248607A priority patent/CN101484181B/zh
Priority to DK07840229.4T priority patent/DK2046369T3/en
Priority to PL07840229T priority patent/PL2046369T3/pl
Priority to KR1020087028792A priority patent/KR101408062B1/ko
Priority to AU2007275535A priority patent/AU2007275535B2/en
Application filed by Enzon Pharmaceuticals Inc filed Critical Enzon Pharmaceuticals Inc
Priority to MX2009000284A priority patent/MX2009000284A/es
Priority to EP07840229.4A priority patent/EP2046369B1/fr
Priority to JP2009518433A priority patent/JP5308333B2/ja
Publication of WO2008011234A2 publication Critical patent/WO2008011234A2/fr
Publication of WO2008011234A3 publication Critical patent/WO2008011234A3/fr
Priority to IL195168A priority patent/IL195168A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

L'invention concerne l'utilisation d'une cellule hôte recombinante d'Escherichia coli pour l'obtention d'une enzyme L-asparaginase II d'Escherichia coli. Cette cellule hôte comprend un chromosome d'Eschericia coli et au moins une copie d'un vecteur extrachromosomique recombinant, vecteur qui code pour l'enzyme L-asparaginase II. Le chromosome de cellule hôte code également pour l'enzyme L-asparaginase II, mais ne code pas tout autre isoforme de L-asparaginase II.
PCT/US2007/070706 2006-06-30 2007-06-08 Hôte recombinant pour obtention de l-asparaginase ii WO2008011234A2 (fr)

Priority Applications (12)

Application Number Priority Date Filing Date Title
KR1020087028792A KR101408062B1 (ko) 2006-06-30 2007-06-08 Ｌ-아스파라기나아제 ⅱ 제조를 위한 재조합 숙주
CA002652718A CA2652718A1 (fr) 2006-06-30 2007-06-08 Hote recombinant pour obtention de l-asparaginase ii
CN2007800248607A CN101484181B (zh) 2006-06-30 2007-06-08 用于生产l-天门冬酰胺酶ⅱ的重组宿主
DK07840229.4T DK2046369T3 (en) 2006-06-30 2007-06-08 RECOMBINANT HOST FOR PREPARING L-ASPARAGINASE II
PL07840229T PL2046369T3 (pl) 2006-06-30 2007-06-08 Rekombinowany gospodarz służący do wytwarzania L-Asparaginazy II
NZ572519A NZ572519A (en) 2006-06-30 2007-06-08 Recombinant host for producing l-asparaginase ii
EP07840229.4A EP2046369B1 (fr) 2006-06-30 2007-06-08 Hôte recombinant pour obtention de l-asparaginase ii
AU2007275535A AU2007275535B2 (en) 2006-06-30 2007-06-08 Recombinant host for producing L-asparaginase II
MX2009000284A MX2009000284A (es) 2006-06-30 2007-06-08 Hospedero recombinante para produccion de l-asparaginasa ii.
ES07840229.4T ES2553329T3 (es) 2006-06-30 2007-06-08 Huésped recombinante para la producción de L-asparaginasa II
JP2009518433A JP5308333B2 (ja) 2006-06-30 2007-06-08 Ｌ−アスパラギナーゼｉｉを産生する組換え宿主
IL195168A IL195168A (en) 2006-06-30 2008-11-09 Recombinant sonic ashtray cell for l-asparagine production ii

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81781706P 2006-06-30 2006-06-30
US60/817,817 2006-06-30

Publications (2)

Publication Number Publication Date
WO2008011234A2 WO2008011234A2 (fr) 2008-01-24
WO2008011234A3 true WO2008011234A3 (fr) 2008-10-16

Family

ID=38957454

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/070706 WO2008011234A2 (fr) 2006-06-30 2007-06-08 Hôte recombinant pour obtention de l-asparaginase ii

Country Status (17)

