[go: up one dir, main page]

WO2007098821A1 - Dispositif d'éclairage multispectral - Google Patents

Dispositif d'éclairage multispectral Download PDF

Info

Publication number
WO2007098821A1
WO2007098821A1 PCT/EP2007/000131 EP2007000131W WO2007098821A1 WO 2007098821 A1 WO2007098821 A1 WO 2007098821A1 EP 2007000131 W EP2007000131 W EP 2007000131W WO 2007098821 A1 WO2007098821 A1 WO 2007098821A1
Authority
WO
WIPO (PCT)
Prior art keywords
radiation
radiation sources
optical
sources
deflection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2007/000131
Other languages
German (de)
English (en)
Inventor
Christian Dietrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jenoptik AG
Carl Zeiss Jena GmbH
Original Assignee
VEB Carl Zeiss Jena GmbH
Carl Zeiss Jena GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VEB Carl Zeiss Jena GmbH, Carl Zeiss Jena GmbH filed Critical VEB Carl Zeiss Jena GmbH
Publication of WO2007098821A1 publication Critical patent/WO2007098821A1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
    • G02B7/18—Mountings, adjusting means, or light-tight connections, for optical elements for prisms; for mirrors
    • G02B7/182—Mountings, adjusting means, or light-tight connections, for optical elements for prisms; for mirrors for mirrors
    • G02B7/1821—Mountings, adjusting means, or light-tight connections, for optical elements for prisms; for mirrors for mirrors for rotating or oscillating mirrors

