WO2005036965A1 - Insecticide - Google Patents

Abstract

[PROBLEMS] To provide an insecticide showing a high insecticidal activity at a low concentration of the active ingredient and being excellent in persistence of the insecticidal activity. [MEANS FOR SOLVING PROBLEMS] An insecticide characterized by containing a fatty acid glyceride having from 8 to 22 carbon atoms and a macrolide-type insecticide.

Description

 Specification

 Insecticide

 Technical field

 [0001] The present invention relates to an insecticide, and more particularly, to an insecticide having an excellent control effect and sustainability against insects particularly difficult to control in the agricultural and horticultural fields.

 Background art

 [0002] Macrolide compounds produced by soil actinomycetes are widely used as insect control agents because they exhibit insecticidal activity at a low concentration on a wide range of insects. Typical macrolides currently in use include spinosad (trade name: spinoace), emamectin benzoate (trade name: afarm), milbemectin (trade name: colomite), and nemadectin (trade name) Name: Mega Top). However, although the insecticidal activity is wide, it has been reported that some compounds have some selectivity in insecticidal activity (for example, see Non-Patent Document 1).

 [0003] Non-Patent Document 1: "Plant Protection", Vol. 54, No. 9 (2001), P377 Also, to date, the sustainability of the control effect of starch and spinosad or emammethatin benzoate has been investigated. Excellent insecticides' acaricides are known. (For example, see Patent Document 1).

 [0004] Patent Document 1: Japanese Patent Application Laid-Open No. 2001-302416 (Claim 1) Each of these insecticides' acaricides can improve the insecticidal activity and maintain its sustainability by mixing them at normal concentrations. The intended drug. However, in practice, there is a problem that insecticidal power is weak for insect pests flying after spraying and larvae hatched after spraying.

 Disclosure of the invention

 Problems to be solved by the invention

It is an object of the present invention to solve such a conventional problem and to provide an insecticide which exhibits high insecticidal activity at a low active ingredient concentration and has excellent persistence of the insecticidal activity. .

 Means for solving the problem

[0006] In order to solve the above-mentioned problems, the present inventors have studied components constituting an insecticide. As a result, a mixture of a fatty acid glyceride, which originally has a low insecticidal activity by itself, and a macrolide insecticide alone as a component, surprisingly has a pesticidal activity and a resistance to Z or its persistence. As a result, the inventors have found that the above problem can be solved, and have completed the present invention based on this finding.

 [0007] That is, means of the present invention for solving the above problems of the present invention include:

 An insecticide characterized by containing a fatty acid glyceride obtained from a fatty acid having 8 to 22 carbon atoms and glycerin (hereinafter, may be simply referred to as a fatty acid glyceride) and a macrolide-based insecticide. It is.

[0008] In a preferred aspect of the invention for solving the above problems, the following (

1) One (3) insecticides.

 (1) An insecticide wherein the fatty acid forming the fatty acid glyceride is a plant-derived fatty acid.

(2) An insecticide wherein the concentration of the fatty acid glyceride is from 200 to 3000 ppm and the concentration of the macrolide insecticide is from 0.2 to 100 ppm.

 (3) An insecticide for controlling pests of the second order, pests of the order Hemiptera, pests of the order Thysanoptera or pests of the order Lepidoptera.

 The invention's effect

 [0009] According to the present invention, a fatty acid having a low concentration of a fatty acid having 8 to 22 carbon atoms can be obtained with respect to a pest which does not originally show a pesticidal activity with a macrolide insecticide or a pest which is ineffective at a low concentration. By mixing acid glyceride and macrolide insecticide, a specific insecticidal action was observed in each process of growing the target pest from egg to adult. In other words, after spraying, surviving adults and laid eggs have an oviposition effect, an oviposition suppression effect, a feeding cessation effect, and an improved insecticidal effect, resulting in a high pest control effect. Thus, the contribution to pest control in the agricultural and horticultural fields is extremely large.

 BEST MODE FOR CARRYING OUT THE INVENTION

[0010] The present invention is an insecticide containing a fatty acid glyceride and a macrolide insecticide.