Country Link
US (2) US7807436B2 (fr)
EP (1) EP2046369B1 (fr)
JP (1) JP5308333B2 (fr)
KR (1) KR101408062B1 (fr)
CN (1) CN101484181B (fr)
AU (1) AU2007275535B2 (fr)
CA (1) CA2652718A1 (fr)
DK (1) DK2046369T3 (fr)
ES (1) ES2553329T3 (fr)
HU (1) HUE025738T2 (fr)
IL (1) IL195168A (fr)
MX (1) MX2009000284A (fr)
NZ (1) NZ572519A (fr)
PL (1) PL2046369T3 (fr)
PT (1) PT2046369E (fr)
TW (1) TWI427148B (fr)
WO (1) WO2008011234A2 (fr)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR102014000585B1 (pt) 2014-01-10 2021-01-12 Coppe/Ufrj-Instituto Alberto Luiz Coimbra De Pós-Graduação l-asparaginase recombinante de zymomonas
CN105802948B (zh) 2014-12-29 2020-06-09 江苏众红生物工程创药研究院有限公司 聚乙二醇定点修饰的门冬酰胺酶及其制备方法与应用
WO2016204503A1 (fr) * 2015-06-15 2016-12-22 아주대학교 산학협력단 Procédé pour la production de caoutchouc naturel au moyen de micro-organisme recombinant
MX388814B (es) 2016-06-01 2025-03-20 Servier Ip Uk Ltd Formulaciones de oxido de polialquileno-asparaginasa y metodos de preparacion y uso del mismo.
KR101837636B1 (ko) * 2016-06-14 2018-03-12 전남대학교산학협력단 코리네박테리움 재조합 균주를 이용한 아스파라기나제의 분비생산 방법
CN108795832B (zh) * 2017-04-26 2021-05-14 江苏恒瑞医药股份有限公司 一种内源l-门冬酰胺酶ii基因敲除的宿主菌、其制备方法及其应用
CN111278852B (zh) 2017-10-27 2024-07-16 菲尼克斯公司 重组欧氏杆菌天冬酰胺酶的生产方法
SG11202003614WA (en) 2017-10-27 2020-05-28 Pfenex Inc Method for production of recombinant e. coli asparaginase
EP3893870A1 (fr) 2018-12-13 2021-10-20 Huya Bioscience International LLC Administration de sulcardine pour le traitement d'une fibrillation atriale aiguë
EP4267592A1 (fr) 2020-12-23 2023-11-01 Jazz Pharmaceuticals Ireland Ltd. Procédés de purification de protéines de fusion protégées par une charge
CN114854729B (zh) * 2021-02-03 2025-09-02 重庆派金生物科技有限公司 定向化学偶联门冬酰胺酶突变体及其制备方法和应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5122614A (en) * 1989-04-19 1992-06-16 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5324844A (en) * 1989-04-19 1994-06-28 Enzon, Inc. Active carbonates of polyalkylene oxides for modification of polypeptides
US5643575A (en) * 1993-10-27 1997-07-01 Enzon, Inc. Non-antigenic branched polymer conjugates
US5919455A (en) * 1993-10-27 1999-07-06 Enzon, Inc. Non-antigenic branched polymer conjugates
US5615460A (en) * 1994-06-06 1997-04-01 The Procter & Gamble Company Female component for refastenable fastening device having regions of differential extensibility
US6180095B1 (en) * 1997-12-17 2001-01-30 Enzon, Inc. Polymeric prodrugs of amino- and hydroxyl-containing bioactive agents
US5965119A (en) * 1997-12-30 1999-10-12 Enzon, Inc. Trialkyl-lock-facilitated polymeric prodrugs of amino-containing bioactive agents
US6958212B1 (en) * 1999-02-01 2005-10-25 Eidgenossische Technische Hochschule Zurich Conjugate addition reactions for the controlled delivery of pharmaceutically active compounds
US7087229B2 (en) * 2003-05-30 2006-08-08 Enzon Pharmaceuticals, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers
US7122189B2 (en) * 2002-08-13 2006-10-17 Enzon, Inc. Releasable polymeric conjugates based on aliphatic biodegradable linkers
CN100502944C (zh) * 2005-09-16 2009-06-24 北京双鹭药业股份有限公司 一种天门冬酰胺酶注射液、其制备方法及应用

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
WANG ET AL.: "Cloning and Expression of L-Asparaginase Gene in Escherichia coli", APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, vol. 95, no. 2, 2001, pages 93 - 101, XP008101539 *

Also Published As

Publication number Publication date
JP2009542219A (ja) 2009-12-03
KR101408062B1 (ko) 2014-06-20
WO2008011234A2 (fr) 2008-01-24
US20080063632A1 (en) 2008-03-13
CN101484181A (zh) 2009-07-15
US20100273236A1 (en) 2010-10-28
TWI427148B (zh) 2014-02-21
ES2553329T3 (es) 2015-12-07
IL195168A (en) 2014-12-31
EP2046369A4 (fr) 2010-04-21
DK2046369T3 (en) 2015-11-30
NZ572519A (en) 2011-06-30
KR20090033175A (ko) 2009-04-01
CA2652718A1 (fr) 2008-01-24
CN101484181B (zh) 2012-12-26
AU2007275535A1 (en) 2008-01-24
HUE025738T2 (en) 2016-04-28
PT2046369E (pt) 2015-11-26
TW200817518A (en) 2008-04-16
PL2046369T3 (pl) 2016-01-29
MX2009000284A (es) 2009-01-26
EP2046369A2 (fr) 2009-04-15
IL195168A0 (en) 2011-08-01
EP2046369B1 (fr) 2015-08-12
US7807436B2 (en) 2010-10-05
AU2007275535B2 (en) 2012-08-16
JP5308333B2 (ja) 2013-10-09

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