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WO2008064730A3 - Verfahren zum durchführen einer enzymatischen reaktion - Google Patents

Verfahren zum durchführen einer enzymatischen reaktion Download PDF

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Publication number
WO2008064730A3
WO2008064730A3 PCT/EP2007/008123 EP2007008123W WO2008064730A3 WO 2008064730 A3 WO2008064730 A3 WO 2008064730A3 EP 2007008123 W EP2007008123 W EP 2007008123W WO 2008064730 A3 WO2008064730 A3 WO 2008064730A3
Authority
WO
WIPO (PCT)
Prior art keywords
reaction
carrying
eukaryotic cell
enzymatic reaction
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/008123
Other languages
English (en)
French (fr)
Other versions
WO2008064730A2 (de
Inventor
Christoph Gauer
Wolfgang Mann
Marianna Alunni-Fabbroni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Advalytix AG
Original Assignee
Advalytix AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advalytix AG filed Critical Advalytix AG
Priority to US12/312,906 priority Critical patent/US20110081684A1/en
Priority to EP07818225A priority patent/EP2089547A2/de
Priority to JP2009538597A priority patent/JP2010510782A/ja
Publication of WO2008064730A2 publication Critical patent/WO2008064730A2/de
Publication of WO2008064730A3 publication Critical patent/WO2008064730A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Ein Verfahren zum Durchführen einer enzymatischen Reaktion, insbesondere zum Durchführen einer PCR, umfasst die Entnahme wenigstens einer Eukaryontenzelle aus einem Ausgangsmaterial, das Anfärben des Zellkerns bzw. der Zellkerne der Eukaryontenzelle(n), das Ablegen wenigstens einer Eukaryontenzelle auf einer Reaktionsstelle eines festen Substrats in einem Flüssigkeitsvolumen von weniger als 10 μl, das Detektieren, ob wenigstens ein gefärbter Zellkern auf einer Reaktionsstelle des Substrats vorliegt, die anschließende Zugabe von Enzym und gegebenenfalls Reaktionspuffer zu der/ den Eukaryontenzelle(n) sowie abschließend den Start der enzymatischen Reaktion. Vorzugsweise wird das erfindungsgemäße Verfahren unter Einsatz eines Durchflusszytometers durchgeführt.
PCT/EP2007/008123 2006-11-30 2007-09-18 Verfahren zum durchführen einer enzymatischen reaktion Ceased WO2008064730A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/312,906 US20110081684A1 (en) 2006-11-30 2007-09-18 Method for carrying out an enzymatic reaction
EP07818225A EP2089547A2 (de) 2006-11-30 2007-09-18 Verfahren zum durchführen einer enzymatischen reaktion
JP2009538597A JP2010510782A (ja) 2006-11-30 2007-09-18 酵素反応を実行するための方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102006056694.7 2006-11-30
DE102006056694A DE102006056694B4 (de) 2006-11-30 2006-11-30 Verfahren zum Durchführen einer enzymatischen Reaktion

Publications (2)

Publication Number Publication Date
WO2008064730A2 WO2008064730A2 (de) 2008-06-05
WO2008064730A3 true WO2008064730A3 (de) 2008-07-24

Family

ID=39032321

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/008123 Ceased WO2008064730A2 (de) 2006-11-30 2007-09-18 Verfahren zum durchführen einer enzymatischen reaktion

Country Status (5)