The fatty acid glyceride used in the present invention is a glycerin ester of a fatty acid having 8 to 22 carbon atoms and glycerin. The fatty acid forming the fatty acid glyceride may be a natural fatty acid or a synthetic fatty acid, and is derived from a plant, for example, soybean, cottonseed or vegetable. It is preferably a fatty acid derived from a species. Examples of fatty acids having 8 to 22 carbon atoms include capric prillic acid, pelargonic acid, capric acid, pendecanoic acid, lauric acid, tridecanoic acid, myristic acid, pentadecanoic acid, palmitic acid, margaric acid, stearic acid, nonadecanoic acid, and araquinine. Acids, heneicosanoic acids, behenic acids and the like can be mentioned, and among them, caprylic acid, capric acid and mixtures thereof are preferred.

[0012] The fatty acid glyceride can be produced from the fatty acid and glycerin by esterification or transesterification according to a conventional method. Fatty acid glycerides to be produced include fatty acid monoglyceride, fatty acid diglyceride and fatty acid triglyceride, and any fatty acid glyceride can be used. Among them, glyceride (decanoyl otatanyl glycerol, trade name: sun crystal emulsion) of a mixed fatty acid of force prillic acid and force pric acid is preferable.

 [0013] As the macrolide insecticide used in the present invention, insecticides having the power of a macrolide compound produced by soil actinomycetes, for example, spinosad (trade name: spinoace), emma mectin benzoate (trade name: Affirm), millbemectin (trade name: colomite) or nemadectin (trade name: Megatop), and the like.

[0014] The insecticide of the present invention can be prepared, for example, by mixing the fatty acid glyceride and the macrolide insecticide. In the preparation, when the fatty acid glyceride is a solid at room temperature, it is heated and melted to form a liquid substance. The solvent can dissolve the fatty acid glyceride and can be mixed with the macrolide-based insecticide. A solution or suspension may be prepared by mixing with glyceride, and this solution may be mixed with a macrolide-based insecticide. Examples of the solvent include known solvents capable of dissolving fatty acid glyceride, and specifically, a solvent such as methanol, ethanol, isopropanol, propylene glycol, glycerin, sorbitol, or a mixed solvent thereof. Can be mentioned. When the fatty acid glyceride is liquid at room temperature, it is preferable to mix and prepare the fatty acid glyceride and the macrolide insecticide at room temperature or while heating. In preparing the mixture, an emulsifier, a dispersant, an auxiliary agent, and the like known in the technical field of the insecticide can be appropriately added to and mixed with the fatty acid glyceride and the macrolide-based insecticide. [0015] The particle size of the macrolide insecticide used in this case is preferably granular, and there is no particular limitation on the particle size of the macrolide insecticide when used in a granular shape. The conditions for mixing the macrolide insecticide particles are also not limited.

 [0016] The mixing ratio of the fatty acid glyceride and the macrolide insecticide is not particularly limited.

 [0017] The insecticide of the present invention can be produced by adding an auxiliary agent such as an emulsifier, a dispersant and a fixing agent to a fatty acid glyceride and a macrolide insecticide.

 [0018] The insecticide of the present invention thus produced is preferably diluted with 1S water or the like, which can be used as it is, and then used for spraying or the like.

 In the dilution, a fatty acid glyceride diluted by adding water or the like and a macrolide insecticide diluted by adding water or the like may be mixed. There is no limit on the degree of dilution. The concentration of the fatty acid glyceride in the insecticide thus diluted is usually from 200 to 300 Oppm, and the concentration of the macrolide-based insecticide is usually from 0.2 to 100 ppm.

 [0020] The target pests to be controlled by the insecticide of the present invention include: myxoids (citrus red mite, apple mite, Nami nada, kanzada nada, etc.), and other aphid pests and aphids (pita aphid, peach) Hemiptera pests such as aca aphids, etc., whiteflies (whiteflies, silverleaf whiteflies, tobacco whiteflies, etc.), and thrips (small blue thrips, orange thrips, yellow thrips, etc.) And other lepidopteran pests such as Pterodactyla and Pterodactyla.

