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WO2008019183A3 - Biopolymer and protein production using type iii secretion systems of gram negative bacteria - Google Patents

Biopolymer and protein production using type iii secretion systems of gram negative bacteria Download PDF

Info

Publication number
WO2008019183A3
WO2008019183A3 PCT/US2007/069300 US2007069300W WO2008019183A3 WO 2008019183 A3 WO2008019183 A3 WO 2008019183A3 US 2007069300 W US2007069300 W US 2007069300W WO 2008019183 A3 WO2008019183 A3 WO 2008019183A3
Authority
WO
WIPO (PCT)
Prior art keywords
type iii
negative bacteria
gram negative
biopolymer
iii secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2007/069300
Other languages
French (fr)
Other versions
WO2008019183A2 (en
Inventor
Christopher Ashby Voigt
Daniel Matthew Widmaier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of WO2008019183A2 publication Critical patent/WO2008019183A2/en
Anticipated expiration legal-status Critical
Publication of WO2008019183A3 publication Critical patent/WO2008019183A3/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Proteins, polynucleotide, expression cassette, vector and bacterium compositions for obtaining proteins of interest by expression of same in gram negative bacteria having a Type III secretion system are provided. Uses of the proteins so obtained in the manufacture of isolated proteins, silks, fibers threats, and pharmaceutical compositions are also provided.
PCT/US2007/069300 2006-05-18 2007-05-18 Biopolymer and protein production using type iii secretion systems of gram negative bacteria Ceased WO2008019183A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80175806P 2006-05-18 2006-05-18
US60/801,758 2006-05-18

Publications (2)

Publication Number Publication Date
WO2008019183A2 WO2008019183A2 (en) 2008-02-14
WO2008019183A3 true WO2008019183A3 (en) 2008-12-04

Family

ID=39033539

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/069300 Ceased WO2008019183A2 (en) 2006-05-18 2007-05-18 Biopolymer and protein production using type iii secretion systems of gram negative bacteria

Country Status (1)

Country Link
WO (1) WO2008019183A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015078840A1 (en) * 2013-11-26 2015-06-04 Boehringer Ingelheim International Gmbh Full and partial protein secretion and cell surface display using type iii secretion system
KR102648293B1 (en) * 2014-05-21 2024-03-15 우니페르시테트 바젤 Bacteria-based protein delivery
HRP20211191T1 (en) 2015-11-19 2021-10-29 Universität Basel Virulence attenuated bacteria for treatment of malignant solid tumors
WO2017087827A1 (en) * 2015-11-19 2017-05-26 President And Fellows Of Harvard College Method of making recombinant silk andsilk-amyloid hybrid proteins using bacteria
US12351805B2 (en) 2015-11-19 2025-07-08 Northeastern University Method of making recombinant silk and silk-amyloid hybrid proteins using bacteria
PT3377634T (en) * 2015-11-19 2020-11-16 Univ Basel Bacteria-based protein delivery
CA3046852A1 (en) 2016-12-20 2018-06-28 Universitat Basel Virulence attenuated bacteria based protein delivery
WO2018132821A2 (en) * 2017-01-13 2018-07-19 Bolt Threads, Inc. Elastomeric proteins
WO2019090267A1 (en) * 2017-11-03 2019-05-09 The Regents Of The University Of California Methods and compositions useful for inhibiting growth of certain bacteria
US11178934B2 (en) 2018-07-18 2021-11-23 Bolt Threads Inc. Resilin material footwear and fabrication methods
EP3932937A1 (en) * 2020-07-03 2022-01-05 Universitätsklinikum Hamburg-Eppendorf Novel protein translocation domain
WO2023076924A1 (en) 2021-10-26 2023-05-04 Rensselaer Polytechnic Institute Systems and methods for increased production of recombinant biopolymers via genome engineering and downregulation of basal expression

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DARWIN ET AL.: "The putative invasion proteiin chaperone SicA acts together with InvF to activate the expression of Salmonella typhimurium virulence genes", MOL. MICROBIOL., vol. 35, no. 4, February 2000 (2000-02-01), pages 949 - 960 *
GUZMAN ET AL.: "Direct expression of Bordetella pertussis filamentous hemagglutinin in Escherichia coli and Salmonella typhimurium aroA", INFECT. IMMUN., vol. 59, no. 10, October 1991 (1991-10-01), pages 3787 - 3795 *
WINSTANLEY ET AL.: "Type III secretion systems and pathogenicity islands", J. MED. MICROBIOL., vol. 50, no. 2, February 2001 (2001-02-01), pages 116 - 126, XP002580287 *

Also Published As

Publication number Publication date
WO2008019183A2 (en) 2008-02-14

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