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WO2008105508A1 - ３，６－ジクロロ－ｏ－アニシン酸を含む共存培地で植物組織を培養する共存工程を含む、植物の形質転換効率を上昇させる方法 - Google Patents

３，６－ジクロロ－ｏ－アニシン酸を含む共存培地で植物組織を培養する共存工程を含む、植物の形質転換効率を上昇させる方法 Download PDF

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Publication number
WO2008105508A1
WO2008105508A1 PCT/JP2008/053558 JP2008053558W WO2008105508A1 WO 2008105508 A1 WO2008105508 A1 WO 2008105508A1 JP 2008053558 W JP2008053558 W JP 2008053558W WO 2008105508 A1 WO2008105508 A1 WO 2008105508A1
Authority
WO
WIPO (PCT)
Prior art keywords
plant
dichloro
transformation
efficiency
medium containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2008/053558
Other languages
English (en)
French (fr)
Inventor
Yuji Ishida
Yukoh Hiei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Tobacco Inc
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Priority to AU2008219991A priority Critical patent/AU2008219991B2/en
Priority to CN200880006564.9A priority patent/CN101668418B/zh
Priority to EP08721007.6A priority patent/EP2135503B1/en
Priority to JP2009501305A priority patent/JP5227303B2/ja
Priority to US12/528,965 priority patent/US8101820B2/en
Priority to CA2681662A priority patent/CA2681662C/en
Publication of WO2008105508A1 publication Critical patent/WO2008105508A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明は、従来公知のアグロバクテリウム法に比べて、植物の形質転換効率を上昇させる方法を提供することを課題とする。 　本発明は、アグロバクテリウム菌を接種した植物組織を、３，６－ジクロロ－ｏ－アニシン酸を含む共存培地で培養する共存工程を含むことを特徴の１つとする。
PCT/JP2008/053558 2007-02-28 2008-02-28 ３，６－ジクロロ－ｏ－アニシン酸を含む共存培地で植物組織を培養する共存工程を含む、植物の形質転換効率を上昇させる方法 Ceased WO2008105508A1 (ja)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2008219991A AU2008219991B2 (en) 2007-02-28 2008-02-28 Method for increasing transformation efficiency in plants, comprising coculture step for culturing plant tissue with coculture medium containing 3,6-dichloro-o-anisic acid
CN200880006564.9A CN101668418B (zh) 2007-02-28 2008-02-28 包括用含3,6-二氯-2-甲氧基苯甲酸的共存培养基培养植物组织的共存步骤的提高植物转化效率的方法
EP08721007.6A EP2135503B1 (en) 2007-02-28 2008-02-28 METHOD FOR IMPROVEMENT OF EFFICIENCY OF TRANSFORMATION IN PLANT, COMPRISING CO-CULTURE STEP FOR CULTURING PLANT TISSUE IN CO-CULTURE MEDIUM CONTAINING 3,6-DICHLORO-o-ANISIC ACID
JP2009501305A JP5227303B2 (ja) 2007-02-28 2008-02-28 ３，６−ジクロロ−ｏ−アニシン酸を含む共存培地で植物組織を培養する共存工程を含む、植物の形質転換効率を上昇させる方法
US12/528,965 US8101820B2 (en) 2007-02-28 2008-02-28 Method for increasing transformation efficiency in plants, comprising coculture step for culturing plant tissue with coculture medium containing 3,6-dichloro-o-anisic acid
CA2681662A CA2681662C (en) 2007-02-28 2008-02-28 Method for increasing transformation efficiency in plants, comprising coculture step for culturing plant tissue with coculture medium containing 3,6-dichloro-o-anisic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007049161 2007-02-28
JP2007-049161 2007-02-28

Publications (1)

Publication Number Publication Date
WO2008105508A1 true WO2008105508A1 (ja) 2008-09-04

Family

ID=39721330

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/053558 Ceased WO2008105508A1 (ja) 2007-02-28 2008-02-28 ３，６－ジクロロ－ｏ－アニシン酸を含む共存培地で植物組織を培養する共存工程を含む、植物の形質転換効率を上昇させる方法

Country Status (7)

Country Link
US (1) US8101820B2 (ja)
EP (1) EP2135503B1 (ja)
JP (1) JP5227303B2 (ja)
CN (1) CN101668418B (ja)
AU (1) AU2008219991B2 (ja)
CA (1) CA2681662C (ja)
WO (1) WO2008105508A1 (ja)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102469769A (zh) * 2009-07-29 2012-05-23 日本烟草产业株式会社 使用土壤杆菌对小麦属的植物进行基因导入的方法及小麦属的植物的转化植物的制作方法
CN108441512A (zh) * 2018-04-20 2018-08-24 刘寒冬 一种玉米遗传转化方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UA117654C2 (uk) * 2009-11-06 2018-09-10 Агрівіда, Інк. Трансгенна рослина, яка експресує фермент, що деградує клітинну стінку, і вектор експресії
US20130071884A1 (en) 2009-11-06 2013-03-21 Agrivida, Inc. Plants expressing cell wall degrading enzymes and expression vectors
JP2013212051A (ja) * 2010-07-29 2013-10-17 Japan Tobacco Inc アグロバクテリウム菌を用いた、オオムギ属植物へ遺伝子導入を行う方法およびオオムギ属植物の形質転換植物の作成方法

Citations (7)

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JP2000023675A (ja) 1998-07-14 2000-01-25 Japan Tobacco Inc 広範な品種に適応可能なアグロバクテリウムによるトウモロコシの形質転換方法
JP2000342253A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342256A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
JP2000342255A (ja) 1999-06-04 2000-12-12 Japan Tobacco Inc 植物細胞への遺伝子導入の効率を向上させる方法
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CA2386126C (en) 2000-08-03 2010-07-13 Japan Tobacco Inc. Method of improving gene transfer efficiency into plant cells
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JP2000023675A (ja) 1998-07-14 2000-01-25 Japan Tobacco Inc 広範な品種に適応可能なアグロバクテリウムによるトウモロコシの形質転換方法
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102469769A (zh) * 2009-07-29 2012-05-23 日本烟草产业株式会社 使用土壤杆菌对小麦属的植物进行基因导入的方法及小麦属的植物的转化植物的制作方法
US8884101B2 (en) 2009-07-29 2014-11-11 Japan Tobacco Inc. Method of gene introduction into Triticum plant using Agrobacterium, and a method of producing transformed Triticum plant
CN108441512A (zh) * 2018-04-20 2018-08-24 刘寒冬 一种玉米遗传转化方法

Also Published As

Publication number Publication date
EP2135503B1 (en) 2014-01-22
JPWO2008105508A1 (ja) 2010-06-03
CA2681662C (en) 2015-11-03
US8101820B2 (en) 2012-01-24
EP2135503A1 (en) 2009-12-23
CA2681662A1 (en) 2008-09-04
AU2008219991A1 (en) 2008-09-04
AU2008219991B2 (en) 2014-04-03
JP5227303B2 (ja) 2013-07-03
CN101668418A (zh) 2010-03-10
CN101668418B (zh) 2013-03-20
EP2135503A4 (en) 2010-07-28
US20100068812A1 (en) 2010-03-18

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