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WO2008153676A3 - Séquences nucléotiques codant pour les enzymes de fermentation et de la voie du pentose phosphate présentant une cinétique translationnelle plus fine et procédés de réalisation correspondants - Google Patents

Séquences nucléotiques codant pour les enzymes de fermentation et de la voie du pentose phosphate présentant une cinétique translationnelle plus fine et procédés de réalisation correspondants Download PDF

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Publication number
WO2008153676A3
WO2008153676A3 PCT/US2008/006378 US2008006378W WO2008153676A3 WO 2008153676 A3 WO2008153676 A3 WO 2008153676A3 US 2008006378 W US2008006378 W US 2008006378W WO 2008153676 A3 WO2008153676 A3 WO 2008153676A3
Authority
WO
WIPO (PCT)
Prior art keywords
pentose phosphate
phosphate pathway
expression
fermentation enzyme
encoding nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2008/006378
Other languages
English (en)
Other versions
WO2008153676A2 (fr
Inventor
Kirsty A Salmon
David A Roth
G Wesley Hatfield
Yimeng Dou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2008153676A2 publication Critical patent/WO2008153676A2/fr
Publication of WO2008153676A3 publication Critical patent/WO2008153676A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Cette invention concerne des séquences polynucléotidiques et des gènes de synthèse codant pour les enzymes de fermentation et de la voie du pentose phosphate devant être exprimées dans un organisme hôte avec une cinétique translationnelle plus fine et/ou améliorée, ainsi que des procédés permettant de les réaliser. Le nucléotide codant pour les enzymes de fermentation et/ou de la voie du pentose phosphate est prévu pour être translaté rapidement sur toute sa longueur. L'expression de ce nucléotide ainsi obtenu est prévue pour permettre d'obtenir des niveaux d'expression protéique améliorés dans les cas où les pauses de translation excessives ou inappropriées réduisent l'expression protéique. De plus, l'expression du nucléotide codant pour les enzymes de fermentation et/ou de la voie du pentose phosphate est prévue pour permettre d'obtenir des niveaux améliorés d'expression des polypeptides fonctionnels et repliés d'origine et/ou actifs dans les cas où des pauses de translation excessives ou inappropriées provoquent l'expression d'une enzyme de fermentation et/ou de voie du pentose phosphate inactive, insoluble, agrégée ou quelque peu dysfonctionnelle ou peu active.
PCT/US2008/006378 2007-05-21 2008-05-14 Séquences nucléotiques codant pour les enzymes de fermentation et de la voie du pentose phosphate présentant une cinétique translationnelle plus fine et procédés de réalisation correspondants Ceased WO2008153676A2 (fr)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US93919707P 2007-05-21 2007-05-21
US93920007P 2007-05-21 2007-05-21
US60/939,197 2007-05-21
US60/939,200 2007-05-21
US94064207P 2007-05-29 2007-05-29
US60/940,642 2007-05-29
US94078507P 2007-05-30 2007-05-30
US60/940,785 2007-05-30

Publications (2)

Publication Number Publication Date
WO2008153676A2 WO2008153676A2 (fr) 2008-12-18
WO2008153676A3 true WO2008153676A3 (fr) 2009-03-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2008/006378 Ceased WO2008153676A2 (fr) 2007-05-21 2008-05-14 Séquences nucléotiques codant pour les enzymes de fermentation et de la voie du pentose phosphate présentant une cinétique translationnelle plus fine et procédés de réalisation correspondants

Country Status (1)

Country Link
WO (1) WO2008153676A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116103325A (zh) * 2021-11-10 2023-05-12 北京理工大学 一种提升氨基酸胁迫条件下菌株性能的代谢工程方法

