[go: up one dir, main page]

WO2006081583A3 - Methods and compositions for improving fidelity in a nucleic acid synthesis reaction - Google Patents

Methods and compositions for improving fidelity in a nucleic acid synthesis reaction Download PDF

Info

Publication number
WO2006081583A3
WO2006081583A3 PCT/US2006/004638 US2006004638W WO2006081583A3 WO 2006081583 A3 WO2006081583 A3 WO 2006081583A3 US 2006004638 W US2006004638 W US 2006004638W WO 2006081583 A3 WO2006081583 A3 WO 2006081583A3
Authority
WO
WIPO (PCT)
Prior art keywords
compositions
synthesis reaction
methods
nucleic acid
acid synthesis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2006/004638
Other languages
French (fr)
Other versions
WO2006081583A2 (en
Inventor
Philip Richard Buzby
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helicos BioSciences Corp
Original Assignee
Helicos BioSciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Helicos BioSciences Corp filed Critical Helicos BioSciences Corp
Priority to EP06734692A priority Critical patent/EP1848829B1/en
Priority to CA002599477A priority patent/CA2599477A1/en
Priority to AT06734692T priority patent/ATE548466T1/en
Publication of WO2006081583A2 publication Critical patent/WO2006081583A2/en
Publication of WO2006081583A3 publication Critical patent/WO2006081583A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3' hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide.
PCT/US2006/004638 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction Ceased WO2006081583A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP06734692A EP1848829B1 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA002599477A CA2599477A1 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
AT06734692T ATE548466T1 (en) 2005-01-28 2006-01-30 METHODS AND COMPOSITIONS FOR IMPROVING THE ACCURACY OF A NUCLEIC ACID SYNTHESIS REACTION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/046,448 2005-01-28
US11/046,448 US7482120B2 (en) 2005-01-28 2005-01-28 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction

Publications (2)

Publication Number Publication Date
WO2006081583A2 WO2006081583A2 (en) 2006-08-03
WO2006081583A3 true WO2006081583A3 (en) 2006-10-19

Family

ID=36582013

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/004638 Ceased WO2006081583A2 (en) 2005-01-28 2006-01-30 Methods and compositions for improving fidelity in a nucleic acid synthesis reaction

Country Status (5)

Country Link
US (1) US7482120B2 (en)
EP (1) EP1848829B1 (en)
AT (1) ATE548466T1 (en)
CA (1) CA2599477A1 (en)
WO (1) WO2006081583A2 (en)