Definitions

  • the present invention relates to a multi-spectral illumination device, optical devices, in particular imaging, examination, observation and projection devices, with such a lighting device and a method for providing optical radiation.
  • illumination devices An important field of use of illumination devices are imaging devices which are intended to generate images of an object or a sample to be examined. Typical examples of such imaging devices are microscopes and in particular microscopes with a wide-field optics, which images a given region of the sample to be imaged, and not just a small punctiform region of the sample, onto an image plane.
  • the illumination device serves to provide optical radiation in a desired wavelength range for illuminating the object or the sample.
  • optical radiation is meant in particular electromagnetic radiation having wavelengths in the range from the ultraviolet to the infrared part of the spectrum.
  • the switching between different colors or wavelength ranges within milliseconds and a modulation of the intensity of the sample illumination should be possible within microseconds.
  • the sample is irradiated with excitation radiation in a suitable excitation wavelength range or with a suitable excitation spectrum, which is selected as a function of one or more fluorescent dyes. If these fluorescent dyes are present in the sample, they interact with the excitation radiation and emit fluorescence radiation that is characteristic of the fluorescent dye. In this way, a detection of fluorescent dyes in a sample is possible. In this case, not only the presence of fluorescent dyes or substances to which the fluorescent dyes are bound, but also their concentration can be determined.
  • fluorescent dyes are used which specifically bind to predetermined substances in a biological sample and can then be detected in the bound state. It is thus possible to infer the presence and concentration of the given substance in the sample.
  • fluorescence readers are used, which are designed for the investigation of biochips with several, usually even very many, mutually delimited areas, each with a different chemical composition. Such devices are used to examine a large number of samples as possible in an investigation on several different fluorescent dyes.
  • sequential release of optical radiation in at least two different wavelength ranges means that optical radiation is emitted in succession for a given period of time, the intensity of which in each of the wavelength ranges is at least one pronounced maximum, the intensity not necessarily being in the range must disappear between the two wavelength ranges.
  • One possibility for multispectral illumination is the use of white light sources, from the optical radiation of which a desired range is selected.
  • the optical radiation can be spatially split by means of a dispersive element.
  • a desired spectral component of the optical radiation can then be selected.
  • This approach has the disadvantage that a change between different wavelength ranges is expensive.
  • the use of dispersive elements is relatively expensive and expensive and also not very effective in terms of radiation yield.
  • Another possibility is to filter only a certain desired wavelength range, for example by means of a color filter or a dichroic. For changing the wavelength ranges, it is possible, for example, to use filter wheels which, however, due to the moving mass, do not permit a rapid change between the wavelength ranges.
  • a general disadvantage of using a white light source is that the switching on and off as well as a modulation of the intensity can not be done very quickly, since these radiation sources are very sluggish.
  • mechanical closures and gray filters are necessary, which entail a complex construction.
  • Another possibility is to use several colored radiation sources, i. To use sources of optical radiation that emit optical radiation in each different wavelength ranges or with different characteristic wavelength.
  • DE 103 14 125 A1 describes an arrangement for illuminating objects with light of different wavelengths in microscopes, automatic microscopes and devices for fluorescence microscopy applications, comprising LED light sources for object illumination, which are arranged in the illumination beam path of the microscope or device.
  • a receiving device rotatable about an axis of rotation is provided with holders for at least one of the light-emitting diodes.
  • the recording device is adjustable so that the LED can be moved with the respective required for measurements and / or observations focus wavelength in the illumination beam path.
  • this concept has the disadvantage that the rotatable receiving device with light-emitting diodes held therein has a considerable mass, which does not allow a very rapid change between the wavelength ranges readily.
  • the present invention is therefore an object of the invention to provide a lighting device for the sequential emission of optical radiation in each different predetermined wavelength ranges, which is simple in construction and a rapid change of the wavelength range of the radiation emitted by her dust, and a method for Provide optical radiation in at least two different wavelength ranges.
  • an illumination device for emitting optical radiation through at least one decoupling interface with at least two radiation sources for emitting optical radiation in respectively different wavelength ranges
  • at least one optical deflection device for deflection about a predetermined axis relative to the radiation sources the emitted optical radiation and drive means for rotating the deflection means about the axis
  • the radiation sources being disposed relative to the deflection means and the axis such that optical radiation emitted by each of the radiation sources is rotated about the axis in an appropriate angular position or pivoted deflection can be deflected into the decoupling interface.
  • the object is further achieved by a method for the sequential provision of optical radiation in at least two different wavelength ranges in a predetermined time change through at least one decoupling interface, in particular using a lighting device according to the invention, in which with at least two radiation sources, each of which optical radiation in one another of the wavelength ranges, optical radiation is generated in one of the wavelength ranges and directed to an optical deflection device, and the optical deflection is rotated or pivoted relative to the radiation sources so that in the predetermined time change optical radiation of the different wavelength ranges in the Decoupling interface is deflected.
  • the illumination device is therefore intended to emit optical radiation through a decoupling interface.
  • a decoupling interface is understood as meaning a predefined area of the illumination device or a surface positioned fixedly relative to the illumination device, through which optical radiation is emitted in a direction predetermined by at least one part of the illumination device.
