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WO2005116245B1 - Production d'adn complementaire a partir de cellules apres microdissection au laser - Google Patents

Production d'adn complementaire a partir de cellules apres microdissection au laser

Info

Publication number
WO2005116245B1
WO2005116245B1 PCT/DE2005/000979 DE2005000979W WO2005116245B1 WO 2005116245 B1 WO2005116245 B1 WO 2005116245B1 DE 2005000979 W DE2005000979 W DE 2005000979W WO 2005116245 B1 WO2005116245 B1 WO 2005116245B1
Authority
WO
WIPO (PCT)
Prior art keywords
lysis
cdna synthesis
cells
indicates
biological sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/DE2005/000979
Other languages
German (de)
English (en)
Other versions
WO2005116245A3 (fr
WO2005116245A2 (fr
Inventor
Birgit Liss
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Philipps Universitaet Marburg
Original Assignee
Philipps Universitaet Marburg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Philipps Universitaet Marburg filed Critical Philipps Universitaet Marburg
Priority to US11/569,741 priority Critical patent/US7915016B2/en
Priority to AT05755065T priority patent/ATE460501T1/de
Priority to EP05755065A priority patent/EP1753877B1/fr
Priority to DE502005009197T priority patent/DE502005009197D1/de
Publication of WO2005116245A2 publication Critical patent/WO2005116245A2/fr
Publication of WO2005116245A3 publication Critical patent/WO2005116245A3/fr
Publication of WO2005116245B1 publication Critical patent/WO2005116245B1/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un nouveau procédé pour produire de l'ADN complémentaire à partir de cellules individuelles après une microdissection. Cette invention est avantageuse en ce qu'elle est économique et en ce qu'elle peut être mise en oeuvre rapidement en peu d'étapes de travail et ce, même par du personnel de laboratoire peu qualifié. Lors de la production d'ADN complémentaire à partir de cellules individuelles, l'étape coûteuse et risquée d'isolation d'ARN n'est plus nécessaire, en ce que la lyse et la synthèse d'ADN complémentaire ont lieu dans le même récipient de réaction dans une solution tampon qui donne des résultats plus sûrs et non contaminés. La composition du tampon est constituée de NP40, d'ARN porteur, de super ARNsine et de dNTP et d'amorces de synthèse d'ADN complémentaire.
PCT/DE2005/000979 2004-05-28 2005-05-27 Production d'adn complementaire a partir de cellules apres microdissection au laser Ceased WO2005116245A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US11/569,741 US7915016B2 (en) 2004-05-28 2005-05-27 cDNA production from cells after laser microdissection
AT05755065T ATE460501T1 (de) 2004-05-28 2005-05-27 Erfindung betreffend cdna-herstellung aus zellen nach laser-mikrodissektion
EP05755065A EP1753877B1 (fr) 2004-05-28 2005-05-27 Production d'adn complementaire a partir de cellules apres microdissection au laser
DE502005009197T DE502005009197D1 (de) 2004-05-28 2005-05-27 ERFINDUNG BETREFFEND cDNA-HERSTELLUNG AUS ZELLEN NACH LASER-MIKRODISSEKTION

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004026744A DE102004026744A1 (de) 2004-05-28 2004-05-28 Erfindung betreffend cDNA-Herstellung aus Zellen nach Laser-Mikrodissektion
DE102004026744.8 2004-05-28

Publications (3)

Publication Number Publication Date
WO2005116245A2 WO2005116245A2 (fr) 2005-12-08
WO2005116245A3 WO2005116245A3 (fr) 2006-03-23
WO2005116245B1 true WO2005116245B1 (fr) 2006-05-11

Family

ID=34971159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2005/000979 Ceased WO2005116245A2 (fr) 2004-05-28 2005-05-27 Production d'adn complementaire a partir de cellules apres microdissection au laser

Country Status (5)

Country Link
US (1) US7915016B2 (fr)
EP (1) EP1753877B1 (fr)
AT (1) ATE460501T1 (fr)
DE (2) DE102004026744A1 (fr)
WO (1) WO2005116245A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2365447T3 (es) 2007-05-03 2011-10-05 Roche Diagnostics Gmbh Cuantificación del arnm de células individuales con rt-pcr en tiempo real.
WO2010012464A1 (fr) 2008-08-01 2010-02-04 Roche Diagnostics Gmbh Lyse et transcription inverse améliorées pour la quantification d'arnm
KR101420094B1 (ko) * 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
CN105755545B (zh) * 2010-12-27 2019-05-03 艾比斯生物科学公司 核酸样品的制备方法及组合物
CN103687961B (zh) * 2011-05-06 2022-11-22 赛雷纳（中国）医疗科技有限公司 用于等温全基因组扩增的方法和组合物
WO2020146037A1 (fr) 2019-01-09 2020-07-16 Google Llc Machine de microdissection par capture laser à réalité augmentée

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2085246T1 (es) * 1991-12-02 1996-06-01 Synaptic Pharma Corp Adn que codifica un receptor humano 5-ht1e y utilizaciones del mismo.
DE69434166T2 (de) * 1993-09-20 2005-11-10 Novartis Ag Menschliche metabotropische glutamatrezeptor untertype hmglur7 und verwandte dns-verbindungen
WO1997029354A1 (fr) 1996-02-05 1997-08-14 Bayer Aktiengesellschaft Procede et dispositif pour trier et recuperer des objets biologiques deposes sur un support plat, tels que des cellules biologiques ou des organites cellulaires, des coupes histologiques, des particules de chromosomes etc., au moyen de faisceaux laser
US6589750B2 (en) * 1997-02-20 2003-07-08 Institut Pasteur Therapeutic use of the SMR1 protein, the SMR1 maturation products, specifically the QHNPR pentapeptide as well as its biologically active derivatives
EP1290029A2 (fr) * 2000-06-07 2003-03-12 Chiron Corporation Compositions et methodes de traitement de maladies neoplastiques par usage de modulateurs de net-4
US20030166190A1 (en) * 2001-12-10 2003-09-04 Wright David A. Nucleic acids related to plant retroelements
US20040022727A1 (en) * 2002-06-18 2004-02-05 Martin Stanton Aptamer-toxin molecules and methods for using same
US7250270B2 (en) * 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction

Also Published As

Publication number Publication date
DE502005009197D1 (de) 2010-04-22
EP1753877A2 (fr) 2007-02-21
WO2005116245A3 (fr) 2006-03-23
DE102004026744A1 (de) 2005-12-29
EP1753877B1 (fr) 2010-03-10
US7915016B2 (en) 2011-03-29
US20080261275A1 (en) 2008-10-23
ATE460501T1 (de) 2010-03-15
WO2005116245A2 (fr) 2005-12-08

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