Families Citing this family (141)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE273381T1 (en) * 1997-02-12 2004-08-15 Eugene Y Chan METHOD FOR ANALYZING POLYMERS
JP2004513619A (en) * 2000-07-07 2004-05-13 ヴィジゲン　バイオテクノロジーズ　インコーポレイテッド Real-time sequencing
WO2002044425A2 (en) * 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7622296B2 (en) 2004-05-28 2009-11-24 Wafergen, Inc. Apparatus and method for multiplex analysis
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
WO2009102470A2 (en) 2008-02-12 2009-08-20 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US20090226906A1 (en) * 2008-03-05 2009-09-10 Helicos Biosciences Corporation Methods and compositions for reducing nucleotide impurities
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
CN102625838B (en) 2009-08-14 2016-01-20 阿霹震中科技公司 For generating sample that rRNA eliminates or for from the method for sample separation rRNA, composition and test kit
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
CA2798635A1 (en) 2010-05-06 2011-11-10 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
WO2012012703A2 (en) 2010-07-23 2012-01-26 Esoterix Genetic Laboratories, Llc Identification of differentially represented fetal or maternal genomic regions and uses thereof
US20120070830A1 (en) 2010-09-16 2012-03-22 lbis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
SG191409A1 (en) 2010-12-27 2013-08-30 Ibis Biosciences Inc Nucleic acid sample preparation methods and compositions
US9670538B2 (en) 2011-08-05 2017-06-06 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US20150329855A1 (en) 2011-12-22 2015-11-19 Ibis Biosciences, Inc. Amplification primers and methods
EP3269820A1 (en) 2011-12-22 2018-01-17 Ibis Biosciences, Inc. Kit for the amplification of a sequence from a ribonucleic acid
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
US9822417B2 (en) 2012-01-09 2017-11-21 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
WO2013106737A1 (en) 2012-01-13 2013-07-18 Data2Bio Genotyping by next-generation sequencing
US9732387B2 (en) 2012-04-03 2017-08-15 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and Crohn's disease
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
EP2844767A4 (en) 2012-05-02 2015-11-18 Ibis Biosciences Inc Nucleic acid sequencing systems and methods
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
WO2014062835A1 (en) 2012-10-16 2014-04-24 Abbott Molecular Inc. Methods and apparatus to sequence a nucleic acid
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014152937A1 (en) 2013-03-14 2014-09-25 Ibis Biosciences, Inc. Nucleic acid control panels
WO2014160117A1 (en) 2013-03-14 2014-10-02 Abbott Molecular Inc. Multiplex methylation-specific amplification systems and methods
CN105358709B (en) 2013-03-15 2018-12-07 雅培分子公司 System and method for detecting genome copy numbers variation
ES2716094T3 (en) 2013-03-15 2019-06-10 Ibis Biosciences Inc Methods to analyze contamination in DNA sequencing
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
US20150051088A1 (en) 2013-08-19 2015-02-19 Abbott Molecular Inc. Next-generation sequencing libraries
EP3057985A4 (en) 2013-10-18 2017-05-31 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
EP3083700B1 (en) 2013-12-17 2023-10-11 The Brigham and Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
IL286474B2 (en) 2014-06-23 2023-11-01 Massachusetts Gen Hospital Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
CN114214314A (en) 2014-06-24 2022-03-22 生物辐射实验室股份有限公司 Digital PCR barcoding
CN106715718B (en) 2014-07-24 2021-07-06 雅培分子公司 Compositions and methods for detection and analysis of Mycobacterium tuberculosis
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
WO2016134342A1 (en) 2015-02-20 2016-08-25 Wafergen, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
AU2016250281A1 (en) 2015-04-15 2017-10-26 The General Hospital Corporation LNA-based mutant enrichment next-generation sequencing assays
EP3322805B1 (en) 2015-07-14 2021-10-20 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides or magnesium-titanium oxides
US10526664B2 (en) 2015-07-14 2020-01-07 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
EP3325642B1 (en) 2015-07-21 2020-05-27 Omniome, Inc. Nucleic acid sequencing method
EP3347467B1 (en) 2015-09-11 2021-06-23 The General Hospital Corporation Full interrogation of nuclease dsbs and sequencing (find-seq)
JP2018529353A (en) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション Comprehensive in vitro reporting of cleavage events by sequencing (CIRCLE-seq)
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
AU2017218431B2 (en) 2016-02-08 2022-08-04 RGENE, Inc. Multiple ligase compositions, systems, and methods
WO2017160788A2 (en) 2016-03-14 2017-09-21 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
EP3445870B1 (en) 2016-04-22 2022-09-07 Pacific Biosciences of California, Inc. Nucleic acid sequencing method and system employing enhanced detection of nucleotide-specific ternary complex formation
US10294514B2 (en) 2016-04-29 2019-05-21 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
CN109154024B (en) 2016-04-29 2022-03-04 欧姆尼欧美公司 Nucleic acid sequence determination method
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
EP3485032B1 (en) 2016-07-12 2021-02-17 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
CN109070044B (en) 2016-07-21 2021-07-30 宝生物工程（美国)有限公司 Multi-Z Plane Imaging and Assignment Using Multiwell Devices
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP4039824A1 (en) 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
CN110139932B (en) 2016-12-19 2024-05-17 生物辐射实验室股份有限公司 Drop-on labeled DNA with maintained adjacency
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
WO2018132389A1 (en) 2017-01-10 2018-07-19 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
CA3050695C (en) 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
AU2017394644B2 (en) 2017-01-20 2020-02-06 Pacific Biosciences Of California, Inc. Allele-specific capture of nucleic acids
SG11201906569XA (en) 2017-01-20 2019-08-27 Omniome Inc Genotyping by polymerase binding
EP3354746B1 (en) 2017-01-30 2019-05-29 Gregor Mendel Institute of Molecular Plant Biology GmbH Novel spike-in oligonucleotides for normalization of sequence data
WO2018152162A1 (en) 2017-02-15 2018-08-23 Omniome, Inc. Distinguishing sequences by detecting polymerase dissociation
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
AU2018279728B2 (en) 2017-06-08 2023-07-20 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
JP7390285B2 (en) 2017-06-26 2023-12-01 ウニヴェルズィテート・フューア・ボーデンクルトゥーア・ウィーン Novel biomarker for detecting senescent cells
CA3075932A1 (en) 2017-09-20 2019-03-28 Guardant Health, Inc. Methods and systems for differentiating somatic and germline variants
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
CN118186055A (en) 2017-11-02 2024-06-14 生物辐射实验室股份有限公司 Transposase-based genomic analysis
EP4324962A3 (en) 2018-01-31 2024-05-08 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
EP3784800B1 (en) 2018-04-26 2023-05-03 Pacific Biosciences of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
WO2019213619A1 (en) 2018-05-04 2019-11-07 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
EP3802874A1 (en) 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
US20190385700A1 (en) 2018-06-04 2019-12-19 Guardant Health, Inc. METHODS AND SYSTEMS FOR DETERMINING The CELLULAR ORIGIN OF CELL-FREE NUCLEIC ACIDS
CN120193081A (en) 2018-07-23 2025-06-24 夸登特健康公司 Methods and systems for adjusting tumor mutation burden by tumor fraction and coverage
EP3827097A1 (en) 2018-07-24 2021-06-02 Omniome, Inc. Serial formation of ternary complex species
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
JP2021536232A (en) 2018-08-30 2021-12-27 ガーダント ヘルス， インコーポレイテッド Methods and systems for detecting contamination between samples
AU2019328344B2 (en) 2018-08-31 2025-06-12 Guardant Health, Inc. Microsatellite instability detection in cell-free DNA
WO2020096691A2 (en) 2018-09-04 2020-05-14 Guardant Health, Inc. Methods and systems for detecting allelic imbalance in cell-free nucleic acid samples
EP3814533B1 (en) 2018-09-20 2021-08-11 TAmiRNA GmbH Micro-rna signatures for the prediction of liver dysfunction
CN113227393B (en) 2018-10-31 2025-02-11 夸登特健康公司 Methods, compositions and systems for calibrating epigenetic partitioning assays
US12139764B2 (en) 2018-11-14 2024-11-12 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating breast cancer
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
CN113166805B (en) 2018-12-04 2024-07-16 加利福尼亚太平洋生物科学股份有限公司 Mixed-phase fluids for nucleic acid sequencing and other analytical assays
CN113454218A (en) 2018-12-20 2021-09-28 夸登特健康公司 Methods, compositions, and systems for improved recovery of nucleic acid molecules
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
EP3918089B1 (en) 2019-01-31 2025-01-15 Guardant Health, Inc. Method for isolating and sequencing cell-free dna
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
EP3976822A1 (en) 2019-05-31 2022-04-06 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
AU2020325026A1 (en) 2019-08-05 2022-03-17 Mission Bio, Inc. Method and apparatus for single-cell analysis for determining a cell trajectory
EP3999651A4 (en) 2019-08-26 2023-07-26 PACT Pharma, Inc. METHODS FOR PERFORMING GUIDED SEQUENCING ON PRIMARY HUMAN T LYMPHOCYTES
EP4045683B1 (en) 2019-10-18 2025-02-19 Pacific Biosciences of California, Inc. Methods for capping nucleic acids
ES2938896T3 (en) 2019-10-21 2023-04-17 Univ Freiburg Albert Ludwigs A truly unbiased in vitro assay to profile the off-target activity of one or more target-specific programmable nucleases in cells (ABNOBA-SEQ)
ES3031713T3 (en) 2019-11-26 2025-07-10 Guardant Health Inc Methods, compositions and systems for improving the binding of methylated polynucleotides
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021188889A1 (en) 2020-03-20 2021-09-23 Mission Bio, Inc. Single cell workflow for whole genome amplification
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021222828A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US20230183798A1 (en) 2020-05-05 2023-06-15 Pacific Biosciences Of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
US12241126B2 (en) 2020-05-14 2025-03-04 Guardant Health, Inc. Homologous recombination repair deficiency detection
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
EP4179111B1 (en) 2020-07-10 2024-04-03 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US12116637B2 (en) 2020-07-24 2024-10-15 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
EP4585697A3 (en) 2020-07-30 2025-08-06 Guardant Health, Inc. Methods for isolating cell-free dna
JP2023540221A (en) 2020-08-25 2023-09-22 ガーダント ヘルス， インコーポレイテッド Methods and systems for predicting variant origin
US20220162670A1 (en) 2020-09-30 2022-05-26 Guardant Health, Inc. Methods and systems to improve the signal to noise ratio of dna methylation partitioning assays
CA3195797A1 (en) 2020-10-23 2022-04-28 Andrew Kennedy Compositions and methods for analyzing dna using partitioning and base conversion
WO2022140629A1 (en) 2020-12-23 2022-06-30 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
EP4302301A1 (en) 2021-03-05 2024-01-10 Guardant Health, Inc. Methods and related aspects for analyzing molecular response
EP4305200A1 (en) 2021-03-09 2024-01-17 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
EP4523213A1 (en) 2022-05-09 2025-03-19 Guardant Health, Inc. Detecting degradation based on strand bias
KR20250110272A (en) 2022-11-15 2025-07-18 가던트 헬쓰, 인크. Methods for predicting non-small cell lung cancer (NSCLC) patient drug response or time to death or cancer progression using signals from both baseline CTDNA levels and longitudinal changes in CTDNA levels over time from circulating tumor DNA (CTDNA)
CN120569782A (en) 2022-12-21 2025-08-29 夸登特健康公司 Detection of homologous recombination defects based on methylation status of cell-free nucleic acid molecules
WO2024216163A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using predominantly unlabeled nucleotides
WO2024216159A1 (en) 2023-04-13 2024-10-17 Esbiolab Llc Methods and compositions for nucleic acid sequencing using labeled nucleotides
WO2024233502A1 (en) 2023-05-05 2024-11-14 Guardant Health, Inc. Cell-free dna blood-based test for cancer screening
US20240420800A1 (en) 2023-06-15 2024-12-19 Guardant Health, Inc. METHOD FOR HRD DETECTION IN TARGETED cfDNA SAMPLES USING DE NOVO MUTATIONAL SIGNATURES
WO2025072467A1 (en) 2023-09-29 2025-04-03 Guardant Health, Inc. Genotyping cyp2d6
US20250285708A1 (en) 2023-10-27 2025-09-11 Guardant Health, Inc. Monitoring molecular response by allelic imbalance
WO2025235623A1 (en) 2024-05-08 2025-11-13 Esbiolab, Llc Methods and systems for nucleic acid sequencing using an error-buffered coding scheme

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0481065A1 (en) * 1990-05-07 1992-04-22 Stanford Res Inst Int PROCESS OF HYBRIDIZATION AND AMPLIFICATION OF NUCLEIC ACID.
US5976842A (en) * 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
WO2002044425A2 (en) * 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2004074503A2 (en) * 2003-02-21 2004-09-02 Hoser Mark J Nucleic acid sequencing methods, kits and reagents