Country Link
US (1) US20110081684A1 (de)
EP (1) EP2089547A2 (de)
JP (1) JP2010510782A (de)
DE (1) DE102006056694B4 (de)
WO (1) WO2008064730A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102007059476B3 (de) * 2007-12-11 2009-07-30 Advalytix Ag Vorrichtung und Verfahren zur Vermehrung und zur anschließenden Charakterisierung von Zellen
WO2013145905A1 (ja) 2012-03-30 2013-10-03 ソニー株式会社 微小粒子分取装置及び該装置における流体ストリーム最適化方法
JP5924077B2 (ja) 2012-03-30 2016-05-25 ソニー株式会社 微小粒子分取装置及び微小粒子分取装置における軌道方向判定方法
JP6304034B2 (ja) 2013-01-28 2018-04-04 ソニー株式会社 微小粒子分取装置、微小粒子分取方法及びプログラム
WO2014168043A1 (ja) * 2013-04-09 2014-10-16 東京エレクトロン株式会社 測定装置及び測定方法
EP3035030B1 (de) 2013-10-16 2019-07-10 Sony Corporation Partikelfraktionierungsvorrichtung, partikelfraktionierungsverfahren und programm
JP6136843B2 (ja) 2013-10-17 2017-05-31 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
US10309892B2 (en) 2014-02-13 2019-06-04 Sony Corporation Particle sorting device, particle sorting method, program, and particle sorting system
JP6102783B2 (ja) 2014-02-14 2017-03-29 ソニー株式会社 粒子分取装置、粒子分取方法及びプログラム
JP6657625B2 (ja) 2014-09-05 2020-03-04 ソニー株式会社 液滴分取装置、液滴分取方法及びプログラム
JP6729597B2 (ja) 2015-10-19 2020-07-22 ソニー株式会社 画像処理装置、微小粒子分取装置及び画像処理方法
EP3438644B1 (de) * 2016-03-28 2020-12-23 FUJIFILM Corporation Zellanalysesystem
JP7059712B2 (ja) * 2018-03-14 2022-04-26 株式会社リコー プレートの生成方法、プレートの生成装置、及びオリフィスの径可変プログラム

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064821A2 (de) * 2001-01-30 2002-08-22 Advalytix Ag Verfahren zur analyse von makromolekülen
WO2003012389A2 (de) * 2001-07-24 2003-02-13 Advalytix Ag Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung
WO2003056334A2 (de) * 2001-12-28 2003-07-10 Advalytix Ag Charakterisierungsverfahren für funktionalisierte oberflächen
US20060024272A1 (en) * 2004-07-29 2006-02-02 Large Scale Biology Corporation C-terminally truncated interferon
WO2007059839A1 (de) * 2005-11-28 2007-05-31 Advalytix Ag Verfahren, vorrichtung und kit zur untersuchung von makromolekülen in einer probe

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6540895B1 (en) * 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US20020064809A1 (en) * 2000-11-29 2002-05-30 Mutz Mitchell W. Focused acoustic ejection cell sorting system and method
EP1404875A1 (de) * 2001-06-08 2004-04-07 ChemoMetec A/S Verfahren und system zur zählung von zellen mehrerer spezies

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064821A2 (de) * 2001-01-30 2002-08-22 Advalytix Ag Verfahren zur analyse von makromolekülen
WO2003012389A2 (de) * 2001-07-24 2003-02-13 Advalytix Ag Verfahren zur analyse von makromolekülen, analysevorrichtung und verfahren zur herstellung einer analysevorrichtung
WO2003056334A2 (de) * 2001-12-28 2003-07-10 Advalytix Ag Charakterisierungsverfahren für funktionalisierte oberflächen
US20060024272A1 (en) * 2004-07-29 2006-02-02 Large Scale Biology Corporation C-terminally truncated interferon
WO2007059839A1 (de) * 2005-11-28 2007-05-31 Advalytix Ag Verfahren, vorrichtung und kit zur untersuchung von makromolekülen in einer probe

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"DETECTION OF DNA IN SINGLE CELLS USING AN AUTOMATED CELL DEPOSITION UNIT AND PCR", BIOTECHNIQUES, INFORMA LIFE SCIENCES PUBLISHING, WESTBOROUGH, MA, US, vol. 19, no. 4, 1 January 1995 (1995-01-01), pages 616,618 - 620, XP001015630, ISSN: 0736-6205 *
MANN ET AL.: "Sequenzieren von Einzelzellen in der Routine", BIOSPEKTRUM, April 2007 (2007-04-01), Spektrum Verlag, pages 395 - 396, XP002479983 *
See also references of EP2089547A2 *
WARREN LUIGI ET AL: "Transcription factor profiling in individual hematopoietic progenitors by digital RT-PCR", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 103, no. 47, November 2006 (2006-11-01), pages 17807 - 17812, XP002479982, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
US20110081684A1 (en) 2011-04-07
EP2089547A2 (de) 2009-08-19
DE102006056694B4 (de) 2010-08-05
WO2008064730A2 (de) 2008-06-05
DE102006056694A1 (de) 2008-06-05
JP2010510782A (ja) 2010-04-08

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