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WO2009016842A1 - 単一細胞捕捉用マイクロ流路デバイス - Google Patents

単一細胞捕捉用マイクロ流路デバイス Download PDF

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Publication number
WO2009016842A1
WO2009016842A1 PCT/JP2008/002058 JP2008002058W WO2009016842A1 WO 2009016842 A1 WO2009016842 A1 WO 2009016842A1 JP 2008002058 W JP2008002058 W JP 2008002058W WO 2009016842 A1 WO2009016842 A1 WO 2009016842A1
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Prior art keywords
cells
plastic
microfluidicdevice
micromesh
plate
Prior art date
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Ceased
Application number
PCT/JP2008/002058
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English (en)
French (fr)
Inventor
Tadashi Matsunaga
Atsushi Arakaki
Tomoyuki Taguchi
Masahito Hosokawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tokyo University of Agriculture and Technology NUC
University of Tokyo NUC
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Tokyo University of Agriculture and Technology NUC
University of Tokyo NUC
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Application filed by Tokyo University of Agriculture and Technology NUC, University of Tokyo NUC filed Critical Tokyo University of Agriculture and Technology NUC
Priority to EP08790322.5A priority Critical patent/EP2175014B1/en
Priority to US12/670,990 priority patent/US8357530B2/en
Priority to JP2009525288A priority patent/JP5278913B2/ja
Publication of WO2009016842A1 publication Critical patent/WO2009016842A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Signal Processing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

微細加工技術を利用して、試料中の大量の細胞を、ダメージを与えることなく高効率で１細胞レベルで分離・捕捉するマイクロ流路デバイス（microfluidicdevice）を提供するものである。試料供給口と試料排出口を連通するマイクロ流路形成用の溝が設けられたプラスチック製平板からなる上部基板と、前記上部基板と協働してマイクロ流路を形成するとともに、マイクロメッシュ用開口窓が設けられたプラスチック製上部平板と、細胞捕捉用の複数の微細貫通孔を有するプラスチック製のマイクロメッシュを保持した保持平板と、吸引用開口窓が設けられたプラスチック製下部平板と、吸引流路形成用の溝が設けられたプラスチック製平板からなる下部基板とを順次備え、前記マイクロメッシュの細胞捕捉用の微細貫通孔の形状が反すり鉢状又は円筒状であり、前記上部平板を前記上部基板に比較して軟質のプラスチックで構成する。
PCT/JP2008/002058 2007-08-01 2008-07-31 単一細胞捕捉用マイクロ流路デバイス Ceased WO2009016842A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08790322.5A EP2175014B1 (en) 2007-08-01 2008-07-31 Microfluidic device for trapping single cell
US12/670,990 US8357530B2 (en) 2007-08-01 2008-07-31 Microfluidic device for trapping single cell
JP2009525288A JP5278913B2 (ja) 2007-08-01 2008-07-31 単一細胞捕捉用マイクロ流路デバイス

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007201373 2007-08-01
JP2007-201373 2007-08-01

Publications (1)

Publication Number Publication Date
WO2009016842A1 true WO2009016842A1 (ja) 2009-02-05

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PCT/JP2008/002058 Ceased WO2009016842A1 (ja) 2007-08-01 2008-07-31 単一細胞捕捉用マイクロ流路デバイス

Country Status (4)

Country Link
US (1) US8357530B2 (ja)
EP (1) EP2175014B1 (ja)
JP (1) JP5278913B2 (ja)
WO (1) WO2009016842A1 (ja)