Family Cites Families (463)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3996345A (en) 1974-08-12 1976-12-07 Syva Company Fluorescence quenching with immunological pairs in immunoassays
JPS5941169B2 (en) 1975-12-25 1984-10-05 シチズン時計株式会社 Elastomer
US4153855A (en) 1977-12-16 1979-05-08 The United States Of America As Represented By The Secretary Of The Army Method of making a plate having a pattern of microchannels
US4351760A (en) 1979-09-07 1982-09-28 Syva Company Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
US4344064A (en) 1979-12-06 1982-08-10 Western Electric Co., Inc. Article carrying a distinctive mark
US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5260433A (en) 1982-06-23 1993-11-09 Enzo Diagnostics, Inc. Saccharide specific binding system labeled nucleotides
US4707237A (en) 1982-09-09 1987-11-17 Ciba Corning Diagnostics Corp. System for identification of cells by electrophoresis
US5198540A (en) * 1982-10-28 1993-03-30 Hubert Koster Process for the preparation of oligonucleotides in solution
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
DE3329892A1 (en) * 1983-08-18 1985-03-07 Köster, Hubert, Prof. Dr., 2000 Hamburg METHOD FOR PRODUCING OLIGONUCLEOTIDES
USRE34069E (en) 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US4581624A (en) 1984-03-01 1986-04-08 Allied Corporation Microminiature semiconductor valve
US5360523A (en) 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US4729947A (en) 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US5258506A (en) 1984-10-16 1993-11-02 Chiron Corporation Photolabile reagents for incorporation into oligonucleotide chains
US6060237A (en) 1985-02-26 2000-05-09 Biostar, Inc. Devices and methods for optical detection of nucleic acid hybridization
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4865968A (en) 1985-04-01 1989-09-12 The Salk Institute For Biological Studies DNA sequencing
US4739044A (en) * 1985-06-13 1988-04-19 Amgen Method for derivitization of polynucleotides
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
US4757141A (en) 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US5242797A (en) 1986-03-21 1993-09-07 Myron J. Block Nucleic acid assay method
US4811218A (en) * 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
CA1340806C (en) 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US4994372A (en) * 1987-01-14 1991-02-19 President And Fellows Of Harvard College DNA sequencing
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US4994368A (en) * 1987-07-23 1991-02-19 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US4942124A (en) 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
US4793705A (en) 1987-10-07 1988-12-27 The United States Of America As Represented By The United States Department Of Energy Single molecule tracking
US4962037A (en) 1987-10-07 1990-10-09 United States Of America Method for rapid base sequencing in DNA and RNA
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
CH679555A5 (en) * 1989-04-11 1992-03-13 Westonbridge Int Ltd
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US4979824A (en) 1989-05-26 1990-12-25 Board Of Trustees Of The Leland Stanford Junior University High sensitivity fluorescent single particle and single molecule detection apparatus and method
US6346413B1 (en) * 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5800992A (en) * 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH04501449A (en) 1989-06-14 1992-03-12 ウエストンブリッジ　インターナショナル　リミティド micro pump
US5112736A (en) 1989-06-14 1992-05-12 University Of Utah Dna sequencing using fluorescence background electroblotting membrane
US5108892A (en) * 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
US5096554A (en) * 1989-08-07 1992-03-17 Applied Biosystems, Inc. Nucleic acid fractionation by counter-migration capillary electrophoresis
FR2650840B1 (en) 1989-08-11 1991-11-29 Bertin & Cie RAPID DETECTION AND / OR IDENTIFICATION OF A SINGLE BASED ON A NUCLEIC ACID SEQUENCE, AND ITS APPLICATIONS
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2028261C (en) 1989-10-28 1995-01-17 Won Suck Yang Non-invasive method and apparatus for measuring blood glucose concentration
US5171132A (en) 1989-12-27 1992-12-15 Seiko Epson Corporation Two-valve thin plate micropump
US5091652A (en) * 1990-01-12 1992-02-25 The Regents Of The University Of California Laser excited confocal microscope fluorescence scanner and method
WO1991011533A1 (en) * 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
DE4006152A1 (en) 1990-02-27 1991-08-29 Fraunhofer Ges Forschung MICROMINIATURIZED PUMP
US5096388A (en) * 1990-03-22 1992-03-17 The Charles Stark Draper Laboratory, Inc. Microfabricated pump
SE470347B (en) 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Microstructure for fluid flow systems and process for manufacturing such a system
US5259737A (en) 1990-07-02 1993-11-09 Seiko Epson Corporation Micropump with valve structure
DE69132905T2 (en) 1990-12-06 2002-08-01 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Detection of nucleic acid sequences
DE69132531T2 (en) 1990-12-06 2001-09-13 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Compounds and their use in a binary synthesis strategy
US5529679A (en) 1992-02-28 1996-06-25 Hitachi, Ltd. DNA detector and DNA detection method
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5762876A (en) 1991-03-05 1998-06-09 Molecular Tool, Inc. Automatic genotype determination
US5888819A (en) * 1991-03-05 1999-03-30 Molecular Tool, Inc. Method for determining nucleotide identity through primer extension
US5167784A (en) 1991-09-04 1992-12-01 Xerox Corporation Sequencing large nucleic acid fragments
WO1993005183A1 (en) 1991-09-09 1993-03-18 Baylor College Of Medicine Method and device for rapid dna or rna sequencing determination by a base addition sequencing scheme
DE4143343C2 (en) 1991-09-11 1994-09-22 Fraunhofer Ges Forschung Microminiaturized, electrostatically operated micromembrane pump
US5265327A (en) 1991-09-13 1993-11-30 Faris Sadeg M Microchannel plate technology
CA2118806A1 (en) 1991-09-18 1993-04-01 William J. Dower Method of synthesizing diverse collections of oligomers
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US5756285A (en) 1991-09-27 1998-05-26 Amersham Life Science, Inc. DNA cycle sequencing
US5674679A (en) 1991-09-27 1997-10-07 Amersham Life Science, Inc. DNA cycle sequencing
US6051380A (en) 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
WO1993013225A1 (en) 1991-12-23 1993-07-08 Chiron Corporation Htlv-1 probes for use in solution phase sandwich hybridization assays
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
US5209834A (en) 1992-03-09 1993-05-11 The United States Of America As Represented By The United States Department Of Energy Ordered transport and identification of particles
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5304487A (en) * 1992-05-01 1994-04-19 Trustees Of The University Of Pennsylvania Fluid handling in mesoscale analytical devices
DE4220077A1 (en) 1992-06-19 1993-12-23 Bosch Gmbh Robert Micro-pump for delivery of gases - uses working chamber warmed by heating element and controlled by silicon wafer valves.
JPH0622798A (en) 1992-07-07 1994-02-01 Hitachi Ltd Method for determining base sequence
US5284568A (en) 1992-07-17 1994-02-08 E. I. Du Pont De Nemours And Company Disposable cartridge for ion selective electrode sensors
US5306403A (en) * 1992-08-24 1994-04-26 Martin Marietta Energy Systems, Inc. Raman-based system for DNA sequencing-mapping and other separations
US5403709A (en) * 1992-10-06 1995-04-04 Hybridon, Inc. Method for sequencing synthetic oligonucleotides containing non-phosphodiester internucleotide linkages
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
AU671820B2 (en) 1993-02-10 1996-09-12 Promega Corporation Non-radioactive DNA sequencing
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
AU7212494A (en) 1993-06-25 1995-01-17 Affymax Technologies N.V. Hybridization and sequencing of nucleic acids
US5837832A (en) 1993-06-25 1998-11-17 Affymetrix, Inc. Arrays of nucleic acid probes on biological chips
US5547859A (en) 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
DE4326466A1 (en) 1993-08-06 1995-02-09 Boehringer Mannheim Gmbh Infrared dye-labeled nucleotides and their use in nucleic acid detection
US6376178B1 (en) 1993-09-03 2002-04-23 Duke University Method of nucleic acid sequencing
US5659171A (en) 1993-09-22 1997-08-19 Northrop Grumman Corporation Micro-miniature diaphragm pump for the low pressure pumping of gases
US6401267B1 (en) 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US5470710A (en) * 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US6309601B1 (en) 1993-11-01 2001-10-30 Nanogen, Inc. Scanning optical detection system
