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WO2006138366A3 - Protein markers - Google Patents

Protein markers Download PDF

Info

Publication number
WO2006138366A3
WO2006138366A3 PCT/US2006/023153 US2006023153W WO2006138366A3 WO 2006138366 A3 WO2006138366 A3 WO 2006138366A3 US 2006023153 W US2006023153 W US 2006023153W WO 2006138366 A3 WO2006138366 A3 WO 2006138366A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
protein markers
dyes
dye
marker
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/023153
Other languages
French (fr)
Other versions
WO2006138366A2 (en
Inventor
Dong Ma
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New England Biolabs Inc
Original Assignee
New England Biolabs Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc
Publication of WO2006138366A2 publication Critical patent/WO2006138366A2/en
Publication of WO2006138366A3 publication Critical patent/WO2006138366A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/006—Biological staining of tissues in vivo, e.g. methylene blue or toluidine blue O administered in the buccal area to detect epithelial cancer cells, dyes used for delineating tissues during surgery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
    • C07K1/1077—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/13—Labelling of peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Optics & Photonics (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Cosmetics (AREA)
  • Catching Or Destruction (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A protein marker and a ladder that contains a series of different protein markers are described. Each protein marker is a product of a protein covalently bound to: two or more dyes; one or more dyes and a non-dye protein-binding molecule; or two or more non-dye protein-binding molecules; having a predetermined molecular weight, pi and/or color.
PCT/US2006/023153 2005-06-16 2006-06-15 Protein markers Ceased WO2006138366A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69129905P 2005-06-16 2005-06-16
US60/691,299 2005-06-16

Publications (2)

Publication Number Publication Date
WO2006138366A2 WO2006138366A2 (en) 2006-12-28
WO2006138366A3 true WO2006138366A3 (en) 2007-06-07

Family

ID=36973018

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/023153 Ceased WO2006138366A2 (en) 2005-06-16 2006-06-15 Protein markers

Country Status (1)

Country Link
WO (1) WO2006138366A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9409942B2 (en) 1997-01-08 2016-08-09 Life Technologies Corporation Methods for production of proteins

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009544974A (en) 2006-07-21 2009-12-17 ライフ テクノロジーズ コーポレーション Marked protein molecular weight standard for sharp degradation
GB2512664B (en) * 2013-04-05 2017-09-13 Daniel Brownleader Michael Sample loading buffer for visualisation of target molecules in polyacrylamide gel electrophoresis
WO2015025054A1 (en) 2013-08-22 2015-02-26 Medizinische Universität Wien Dye-specific antibodies for prestained molecular weight markers and methods producing the same
US20170269092A1 (en) 2014-12-02 2017-09-21 Cemm - Forschungszentrum Fuer Molekulare Medizin Gmbh Anti-mutant calreticulin antibodies and their use in the diagnosis and therapy of myeloid malignancies

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070967A2 (en) * 2002-02-20 2003-08-28 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070967A2 (en) * 2002-02-20 2003-08-28 Invitrogen Corporation Highly homogeneous molecular markers for electrophoresis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COMPTON MARK M; LAPP STACEY A; PEDEMONTE RON: "Generation of multicolored, prestained molecular weight markers for gel electrophoresis", ELECTROPHORESIS, vol. 23, no. 19, September 2002 (2002-09-01), pages 3262 - 3265, XP002403202 *
COTTON ET AL: "GENERATION OF A DUAL-LABELED FLUORESCENCE BIOSENSOR FOR CRK-II PHOSPHORYLATION USING SOLID-PHASE EXPRESSED PROTEIN LIGATION", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 7, no. 4, April 2000 (2000-04-01), pages 253 - 261, XP000989902, ISSN: 1074-5521 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9409942B2 (en) 1997-01-08 2016-08-09 Life Technologies Corporation Methods for production of proteins

Also Published As

Publication number Publication date
WO2006138366A2 (en) 2006-12-28

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