[go: up one dir, main page]

WO2008152640A3 - Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches - Google Patents

Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches Download PDF

Info

Publication number
WO2008152640A3
WO2008152640A3 PCT/IL2008/000803 IL2008000803W WO2008152640A3 WO 2008152640 A3 WO2008152640 A3 WO 2008152640A3 IL 2008000803 W IL2008000803 W IL 2008000803W WO 2008152640 A3 WO2008152640 A3 WO 2008152640A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
hematopoietic stem
dimensional
cell culture
stromal cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/IL2008/000803
Other languages
English (en)
Other versions
WO2008152640A2 (fr
Inventor
Shai Meretzki
Uriel Barkai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pluri Biotech Ltd
Original Assignee
Pluristem Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pluristem Ltd filed Critical Pluristem Ltd
Publication of WO2008152640A2 publication Critical patent/WO2008152640A2/fr
Anticipated expiration legal-status Critical
Publication of WO2008152640A3 publication Critical patent/WO2008152640A3/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C12N2533/40—Polyhydroxyacids, e.g. polymers of glycolic or lactic acid (PGA, PLA, PLGA); Bioresorbable polymers

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention porte sur un procédé de transplantation de cellules souches hématopoïétiques non différenciées sur un receveur. Le procédé consiste à (a) faire se développer les cellules souches hématopoïétiques non différenciées pour augmenter le nombre des cellules souches hématopoïétiques par (i) la culture dans un bioréacteur à écoulement piston en phase stationnaire de cellules stromales sous un courant continu d'un milieu de culture établi sur un substrat biocompatible tridimensionnel afin de générer une culture tridimensionnelle de cellules stromales ; et (ii) l'ensemencement de cellules souches hématopoïétiques non différenciées dans le bioréacteur à écoulement piston en phase stationnaire comprenant la culture tridimensionnelle de cellules stromales et sous un courant continu d'un milieu de culture pour développer les cellules souches hématopoïétiques non différenciées et obtenir une culture tridimensionnelle de cellules stromales comprenant un nombre accru de cellules souches hématopoïétique ; et (b) transplanter sur le receveur la culture tridimensionnelle de cellules stromales comprenant des cellules souches hématopoïétiques résultant de (a).
PCT/IL2008/000803 2007-06-13 2008-06-12 Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches Ceased WO2008152640A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US92909707P 2007-06-13 2007-06-13
US60/929,097 2007-06-13

Publications (2)

Publication Number Publication Date
WO2008152640A2 WO2008152640A2 (fr) 2008-12-18
WO2008152640A3 true WO2008152640A3 (fr) 2010-02-25

Family

ID=40130285

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2008/000803 Ceased WO2008152640A2 (fr) 2007-06-13 2008-06-12 Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches

Country Status (1)

Country Link
WO (1) WO2008152640A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9198938B2 (en) 2008-11-19 2015-12-01 Antrhogenesis Corporation Amnion derived adherent cells

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2662676C1 (ru) 2008-08-20 2018-07-26 Антродженезис Корпорейшн Улучшенная клеточная композиция и способы ее получения
GB0822246D0 (en) * 2008-12-05 2009-01-14 Reneuron Ltd Composition
GB0902034D0 (en) 2009-02-06 2009-03-11 Reneuron Ltd Method
US8969315B2 (en) 2010-12-31 2015-03-03 Anthrogenesis Corporation Enhancement of placental stem cell potency using modulatory RNA molecules
DK2714059T3 (en) 2011-06-01 2019-01-21 Celularity Inc TREATMENT OF PAIN WHEN USING PLACENT STAM CELLS
WO2013035101A1 (fr) 2011-09-11 2013-03-14 Minovia Therapeutics Ltd. Compositions de mitochondries fonctionnelles et leurs utilisations
WO2013171752A1 (fr) 2012-05-16 2013-11-21 Minovia Therapeutics Ltd. Compositions et procédés d'induction de l'angiogenèse
CA2977341A1 (fr) 2015-02-26 2016-09-01 Minovia Therapeutics Ltd. Cellules mammiferes enrichies avec des mitochondries fonctionnelles
US12440515B2 (en) 2018-07-22 2025-10-14 Minovia Therapeutics Ltd. Mitochondrial augmentation therapy of brain diseases
WO2020021539A1 (fr) 2018-07-22 2020-01-30 Minovia Therapeutics Ltd. Thérapie d'augmentation mitochondriale de maladies musculaires
JP7586811B2 (ja) 2018-07-22 2024-11-19 ミノヴィア セラピューティクス リミテッド 腎臓疾患のミトコンドリア増強療法
EP3823641A4 (fr) 2018-07-22 2022-05-18 Minovia Therapeutics Ltd. Thérapie d'augmentation mitochondriale de maladies oculaires
EP4095626A1 (fr) 2021-05-27 2022-11-30 Bühler AG Bioréacteur industriel optimisé et procédé associé, avec des boucles de commande de processus couplées mutuellement dépendantes
BR112023022517A2 (pt) 2021-05-27 2024-01-02 Buehler Ag Biorreator industrial otimizado e método do mesmo, com circuitos de controle de processo acoplados, mutuamente dependentes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6498138B1 (en) * 1998-03-11 2002-12-24 University Of Southern California Method of promoting production of living tissue equivalents
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo
US20050180958A1 (en) * 1999-02-04 2005-08-18 Technion Research & Development Foundation Ltd. Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6498138B1 (en) * 1998-03-11 2002-12-24 University Of Southern California Method of promoting production of living tissue equivalents
US20050180958A1 (en) * 1999-02-04 2005-08-18 Technion Research & Development Foundation Ltd. Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
US20050112759A1 (en) * 2003-06-20 2005-05-26 Milica Radisic Application of electrical stimulation for functional tissue engineering in vitro and in vivo