  • the direction is understood to mean the direction of the beams of the beam, averaged over the cross section of the emitted radiation beam.
  • the radiation sources are designed to emit optical radiation in respectively different wavelength ranges, ie they each have different emission spectra. This is understood to mean that the characteristic wavelengths of the respective emission spectra, for example intensity maxima of the respective emission spectra or heavy point wavelength of the respective emission spectra or their dominant wavelengths, from each other, preferably spaced by more than 50 nm.
  • emission peaks of the radiation sources do not overlap at least within the half-value width of the peaks, or only in the flanks of the emission peaks.
  • the radiation sources emit optical radiation of different "color”.
  • optical radiation is emitted by the corresponding radiation source in the direction of the deflection device.
  • the deflection device is brought into a position by turning or pivoting in which the optical radiation of the selected radiation source is deflected by the deflection device into the decoupling interface.
  • the movement of the deflection device takes place in the device by means of the drive device, which can for this purpose comprise an actuator or actuator which can be controlled by control signals.
  • the lighting device is characterized by a very simple structure and in particular does not need to have moving mechanical components with large mass or large moment of inertia, since optical deflection can be very light and already rotation around a very small angle can be sufficient to instead of radiation first of the radiation sources to direct radiation of a second of the radiation sources in the decoupling interface. This allows a very fast change between the wavelength ranges.
  • the radiation sources need not be moved, in principle any radiation sources can be used which can deliver optical radiation in the desired wavelength ranges.
  • the radiation sources comprise at least one radiation source whose radiation is emitted to the deflection device via fiber optics.
  • a radiation source whose radiation is emitted to the deflection device via fiber optics is then preferably used as at least one of the radiation sources. This allows in particular to use even bulky radiation sources whose optical radiation can then be brought by the fiber optics in a spatially small area near the deflection device.
  • the radiation sources preferably comprise at least one laser.
  • a laser is preferably used as at least one of the radiation sources. This has the advantage that through the laser Particularly intense and / or narrowband and / or coherent radiation can be delivered compared to other radiation sources.
  • the radiation sources particularly preferably comprise at least one semiconductor radiation source.
  • a semiconductor radiation source is then preferably used as at least one of the radiation sources.
  • This embodiment has a number of advantages. So build semiconductor radiation sources very compact, so that the lighting device itself can also be made compact. Further, there are available semiconductor radiation sources which provide optical radiation in narrow wavelength ranges, i. emitted colored optical radiation. By choosing appropriate semiconductor radiation sources very different wavelength ranges can be covered. Compared to conventional light sources such as arc or halogen lamps, the use of semiconductor radiation sources, in particular light emitting diodes, moreover has the advantage that they have a much longer life. Furthermore, the heat generation by semiconductor radiation sources during their operation is much lower than with arc or halogen lamps. An active cooling of the semiconductor radiation sources can therefore be omitted, a passive cooling can be significantly simplified compared to the use of arc or halogen lamps. Preferably, all radiation sources are semiconductor radiation sources.
  • the semiconductor radiation sources may be any semiconductor devices emitting optical radiation.
  • laser diodes or superluminescent diodes can be used.
  • light-emitting diodes or light emitting diodes or organic light-emitting diodes are used.
  • high-performance light-emitting diodes which emit optical radiation of high intensity.
  • powerful colored light-emitting diodes are used for the lighting device.
  • light-emitting diodes with applied phosphors for color conversion which emit optical radiation in another wavelength range excited by optical radiation emitted by the semiconductor material of the light-emitting diodes, can also be used.
  • semiconductor radiation sources in particular light-emitting diodes, have the advantage that their emission spectra are broadband than many lasers, so that it is easier to filter out a certain desired proportion by means of suitable filter selection. Also, the problems that occur in ordinary lasers due to the high coherence of the laser radiation, such as speckle formation, do not occur or not so pronounced.
  • the light-emitting diodes may in particular be those having a plane surface from which the optical radiation emerges or light-emitting diodes having a transparent dome arranged above the surface for reducing the refractive index jump to the semiconductor material. your.
  • the use of four semiconductor radiation sources allows good coverage of a given spectral range, in particular the entire optical spectrum, in particular the visible spectrum as well as the UV and NIR range.
  • at least one of the semiconductor radiation sources is preferably a UV or NIR radiation source with a characteristic emission wavelength in the UV or NIR range.
  • a field of the same light emitting diodes can be used.
  • the illumination device comprises at least one semiconductor radiation source with at least one phosphor for color conversion, in the beam path of which a band-pass filter is arranged and whose radiation is likewise emitted to the deflection device.
  • the bandpass filter is preferably located between a collimation device associated with the semiconductor radiation source and the semiconductor radiation source itself.
  • a color conversion by a phosphor is understood to mean that the phosphor emitted by a radiation-emitting layer of the semiconductor radiation source by fluorescence and / or phosphorescence in radiation in the desired wavelength range, ie Radiation of other color, converted.
  • the bandpass filter is chosen so that the radiation of the semiconductor radiation source is limited spectrally to a suitable wavelength range. Preferably, that range is in the range of 570 nm.
  • This embodiment has the advantage that gaps in the wavelength range of the optical spectrum that can be covered by semiconductor radiation sources can be filled by using a corresponding bandpass filter.
  • the radiation sources can be arranged as desired, as long as their radiation can be deflected by the deflection in a suitable position in the decoupling interface.
  • the radiation sources are arranged in one plane.
  • the axis about which the deflector can be pivoted or rotated is orthogonal to the plane.