NZ276860A (en) 1993-11-02 1997-09-22 Affymax Tech Nv Apparatus and its use for synthesising diverse molecular products on substrates
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
CH689836A5 (en) 1994-01-14 1999-12-15 Westonbridge Int Ltd Micropump.
US6028190A (en) * 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
WO1995021271A1 (en) 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
US5631734A (en) 1994-02-10 1997-05-20 Affymetrix, Inc. Method and apparatus for detection of fluorescently labeled materials
US5578832A (en) 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
JP3448090B2 (en) 1994-02-16 2003-09-16 浜松ホトニクス株式会社 Energy transfer detection method and apparatus
AU1854895A (en) 1994-03-08 1995-09-25 Amersham International Plc Modifying nucleotide analogues
US20020168642A1 (en) 1994-06-06 2002-11-14 Andrzej Drukier Sequencing duplex DNA by mass spectroscopy
US6287850B1 (en) 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US6379897B1 (en) 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US5834189A (en) 1994-07-08 1998-11-10 Visible Genetics Inc. Method for evaluation of polymorphic genetic sequences, and the use thereof in identification of HLA types
US5853979A (en) 1995-06-30 1998-12-29 Visible Genetics Inc. Method and system for DNA sequence determination and mutation detection with reference to a standard
US6001229A (en) 1994-08-01 1999-12-14 Lockheed Martin Energy Systems, Inc. Apparatus and method for performing microfluidic manipulations for chemical analysis
US5763594A (en) 1994-09-02 1998-06-09 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
US5808045A (en) 1994-09-02 1998-09-15 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
US6214987B1 (en) 1994-09-02 2001-04-10 Andrew C. Hiatt Compositions for enzyme catalyzed template-independent formation of phosphodiester bonds using protected nucleotides
US5872244A (en) * 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds
DE4433894A1 (en) 1994-09-22 1996-03-28 Fraunhofer Ges Forschung Method and device for controlling a micropump
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
DE69531430T2 (en) 1994-10-07 2004-07-01 Bayer Corp. relief valve
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US6406848B1 (en) 1997-05-23 2002-06-18 Lynx Therapeutics, Inc. Planar arrays of microparticle-bound polynucleotides
US6654505B2 (en) 1994-10-13 2003-11-25 Lynx Therapeutics, Inc. System and apparatus for sequential processing of analytes
US5604097A (en) 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5707506A (en) * 1994-10-28 1998-01-13 Battelle Memorial Institute Channel plate for DNA sequencing
US5514256A (en) 1994-10-28 1996-05-07 Battelle Memorial Institute Apparatus for improved DNA sequencing
FR2726286B1 (en) 1994-10-28 1997-01-17 Genset Sa SOLID PHASE NUCLEIC ACID AMPLIFICATION PROCESS AND REAGENT KIT USEFUL FOR CARRYING OUT SAID PROCESS
EP0791238B1 (en) 1994-11-10 2004-09-22 Orchid BioSciences, Inc. Liquid distribution system
US5603351A (en) 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5556790A (en) 1994-12-05 1996-09-17 Pettit; John W. Method for Automated DNA sequencing
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
US5786142A (en) * 1995-05-30 1998-07-28 Visible Genetics Inc. Electrophoresis and fluorescence detection method
SE9500342D0 (en) 1995-01-31 1995-01-31 Marek Kwiatkowski Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation
SE9500589A0 (en) 1995-02-17 1996-08-18 Rigler Rudolf Nucleic acid molecule detection on solid substrates in single-molecule DNA sequencing
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US5599695A (en) * 1995-02-27 1997-02-04 Affymetrix, Inc. Printing molecular library arrays using deprotection agents solely in the vapor phase
US5876187A (en) * 1995-03-09 1999-03-02 University Of Washington Micropumps with fixed valves
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) * 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5994058A (en) 1995-03-20 1999-11-30 Genome International Corporation Method for contiguous genome sequencing
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5675155A (en) 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
WO1996038568A1 (en) * 1995-05-31 1996-12-05 Amersham Life Science, Inc. Thermostable dna polymerases
US6077664A (en) 1995-06-07 2000-06-20 Promega Corporation Thermophilic DNA polymerases from Thermotoga neapolitana
US5861287A (en) * 1995-06-23 1999-01-19 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
CN1106937C (en) 1995-06-26 2003-04-30 美国3M公司 Multilayer polymer film with additional coatings or layers
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5916747A (en) 1995-06-30 1999-06-29 Visible Genetics Inc. Method and apparatus for alignment of signals for use in DNA based-calling
US5981186A (en) 1995-06-30 1999-11-09 Visible Genetics, Inc. Method and apparatus for DNA-sequencing using reduced number of sequencing mixtures
US5807679A (en) 1995-07-07 1998-09-15 Myriad Genetics, Inc. Island hopping--a method to sequence rapidly very large fragments of DNA
US5968740A (en) 1995-07-24 1999-10-19 Affymetrix, Inc. Method of Identifying a Base in a Nucleic Acid
DE19527155C2 (en) 1995-07-25 1998-12-10 Deutsches Krebsforsch Method of sequencing by oligomer hybridization
WO1997009451A1 (en) 1995-09-08 1997-03-13 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US5733729A (en) * 1995-09-14 1998-03-31 Affymetrix, Inc. Computer-aided probability base calling for arrays of nucleic acid probes on chips
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5843655A (en) 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US6132580A (en) 1995-09-28 2000-10-17 The Regents Of The University Of California Miniature reaction chamber and devices incorporating same
US6165765A (en) 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
SE9504099D0 (en) * 1995-11-16 1995-11-16 Pharmacia Biotech Ab A method of sequencing
US5776767A (en) 1995-12-12 1998-07-07 Visible Genetics Inc. Virtual DNA sequencer
US5705018A (en) * 1995-12-13 1998-01-06 Hartley; Frank T. Micromachined peristaltic pump
US6147205A (en) 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
DE69613856T2 (en) 1995-12-15 2002-04-04 Amersham Pharmacia Biotech Inc., Piscataway THERMOSTABLE DNA POLYMERASE FROM THERMOANAEROBACTER THERMOHYDROSULFURICUS AND DERIVED MUTED ENZYMES WITH REMOVED EXONUCLEASE ACTIVITY
US5945283A (en) 1995-12-18 1999-08-31 Washington University Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer
KR100207410B1 (en) 1995-12-19 1999-07-15 전주범 Manufacturing method of optical path control device
US5789168A (en) 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5830657A (en) 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5795722A (en) 1997-03-18 1998-08-18 Visible Genetics Inc. Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US20010018514A1 (en) 1998-07-31 2001-08-30 Mcgall Glenn H. Nucleic acid labeling compounds
US6312893B1 (en) 1996-01-23 2001-11-06 Qiagen Genomics, Inc. Methods and compositions for determining the sequence of nucleic acid molecules
TW406461B (en) 1996-03-01 2000-09-21 Cooper Ind Inc Enhanced polymer ic weathershed and surge arrester and method of making same
AU2217597A (en) 1996-03-18 1997-10-22 Sequenom, Inc. Dna sequencing by mass spectrometry
US6361937B1 (en) * 1996-03-19 2002-03-26 Affymetrix, Incorporated Computer-aided nucleic acid sequencing
AU2320597A (en) 1996-03-19 1997-10-10 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
SE9601318D0 (en) * 1996-04-04 1996-04-04 Pharmacia Biosensor Ab Method for nucleic acid analysis
AU2804597A (en) 1996-04-15 1997-11-07 University Of Alberta Synthesis of fluorophore-labeled dna
US5867266A (en) 1996-04-17 1999-02-02 Cornell Research Foundation, Inc. Multiple optical channels for chemical analysis
US6432634B1 (en) 1996-04-18 2002-08-13 Visible Genetics Inc. Method, apparatus and kits for sequencing of nucleic acids using multiple dyes
WO1997040104A1 (en) 1996-04-19 1997-10-30 Amersham Pharmacia Biotech Uk Limited Squarate dyes and their use in fluorescent sequencing method
JP3418292B2 (en) 1996-04-24 2003-06-16 株式会社日立製作所 Gene analyzer
AU2747597A (en) 1996-05-01 1997-11-19 Visible Genetics Inc. Method, compositions and kit for detection and identification of microorganisms
US5928906A (en) 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
JP2000512744A (en) * 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド System and method for detecting label material
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
WO1997047761A1 (en) 1996-06-14 1997-12-18 Sarnoff Corporation Method for polynucleotide sequencing
US5771124A (en) 1996-07-02 1998-06-23 Siliscape Compact display system with two stage magnification and immersed beam splitter
EP0854935B1 (en) 1996-07-16 2005-04-13 Periannan Senapathy Method for contiguous genome sequencing