[0021] The insecticide of the present invention has a specific insecticidal action against each of these pests in each process of growing into an egg-forming adult, and after spraying, surviving adults and spawning. Egg killing action, egg-laying inhibitory action, feeding cessation action, and improvement in adulticidal activity are observed for eggs that have been broken. In particular, at a low concentration of 0.2 to 100 ppm, a macrolide insecticide which does not originally exhibit insecticidal activity exhibits a high insect pest control action by being mixed with a fatty acid glyceride.

 Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited by these Examples.

Example Examples (Examples of producing insecticides)

 The sun crystal containing 90% decanoyl otatanyl glycerol as an active ingredient (commercial product; trade name; dosage form is emulsion) is diluted by adding water to the dilution ratio shown in the following table. A sun crystal diluent was prepared. A spinoace diluent was prepared by adding water to Spinoace (commercial product; trade name; dosage form is water dispersible granule) containing 25% of spinosad as an active ingredient in the dilution ratio shown in the table below. Was prepared. Next, the sun crystal diluent and the spinoace diluent were mixed to produce an insecticide.

 A dilute solution of sun crystal was prepared by adding and diluting water to the sun crystal so as to have a dilution factor shown in the following table, and water was added to an afarm formulation so as to have a dilution factor shown in the table below. The resulting mixture was mixed with an afarm diluent prepared by dilution to prepare an insecticide.

 A sun crystal diluent prepared by adding water to a sun crystal so as to have a dilution factor shown in the following table, and diluting the water with a colomite emulsion so as to have a dilution factor shown in the following table. The resultant was mixed with a colomite diluent prepared by adding and diluting to produce an insecticide.

 The sun crystal diluent was prepared by diluting the sun crystal with water to obtain the dilution factor shown in the following table, and the megatop solution was diluted to the dilution factor shown in the following table. An insecticide was produced by mixing with a megatop diluent prepared by adding and diluting water.

Comparative Example 1 (Production example of insecticide)

A sun crystal diluent obtained by diluting the sun crystal with water at a dilution multiple shown in the following table was used as an insecticide, and this insecticide was indicated as "Comparative Example 1" in the table. A dilute solution of Santalistal prepared by adding water to the sun crystal to make the dilution shown in the following table, and water to the cascade emulsion by adding water to make the dilution shown in the following table. The insecticide was manufactured by mixing with a cascade diluent prepared by dilution. This insecticide is indicated as “Comparative Example 1 + K” in the table. The Sun Crystal Dilution was prepared by diluting the Sun Crystal with water to the dilution ratio shown in the following table. The diluent was mixed with a Dantotsu diluent prepared by diluting water by adding water to a Dantotsu water solvent at a dilution factor shown in the following table to produce an insecticide. This insecticide is indicated as “Comparative Example 1 + D” in the table. The sun crystal diluent prepared by adding water to the sun crystal to obtain the dilution factor shown in the following table and diluting it, and adding the dilution ratio shown in the following table to the ointane water dispersible powder. An insecticide was produced by mixing with an otran diluted solution prepared by diluting with water. This insecticide is indicated as “Comparative Example 1 + R” in the table.

 Comparative Example 2

 A spinoace diluent obtained by diluting Spinoace with water at a dilution multiple shown in the following table was used as an insecticide, and this insecticide was indicated as "Comparative Example 2" in the table. A mixture of the spinoace diluent and an adhesive diluent obtained by diluting the adhesive liquid with water so as to have a dilution factor shown in the table was used as an insecticide. Example 2 + N ". A mixture of the above spinoace diluent and a dilute solution obtained by diluting a dilute solution with water so as to have a dilution factor shown in the table was used as an insecticide. "Was displayed. A mixture of the spinoace diluent and an acalyche diluent obtained by diluting an acalycate emulsion with water so as to have a dilution factor shown in the table was used as an insecticide. A ".