  • the radiation sources are arranged, for example, on a circuit board.
  • the adjustment of the arrangement of the radiation sources relative to the deflection device or the axis is particularly simple.
  • the radiation sources are arranged on a circular arc.
  • the pivoting or pivoting axis can then run through the center of the circular arc orthogonal to the plane spanned by the circular arc.
  • At least one collimation device for collimating the radiation emitted by the radiation source and / or a filter for limiting the spectrum of the radiation impinging on the deflection device can be provided in the beam path between at least one of the radiation sources and the deflection device , For example, a narrow bandpass filter, be arranged.
  • the collimation has the advantage that on the one hand a collimated beam can be deflected defined and less losses than a divergent, and that on the other hand, the resulting at collimation at least quasi-parallel beam allows the use of interference filters that beso ⁇ % of it precise Dust filtering.
  • the collimation device may comprise at least one concentrator or a holographic or diffractive element which deadens a collimation of the optical radiation emitted by the respective radiation source.
  • at least one of the collimating means comprises an aspherical lens or an aspherical mirror.
  • This embodiment of the collimation device has the advantage that the aspherical design permits a particularly good bundling of the optical radiation emitted by the respective radiation source, in particular the semiconductor radiation source, and thus radiation losses can be reduced by a less directed radiation of the optical radiation from the semiconductor radiation source.
  • the filter serves to mask out long spectral outlets of the emission spectra of the radiation sources, in particular of the semiconductor radiation sources, and thus to possibly avoid disturbing background signals. This has the advantage that a later evaluation in a fluorescence examination is facilitated.
  • the bandpass filter preferably has a spectral width of less than 100 nm.
  • the deflection device has at least one deflection element which deflects radiation incident thereon.
  • a deflection is generally understood to mean that radiation incident from one direction is generated in a different direction, not only diffusely scattered radiation.
  • the deflection device can therefore preferably comprise at least one of the following deflection elements or also a combination of at least two of these deflection elements.
  • the deflection device of the device can have a prism as deflecting element.
  • the deflection device as deflecting a diffractive element having. This can in particular be a diffraction grating.
  • the deflection device has at least one mirror as a deflecting element.
  • the use of a mirror has the advantage that a very high proportion of the incident radiation is actually deflected and the deflection takes place largely independently of the wavelength of the incident radiation.
  • mirrors can have a very low moment of inertia, so that a fast rotation is possible.
  • the drive device For the movement of the deflection device, the drive device is used, which can have an actuator which can be controlled via control signals or an actuator which can be controlled via control signals, for example an electric motor or a galvano drive, by means of which the deflection device can be placed in a position corresponding to the control signal.
  • the device then preferably also comprises a control device for activating the drive device in order to move the deflection device into at least two positions in which these optical radiation in each case redirects another of the radiation sources into the outcoupling section parts.
  • control device is designed to control the drive device. Then, the radiation sources can be operated simultaneously, the selection of wavelength ranges then takes place solely by movement of the deflection. However, it is preferred that the control device is also designed to control the radiation sources.
  • control device may be further configured and connected to the radiation sources, that by means of the control device, the intensity and / or radiation duration of the emitted from the radiation sources optical radiation for at least two of the radiation sources is separated from each other adjustable.
  • the intensity and / or emission duration of the optical radiation emitted by the radiation sources can then be set separately for at least two of the radiation sources.
  • the control device can have corresponding operating elements to be operated by a user, for example switches.
  • a control input is provided, by means of which the control device can receive control signals to which the control device switches on and off one or more of the radiation sources and / or the deflection device by means of disconnection.
  • the radiation sources can be switched on and off individually or in combination.
  • the control device is further configured such that it synchronously or synchronously turns on one of the radiation sources and rotates or swings the deflection device into a position by emitting signals to the drive device in which this optical radiation of the switched radiation source deflects to the coupling-out interface.
  • one of the radiation sources is switched on and the deflection device is rotated or swiveled into a position in which this optical radiation of the switched-on radiation source deflects onto the coupling-out interface.
  • synchronized switching is understood in particular that synchronization can be done by appropriate external synchronization signals.
  • the adjustment of the position of the radiation sources and the decoupling interface and the positions of the deflection device to each other can be done for example by mechanical means.
  • the control device is further configured such that the position of the deflection device for the deflection of the optical radiation of one of the radiation sources to the decoupling interface is electronically adjustable.
  • the Justiertownkeit needs to be possible only in a predetermined range by a mean angle at which the radiation of the radiation source would ideally be directed into the decoupling interface.
  • a homogenization device is preferably arranged in the beam path between the deflection device and the decoupling interface.
  • the radiation to be emitted is preferably homogenized before it reaches the decoupling interface.
  • transparent rods or hollow rods with reflective side walls, diffractive optical elements or diffusing disks can be used as the homogenizing device.
  • the use of bars with reflective walls has the advantage that the losses therein are particularly low.
  • the control device for activating the drive device may be designed to emit signals to the drive device, so that these divert the deflection device into periodic or random movements a middle position offset in which this deflects the radiation emitted by the radiation source optical radiation in the decoupling interface.
