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WO2009048067A1 - 糖鎖を持たない遺伝子組換え血液凝固第x因子およびその製法 - Google Patents

糖鎖を持たない遺伝子組換え血液凝固第x因子およびその製法 Download PDF

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Publication number
WO2009048067A1
WO2009048067A1 PCT/JP2008/068271 JP2008068271W WO2009048067A1 WO 2009048067 A1 WO2009048067 A1 WO 2009048067A1 JP 2008068271 W JP2008068271 W JP 2008068271W WO 2009048067 A1 WO2009048067 A1 WO 2009048067A1
Authority
WO
WIPO (PCT)
Prior art keywords
sugar chain
genetically engineered
blood coagulation
coagulation factor
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/068271
Other languages
English (en)
French (fr)
Inventor
Kenji Soejima
Takayuki Imamura
Ryoichi Kawamura
Hiroshi Nakatake
Arisa Maeyashiki
Hitomi Togo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chemo Sero Therapeutic Research Institute Kaketsuken
Original Assignee
Chemo Sero Therapeutic Research Institute Kaketsuken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeutic Research Institute Kaketsuken filed Critical Chemo Sero Therapeutic Research Institute Kaketsuken
Priority to DK08837781.7T priority Critical patent/DK2202306T3/da
Priority to EP08837781A priority patent/EP2202306B1/en
Priority to US12/682,445 priority patent/US8173777B2/en
Priority to JP2009537004A priority patent/JP5513120B2/ja
Publication of WO2009048067A1 publication Critical patent/WO2009048067A1/ja
Anticipated expiration legal-status Critical
Priority to US13/235,076 priority patent/US8293874B2/en
Priority to US13/404,747 priority patent/US8679783B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　高活性化血液凝固第X因子（以下、FXという）を提供する。二本鎖の遺伝子組換えFXの効率的な生産方法であって、FXの糖鎖付加に必須なアミノ酸配列への糖鎖付加を阻害することにより、糖鎖を持たない遺伝子組換えFXを発現させることを特徴とする方法、および該方法により得られる糖鎖を持たない遺伝子組換えFXに関する。
PCT/JP2008/068271 2007-10-09 2008-10-08 糖鎖を持たない遺伝子組換え血液凝固第x因子およびその製法 Ceased WO2009048067A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DK08837781.7T DK2202306T3 (da) 2007-10-09 2008-10-08 Rekombinant faktor x uden glycosylering og fremgangsmåde til fremstilling deraf
EP08837781A EP2202306B1 (en) 2007-10-09 2008-10-08 Recombinant factor x with no glycosylation and method for preparing the same
US12/682,445 US8173777B2 (en) 2007-10-09 2008-10-08 Recombinant Factor X with no glycosylation and method for preparing the same
JP2009537004A JP5513120B2 (ja) 2007-10-09 2008-10-08 糖鎖を持たない遺伝子組換え血液凝固第ｘ因子およびその製法
US13/235,076 US8293874B2 (en) 2007-10-09 2011-09-16 Recombinant factor X with no glycosylation and method for preparing the same
US13/404,747 US8679783B2 (en) 2007-10-09 2012-02-24 Recombinant factor X with no glycosylation and method for preparing the same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007263640 2007-10-09
JP2007-263640 2007-10-09

Related Child Applications (3)

Application Number Title Priority Date Filing Date
US12/682,445 A-371-Of-International US8173777B2 (en) 2007-10-09 2008-10-08 Recombinant Factor X with no glycosylation and method for preparing the same
US13/235,076 Division US8293874B2 (en) 2007-10-09 2011-09-16 Recombinant factor X with no glycosylation and method for preparing the same
US13/404,747 Division US8679783B2 (en) 2007-10-09 2012-02-24 Recombinant factor X with no glycosylation and method for preparing the same

Publications (1)

Publication Number Publication Date
WO2009048067A1 true WO2009048067A1 (ja) 2009-04-16

Family

ID=40549211

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/068271 Ceased WO2009048067A1 (ja) 2007-10-09 2008-10-08 糖鎖を持たない遺伝子組換え血液凝固第x因子およびその製法

Country Status (5)