 Comparative Example 3

The affirm preparation was diluted by adding water so as to have a dilution factor shown in the following table. The prepared affirm diluent was used as the insecticide, and this was indicated as "F" in the table. A colomite diluent prepared by diluting the colomite emulsion with water so as to have a dilution factor shown in the following table was used as an insecticide, and this was indicated as "C" in the table. A cascade diluent prepared by adding water to the cascade emulsion and diluting to the dilution ratio shown in the following table was used as an insecticide, and this was indicated as "K" in the table. Dantot diluent prepared by adding water to Dantot wettable powder and diluting to the dilution ratio shown in the following table was used as an insecticide, and this was indicated as "D" in the table. Ortolan diluent prepared by diluting Ortolan wettable powder with water so as to have a dilution factor shown in the following table was used as an insecticide, and this was indicated as "R" in the table. Mega top The megatop diluent prepared by diluting the solution with water so as to have a dilution factor shown in the following table was used as an insecticide, and this was indicated as "M" in the table. An adhesive liquid diluted by diluting the adhesive liquid with water so as to have a dilution factor shown in the table was defined as an insecticide, and this insecticide was indicated as "N" in the table. The oleate diluent obtained by diluting the oleate solution with water so as to have the dilution factor shown in the table was used as the insecticide, and this insecticide was indicated as "0" in the table. Acacia touch diluent obtained by diluting the acacia touch emulsion with water so as to have the dilution factor shown in the table was used as an insecticide, and this insecticide was indicated as `` A '' in the table.

[0026] Evaluation test 1-6

 Two days after female adult colonies were released, the number of parasites was examined on the leaves of potted cucumber (variety: Honor, 1.5-leaf stage), and the dilution factor shown in Table 1 was obtained. The insecticide diluted in was sprayed with a small atomizer. One day after spraying, the survival status of female adults was investigated. The results are shown in Table 1.

 [Table 1]

[0028] As shown in Table 1, the results of Comparative Examples 1 and 2 indicate that the effect of Spinoace on adult female Nami-Nada (Nami-Nada) is disadvantageous in that it has a large number of paralytic insects and is fast-acting. The power was low. On the other hand, in the case of Sun Crystal Emulsion, the insecticidal activity with a large number of live insects was not high. However, it was recognized that the combination of spinoace and sun crystal improved the immediate efficacy and the adulticidal power. This test shows the immediate effect on adults one day after application. Force Usually, parasites separate into live and dead in 3-4 days. Even in the paralyzed state, they have the ability to lay eggs depending on their degree, so they are Is very important.

 [0029] Evaluation test 7—12

 The insecticides shown in Table 2 were sprayed at a dilution factor (dilution with water) shown in Table 2 on a kidney bean (cultivar: Sakimidori No. 2, first bileaf stage) in pot cultivation. After spraying, the plants were maintained in a glass vine and a mouse. One day and four days after spraying, the first double leaf of the kidney beans was released with 20 adult females of Nami-nada (Pinus aegypti). Three days after release, the number of surviving female adults and the total number of eggs laid were examined. Table 2 shows the results.

 [Table 2]

[0031] The number in parentheses is the number of eggs laid per living insect.

[0032] The results of the examples and comparative examples in Table 2 show that by mixing spinoace and suncrystals to increase the power, the insecticidal power is improved and the number of eggs laid is reduced as compared to the case where they are used alone. A decrease was observed. This tendency is maintained for insects released 4 days after spraying, and improvement in residual efficacy can be expected. After spraying, the number of eggs released by the released adults in 3 days is 22 to 24 eggs per head in the non-sprayed area, 19 to 23 with single use of spinoace, and 115 to 116 with single use of sun crystal. It became. In the mixed plot, the number of spawned eggs was 418 including the low concentration plot.After spraying, whether the released adults came into contact with the insecticide-attached sites on the leaves or caused by poisoning by sucking. Although uncertain, the number of dead solids was high in 3 days, the number of live solids laid was also reduced, and the combined use clearly reduced the number of laid eggs. Control aspects The ability of the mites on the back of the leaf, which is hardly affected by the insecticide, and the mites flying by the wind after spraying, comes into contact with the insecticide-treated leaves. is important.

 [0033] Evaluation test 13—19

 Twenty female female adults of Nami-Nada are released from pot-cultivated kidney beans (cultivar: Sakimidori No. 2, 1st compound leaf stage) at a time, and after laying eggs in a glass house for 2 days, the insecticides shown in Table 3 are applied. It was sprayed at a dilution factor (dilution with water) shown in Table 3 using a small atomizer. Seven days after application, the number of parasitoids was examined at each stage of development. Table 3 shows the results.