  • the deflection device is offset in periodic or statistical movements about a middle position in which it deflects the optical radiation emitted by the radiation source into the decoupling interface.
  • This embodiment has the advantage that not necessarily further optical components need to be arranged in the beam path between the deflection device and the decoupling interface.
  • the deflection does not need to be slowed down to rest, so that not only a homogenization, but also a faster switching can be achieved.
  • the lighting device is generally suitable for lighting purposes requiring rapid variation of the color spectrum.
  • the invention is therefore in particular also an optical device with a lighting device according to the invention.
  • the optical device has, in addition to the illumination device, at least one further optical component which is arranged in the beam path downstream of the coupling-out interface.
  • the illumination device is used to examine samples.
  • the present invention therefore also relates to an optical device for examining a sample, in particular a wide-field microscope or a fluorescence reader, with a lighting device according to the invention.
  • Such an inspection device is characterized by the fact that it has only a few moving parts due to the design of the lighting device and is thus inexpensive and easy to produce. In addition, it can be built very compact.
  • the lighting device is suitable for all types of microscopes. These include e.g. Universal microscopes, optical readers (including biochip readers, titer plate readers), stereo microscopes, surgical microscopes and ophthalmic devices.
  • the examination device can preferably be designed to carry out fluorescence investigations on a sample.
  • FIG. 1 is a schematic representation of a microscope with a lighting device according to a first preferred embodiment of the invention
  • FIG. 2 is a schematic representation of a microscope with a lighting device according to a third preferred embodiment of the invention.
  • Fig. 3 is a schematic representation of a lighting device according to another preferred embodiment of the invention.
  • a microscope 1 in the example a wide field fluorescence microscope, with a lighting device 2 for the examination of a sample 3 is used.
  • the microscope 1 which is only shown very schematically, is designed in a conventional manner and in particular has a coupling-in optical system 4, via which optical radiation for illuminating the sample 3 can be coupled into the illumination beam path of the microscope 1.
  • a coupling-in optical system 4 via which optical radiation for illuminating the sample 3 can be coupled into the illumination beam path of the microscope 1.
  • a coupling-in interface of the microscope 1 is formed which at the same time also forms a decoupling interface of the lighting device 2.
  • the illumination device 2 comprises several, in the example four, radiation sources 6 for emitting optical radiation in respective different wavelength ranges in outgoing beam sources 6 partial beam paths, respectively arranged in the partial beam paths after the radiation sources 6 Kollirhations wornen 7 and 8 and excitation bandpass filter and a arranged in the intersection of the partial beam paths deflection 9 with a deflecting element in the form of a mirror 10 rotatable about an axis for deflecting the incident on the deflector 9 radiation of the radiation sources 6, and a drive means 11 for rotating the deflector 9 about the axis 10.
  • the optical radiation deflected by the deflecting device 8 focussed in a homogenizing device 13, from which the optical radiation in the Auskoppelroughstelle tr Itt, which is given by the exit surface of the homogenizer 13 and the optical axis of the optics 12 and the homogenizer 13.
  • the radiation sources 6 are semiconductor radiation sources, more precisely light-emitting diodes for emitting optical radiation in respectively different wavelength ranges or with different characteristic emission wavelengths, ie in each case different color, in the example red, green, blue or ultraviolet.
  • the characteristic emission wavelength of the respective emission spectrum of one of the semiconductor radiation sources in this exemplary embodiment, as well as in the following exemplary embodiments, is the wavelength with the maximum emission intensity.
  • the LEDs 6 therefore emit, expressed in terms of the color of the characteristic emission wavelength, optical radiation in spectrums of decreasing characteristic wavelength, ie clockwise in Fig. 1 the first LED in the red, the second LED in the green, the third LED in the blue and the fourth LED in the ultraviolet.
  • the light-emitting diodes are chosen so that the emission spectra are well suited to excite at least four predefined fluorescent dyes distributed over the optical spectrum from the UV to the red light.
  • the radiation sources 6 are arranged on a in Fig. 1 indicated by a dashed line circle or arc and thus in a plane defined by the arc.
  • one of the collimation devices 7 is arranged in the partial beam path of each of the semiconductor radiation sources 6 and collimates the optical radiation emitted by the respective semiconductor radiation source 6.
  • aspheric lenses of high numerical aperture preferably greater than 0.5
  • the collimation devices 7 and the optics 12 are further designed and arranged such that the largest possible proportion of the optical radiation emitted by each of the semiconductor radiation sources 6 is coupled into the homogenization device 13.
  • the collimation devices 7 and the optics 12 are designed in the common beam path section in front of the homogenizing device 13 so that the light conductance values are optimally matched to one another.
  • the trained as interference filter, only optional excitation bandpass filter 8 have each adapted to the emission spectra of the semiconductor radiation sources 6 transmission bands that narrow the spectral width of the emission spectra of the semiconductor radiation sources 6 and thus prevent crosstalk in various simultaneous fluorescence studies for different fluorescent dyes.
  • the deflection device 9, which comprises only a mirror as deflecting element in this exemplary embodiment, is fastened to the shaft or shaft 11 oriented orthogonally to the plane in which the radiation sources 6 are arranged and can be rotated or pivoted by rotation of the shaft 11.
  • the shaft 10 and the deflecting device 9 are arranged in the overlapping region of the partial beam paths behind the radiation sources 6, that in appropriate angular positions of the deflecting device 9 or for appropriate rotation angle of the shaft 10, each optical radiation of one of the radiation sources 6 deflected to the optics 12 can be.
  • the optical system 12 couples the optical radiation impinging on it into the homogenizing device 13 in the form of a mirrored hollow rod, which is aligned with its longitudinal axis parallel to the optical axis of the optical system 12.
  • the drive device 11 For rotation of the deflecting device 9, the drive device 11 is provided, which for this purpose has a controllable by electrical control signals in its position, not shown in the figures, electromagnetic actuator.