Country Link
US (3) US8173777B2 (ja)
EP (2) EP2202306B1 (ja)
JP (1) JP5513120B2 (ja)
DK (2) DK2436765T3 (ja)
WO (1) WO2009048067A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9856467B2 (en) 2012-07-25 2018-01-02 Catalyst Biosciences, Inc. Modified factor X polypeptides and uses thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2436765T3 (da) 2007-10-09 2014-01-13 Chemo Sero Therapeut Res Inst Rekombinant faktor X uden glycosylering og fremgangsmåde til fremstilling deraf
KR20120045218A (ko) * 2010-10-29 2012-05-09 삼성전자주식회사 멀티 터치를 이용한 문자 입력 방법 및 장치
WO2014008172A2 (en) * 2012-07-03 2014-01-09 Expression Therapeutics, Llc High yield suspension cell line, system and method for making same

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2824434B2 (ja) 1989-11-28 1998-11-11 財団法人化学及血清療法研究所 新規発現ベクター
JP2005515749A (ja) * 2000-12-05 2005-06-02 ザ ペン ステイト リサーチ ファウンデイション 酵母中での異種たんぱく質の高効率生産のための方法および組成物
WO2006107084A1 (ja) * 2005-03-31 2006-10-12 Yamaguchi University 分泌型耐熱性酵素を高分泌生産できる酵母変異株

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040072757A1 (en) * 1990-09-04 2004-04-15 Cor Therapeutics, Inc. Agents affecting thrombosis and hemostasis
US5278144A (en) * 1990-09-04 1994-01-11 Cor Therapeutics, Inc. Antithrombosis agents
WO1993018782A1 (en) * 1992-03-20 1993-09-30 Cor Therapeutics, Inc. Glycosylation-mediated inhibition of factor x
EP1728798A1 (en) * 2005-06-01 2006-12-06 ZLB Behring GmbH Coagulation factor X polypeptides with modified activation properties
DK2436765T3 (da) * 2007-10-09 2014-01-13 Chemo Sero Therapeut Res Inst Rekombinant faktor X uden glycosylering og fremgangsmåde til fremstilling deraf

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2824434B2 (ja) 1989-11-28 1998-11-11 財団法人化学及血清療法研究所 新規発現ベクター
JP2005515749A (ja) * 2000-12-05 2005-06-02 ザ ペン ステイト リサーチ ファウンデイション 酵母中での異種たんぱく質の高効率生産のための方法および組成物
WO2006107084A1 (ja) * 2005-03-31 2006-10-12 Yamaguchi University 分泌型耐熱性酵素を高分泌生産できる酵母変異株

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BRUCE ALBERTS ET AL: "Molecular Biology of The Cell 2nd edition", GARLAND PUBLISHING, INC.
EDITED BY K. A. HIGH AND H. R. ROBERTS: "Molecular Basis of Thrombosia and Hemostasis", 1995, MARCEL DEKKER, INC
HIMMELSPACH M. ET AL.: "Recombinant human factor X: high yield expression and the role of furin in proteolytic maturation in vivo and in vitro", THROMB RES., vol. 97, 2000, pages 51 - 67, XP000914912 *
JOHN E. COLIGAN ET AL.: "Current Protocols in Protein Science", vol. 2, JOHN WILEY & SONS,INC
KAWASHIMA H. ET AL.: "The effects of N-glycan of human coagulation factor X for the secretion, 4P-0192", SEIKAGAKU, 11 December 2007 (2007-12-11) *
See also references of EP2202306A4

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9856467B2 (en) 2012-07-25 2018-01-02 Catalyst Biosciences, Inc. Modified factor X polypeptides and uses thereof

Also Published As

Publication number Publication date
US8293874B2 (en) 2012-10-23
US20100285568A1 (en) 2010-11-11
US8679783B2 (en) 2014-03-25
DK2436765T3 (da) 2014-01-13
JP5513120B2 (ja) 2014-06-04
EP2202306A4 (en) 2011-03-30
DK2202306T3 (da) 2013-01-21
JPWO2009048067A1 (ja) 2011-02-17
EP2202306B1 (en) 2012-12-19
EP2436765B1 (en) 2013-12-11
EP2202306A1 (en) 2010-06-30
US8173777B2 (en) 2012-05-08
EP2436765A2 (en) 2012-04-04
US20120231523A1 (en) 2012-09-13
EP2436765A3 (en) 2012-05-09
US20120039863A1 (en) 2012-02-16

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