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0723017A2 (fr) * 1995-01-23 1996-07-24 Basf Aktiengesellschaft Transcétolase
WO2000032789A1 (fr) * 1998-12-02 2000-06-08 Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro-Dlo) Genes lies a la saveur des fruits et utilisation de ces genes
WO2002020796A2 (fr) * 2000-09-01 2002-03-14 E.I. Dupont De Nemours And Company Genes et enzymes du mecanisme metabolique methanotrophique du carbone
WO2004042043A2 (fr) * 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Structures cristallines de 3-epimerases bacteriennes de ribulose-phosphate
WO2005003362A2 (fr) * 2003-03-10 2005-01-13 Athenix Corporation Methodes destinees a conferer une resistance aux herbicides
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
WO2006083410A2 (fr) * 2004-12-22 2006-08-10 Michigan Biotechnology Institute Micro-organismes de recombinaison pour une production accrue d'acides organiques
WO2008041840A1 (fr) * 2006-10-02 2008-04-10 Dsm Ip Assets B.V. Génie métabolique de cellules de levure induisant la fermentation de l'arabinose

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0723017A2 (fr) * 1995-01-23 1996-07-24 Basf Aktiengesellschaft Transcétolase
WO2000032789A1 (fr) * 1998-12-02 2000-06-08 Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro-Dlo) Genes lies a la saveur des fruits et utilisation de ces genes
WO2002020796A2 (fr) * 2000-09-01 2002-03-14 E.I. Dupont De Nemours And Company Genes et enzymes du mecanisme metabolique methanotrophique du carbone
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
WO2004042043A2 (fr) * 2002-11-05 2004-05-21 Affinium Pharmaceuticals, Inc. Structures cristallines de 3-epimerases bacteriennes de ribulose-phosphate
WO2005003362A2 (fr) * 2003-03-10 2005-01-13 Athenix Corporation Methodes destinees a conferer une resistance aux herbicides
WO2006083410A2 (fr) * 2004-12-22 2006-08-10 Michigan Biotechnology Institute Micro-organismes de recombinaison pour une production accrue d'acides organiques
WO2008041840A1 (fr) * 2006-10-02 2008-04-10 Dsm Ip Assets B.V. Génie métabolique de cellules de levure induisant la fermentation de l'arabinose

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BECKER JESSICA ET AL: "A MODIFIED SACCHAROMYCES CEREVISIAE STRAIN THAT CONSUMES L-ARABINOSE AND PRODUCES ETHANOL", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, vol. 69, no. 7, 1 July 2003 (2003-07-01), pages 4144 - 4150, XP009080581, ISSN: 0099-2240 *
HAHN-HÄGERDAL BÄRBEL ET AL: "Metabolic engineering for pentose utilization in Saccharomyces cerevisiae.", ADVANCES IN BIOCHEMICAL ENGINEERING/BIOTECHNOLOGY 2007, vol. 108, 12 May 2007 (2007-05-12), pages 147 - 177, XP008095962, ISSN: 0724-6145 *
KARHUMAA KAISA ET AL: "Investigation of limiting metabolic steps in the utilization of xylose by recombinant Saccharomyces cerevisiae using metabolic engineering.", YEAST (CHICHESTER, ENGLAND) 15 APR 2005, vol. 22, no. 5, 15 April 2005 (2005-04-15), pages 359 - 368, XP002494168, ISSN: 0749-503X *
SUNDSTROEM M ET AL: "YEAST TKL1 GENE ENCODES A TRANSKETOLASE THAT IS REQUIRED FOR EFFICIENT GLYCOLYSIS AND BIOSYNTHESIS OF AROMATIC AMINO ACIDS", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM,; US, vol. 268, no. 32, 1 January 1993 (1993-01-01), pages 24346 - 24352, XP000919119, ISSN: 0021-9258 *
VAN MARIS ANTONIUS J A ET AL: "Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: current status.", ANTONIE VAN LEEUWENHOEK NOV 2006, vol. 90, no. 4, November 2006 (2006-11-01), pages 391 - 418, XP002494169, ISSN: 0003-6072 *
WIEDEMANN BEATE ET AL: "Codon-optimized bacterial genes improve L-Arabinose fermentation in recombinant Saccharomyces cerevisiae.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY APR 2008, vol. 74, no. 7, April 2008 (2008-04-01), pages 2043 - 2050, XP002494170, ISSN: 1098-5336 *

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WO2008153676A2 (fr) 2008-12-18

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