  • the control device 14 is for driving the radiation sources 6 independently of one another and for driving the drive means 11 and thus the setting of the deflection device 9 is formed, so that a preferred first embodiment of a method according to the invention can be performed.
  • control device 14 has a user interface (not shown in the figures) via which a user can determine the sequence of the wavelength ranges to be set for the optical radiation to be emitted by the illumination device, its desired intensity and the respective duration of the emission.
  • corresponding data can either be read in or the user has the option of selecting one from a predetermined number of combinations.
  • the order, the intensities and the radiation durations may also be fixed.
  • the duty cycle of the respective radiation source and / or the on and / or off timing may be controlled and / or synchronized by at least one signal from an external device connected to the controller 14 via at least one sync or trigger signal connection.
  • control device 14 has a user interface, by means of which adjustment inputs can be detected. These inputs are used to adjust the angular position of the deflection or the shaft 11 for each of the radiation sources 6 by electrical signals so that an at least approximately optimal coupling of the deflected optical radiation in the optics 12 and the homogenizer 13 and thus the decoupling interface is possible.
  • a user enters for this purpose in an adjustment mode of the control device 14 for each of the radiation sources 6 separately adjustment signals in the control device 14, to which this turns by delivering corresponding control signals by means of the drive means 11, the deflection device 9.
  • the corresponding position of the shaft 11 is stored in the control device 14 in the form of appropriate control data associated with the radiation source. Upon selection of one of the radiation sources 6 for illumination, these data are then used to control the drive device 11.
  • the control device 14 switches on the respective radiation source 6 and shuts off, as far as this is still the case, radiation sources still in operation, so that optical radiation is only switched on - Th emitted radiation source, is collimated by the corresponding collimation 7 and then spectrally filtered by the corresponding excitation bandpass filter 8.
  • the control device 14 synchronously or synchronized to the output of the signals for the radiation sources 6 setting signals to the drive device 11 so that it turns the deflecting device 9 in the selected, the switched radiation source 6 corresponding angular position in which the deflection device 9 the collimated and filtered optical radiation incident thereon from the selected switched-on radiation source 6 is directed in a direction parallel to the optical axis of the focusing optic 12 onto the focusing optic 12, which couples the deflected radiation into the homogenizer 13.
  • the radiation source 6 is first turned on and thus the desired radiation output is started after the deflecting device 9 has been rotated into the angular position corresponding to the selected radiation source 6 and the radiation sources 6 are switched off during one rotation of the deflecting device 9 into one of the angular positions.
  • the intensity of the optical radiation can be adjusted by appropriate activation of the radiation sources 6 for each selected wavelength range or each switched radiation source separately, ie, regardless of the choice of the intensities of the delivered in other wavelength ranges optical radiation, be adjusted by appropriate signals of the controller 14.
  • the duration of the emission of optical radiation or the frequency with which the wavelength ranges can be changed can, depending on the activation and selection of the actuator, be in the range of milliseconds.
  • a second preferred embodiment differs from the first exemplary embodiment in that the control device is designed to modulate the intensity of the emitted radiation on a time scale of microseconds during the emission of optical radiation by one of the radiation sources by corresponding control of the radiation source, which is particularly is possible by the use of light-emitting diodes as radiation sources without the use of mechanical fasteners.
  • a third preferred embodiment of the illumination device illustrated in FIG. 2 differs from the first embodiment only in that only two radiation sources in the form of light-emitting diodes are used, which are arranged on a circular arc in a plane orthogonal to the optical axis of FIG Optics 12 and the homogenization device 13 and thus the decoupling interface are arranged.
  • the control device 14 is designed to control only the two radiation sources.
  • the axis or shaft 10 is now arranged parallel to the optical axis of the optics 12 and the homogenizing device 13 and thus the decoupling interface. Otherwise, the arrangement corresponds to the arrangement in Fig. 1 and the same reference numerals are used and the comments on the first embodiment also apply here.
  • the optional excitation band filters 8 are not shown in FIG. 2.
  • the deflecting device is hereby set in periodic movements about a mean angular position for the currently switched radiation source, for example the angular position determined in the above-described adjustment, in which it emits the radiation emitted by the radiation source optical radiation deflects into the decoupling interface.
  • the control device 14 is designed accordingly.
  • drive means a galvanometer drive is preferably used.
  • a homogenization of the intensity distribution in the decoupling interface can take place as a function of time.
  • the deflection device has a prism or a diffractive element, for example a blazed grating, instead of the mirror as deflecting element.
  • the radiation sources include fiber optic elements whose radiating surfaces are arranged like the light emitting diodes in the previous examples.
  • Fig. 3 is very schematically a further preferred embodiment of a lighting device shown as a scheme.
  • decoupling interfaces 15 are provided, by means of which an optical multiplexer 16, for example given by the drive device 11 with the shaft 10 and the deflection device 9 of the preceding embodiments in conjunction with a modified control device 14 ", is provided Radiation of one of the radiation sources 6, which are provided for the emission of optical radiation as in the first embodiment, can be dispensed in. In this example, more than four radiation sources 6 are provided, but the number can be arbitrarily greater than 1.
  • optics 12 and a homogenizing device 13 are provided for each of the coupling-out interfaces 15, not shown in FIG. 3, and that the control device 14 'is modified relative to the control device 14, that for each decoupling interface now a corresponding number of angular positions of the deflecting device 9 is stored, which are used in accordance with the currently switched on or be turned on radiation source when using the respective decoupling interface for adjusting the deflecting device 9.

