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WO2009004664A3 - Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof - Google Patents

Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof Download PDF

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Publication number
WO2009004664A3
WO2009004664A3 PCT/IT2008/000439 IT2008000439W WO2009004664A3 WO 2009004664 A3 WO2009004664 A3 WO 2009004664A3 IT 2008000439 W IT2008000439 W IT 2008000439W WO 2009004664 A3 WO2009004664 A3 WO 2009004664A3
Authority
WO
WIPO (PCT)
Prior art keywords
preparation
stem cells
cell line
immortalized cell
mesenchymal stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2008/000439
Other languages
French (fr)
Other versions
WO2009004664A2 (en
WO2009004664A8 (en
Inventor
Nardo Paolo Di
Giancarlo Forte
Ornella Franzese
Enzo Bonmassar
Maria Prat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universita degli Studi di Roma Tor Vergata
Universita degli Studi di Roma La Sapienza
Original Assignee
Universita degli Studi di Roma Tor Vergata
Universita degli Studi di Roma La Sapienza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universita degli Studi di Roma Tor Vergata, Universita degli Studi di Roma La Sapienza filed Critical Universita degli Studi di Roma Tor Vergata
Publication of WO2009004664A2 publication Critical patent/WO2009004664A2/en
Publication of WO2009004664A3 publication Critical patent/WO2009004664A3/en
Publication of WO2009004664A8 publication Critical patent/WO2009004664A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention concerns a new immortalized cell line of mesenchymal murine stem cells, method for preparation and uses thereof, particularly as an experimental model.
PCT/IT2008/000439 2007-07-03 2008-06-27 Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof Ceased WO2009004664A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM20070372 ITRM20070372A1 (en) 2007-07-03 2007-07-03 IMMORTALIZED CELL LINE OF MESENCHIMAL MURINE STEM CELLS, METHOD FOR ITS PREPARATION AND RELATED USES.
ITRM2007A000372 2007-07-03

Publications (3)

Publication Number Publication Date
WO2009004664A2 WO2009004664A2 (en) 2009-01-08
WO2009004664A3 true WO2009004664A3 (en) 2009-02-19
WO2009004664A8 WO2009004664A8 (en) 2009-07-23

Family

ID=39941464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2008/000439 Ceased WO2009004664A2 (en) 2007-07-03 2008-06-27 Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof

Country Status (2)

Country Link
IT (1) ITRM20070372A1 (en)
WO (1) WO2009004664A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107937442A (en) * 2017-11-13 2018-04-20 北京多赢时代科技有限公司 A kind of immortal human fat mesenchymal stem cell system and its method for building up
CN110373382B (en) * 2019-06-12 2022-07-15 秦海燕 Method for extracting ips cell extract and method for reversing senescent mesenchymal stem cells by using ips cell extract

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1447443A1 (en) * 2001-10-31 2004-08-18 Renomedix Institute Inc. Immortalized mesenchymal cells and utilization thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1447443A1 (en) * 2001-10-31 2004-08-18 Renomedix Institute Inc. Immortalized mesenchymal cells and utilization thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BAKSH D ET AL: "Adult mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy", JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, vol. 8, no. 3, July 2004 (2004-07-01), pages 301 - 316, XP002504675, ISSN: 1582-1838 *
FORTE G ET AL: "Generation of a novel Sca-1(pos) Lin(neg) murine mesenchymal stem cell immortalized line for tissue engineering applications", TISSUE ENGINEERING, LARCHMONT, NY, US, vol. 14, no. 5, 1 May 2008 (2008-05-01), pages 856 - 857, XP009108773, ISSN: 1076-3279 *
LEE MING KEI ET AL: "Ectopic mTERT expression in mouse embryonic stem cells does not affect differentiation but confers resistance to differentiation- and stress-induced p53-dependent apoptosis", JOURNAL OF CELL SCIENCE, vol. 118, no. 4, February 2005 (2005-02-01), pages 819 - 829, XP002504676, ISSN: 0021-9533 *
SHI SONGTAO ET AL: "Bone formation by human postnatal bone marrow stromal stem cells is enhanced by telomerase expression", NATURE BIOTECHNOLOGY, vol. 20, no. 6, June 2002 (2002-06-01), pages 587 - 591, XP002504674, ISSN: 1087-0156 *
SIMONSEN J L ET AL: "Telomerase expression extends the proliferactive life-span and maintains the osteogenic potential of human bone marrow stromal cells", NATURE BIOTECHNOLOGY, NATURE PUBLISHING GROUP, NEW YORK, NY, US, vol. 20, 1 June 2002 (2002-06-01), pages 592 - 596, XP002902749, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2009004664A2 (en) 2009-01-08
ITRM20070372A1 (en) 2009-01-04
WO2009004664A8 (en) 2009-07-23

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