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WO2009054320A1 - 核酸プローブ及びプローブポリマーの形成方法 - Google Patents

核酸プローブ及びプローブポリマーの形成方法 Download PDF

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Publication number
WO2009054320A1
WO2009054320A1 PCT/JP2008/068849 JP2008068849W WO2009054320A1 WO 2009054320 A1 WO2009054320 A1 WO 2009054320A1 JP 2008068849 W JP2008068849 W JP 2008068849W WO 2009054320 A1 WO2009054320 A1 WO 2009054320A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
probe
polymer
acid probe
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/068849
Other languages
English (en)
French (fr)
Inventor
Mitsugu Usui
Chikako Hakii
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eisai R&D Management Co Ltd
Original Assignee
Eisai R&D Management Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R&D Management Co Ltd filed Critical Eisai R&D Management Co Ltd
Priority to EP08842746A priority Critical patent/EP2202319A4/en
Priority to US12/734,235 priority patent/US20100256007A1/en
Priority to CN200880113346A priority patent/CN101835906A/zh
Priority to JP2009538167A priority patent/JPWO2009054320A1/ja
Publication of WO2009054320A1 publication Critical patent/WO2009054320A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　核酸プローブのポリマーを簡便且つ効率的に形成することができるプローブポリマーの形成方法、該方法で形成されるプローブポリマー、該方法で用いられる新規の核酸プローブ、及び高感度且つ簡便に標的分析物を検出することができる標的分析物の検出方法を提供する。３以上の核酸領域を含む核酸プローブであって、該核酸プローブの各核酸領域が第１領域及び該第１領域に相補的な第２領域を隣接して含むようにした。該核酸プローブを反応させることにより、該核酸プローブのポリマーが形成される。
PCT/JP2008/068849 2007-10-24 2008-10-17 核酸プローブ及びプローブポリマーの形成方法 Ceased WO2009054320A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP08842746A EP2202319A4 (en) 2007-10-24 2008-10-17 NUCLEIC ACID PROBE AND PROCESS FOR PRODUCING POLYMER FOR PROBE
US12/734,235 US20100256007A1 (en) 2007-10-24 2008-10-17 Nucleic acid probe, and method of forming probe-polymer
CN200880113346A CN101835906A (zh) 2007-10-24 2008-10-17 核酸探针及探针聚合物的形成方法
JP2009538167A JPWO2009054320A1 (ja) 2007-10-24 2008-10-17 核酸プローブ及びプローブポリマーの形成方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007276681 2007-10-24
JP2007-276681 2007-10-24

Publications (1)

Publication Number Publication Date
WO2009054320A1 true WO2009054320A1 (ja) 2009-04-30

Family

ID=40579426

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068849 Ceased WO2009054320A1 (ja) 2007-10-24 2008-10-17 核酸プローブ及びプローブポリマーの形成方法

Country Status (7)

Country Link
US (1) US20100256007A1 (ja)
EP (1) EP2202319A4 (ja)
JP (1) JPWO2009054320A1 (ja)
KR (1) KR20100085923A (ja)
CN (1) CN101835906A (ja)
SG (1) SG185315A1 (ja)
WO (1) WO2009054320A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013172305A1 (ja) 2012-05-15 2013-11-21 エーザイ・アール・アンド・ディー・マネジメント株式会社 Ｒｎａの検出方法及び検出用キット

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2789688A4 (en) * 2011-12-09 2015-07-15 Eisai R&D Man Co Ltd METHOD FOR DETECTING NUCLEOTIDE MUTATION AND DETECTION KIT
JP6995250B2 (ja) * 2020-01-31 2022-02-04 積水メディカル株式会社 核酸の検出又は定量方法