WO1998003684A1 (en) 1996-07-19 1998-01-29 Hybridon, Inc. Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
US5922608A (en) 1996-07-31 1999-07-13 Beckman Instruments, Inc. Macromolecule sequencing packet and method of use
US6136212A (en) 1996-08-12 2000-10-24 The Regents Of The University Of Michigan Polymer-based micromachining for microfluidic devices
AU4236597A (en) 1996-08-27 1998-03-19 Visible Genetics Inc. Apparatus and method for performing sequencing of nucleic acid polymers
US5832165A (en) 1996-08-28 1998-11-03 University Of Utah Research Foundation Composite waveguide for solid phase binding assays
US6221654B1 (en) 1996-09-25 2001-04-24 California Institute Of Technology Method and apparatus for analysis and sorting of polynucleotides based on size
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US6020457A (en) * 1996-09-30 2000-02-01 Dendritech Inc. Disulfide-containing dendritic polymers
US5965446A (en) 1996-10-24 1999-10-12 Hamamatsu Photonics K.K. Method for placing fluorescent single molecules on surface of substrate and method for visualizing structural defect of surface of substrate
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6002471A (en) 1996-11-04 1999-12-14 California Institute Of Technology High resolution scanning raman microscope
CA2270132A1 (en) 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
DE19782097T1 (en) 1996-11-06 1999-10-14 Sequenom Inc Compositions and methods for immobilizing nucleic acids on solid supports
DE69727489T2 (en) 1996-11-06 2004-11-25 Sequenom, Inc., San Diego METHOD OF MASS SPECTROMETRY
US6024925A (en) * 1997-01-23 2000-02-15 Sequenom, Inc. Systems and methods for preparing low volume analyte array elements
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6140053A (en) 1996-11-06 2000-10-31 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
US6225062B1 (en) 1996-11-12 2001-05-01 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
US5971355A (en) 1996-11-27 1999-10-26 Xerox Corporation Microdevice valve structures to fluid control
US5958703A (en) 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
US6017702A (en) * 1996-12-05 2000-01-25 The Perkin-Elmer Corporation Chain-termination type nucleic acid sequencing method including 2'-deoxyuridine-5'-triphosphate
US5876934A (en) * 1996-12-18 1999-03-02 Pharmacia Biotech Inc. DNA sequencing method
DE19653439A1 (en) 1996-12-20 1998-07-02 Svante Dr Paeaebo Methods for the direct, exponential amplification and sequencing of DNA molecules and their application
DE19653494A1 (en) 1996-12-20 1998-06-25 Svante Dr Paeaebo Process for decoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6046005A (en) 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6309824B1 (en) 1997-01-16 2001-10-30 Hyseq, Inc. Methods for analyzing a target nucleic acid using immobilized heterogeneous mixtures of oligonucleotide probes
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
ATE273381T1 (en) * 1997-02-12 2004-08-15 Eugene Y Chan METHOD FOR ANALYZING POLYMERS
US6117973A (en) 1997-02-24 2000-09-12 Georgia Tech Research Corp. PNA monomers with electron donor or acceptor
US5837860A (en) 1997-03-05 1998-11-17 Molecular Tool, Inc. Covalent attachment of nucleic acid molecules onto solid-phases via disulfide bonds
US6117634A (en) * 1997-03-05 2000-09-12 The Reagents Of The University Of Michigan Nucleic acid sequencing and mapping
AU6553498A (en) 1997-03-14 1998-09-29 Hybridon, Inc. Method for sequencing of modified nucleic acids using electrospray ionization-fourier transform mass spectrometry
WO1998041657A1 (en) 1997-03-20 1998-09-24 Affymetrix, Inc. Iterative resequencing
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
AU746892B2 (en) 1997-04-04 2002-05-02 Caliper Life Sciences, Inc. Closed-loop biochemical analyzers
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6235471B1 (en) 1997-04-04 2001-05-22 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US5945284A (en) 1997-05-27 1999-08-31 The Perkin-Elmer Corporation Length determination of nucleic acid repeat sequences by discontinuous primer extension
SE9702008D0 (en) 1997-05-28 1997-05-28 Pharmacia Biotech Ab A method and a system for nucleic acid seouence analysis
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
WO1999001768A1 (en) 1997-07-04 1999-01-14 Nycomed Amersham Plc Peroxidase-catalysed fluorescence
JP4549528B2 (en) 1997-07-28 2010-09-22 ジーイー・ヘルスケア・リミテッド Cyanine dye
CA2294962C (en) 1997-07-28 2012-09-18 Medical Biosystems Ltd. Nucleic acid sequence analysis
US6245506B1 (en) 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
GB9716231D0 (en) * 1997-07-31 1997-10-08 Amersham Int Ltd Base analogues
US5882904A (en) * 1997-08-04 1999-03-16 Amersham Pharmacia Biotech Inc. Thermococcus barossii DNA polymerase mutants
EP1003798B1 (en) 1997-08-12 2005-10-19 Eastman Chemical Company Acrylic modified waterborne sulfonated alkyd dispersions
JP4219088B2 (en) 1997-08-20 2009-02-04 エフシーアイ・アメリカズ・テクノロジー・インコーポレーテッド Receptacle used for high speed modular electrical connectors
US5994085A (en) 1997-08-26 1999-11-30 Cantor; Thomas L. Methods and devices for detecting non-complexed prostate specific antigen
US6087099A (en) 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
AU9476598A (en) 1997-09-11 1999-03-29 Seq, Ltd. Method to make fluorescent nucleotide photoproducts for dna sequencing and analysis
WO1999013109A1 (en) 1997-09-11 1999-03-18 Seq, Ltd. Method for dna sequencing analysis
US6346379B1 (en) 1997-09-11 2002-02-12 F. Hoffman-La Roche Ag Thermostable DNA polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
TW352471B (en) 1997-09-20 1999-02-11 United Microelectronics Corp Method for preventing B-P-Si glass from subsiding
US5836750A (en) 1997-10-09 1998-11-17 Honeywell Inc. Electrostatically actuated mesopump having a plurality of elementary cells
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6511803B1 (en) * 1997-10-10 2003-01-28 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US6049380A (en) 1997-11-12 2000-04-11 Regents Of The University Of California Single molecule identification using selected fluorescence characteristics
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US6268131B1 (en) 1997-12-15 2001-07-31 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids
US6262838B1 (en) 1998-03-20 2001-07-17 Genetic Microsystems Inc Focusing in microscope systems
US6407858B1 (en) 1998-05-14 2002-06-18 Genetic Microsystems, Inc Focusing of microscopes and reading of microarrays
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
JP4317953B2 (en) 1998-01-22 2009-08-19 独立行政法人理化学研究所 DNA sequence determination method
WO1999037810A1 (en) 1998-01-23 1999-07-29 Amersham Pharmacia Biotech, Inc. A method, reagent solution and kits for dna sequencing
WO1999039001A2 (en) 1998-02-02 1999-08-05 Amersham Pharmacia Biotech Ab Nucleic acid analysis method
DE69926057T2 (en) 1998-02-04 2006-04-20 Amersham Biosciences Corp. DIDESOXY COLOR TERMINATORS
US6150147A (en) 1998-02-06 2000-11-21 Affymetrix, Inc. Biological array fabrication methods with reduction of static charge
IL123256A0 (en) 1998-02-10 1998-09-24 Yeda Res & Dev Methods for dna amplification and sequencing
WO1999044045A1 (en) 1998-02-27 1999-09-02 Massachusetts Institute Of Technology Single molecule detection with surface-enhanced raman scattering and applications in dna or rna sequencing
CA2324248A1 (en) 1998-03-18 1999-09-23 Amersham Pharmacia Biotech Inc. Thermostable dna polymerase from thermoanaerobacter thermohydrosulfuricus
GB9805918D0 (en) 1998-03-19 1998-05-13 Nycomed Amersham Plc Sequencing by hybridisation
US6185030B1 (en) * 1998-03-20 2001-02-06 James W. Overbeck Wide field of view and high speed scanning microscopy
WO1999049082A2 (en) 1998-03-23 1999-09-30 Invitrogen Corporation Modified nucleotides and methods useful for nucleic acid sequencing
US6719868B1 (en) 1998-03-23 2004-04-13 President And Fellows Of Harvard College Methods for fabricating microfluidic structures
DE29806082U1 (en) 1998-04-02 1998-06-18 Ideal Electronics Inc., San Chung, Taipei Cooling device for a central processing unit
WO1999053423A1 (en) 1998-04-16 1999-10-21 Northeastern University Expert system for analysis of dna sequencing electropherograms
US5945325A (en) 1998-04-20 1999-08-31 California Institute Of Technology Thermally stable para-nitrobenzyl esterases
WO1999057321A1 (en) 1998-05-01 1999-11-11 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and dna molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130046A (en) 1998-05-04 2000-10-10 Affymetrix, Inc. Techniques for synthesis integrity evaluation utilizing cycle fidelity probes
US6261848B1 (en) 1998-05-08 2001-07-17 The Johns Hopkins University Miniature immuno-optical rapid analyte sensor platform
