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WO2009004664A8 - Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof - Google Patents

Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof Download PDF

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Publication number
WO2009004664A8
WO2009004664A8 PCT/IT2008/000439 IT2008000439W WO2009004664A8 WO 2009004664 A8 WO2009004664 A8 WO 2009004664A8 IT 2008000439 W IT2008000439 W IT 2008000439W WO 2009004664 A8 WO2009004664 A8 WO 2009004664A8
Authority
WO
WIPO (PCT)
Prior art keywords
preparation
stem cells
cell line
immortalized cell
mesenchymal stem
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IT2008/000439
Other languages
French (fr)
Other versions
WO2009004664A2 (en
WO2009004664A3 (en
Inventor
Nardo Paolo Di
Giancarlo Forte
Ornella Franzese
Enzo Bonmassar
Maria Prat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universita degli Studi di Roma Tor Vergata
Universita degli Studi di Roma La Sapienza
Original Assignee
Universita degli Studi di Roma Tor Vergata
Universita degli Studi di Roma La Sapienza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universita degli Studi di Roma Tor Vergata, Universita degli Studi di Roma La Sapienza filed Critical Universita degli Studi di Roma Tor Vergata
Publication of WO2009004664A2 publication Critical patent/WO2009004664A2/en
Publication of WO2009004664A3 publication Critical patent/WO2009004664A3/en
Publication of WO2009004664A8 publication Critical patent/WO2009004664A8/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Rheumatology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention concerns a new immortalized cell line of mesenchymal murine stem cells, method for preparation and uses thereof, particularly as an experimental model.
PCT/IT2008/000439 2007-07-03 2008-06-27 Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof Ceased WO2009004664A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ITRM20070372 ITRM20070372A1 (en) 2007-07-03 2007-07-03 IMMORTALIZED CELL LINE OF MESENCHIMAL MURINE STEM CELLS, METHOD FOR ITS PREPARATION AND RELATED USES.
ITRM2007A000372 2007-07-03

Publications (3)

Publication Number Publication Date
WO2009004664A2 WO2009004664A2 (en) 2009-01-08
WO2009004664A3 WO2009004664A3 (en) 2009-02-19
WO2009004664A8 true WO2009004664A8 (en) 2009-07-23

Family

ID=39941464

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IT2008/000439 Ceased WO2009004664A2 (en) 2007-07-03 2008-06-27 Immortalized cell line of murine mesenchymal stem cells, method for preparation and uses thereof

Country Status (2)

Country Link
IT (1) ITRM20070372A1 (en)
WO (1) WO2009004664A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107937442A (en) * 2017-11-13 2018-04-20 北京多赢时代科技有限公司 A kind of immortal human fat mesenchymal stem cell system and its method for building up

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110373382B (en) * 2019-06-12 2022-07-15 秦海燕 Method for extracting ips cell extract and method for reversing senescent mesenchymal stem cells by using ips cell extract

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2003038076A1 (en) * 2001-10-31 2005-02-24 株式会社レノメディクス研究所 Immortalized mesenchymal cells and their use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107937442A (en) * 2017-11-13 2018-04-20 北京多赢时代科技有限公司 A kind of immortal human fat mesenchymal stem cell system and its method for building up

Also Published As

Publication number Publication date
ITRM20070372A1 (en) 2009-01-04
WO2009004664A2 (en) 2009-01-08
WO2009004664A3 (en) 2009-02-19

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