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OGATA ET AL.: "Identification and Hematopoietic Potential of CD45? Clonal Cells with Very Immature Phenotype (CD45?CD34?CD38?Lin?) in Patients with Myelodysplastic Syndromes", STEM CELLS., vol. 23, 2005, pages 619 - 630 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9198938B2 (en) 2008-11-19 2015-12-01 Antrhogenesis Corporation Amnion derived adherent cells

Also Published As

Publication number Publication date
WO2008152640A2 (fr) 2008-12-18

Similar Documents

Publication Publication Date Title
WO2008152640A3 (fr) Echafaudages biocompatibles tridimensionnels pour l'expansion ex-vivo et la transplantation de cellules souches
WO2007091255A3 (fr) Méthode et dispositif de maintien et d'expansion de cellules souches hématopoïétiques à partir de cellules mononuclées
WO2009028631A1 (fr) Agent pour favoriser l'adhérence cellulaire endothéliale cornéenne
RU2011124900A (ru) Культура плюрипотентных стволовых клеток на микроносителях
NZ602455A (en) Amnion derived adherent cells
WO2010147395A3 (fr) Composition de milieu comprenant un neuropeptide y pour la génération, le maintien, la croissance non différenciée prolongée de cellules souches pluripotentes et procédé de culture de cellules souches pluripotentes l'utilisant
WO2011005773A3 (fr) Procédé de culture de cellules eucaryotes
WO2009016078A3 (fr) Procédé de culture de cellules souches de mammifère
WO2007115337A8 (fr) Ingéniérie tissulaire utilisant des cellules souches embryonnaires humaines
WO2009063296A3 (fr) Systèmes et procédés pour la fabrication de biocarburant
DK3633025T3 (da) Dyrkningsmedier, cellekulturer og metoder til dyrkning af pluripotente stamceller i en udifferentieret tilstand
MXPA01007820A (es) Metodo y aparato para mantenimiento y expansion de celulas de tallo hemopoyeticas y/o celulas progenitoras.
SG150567A1 (en) Pluripotent stem cell growing method
WO2011008986A3 (fr) Procédé d'application de forçage hémodynamique et klf2 pour initier la croissance et le développement de valvules cardiaques
WO2006010117A3 (fr) Production de flavonoides par micro-organismes recombines
NZ611186A (en) Fermentation process involving adjusting specific co-uptake
WO2008102572A1 (fr) Procédé de fabrication d'acide aminé l ou d'acide nucléique
NZ591812A (en) Cell culture method to form aggregates
WO2007143117A3 (fr) Différentiation de cellules pluripotentes de primates en cellules de lignées hépatocytaires
WO2006130651A3 (fr) Methode d'elaboration de cellules dendritiques a partir de cellules souches embryonnaires
NO20064379L (no) Anvendelse av et serumfritt cellekulturmedium for produsjonen av IL-18BP i pattedyrceller
WO2013141731A8 (fr) Procédé d'expansion ex vivo de cellules souches dans un bioréacteur
WO2003069972A3 (fr) Lignees cellulaires precurseurs d'hepatocytes
WO2006135673A3 (fr) Production de gaz hydrogene et isolation de micro-organismes produisant l'hydrogene a l'aide de substrats recouverts par regeneration
MX2010000496A (es) Cultivo para expandir citoblastos ex-vivo.

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 08763561

Country of ref document: EP

Kind code of ref document: A2

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 08763561

Country of ref document: EP

Kind code of ref document: A2