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JP2011163830A (ja) * 2010-02-05 2011-08-25 Tokyo Univ Of Agriculture & Technology サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
JP2012000065A (ja) * 2010-06-18 2012-01-05 Konica Minolta Holdings Inc 細胞配列システム及び細胞配列方法
JP2012088055A (ja) * 2010-09-21 2012-05-10 Tokyo Univ Of Agriculture & Technology 微量血液からの白血球ポピュレーション解析法
JP2012177686A (ja) * 2011-01-31 2012-09-13 Nokodai Tlo Kk 細胞解析装置及び細胞解析方法
WO2012141157A1 (ja) * 2011-04-11 2012-10-18 株式会社日立製作所 細胞採取システム
JP2013042689A (ja) * 2011-08-23 2013-03-04 Hitachi Chemical Co Ltd 癌細胞濃縮フィルター
WO2014141386A1 (ja) * 2013-03-12 2014-09-18 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
JP2014223058A (ja) * 2013-04-22 2014-12-04 日立化成株式会社 がん細胞捕捉装置、処理液キット及び処理液キットの製造方法
JP2015097499A (ja) * 2013-11-19 2015-05-28 日立化成株式会社 細胞捕捉システム及び細胞捕捉システムの運転方法
WO2015084257A1 (en) * 2013-12-04 2015-06-11 Clearbridge Mfluidics Pte. Ltd. A microfluidic device
WO2015093116A1 (ja) * 2013-12-17 2015-06-25 コニカミノルタ株式会社 細胞の蛍光免疫染色方法ならびにそのためのシステムおよびキット
JPWO2014061631A1 (ja) * 2012-10-15 2016-09-05 国立大学法人名古屋大学 微粒子分離用マイクロ流路チップ、該チップを用いた微粒子分離用システム及び微粒子分離方法
JP2017035117A (ja) * 2016-11-22 2017-02-16 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
WO2018105608A1 (ja) * 2016-12-07 2018-06-14 東京応化工業株式会社 粒子捕捉デバイス
WO2018159008A1 (ja) * 2017-03-02 2018-09-07 株式会社日立製作所 洗浄機能付き単一細胞解析装置
WO2020035981A1 (ja) * 2018-08-15 2020-02-20 ソニー株式会社 粒子捕捉用チャンバ及び粒子捕捉方法
WO2020158178A1 (ja) * 2019-01-28 2020-08-06 ソニー株式会社 粒子捕捉装置、粒子捕捉方法、及び顕微鏡システム
US11299727B2 (en) 2016-09-05 2022-04-12 Hitachi, Ltd. System for analyzing single cell
WO2022210056A1 (ja) * 2021-03-30 2022-10-06 東レ株式会社 フィルター及びその製造方法、フィルターデバイス、稀少細胞を分離または分取する方法、並びに細胞懸濁液中の稀少細胞の分析方法
WO2023210798A1 (ja) * 2022-04-28 2023-11-02 公立大学法人大阪 微生物の集積方法および集積システム

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Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011163830A (ja) * 2010-02-05 2011-08-25 Tokyo Univ Of Agriculture & Technology サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
JP2012000065A (ja) * 2010-06-18 2012-01-05 Konica Minolta Holdings Inc 細胞配列システム及び細胞配列方法
JP2012088055A (ja) * 2010-09-21 2012-05-10 Tokyo Univ Of Agriculture & Technology 微量血液からの白血球ポピュレーション解析法
JP2012177686A (ja) * 2011-01-31 2012-09-13 Nokodai Tlo Kk 細胞解析装置及び細胞解析方法
WO2012141157A1 (ja) * 2011-04-11 2012-10-18 株式会社日立製作所 細胞採取システム
JP2012217397A (ja) * 2011-04-11 2012-11-12 Hitachi Ltd 細胞採取システム
JP2013042689A (ja) * 2011-08-23 2013-03-04 Hitachi Chemical Co Ltd 癌細胞濃縮フィルター
JPWO2014061631A1 (ja) * 2012-10-15 2016-09-05 国立大学法人名古屋大学 微粒子分離用マイクロ流路チップ、該チップを用いた微粒子分離用システム及び微粒子分離方法
JPWO2014141386A1 (ja) * 2013-03-12 2017-02-16 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
US10030240B2 (en) 2013-03-12 2018-07-24 Hitachi, Ltd. Two-dimensional cell array device and apparatus for gene quantification and sequence analysis
WO2014141386A1 (ja) * 2013-03-12 2014-09-18 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
JP2014223058A (ja) * 2013-04-22 2014-12-04 日立化成株式会社 がん細胞捕捉装置、処理液キット及び処理液キットの製造方法
JP2015097499A (ja) * 2013-11-19 2015-05-28 日立化成株式会社 細胞捕捉システム及び細胞捕捉システムの運転方法
CN105814189A (zh) * 2013-12-04 2016-07-27 明策微型流体私人有限公司 微流体装置
WO2015084257A1 (en) * 2013-12-04 2015-06-11 Clearbridge Mfluidics Pte. Ltd. A microfluidic device
US9849456B2 (en) 2013-12-04 2017-12-26 Clearbridge Mfluidics Pte. Ltd. Microfluidic device
WO2015093116A1 (ja) * 2013-12-17 2015-06-25 コニカミノルタ株式会社 細胞の蛍光免疫染色方法ならびにそのためのシステムおよびキット
JPWO2015093116A1 (ja) * 2013-12-17 2017-03-16 コニカミノルタ株式会社 細胞の蛍光免疫染色方法ならびにそのためのシステムおよびキット
US11299727B2 (en) 2016-09-05 2022-04-12 Hitachi, Ltd. System for analyzing single cell
JP2017035117A (ja) * 2016-11-22 2017-02-16 株式会社日立製作所 遺伝子定量・配列解析用２次元セルアレイデバイスおよび装置
JPWO2018105608A1 (ja) * 2016-12-07 2019-10-24 東京応化工業株式会社 粒子捕捉デバイス
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