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450594A2 (en) * 1990-04-03 1991-10-09 David Segev DNA probe signal amplification
WO1992020702A1 (en) 1991-05-24 1992-11-26 Ole Buchardt Peptide nucleic acids
WO2001075157A1 (en) * 2000-03-31 2001-10-11 Sanko Junyaku Co., Ltd. Probe for constructing probe polymer, method of constructing probe polymer and utilization thereof
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
WO2002031192A1 (en) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Method of constructing self-assembly of probes and method of detecting the same
JP2002355081A (ja) 2001-03-21 2002-12-10 Sanko Junyaku Kk オリゴヌクレオチドによる自己集合体の作製方法及び遺伝子の検出方法
JP2003061679A (ja) * 2001-08-29 2003-03-04 Sanko Junyaku Kk オリゴヌクレオチドの巨視的な自己集合体の検出方法及びその形成方法。
WO2003029441A1 (en) 2001-09-28 2003-04-10 Sanko Junyaku Co., Ltd. Method of amplifying dna chip signals
JP2003116598A (ja) * 2001-10-09 2003-04-22 Sanko Junyaku Kk 遺伝子の検出方法
WO2005106031A1 (ja) 2004-04-28 2005-11-10 Eisai R & D Management Co., Ltd. ハイブリダイゼーション方法
WO2007037282A1 (ja) * 2005-09-27 2007-04-05 Eisai R & D Management Co., Ltd. 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法
WO2007108378A1 (ja) * 2006-03-15 2007-09-27 Eisai R & D Management Co., Ltd. シグナルプローブポリマーの形成方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020065471A (ko) * 2000-08-30 2002-08-13 산코 준야쿠 가부시키가이샤 유전자의 검출방법
JPWO2003040367A1 (ja) * 2001-11-08 2005-03-03 三光純薬株式会社 遺伝子増幅反応で合成されたオリゴヌクレオチドによる自己集合体の形成方法、自己集合体及び遺伝子の検出方法
AU2006219362B2 (en) * 2005-02-28 2011-08-25 Sekisui Medical Co., Ltd. Assist probes and method of using the same

Patent Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0450594A2 (en) * 1990-04-03 1991-10-09 David Segev DNA probe signal amplification
WO1992020702A1 (en) 1991-05-24 1992-11-26 Ole Buchardt Peptide nucleic acids
JP3267576B2 (ja) 1998-11-09 2002-03-18 三光純薬株式会社 プローブ高分子の形成方法
WO2001075157A1 (en) * 2000-03-31 2001-10-11 Sanko Junyaku Co., Ltd. Probe for constructing probe polymer, method of constructing probe polymer and utilization thereof
JP3310662B2 (ja) 2000-03-31 2002-08-05 三光純薬株式会社 プローブポリマー作製用プローブ、プローブポリマーの作製方法及びその利用
WO2002031192A1 (en) 2000-10-11 2002-04-18 Sanko Junyaku Co., Ltd. Method of constructing self-assembly of probes and method of detecting the same
JP2002355081A (ja) 2001-03-21 2002-12-10 Sanko Junyaku Kk オリゴヌクレオチドによる自己集合体の作製方法及び遺伝子の検出方法
JP2003061679A (ja) * 2001-08-29 2003-03-04 Sanko Junyaku Kk オリゴヌクレオチドの巨視的な自己集合体の検出方法及びその形成方法。
WO2003029441A1 (en) 2001-09-28 2003-04-10 Sanko Junyaku Co., Ltd. Method of amplifying dna chip signals
JP2003116598A (ja) * 2001-10-09 2003-04-22 Sanko Junyaku Kk 遺伝子の検出方法
WO2005106031A1 (ja) 2004-04-28 2005-11-10 Eisai R & D Management Co., Ltd. ハイブリダイゼーション方法
WO2007037282A1 (ja) * 2005-09-27 2007-04-05 Eisai R & D Management Co., Ltd. 微小粒子に自己集合体を形成させる方法及び標的分析物の検出方法
WO2007108378A1 (ja) * 2006-03-15 2007-09-27 Eisai R & D Management Co., Ltd. シグナルプローブポリマーの形成方法

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
KOSHKIN AA ET AL., J. AM. CHEM. SOC., vol. 120, 1998, pages 13252 - 13253
KOSHKIN AA ET AL., TETRAHEDRON, vol. 54, 1998, pages 3607 - 3630
MARSHALL, A.; HODGSON, J.: "DNA chips: an array of possibilities", NAT BIOTECHNOL., vol. 16, 1998, pages 27 - 31
See also references of EP2202319A4
WAHLESTEDT C ET AL., PNAS, vol. 97, 2000, pages 5633 - 5638

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013172305A1 (ja) 2012-05-15 2013-11-21 エーザイ・アール・アンド・ディー・マネジメント株式会社 Ｒｎａの検出方法及び検出用キット

Also Published As

Publication number Publication date
JPWO2009054320A1 (ja) 2011-03-03
EP2202319A4 (en) 2010-10-20
CN101835906A (zh) 2010-09-15
EP2202319A1 (en) 2010-06-30
SG185315A1 (en) 2012-11-29
KR20100085923A (ko) 2010-07-29
US20100256007A1 (en) 2010-10-07

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