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WO2007076974A3 - Method for the production of a chemically modified protein - Google Patents

Method for the production of a chemically modified protein Download PDF

Info

Publication number
WO2007076974A3
WO2007076974A3 PCT/EP2006/012434 EP2006012434W WO2007076974A3 WO 2007076974 A3 WO2007076974 A3 WO 2007076974A3 EP 2006012434 W EP2006012434 W EP 2006012434W WO 2007076974 A3 WO2007076974 A3 WO 2007076974A3
Authority
WO
WIPO (PCT)
Prior art keywords
chemically modified
modified
production
modified protein
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2006/012434
Other languages
German (de)
French (fr)
Other versions
WO2007076974A2 (en
Inventor
Henning Mootz
Thomas Kurpiers
Christina Ludwig
Steffen Brenzel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philipps Universitaet Marburg
Original Assignee
Philipps Universitaet Marburg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philipps Universitaet Marburg filed Critical Philipps Universitaet Marburg
Publication of WO2007076974A2 publication Critical patent/WO2007076974A2/en
Publication of WO2007076974A3 publication Critical patent/WO2007076974A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1075—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of amino acids or peptide residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Disclosed is a method for producing a chemically modified protein, in which an auxiliary protein that carries a reactive, functional group and is composed of an intein fragment and an extein sequence is modified with a group to be introduced into the target protein in a first step, and a target protein that is fused with the intein fragment to be modified and with a complementary intein fragment is reacted in a second step such that the modified extein sequence is combined with the target protein.
PCT/EP2006/012434 2005-12-23 2006-12-22 Method for the production of a chemically modified protein Ceased WO2007076974A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102005061934.7 2005-12-23
DE200510061934 DE102005061934A1 (en) 2005-12-23 2005-12-23 Method for preparing chemically modified proteins, useful as pharmaceuticals and diagnostic reagents, by protein splicing between two recombinant proteins each comprising an intein and an extein

Publications (2)

Publication Number Publication Date
WO2007076974A2 WO2007076974A2 (en) 2007-07-12
WO2007076974A3 true WO2007076974A3 (en) 2007-11-15

Family

ID=38108818

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2006/012434 Ceased WO2007076974A2 (en) 2005-12-23 2006-12-22 Method for the production of a chemically modified protein

Country Status (2)

Country Link
DE (1) DE102005061934A1 (en)
WO (1) WO2007076974A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2777714A1 (en) * 2013-03-15 2014-09-17 NBE-Therapeutics LLC Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme
US10188745B2 (en) 2014-12-23 2019-01-29 Nbe-Therapeutics Ag Binding protein drug conjugates comprising anthracycline derivatives

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BASHTOVYY DENYS ET AL: "Constrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer", PROTEIN SCIENCE, vol. 10, no. 5, May 2001 (2001-05-01), pages 979 - 987, XP002450475, ISSN: 0961-8368 *
ERNST L A ET AL: "Cyanine Dye Labeling Reagents for Sulfhydryl Groups", CYTOMETRY, ALAN LISS, NEW YORK, US, vol. 10, no. 1, 1989, pages 3 - 10, XP002249385, ISSN: 0196-4763 *
GIRIAT IZABELA ET AL: "Protein semi-synthesis in living cells.", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 125, no. 24, 18 June 2003 (2003-06-18), pages 7180 - 7181, XP002450472, ISSN: 0002-7863 *
GOODY ROGER S ET AL: "Combining chemical and biological techniques to produce modified proteins.", CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY 3 MAY 2002, vol. 3, no. 5, 3 May 2002 (2002-05-03), pages 399 - 403, XP002450476, ISSN: 1439-4227 *
HAHN M E ET AL: "Manipulating proteins with chemistry: a cross-section of chemical biology", TRENDS IN BIOCHEMICAL SCIENCES, ELSEVIER, HAYWARDS, GB, vol. 30, no. 1, January 2005 (2005-01-01), pages 26 - 34, XP004710938, ISSN: 0968-0004 *
STRATIKOS EFSTRATIOS ET AL: "Mapping the serpin-proteinase complex using single cysteine variants of alpha1-proteinase inhibitor Pittsburgh", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 25, 19 June 1998 (1998-06-19), pages 15582 - 15589, XP002450474, ISSN: 0021-9258 *
ZUGER SARA ET AL: "Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies", NATURE BIOTECHNOLOGY, vol. 23, no. 6, June 2005 (2005-06-01), pages 736 - 740, XP002450473, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2007076974A2 (en) 2007-07-12
DE102005061934A1 (en) 2007-06-28

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122 Ep: pct application non-entry in european phase

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