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WO2009011139A1 - 細胞の単離方法、細胞用無血清培養培地および細胞の培養方法 - Google Patents

細胞の単離方法、細胞用無血清培養培地および細胞の培養方法 Download PDF

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Publication number
WO2009011139A1
WO2009011139A1 PCT/JP2008/050278 JP2008050278W WO2009011139A1 WO 2009011139 A1 WO2009011139 A1 WO 2009011139A1 JP 2008050278 W JP2008050278 W JP 2008050278W WO 2009011139 A1 WO2009011139 A1 WO 2009011139A1
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Prior art keywords
cell
serum
isolation
culture
culture medium
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Ceased
Application number
PCT/JP2008/050278
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English (en)
French (fr)
Inventor
Seiichi Yokoo
Satoru Yamagami
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Mitsubishi Tanabe Pharma Corp
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Mitsubishi Tanabe Pharma Corp
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Priority to JP2009523552A priority Critical patent/JP5409359B2/ja
Priority to EP08703142.3A priority patent/EP2177602B1/en
Priority to US12/668,744 priority patent/US20100184221A1/en
Publication of WO2009011139A1 publication Critical patent/WO2009011139A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/38—Vitamins
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/395—Thyroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Ophthalmology & Optometry (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

動物由来の組織を細胞に解離させる工程、該細胞を表面処理されていない培養容器に播種してインキュベートする工程、および容器に付着した細胞を選択する工程を含む細胞、特に幹細胞の単離方法。
PCT/JP2008/050278 2007-07-13 2008-01-11 細胞の単離方法、細胞用無血清培養培地および細胞の培養方法 Ceased WO2009011139A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
JP2009523552A JP5409359B2 (ja) 2007-07-13 2008-01-11 細胞の単離方法、細胞用無血清培養培地および細胞の培養方法
EP08703142.3A EP2177602B1 (en) 2007-07-13 2008-01-11 Method for isolation of cell, serum-free culture medium for cell, and method for culture of cell
US12/668,744 US20100184221A1 (en) 2007-07-13 2008-01-11 Method for isolation of cell, serum-free culture medium for cell, and method for culture of cell

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-184897 2007-07-13
JP2007184897 2007-07-13

Publications (1)

Publication Number Publication Date
WO2009011139A1 true WO2009011139A1 (ja) 2009-01-22

Family

ID=40259485

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/050278 Ceased WO2009011139A1 (ja) 2007-07-13 2008-01-11 細胞の単離方法、細胞用無血清培養培地および細胞の培養方法

Country Status (4)

Country Link
US (1) US20100184221A1 (ja)
EP (1) EP2177602B1 (ja)
JP (1) JP5409359B2 (ja)
WO (1) WO2009011139A1 (ja)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009017847A (ja) * 2007-07-13 2009-01-29 Nipro Corp 細胞培養培地の製造方法及び細胞培養培地
US20120141410A1 (en) * 2009-03-11 2012-06-07 Invitrx, Inc. Method and composition for the treatment of moderate to severe keratoconjunctivitis sicca
JP2012518409A (ja) * 2009-02-20 2012-08-16 ベントリア・バイオサイエンス タンパク質の組み合わせを含有する細胞培養培地
JP2013517776A (ja) * 2010-01-25 2013-05-20 ベントリア・バイオサイエンス タンパク質の生成を改善するための方法および組成物
WO2013136372A1 (ja) * 2012-03-16 2013-09-19 株式会社日立製作所 細胞シート、細胞培養方法および細胞培養装置
WO2017022809A1 (ja) * 2015-08-03 2017-02-09 国立大学法人大阪大学 間葉系幹細胞由来エキソソーム
JPWO2016159179A1 (ja) * 2015-03-30 2018-01-25 味の素株式会社 ヒト血清アルブミンを含む神経幹細胞増殖培地
WO2019225672A1 (ja) * 2018-05-23 2019-11-28 国立大学法人大阪大学 多能性幹細胞由来結膜細胞の誘導方法
US11479754B2 (en) 2016-04-20 2022-10-25 Kyoto Prefectural Public University Corporation Method for producing cultivated epithelial cell sheet

Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
CN103492555A (zh) * 2011-04-20 2014-01-01 国立大学法人大阪大学 角膜上皮分化取向性iPS细胞
LT2788472T (lt) * 2011-12-06 2019-04-10 Astellas Institute For Regenerative Medicine Kryptingos diferenciacijos būdas, skirtas ragenos endotelio ląstelių gamybai
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
KR102211266B1 (ko) 2013-01-31 2021-02-04 아지노모토 가부시키가이샤 다능성 줄기 세포의 안정한 미분화 유지 증식을 실시하기 위한 배양 방법
JP6206792B2 (ja) * 2013-04-03 2017-10-04 誠一 横尾 培地及び細胞の培養方法
EP3437628A4 (en) * 2016-04-01 2019-11-27 Biocoz Global Korea Corp. COSMETIC COMPOSITION USING ALBUMINE, HYALURONIC ACID OR COLLAGEN AS AN ACTIVE IN CELL CULTURE MEDIUM
WO2020018868A1 (en) 2018-07-19 2020-01-23 Texas Tech University System Corneal epithelial cells and their products for treating corneal diseases
CN112029712A (zh) * 2019-05-14 2020-12-04 北京泰生生物科技有限公司 一种牙龈干细胞的分离、培养方法及其应用
CN114755413A (zh) * 2022-04-19 2022-07-15 中国计量大学 基于纳米酶探针的dmp和dbp快速可视化检测方法