WO1999061888A2 (en) 1998-05-22 1999-12-02 California Institute Of Technology Microfabricated cell sorter
JPH11352409A (en) 1998-06-05 1999-12-24 Olympus Optical Co Ltd Fluorescence detector
WO1999064840A1 (en) 1998-06-09 1999-12-16 Caliper Technologies Corp. Fluorescent polarization detection in microfluidic systems
US6287821B1 (en) 1998-06-11 2001-09-11 Orchid Biosciences, Inc. Nucleotide analogues with 3'-pro-fluorescent fluorophores in nucleic acid sequence analysis
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
US6479267B1 (en) 1998-06-17 2002-11-12 Amersham Pharmacia Biotech, Inc. FY7 polymerase
GB9813216D0 (en) 1998-06-18 1998-08-19 Pyrosequencing Ab Reaction monitoring systems
WO2000000637A2 (en) 1998-06-26 2000-01-06 Visible Genetics Inc. Method for sequencing nucleic acids with reduced errors
US6235473B1 (en) 1998-07-02 2001-05-22 Orchid Biosciences, Inc. Gene pen devices for array printing
US7399844B2 (en) 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
EP1105529B2 (en) 1998-07-30 2013-05-29 Illumina Cambridge Limited Arrayed biomolecules and their use in sequencing
US20040106110A1 (en) 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
WO2002061127A2 (en) 2001-01-30 2002-08-08 Solexa Ltd. Arrayed polynucleotides and their use in genome analysis
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US6716394B2 (en) * 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
US6447724B1 (en) 1998-08-11 2002-09-10 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
AU5479599A (en) 1998-08-11 2000-03-06 Caliper Technologies Corporation Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6306643B1 (en) 1998-08-24 2001-10-23 Affymetrix, Inc. Methods of using an array of pooled probes in genetic analysis
AU9505798A (en) 1998-09-24 2000-04-10 Biotraces, Inc. Sequencing duplex dna by mass spectroscopy
DE19844931C1 (en) * 1998-09-30 2000-06-15 Stefan Seeger Procedures for DNA or RNA sequencing
US6458945B1 (en) 1998-10-01 2002-10-01 Variagenics, Inc. Method for analyzing polynucleotides
US6566059B1 (en) 1998-10-01 2003-05-20 Variagenics, Inc. Method for analyzing polynucleotides
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6607888B2 (en) 1998-10-20 2003-08-19 Wisconsin Alumni Research Foundation Method for analyzing nucleic acid reactions
DE19849348A1 (en) * 1998-10-26 2000-04-27 Univ Ludwigs Albert Identification and/or sequencing of an unknown DNA or RNA sequence adjacent to a known DNA or RNA region comprises linker-mediated PCR following amplification by linear PCR
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
RU2143343C1 (en) 1998-11-03 1999-12-27 Самсунг Электроникс Ко., Лтд. Microinjector and microinjector manufacture method
US6309701B1 (en) 1998-11-10 2001-10-30 Bio-Pixels Ltd. Fluorescent nanocrystal-labeled microspheres for fluorescence analyses
US6387982B1 (en) 1998-11-23 2002-05-14 Dentsply Detrey G.M.B.H. Self etching adhesive primer composition and polymerizable surfactants
US6245518B1 (en) 1998-12-11 2001-06-12 Hyseq, Inc. Polynucleotide arrays and methods of making and using the same
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
US6340750B1 (en) * 1998-12-18 2002-01-22 The Texas A&M University System Through bond energy transfer in fluorescent dyes for labelling biological molecules
WO2000037680A1 (en) 1998-12-18 2000-06-29 The Regents Of The University Of California Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
GB9828785D0 (en) 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
DE60042775D1 (en) 1999-01-06 2009-10-01 Callida Genomics Inc IMPROVED SEQUENCING BY HYBRIDIZATION THROUGH THE USE OF PROBABLE MIXTURES
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
WO2000042223A1 (en) 1999-01-15 2000-07-20 Myriad Genetics, Inc. Method for controlling the distribution of dna sequencing termination products
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
US6270644B1 (en) 1999-01-27 2001-08-07 Affymetrix, Inc. Capillary array electrophoresis scanner
US6632655B1 (en) 1999-02-23 2003-10-14 Caliper Technologies Corp. Manipulation of microparticles in microfluidic systems
AU3508600A (en) 1999-02-26 2000-09-14 Orchid Biosciences, Inc. Microstructures for use in biological assays and reactions
US6558945B1 (en) 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
AU3174600A (en) 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
JP2003527075A (en) 1999-03-25 2003-09-16 ハイセック，インコーポレーテッド Solution-based method for sequence analysis by hybridization
US6403317B1 (en) 1999-03-26 2002-06-11 Affymetrix, Inc. Electronic detection of hybridization on nucleic acid arrays
EP1165839A2 (en) 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
US6261775B1 (en) 1999-04-09 2001-07-17 The Regents Of The University Of California Detection of chromosome copy number changes to distinguish melanocytic nevi from malignant melanoma
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
US6368562B1 (en) 1999-04-16 2002-04-09 Orchid Biosciences, Inc. Liquid transportation system for microfluidic device
US20030108867A1 (en) 1999-04-20 2003-06-12 Chee Mark S Nucleic acid sequencing using microsphere arrays
US6521428B1 (en) * 1999-04-21 2003-02-18 Genome Technologies, Llc Shot-gun sequencing and amplification without cloning
US20020142329A1 (en) 1999-04-30 2002-10-03 Aclara Biosciences, Inc. Compositions and methods employing cleavable electrophoretic tag reagents
US6673550B2 (en) 1999-04-30 2004-01-06 Aclara Biosciences, Inc. Electrophoretic tag reagents comprising fluorescent compounds
US6395559B1 (en) 1999-05-04 2002-05-28 Orchid Biosciences, Inc. Multiple fluid sample processor with single well addressability
US7655443B1 (en) 1999-05-07 2010-02-02 Siemens Healthcare Diagnostics, Inc. Nucleic acid sequencing with simultaneous quantitation
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6423273B1 (en) 1999-05-19 2002-07-23 Orchid Biosciences, Inc. Method of forming seals for a microfluidic device
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
WO2000071755A2 (en) 1999-05-25 2000-11-30 Praelux Incorporated Method for sequency and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
US6485690B1 (en) 1999-05-27 2002-11-26 Orchid Biosciences, Inc. Multiple fluid sample processor and system
US6225109B1 (en) 1999-05-27 2001-05-01 Orchid Biosciences, Inc. Genetic analysis device
US6309886B1 (en) 1999-06-04 2001-10-30 The Regents Of The University Of California High throughput analysis of samples in flowing liquid
AU5497100A (en) 1999-06-19 2001-01-09 Hyseq, Inc. Improved methods of sequence assembly in sequencing by hybridization
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
EP1065378B1 (en) 1999-06-28 2002-04-03 California Institute of Technology Microfabricated elastomeric valve and pump systems
US6268219B1 (en) 1999-07-09 2001-07-31 Orchid Biosciences, Inc. Method and apparatus for distributing fluid in a microfluidic device
US6448090B1 (en) 1999-07-09 2002-09-10 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using alternating pressure waveforms
US6395232B1 (en) 1999-07-09 2002-05-28 Orchid Biosciences, Inc. Fluid delivery system for a microfluidic device using a pressure pulse
US6444173B1 (en) 1999-07-09 2002-09-03 Orchid Biosciences, Inc. Method of moving and detecting fluid in a microfluidic device
US6444106B1 (en) 1999-07-09 2002-09-03 Orchid Biosciences, Inc. Method of moving fluid in a microfluidic device
AU6095000A (en) 1999-07-13 2001-01-30 Whitehead Institute For Biomedical Research Generic sbe-fret protocol
US20020045182A1 (en) 1999-07-16 2002-04-18 Lynx Therapeutics, Inc. Multiplexed differential displacement for nucleic acid determinations
US6927065B2 (en) 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
AU7105300A (en) * 1999-09-03 2001-04-10 Lifebeam Technologies, Inc. Optical system for rapid polymer analysis
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6107061A (en) 1999-09-18 2000-08-22 The Perkin-Elmer Corporation Modified primer extension reactions for polynucleotide sequence detection
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
GB9923324D0 (en) 1999-10-01 1999-12-08 Pyrosequencing Ab Separation apparatus and method
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6077674A (en) 1999-10-27 2000-06-20 Agilent Technologies Inc. Method of producing oligonucleotide arrays with features of high purity
EP1096024A1 (en) 1999-10-28 2001-05-02 Remacle, José Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays
EP1228244A4 (en) 1999-11-04 2005-02-09 California Inst Of Techn METHOD AND APPARATUS FOR THE ANALYSIS OF POLYNUCLEOTIDE SEQUENCES
EP1232270A1 (en) 1999-11-23 2002-08-21 Amersham Biosciences Corp. IMPROVING DIDEOXYNUCLEOTIDE-TRIPHOSPHATE UTILIZATION BY THE HYPER-THERMOPHILIC DNA POLYMERASE FROM THE ARCHAEON i PYROCOCCUS FURIOSUS /i