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

Dispositif d'éclairage destiné à délivrer un rayonnement optique à travers au moins une jonction de découplage, comprenant au moins deux sources (6) de rayonnement pour délivrer un rayonnement optique dans des plages de longueurs d'onde à chaque fois différentes, au moins un dispositif (9) de déviation optique du rayonnement optique délivré pouvant tourner ou pivoter autour d'un axe (10) prédéfini par rapport aux sources (6) de rayonnement et un dispositif (11) d'entraînement pour faire tourner ou faire pivoter le dispositif (9) de déviation autour de l'axe (10), les sources (6) de rayonnement étant disposées par rapport au dispositif (9) de déviation et à l'axe (10) de telle sorte que le rayonnement optique délivré par chacune des sources (6) de rayonnement puisse être dévié dans la jonction de découplage par le dispositif (9) de déviation tourné ou pivoté dans une position angulaire appropriée autour de l'axe (10).
PCT/EP2007/000131 2006-02-27 2007-01-09 Dispositif d'éclairage multispectral Ceased WO2007098821A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE200610009053 DE102006009053A1 (de) 2006-02-27 2006-02-27 Multispektrale Beleuchtungsvorrichtung
DE102006009053.5 2006-02-27

Publications (1)

Publication Number Publication Date
WO2007098821A1 true WO2007098821A1 (fr) 2007-09-07

Family

ID=37899981

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/000131 Ceased WO2007098821A1 (fr) 2006-02-27 2007-01-09 Dispositif d'éclairage multispectral

Country Status (2)

Country Link
DE (1) DE102006009053A1 (fr)
WO (1) WO2007098821A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102014110575B4 (de) 2014-07-25 2017-10-12 Leica Microsystems Cms Gmbh Mikroskop und Verfahren zum optischen Untersuchen und/oder Manipulieren einer mikroskopischen Probe

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6079322A (ja) * 1983-10-07 1985-05-07 Furukawa Electric Co Ltd:The 回転ミラ−付フアイバスコ−プ
WO2003021212A1 (fr) * 2001-08-28 2003-03-13 Baylor College Of Medicine Excitation a lignes multiples par impulsions pour detection par fluorescence non chromatisee
DE20319495U1 (de) * 2003-12-16 2004-03-11 Carl Zeiss Jena Gmbh Beleuchtungseinrichtung für ein Mikroskop
DE10252664A1 (de) * 2002-11-11 2004-06-03 Leica Microsystems Wetzlar Gmbh Mikroskop und Verfahren zur Änderung des Lichtflusses in einem Mikroskop
DE10314125A1 (de) * 2003-03-28 2004-10-21 Carl Zeiss Jena Gmbh Anordnung zur Beleuchtung von Objekten mit Licht unterschiedlicher Wellenlänge
EP1512999A2 (fr) * 2003-08-25 2005-03-09 Olympus Corporation Dispositif optique, appareil d'éclairage utilisant le dispositif, et projecteur utilisant l'appareil
US20050224692A1 (en) * 2004-02-06 2005-10-13 Olympus Corporation Microscope
DE102004051940A1 (de) * 2004-10-25 2006-04-27 Leica Microsystems Cms Gmbh Beleuchtungseinrichtung in einem Mikroskop