Citations (1)

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WO2005059113A1 (en) * 2003-12-19 2005-06-30 Omnicyte Ltd Stem cells

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CA2248142A1 (en) * 1996-03-12 1997-09-18 Life Technologies, Inc. Hematopoietic cell culture nutrient supplement
US5945337A (en) * 1996-10-18 1999-08-31 Quality Biological, Inc. Method for culturing CD34+ cells in a serum-free medium
EP1812556A4 (en) * 2004-10-05 2011-02-02 Univ Georgia NEURONAL PRECURSORS OF FEEDER-FREE HUMAN EMBRYONIC STEM CELL CULTURE
WO2007130060A2 (en) * 2006-05-03 2007-11-15 Schepens Eye Research Isolation and therapeutic application of adult retinal stem cells collected from extra-retinal tissues

Patent Citations (1)

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WO2005059113A1 (en) * 2003-12-19 2005-06-30 Omnicyte Ltd Stem cells

Non-Patent Citations (4)

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"Adult stem cells isolated from human corneas for clinical application", CELL TECHNOLOGY, vol. 26, no. 5, 22 April 2007 (2007-04-22), pages 532 - 537 *
ROUGER, K. ET AL.: "Progenitor cell isolation from muscle-derived cells based onadhesion properties", THE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, vol. 55, no. 6, June 2007 (2007-06-01), pages 607 - 618, XP008128385 *
See also references of EP2177602A4 *
YIQIN, LI LINGSONG: "Reconstruction of chemically burned rat corneal surface by bone marrow- derived human mesenchymal stem cells", STEM CELLS, vol. 24, no. 2, February 2006 (2006-02-01), pages 315 - 321, XP008128384 *

Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009017847A (ja) * 2007-07-13 2009-01-29 Nipro Corp 細胞培養培地の製造方法及び細胞培養培地
JP2012518409A (ja) * 2009-02-20 2012-08-16 ベントリア・バイオサイエンス タンパク質の組み合わせを含有する細胞培養培地
US20120141410A1 (en) * 2009-03-11 2012-06-07 Invitrx, Inc. Method and composition for the treatment of moderate to severe keratoconjunctivitis sicca
JP2013517776A (ja) * 2010-01-25 2013-05-20 ベントリア・バイオサイエンス タンパク質の生成を改善するための方法および組成物
WO2013136372A1 (ja) * 2012-03-16 2013-09-19 株式会社日立製作所 細胞シート、細胞培養方法および細胞培養装置
JPWO2013136372A1 (ja) * 2012-03-16 2015-07-30 株式会社日立製作所 細胞シート、細胞培養方法および細胞培養装置
EP3279318A4 (en) * 2015-03-30 2018-08-29 Ajinomoto Co., Inc. Human serum albumin-containing culture medium for growth of neural stem cells
JPWO2016159179A1 (ja) * 2015-03-30 2018-01-25 味の素株式会社 ヒト血清アルブミンを含む神経幹細胞増殖培地
JPWO2017022809A1 (ja) * 2015-08-03 2018-05-24 国立大学法人大阪大学 間葉系幹細胞由来エキソソーム
WO2017022809A1 (ja) * 2015-08-03 2017-02-09 国立大学法人大阪大学 間葉系幹細胞由来エキソソーム
US11357798B2 (en) 2015-08-03 2022-06-14 Osaka University Mesenchymal stem cell-derived exosome
US11479754B2 (en) 2016-04-20 2022-10-25 Kyoto Prefectural Public University Corporation Method for producing cultivated epithelial cell sheet
WO2019225672A1 (ja) * 2018-05-23 2019-11-28 国立大学法人大阪大学 多能性幹細胞由来結膜細胞の誘導方法
CN112189049A (zh) * 2018-05-23 2021-01-05 国立大学法人大阪大学 来源于多能干细胞的结膜细胞的诱导方法
JPWO2019225672A1 (ja) * 2018-05-23 2021-05-13 国立大学法人大阪大学 多能性幹細胞由来結膜細胞の誘導方法
JP7107595B2 (ja) 2018-05-23 2022-07-27 国立大学法人大阪大学 多能性幹細胞由来結膜細胞の誘導方法
CN112189049B (zh) * 2018-05-23 2023-12-15 国立大学法人大阪大学 来源于多能干细胞的结膜细胞的诱导方法

Also Published As

Publication number Publication date
US20100184221A1 (en) 2010-07-22
EP2177602A4 (en) 2010-08-11
EP2177602B1 (en) 2013-09-11
JPWO2009011139A1 (ja) 2010-09-16
JP5409359B2 (ja) 2014-02-05
EP2177602A1 (en) 2010-04-21

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