WO2001038574A1 (en) 1999-11-26 2001-05-31 Bioneer Corporation Dna sequencing method which employs various dna polymerases and kit used for the same
US6383749B2 (en) 1999-12-02 2002-05-07 Clontech Laboratories, Inc. Methods of labeling nucleic acids for use in array based hybridization assays
GB9929381D0 (en) 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
WO2001048184A2 (en) 1999-12-23 2001-07-05 Axaron Bioscience Ag Method for carrying out the parallel sequencing of a nucleic acid mixture on a surface
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
AU3839101A (en) 2000-02-15 2001-08-27 Lynx Therapeutics, Inc. Data analysis and display system for ligation-based dna sequencing
US6632645B1 (en) 2000-03-02 2003-10-14 Promega Corporation Thermophilic DNA polymerases from Thermoactinomyces vulgaris
JP4323055B2 (en) 2000-03-22 2009-09-02 富士通マイクロエレクトロニクス株式会社 Semiconductor device testing contactor and method of manufacturing the same
WO2001075154A2 (en) 2000-04-03 2001-10-11 Axaron Bioscience Ag Novel method for the parallel sequencing of a nucleic acid mixture on a surface
US6436641B1 (en) 2000-04-17 2002-08-20 Visible Genetics Inc. Method and apparatus for DNA sequencing
AU2001270504A1 (en) 2000-05-04 2001-11-12 Syngenta Participations Ag Novel assay for nucleic acid analysis
US6342326B1 (en) * 2000-05-10 2002-01-29 Beckman Coulter, Inc. Synthesis and use of acyl fluorides of cyanine dyes
WO2001085991A2 (en) 2000-05-12 2001-11-15 Gnothis Holding Sa Method for detecting polynucleotides using fluorescence correlation spectroscopy
GB0013276D0 (en) 2000-06-01 2000-07-26 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US20020168678A1 (en) 2000-06-07 2002-11-14 Li-Cor, Inc. Flowcell system for nucleic acid sequencing
US6869764B2 (en) 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
ATE423221T1 (en) 2000-06-13 2009-03-15 Univ Boston USE OF MASS-MATCHED NUCLEOTIDES IN THE ANALYSIS OF OLIGONUCLEOTIDE MIXTURES AND IN HIGH-MULTIPLEX NUCLEIC ACID SEQUENCING
WO2002000343A2 (en) 2000-06-27 2002-01-03 Fluidigm Corporation A microfluidic design automation method and system
DE10031842A1 (en) 2000-06-30 2002-01-31 Gnothis Holding Sa Ecublens Multiplex sequencing method
GB0016258D0 (en) 2000-07-03 2000-08-23 Amersham Pharm Biotech Uk Ltd Base analogues
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
JP2004513619A (en) 2000-07-07 2004-05-13 ヴィジゲン　バイオテクノロジーズ　インコーポレイテッド Real-time sequencing
US20030017461A1 (en) * 2000-07-11 2003-01-23 Aclara Biosciences, Inc. Tag cleavage for detection of nucleic acids
US6397150B1 (en) 2000-07-27 2002-05-28 Visible Genetics Inc. Method and apparatus for sequencing of DNA using an internal calibrant
AU3511802A (en) 2000-08-22 2002-05-06 Affymetrix, Inc. System method, and computer software product for controlling biological microarray scanner
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
JP3638516B2 (en) 2000-09-28 2005-04-13 株式会社日立製作所 Nucleic acid detection method and nucleic acid detection kit
US6835537B1 (en) 2000-09-29 2004-12-28 Myriad Genetics, Inc. Method for equalizing band intensities on sequencing gels
EP1322936A2 (en) 2000-10-03 2003-07-02 California Institute Of Technology Microfluidic devices and methods of use
US6664079B2 (en) 2000-10-06 2003-12-16 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP1336097A4 (en) 2000-10-13 2006-02-01 Fluidigm Corp SAMPLE INJECTION SYSTEM USING A MICROFLUIDIC DEVICE, FOR ANALYSIS DEVICES
US6448407B1 (en) 2000-11-01 2002-09-10 Pe Corporation (Ny) Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis
WO2002040701A2 (en) 2000-11-08 2002-05-23 The Scripps Research Institute Energy transfer labels with mechanically linked fluorophores
US6770472B2 (en) 2000-11-17 2004-08-03 The Board Of Trustees Of The Leland Stanford Junior University Direct DNA sequencing with a transcription protein and a nanometer scale electrometer
WO2002044414A2 (en) 2000-11-28 2002-06-06 Promega Corporation Purification of dna sequencing reactions using silica magnetic particles
WO2002055997A2 (en) * 2001-01-12 2002-07-18 Karolinska Innovations Ab Substrate for fluorescence analysis
US20020197618A1 (en) 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
US20020102595A1 (en) 2001-01-29 2002-08-01 Davis Lloyd Mervyn Methods for detection of incorporation of a nucleotide onto a nucleic acid primer
AU2002258502A1 (en) 2001-03-12 2002-09-24 Affymetrix, Inc. Nucleic acid labeling compounds
CA2440754A1 (en) 2001-03-12 2002-09-19 Stephen Quake Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20040038206A1 (en) * 2001-03-14 2004-02-26 Jia Zhang Method for high throughput assay of genetic analysis
DE10115309A1 (en) 2001-03-28 2002-10-02 Gnothis Holding Sa Ecublens Microscope arrangement for fluorescence spectroscopy, in particular fluorescence correlation spectroscopy
US20030027140A1 (en) * 2001-03-30 2003-02-06 Jingyue Ju High-fidelity DNA sequencing using solid phase capturable dideoxynucleotides and mass spectrometry
DE10120798B4 (en) 2001-04-27 2005-12-29 Genovoxx Gmbh Method for determining gene expression
DE10120797B4 (en) 2001-04-27 2005-12-22 Genovoxx Gmbh Method for analyzing nucleic acid chains
CN1384203A (en) 2001-04-30 2002-12-11 香港科技创业股份有限公司 DNA Low Temperature Cyclic Extension Reaction Method with High Extension Specificity
US20020172948A1 (en) 2001-05-04 2002-11-21 Perlin Mark W. Method and system for nucleic acid sequencing
US20050260570A1 (en) 2001-05-29 2005-11-24 Mao Jen-I Sequencing by proxy
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US6689478B2 (en) * 2001-06-21 2004-02-10 Corning Incorporated Polyanion/polycation multilayer film for DNA immobilization
US6613523B2 (en) * 2001-06-29 2003-09-02 Agilent Technologies, Inc. Method of DNA sequencing using cleavable tags
US6989267B2 (en) * 2001-07-02 2006-01-24 Agilent Technologies, Inc. Methods of making microarrays with substrate surfaces having covalently bound polyelectrolyte films
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US6995841B2 (en) * 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
US7223541B2 (en) 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
AU2002337030A1 (en) 2001-08-29 2003-03-18 Genovoxx Gmbh Method for analyzing nucleic acid sequences and gene expression
US20030104386A1 (en) 2001-08-31 2003-06-05 The Regents Of The University Of California Methods for the specific detection of redox-active tags and the use thereof for capillary gel electrophoresis and DNA sequencing
EP1438435B1 (en) * 2001-08-31 2007-10-31 Datascope Investment Corp. Methods for blocking nonspecific hybridisations of nucleic acid sequences
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6975943B2 (en) 2001-09-24 2005-12-13 Seqwright, Inc. Clone-array pooled shotgun strategy for nucleic acid sequencing
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
WO2003031947A2 (en) 2001-10-04 2003-04-17 Genovoxx Gmbh Device for sequencing nucleic acid molecules
US20040054162A1 (en) * 2001-10-30 2004-03-18 Hanna Michelle M. Molecular detection systems utilizing reiterative oligonucleotide synthesis
JP3888999B2 (en) 2001-11-22 2007-03-07 株式会社日立製作所 Information processing system using base sequence related information
JP4163622B2 (en) 2001-11-22 2008-10-08 株式会社日立製作所 Information processing method, information processing apparatus, information processing program, and recording medium on which information processing program is recorded
GB0128526D0 (en) 2001-11-29 2002-01-23 Amersham Pharm Biotech Uk Ltd Nucleotide analogues
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0200938D0 (en) 2002-01-16 2002-03-06 Solexa Ltd Prism design for scanning applications
US7211382B2 (en) 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
US20040014096A1 (en) * 2002-04-12 2004-01-22 Stratagene Dual-labeled nucleotides
US7108976B2 (en) 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
DE10256898B4 (en) 2002-11-29 2006-01-12 Senslab-Gesellschaft Zur Entwicklung Und Herstellung Bioelektrochemischer Sensoren Mbh Electrochemical detection of nucleic acids
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
CA2515938A1 (en) * 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
US20050170367A1 (en) 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
DE602005020421D1 (en) 2004-02-19 2010-05-20 Helicos Biosciences Corp METHOD FOR THE ANALYSIS OF POLYNUCLEOTIDE SEQUENCES
US20050239085A1 (en) 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0481065A1 (en) * 1990-05-07 1992-04-22 Stanford Res Inst Int PROCESS OF HYBRIDIZATION AND AMPLIFICATION OF NUCLEIC ACID.
US5976842A (en) * 1997-10-30 1999-11-02 Clontech Laboratories, Inc. Methods and compositions for use in high fidelity polymerase chain reaction
WO2002044425A2 (en) * 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2004074503A2 (en) * 2003-02-21 2004-09-02 Hoser Mark J Nucleic acid sequencing methods, kits and reagents