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10233549B4 (de) * 2002-07-23 2021-10-14 Leica Microsystems Cms Gmbh Scanmikroskop mit Manipulationslichtstrahl und Verfahren zur Scanmikroskopie

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6079322A (ja) * 1983-10-07 1985-05-07 Furukawa Electric Co Ltd:The 回転ミラ−付フアイバスコ−プ
WO2003021212A1 (fr) * 2001-08-28 2003-03-13 Baylor College Of Medicine Excitation a lignes multiples par impulsions pour detection par fluorescence non chromatisee
DE10252664A1 (de) * 2002-11-11 2004-06-03 Leica Microsystems Wetzlar Gmbh Mikroskop und Verfahren zur Änderung des Lichtflusses in einem Mikroskop
DE10314125A1 (de) * 2003-03-28 2004-10-21 Carl Zeiss Jena Gmbh Anordnung zur Beleuchtung von Objekten mit Licht unterschiedlicher Wellenlänge
EP1512999A2 (fr) * 2003-08-25 2005-03-09 Olympus Corporation Dispositif optique, appareil d'éclairage utilisant le dispositif, et projecteur utilisant l'appareil
DE20319495U1 (de) * 2003-12-16 2004-03-11 Carl Zeiss Jena Gmbh Beleuchtungseinrichtung für ein Mikroskop
US20050224692A1 (en) * 2004-02-06 2005-10-13 Olympus Corporation Microscope
DE102004051940A1 (de) * 2004-10-25 2006-04-27 Leica Microsystems Cms Gmbh Beleuchtungseinrichtung in einem Mikroskop

Also Published As

Publication number Publication date
DE102006009053A1 (de) 2007-08-30

Similar Documents

Publication Publication Date Title
EP1949082B1 (fr) Dispositif d éclairage multispectral
DE10012462B4 (de) Beleuchtungsvorrichtung für die konfokale Fluoreszenz-Rastermikroskopie
EP2948810B1 (fr) Microscope optique et procédé de microscopie
EP3149532B1 (fr) Microscope à balayage laser à fonctions intégrées
EP2823347B1 (fr) Microscope optique à balayage, à détection spectrale
EP2904442B1 (fr) Microscope à foyer commun avec balayage d'échantillon librement réglable
DE19835072A1 (de) Anordnung zur Beleuchtung und/oder Detektion in einem Mikroskop
DE19906757A1 (de) Optische Anordnung
DE10137155B4 (de) Optische Anordnung und Scanmikroskop
EP1664888B1 (fr) Microscope a balayage avec eclairage evanescent
EP1528421A1 (fr) System d'éclairage à diodes électroluminescentes pour un dispositif d'observation, en particulier pour un stéréomicroscope ou un stéréomicroscope chirurgical
EP1164406A2 (fr) Méthode et appareil pour illuminer un objet
DE102012017920B4 (de) Optikanordnung und Lichtmikroskop
WO2005024482A1 (fr) Source de lumiere comprenant plusieurs elements optiques microstructures
DE102004016253B4 (de) Rastermikroskop und Verfahren zur rastermikroskopischen Untersuchung einer Probe
DE102006056429B3 (de) Lasermikroskop mit räumlich trennendem Strahlteiler
WO2012034852A1 (fr) Système d'imagerie optique pour l'imagerie multispectrale
DE20216583U1 (de) Mikroskop und Durchflusszytometer
DE102016102286A1 (de) Vorrichtung und Verfahren zur Multispot-Scanning-Mikroskopie
DE10115488A1 (de) Verfahren und Vorrichtung zur Beleuchtung eines Objekts
WO2016173662A1 (fr) Appareil de mesure d'émission de lumière et procédé pour la mesure d'une d'émission de lumière
EP1505424A1 (fr) Microscope à balayage avec un moyen de couplage optique pour lumière externe
EP1315012B1 (fr) Microscope à balayage, méthode de microscopie à balayage et filtre passe-bande
DE102020108117B4 (de) Mikroskop und Verfahren zum Betreiben eines Mikroskops
WO2007098821A1 (fr) Dispositif d'éclairage multispectral

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 07711327

Country of ref document: EP

Kind code of ref document: A1