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WO2008144791A3 - Verfahren zum umwandeln von xylose oder xylose enthaltenden substratlösungen - Google Patents

Verfahren zum umwandeln von xylose oder xylose enthaltenden substratlösungen Download PDF

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Publication number
WO2008144791A3
WO2008144791A3 PCT/AT2008/000184 AT2008000184W WO2008144791A3 WO 2008144791 A3 WO2008144791 A3 WO 2008144791A3 AT 2008000184 W AT2008000184 W AT 2008000184W WO 2008144791 A3 WO2008144791 A3 WO 2008144791A3
Authority
WO
WIPO (PCT)
Prior art keywords
xylose
ser
converting
ethanol
yeast strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/AT2008/000184
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English (en)
French (fr)
Other versions
WO2008144791A2 (de
Inventor
Barbara Petschacher
Bernd Nidetzky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vogelbusch GmbH
Original Assignee
Vogelbusch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vogelbusch GmbH filed Critical Vogelbusch GmbH
Publication of WO2008144791A2 publication Critical patent/WO2008144791A2/de
Publication of WO2008144791A3 publication Critical patent/WO2008144791A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft ein Verfahren zum Umwandeln von Xylose oder Xylose enthaltenden Substraten, z.B. Hydrolysaten von Lignocellulosen in Ethanol mittels Saccharomyces cerevisiae. Um die Ausbeute an Ethanol zu erhöhen und die Produktion von Nebenprodukten zu vermindern, wird zur Umwandlung ein Hefestamm eingesetzt, welcher eine an sich bekannte Mutante der pilzlichen Xylose-Reduktase, in der ein Sequenzmotiv Lys-Ser-Asn (homolog zu Lys274-Ser-Asn276 in Xylose Reduktase aus Candida tenuis) gegen Arg-Ser-Asp ausgetauscht wurde, und den Wildtyp der Xylit-Dehydrogenase von z.B. Galactocandida mastrotermitis, exprimiert, wobei der Hefestamm gegebenenfalls wenigstens eine zusätzliche Genkopie des Gens für Xylulose-Kinase enthält.
PCT/AT2008/000184 2007-05-30 2008-05-29 Verfahren zum umwandeln von xylose oder xylose enthaltenden substratlösungen Ceased WO2008144791A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
ATA849/2007 2007-05-30
AT0084907A AT505251B1 (de) 2007-05-30 2007-05-30 Verfahren zum umwandeln von xylose oder xylose enthaltenden substratlísungen

Publications (2)

Publication Number Publication Date
WO2008144791A2 WO2008144791A2 (de) 2008-12-04
WO2008144791A3 true WO2008144791A3 (de) 2009-02-05

Family

ID=39968103

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/AT2008/000184 Ceased WO2008144791A2 (de) 2007-05-30 2008-05-29 Verfahren zum umwandeln von xylose oder xylose enthaltenden substratlösungen

Country Status (2)

Country Link
AT (1) AT505251B1 (de)
WO (1) WO2008144791A2 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0921734A2 (pt) 2008-10-31 2019-01-08 California Inst Of Techn micro-organismos criados geneticamente capazes de produzir compostos-alvo sob condições anaeróbicas
EP3074504B1 (de) 2013-11-25 2024-12-25 Genomatica, Inc. Verfahren zur verbesserung der mikrobiellen herstellung von fettalkoholen mit bestimmter länge in gegenwart von methanol
SI3077501T1 (sl) 2013-12-03 2022-02-28 Genomatica, Inc. Mikroorganizmi in metode za izboljšanje donosa izdelkov z uporabo sinteze acetil-coa

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005093041A1 (en) * 2004-03-26 2005-10-06 Forskarpatent I Syd Ab Mutated xylose reductase in xylose-fermentation by s. cerevisiae

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005093041A1 (en) * 2004-03-26 2005-10-06 Forskarpatent I Syd Ab Mutated xylose reductase in xylose-fermentation by s. cerevisiae

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHU BYRON C H ET AL: "Genetic improvement of Saccharomyces cerevisiae for xylose fermentation", BIOTECHNOLOGY ADVANCES, vol. 25, no. 5, 24 April 2007 (2007-04-24), pages 425 - 441, XP002504767, ISSN: 0734-9750 *
HABENICHT ANDREAS ET AL: "Xylose utilisation: Cloning and characterisation of the xylitol dehydrogenase from Galactocandida mastotermitis", BIOLOGICAL CHEMISTRY, vol. 380, no. 12, December 1999 (1999-12-01), pages 1405 - 1411, XP009108937, ISSN: 1431-6730 *
NIDETZKY BERND ET AL: "Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli.", CHEMICO-BIOLOGICAL INTERACTIONS, vol. 143-144, 1 February 2003 (2003-02-01), pages 533 - 542, XP002504770, ISSN: 0009-2797 *
PETSCHACHER B ET AL: "ENGINEERING CANDIDA TENUIS XYLOSE REDUCTASE FOR IMPROVED UTILIZATION OF NADH: ANTAGONISTIC EFFECTS OF MULTIPLE SIDE CHAIN REPLACEMENTS AND PERFORMANCE OF SITE-DIRECTED MUTANTS UNDER SIMULATED IN VIVO CONDITIONS", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 71, no. 10, 1 October 2005 (2005-10-01), pages 6390 - 6393, XP008054328, ISSN: 0099-2240 *
PETSCHACHER BARBARA ET AL: "Altering the coenzyme preference of xylose reductase to favor utilization of NADH enhances ethanol yield from xylose in a metabolically engineered strain of Saccharomyces cerevisiae", MICROBIAL CELL FACTORIES, BIOMED CENTRAL, LONDON, NL, vol. 7, no. 1, 17 March 2008 (2008-03-17), pages 9, XP021036443, ISSN: 1475-2859 *
PETSCHACHER BARBARA ET AL: "The coenzyme specificity of Candida tenuis xylose reductase (AKR2B5) explored by site-directed mutagenesis and X-ray crystallography", BIOCHEMICAL JOURNAL, vol. 385, no. Part 1, January 2005 (2005-01-01), pages 75 - 83, XP002504768, ISSN: 0264-6021 *
WATANABE SEIYA ET AL: "The positive effect of the decreased NADPH-preferring activity of xylose reductase from Pichia stipitis on ethanol production using xylose-fermenting recombinant Saccharomyces cerevisiae", BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, vol. 71, no. 5, May 2007 (2007-05-01), pages 1365 - 1369, XP002504769, ISSN: 0916-8451 *

Also Published As

Publication number Publication date
AT505251A1 (de) 2008-12-15
WO2008144791A2 (de) 2008-12-04
AT505251B1 (de) 2009-09-15

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