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BRASLAVSKY IDO ET AL: "Sequence information can be obtained from single DNA molecules.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 1 APR 2003, vol. 100, no. 7, 1 April 2003 (2003-04-01), pages 3960 - 3964, XP002388726, ISSN: 0027-8424 *
RONAGHI M ET AL: "Real-time DNA sequencing using detection of pyrophosphate release.", ANALYTICAL BIOCHEMISTRY. 1 NOV 1996, vol. 242, no. 1, 1 November 1996 (1996-11-01), pages 84 - 89, XP002388725, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
EP1848829B1 (en) 2012-03-07
EP1848829A2 (en) 2007-10-31
CA2599477A1 (en) 2006-08-03
US20060172313A1 (en) 2006-08-03
WO2006081583A2 (en) 2006-08-03
US7482120B2 (en) 2009-01-27
ATE548466T1 (en) 2012-03-15

Similar Documents

Publication Publication Date Title
WO2006081583A3 (en) Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
WO2007005645A3 (en) Materials and methods for the generation of fully 2'-modified nucleic acid transcripts
WO2005118806A3 (en) METHODS AND COMPOSITIONS INVOLVING MicroRNA
WO2010034024A3 (en) Protected monomers and methods of deprotection for rna synthesis
WO2007112263A3 (en) Monomer compositions for the synthesis of rna, methods of synthesis, and methods of deprotection
WO2010018563A3 (en) Compositions and methods for the prognosis of lymphoma
WO2005045015A3 (en) Polymerase
WO2005059096A3 (en) Methods for high fidelity production of long nucleic acid molecules with error control
WO2009006297A3 (en) Methods for altering the genome of a monocot plant cell
WO2008094599A3 (en) Modified rna ligase for efficient 3' modification of rna
WO2010077740A3 (en) Novel antiviral compounds, compositions, and methods of use
WO2008073960A3 (en) Oligonucleotides containing high concentrations of guanine monomers
WO2008017074A3 (en) Dyes and precursors and conjugates thereof
WO2007136736A3 (en) Methods for nucleic acid sorting and synthesis
WO2008042289A3 (en) Polymer synthetic technique
WO2008098180A3 (en) Complementary personal lubrificant compositions and delivery system
WO2008011433A3 (en) Styrenated terpene resin as well as methods of making and using the same
WO2009010251A3 (en) Mutant dna polymerases and related methods
WO2008073863A3 (en) Preparation and utility of substituted allylamines
WO2007115929A8 (en) Thiazolyl-dihydroquinazolines
TW200702387A (en) Polycarbonate resin composition having good chemical resistance and flowability
WO2007130967A3 (en) Novel oligonucleotide primers and methods for dna replication
WO2008151636A8 (en) Synthesis of mycophenolic acid (mpa)
WO2010058393A3 (en) Compositions and methods for the prognosis of colon cancer
WO2007127309A3 (en) Processes for the synthesis of 3-isobutylglutaric acid

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2006734692

Country of ref document: EP

ENP Entry into the national phase

Ref document number: 2599477

Country of ref document: CA