 [0034] [Table 3]

[0035] As shown in Table 3, the single use of spinoace granule wettable powder and sun crystal emulsion alone showed an insecticidal activity, but a large number of nymphs that grew from hatched larvae were observed. By mixing Spinoace and Sun Crystal, the ovicidal power was improved and the growth of hatched larvae was stopped. Table 4 shows the growth stages under the same conditions as Kanami-Nada and Kanza-Nada at 27 ° C.

つまり、産卵から成虫までの日数は 10日間である。この試験では、成虫と 2日間産 卵させた卵を対象として散布した。散布後、生存している成虫は産卵し続ける。卵か らふ化した幼虫（足が 6本）は、第 1静止期に入り、脱皮して若虫（足が 8本）に成育す る。各単用区からふ化した幼虫は正常に成育し、大部分が若虫となった。スピノエー スとサンクリスタルとの混用により、混合区では、ふ化幼虫は摂食阻害によって餓死 固体が見られた。混合区では、ふ化幼虫の生虫が見られるが、後記するように、 日数 が経つに従い餓死する。 [Table 4]

In other words, the number of days from spawning to adulthood is 10 days. In this test, spraying was performed on eggs that had been laid for two days with adults. After application, surviving adults continue to lay eggs. An egg The hatched larvae (6 feet) enter the first stationary phase, molt and grow into nymphs (8 feet). The hatched larvae from each single plot grew normally and became mostly nymphs. Due to the mixed use of spinoace and sun crystals, hatched larvae showed starved solids due to inhibition of feeding in the mixed plot. In the mixed plot, live hatchling larvae are observed, but as described later, they starve to death over time.

 [0038] Evaluation test 20—25

 Twenty female adults of Namihada were released on the primary leaves of pot-grown kidney beans (variety: Sakimidori No. 2). After laying eggs for 2 days, the pesticides shown in Table 5 were sprayed at the concentrations shown in Table 5 using a small atomizer. Five, seven and ten days after spraying, the primary leaves of kidney beans were cut off from each pot and the number of surviving hatched larvae was examined. Table 5 shows the results.

 [Table 5]

[0040] The number in parentheses is the growth inhibition rate.

 Growth inhibition rate = 1 (number of surviving insects in the sprayed area Z number of surviving insects in the unsprayed area) x 100

 [0041] With Spinoace Granular Water Dispersible Powder and Sun Crystal Emulsion alone, there was a tendency for the number of larvae to increase over the days after spraying. However, when spinoace and suncrystal were mixed, many dead animals were observed due to the inhibitory effect on feeding to hatched larvae, and after 10 days, the growth of Namihadada could be completely suppressed.

 [0042] Evaluation test 26—41

10 female female adults of Namihadada were released on the primary leaves of pot-grown kidney beans (variety: Sakimidori No. 2) per leaf, and allowed to lay eggs in a glass house for 3 days. Three days after the release, the insecticides shown in Table 6 were sprayed at a dilution factor (dilution with water) and concentration shown in Table 6 using a small atomizer. The number of parasites 9 days after spraying was also used to calculate the growth inhibition rate. Table 6 shows the results. [Table 6]

[0044] Growth inhibition rate = 1 (number of surviving insects in the sprayed area Z number of surviving insects in the non-sprayed area) x 100

 [0045] When Spinoace and Suncrystal were used alone, a large number of surviving insects were observed. However, the mixture of Spinoace and Suncrystal drastically reduced the number of surviving insects, resulting in a high synergistic effect. Admitted. The mixture concentration (ppm) ratio for achieving this effect was spinoace: sun crystal = 1: 8-1: 250. The perfect inhibition mixture concentration ratio is 1:58.

 [0046] Evaluation test 42—49

 Planter cultivated eggplant (variety: Senryo 6-leaf stage) was inoculated with Namihada-parasitic leaf section. Three days after inoculation, the number of Namidanada-parasites before the application of the insecticide was investigated, and then the insecticide shown in Table 7 was sprayed at a dilution factor shown in Table 6 (dilution with water) using a small atomizer. The number of parasites after 7, 14, 20, and 29 days after spraying was examined for all leaves, and the control efficiency was calculated from the number of each parasite. Table 7 shows the results.

[Table 7] Insecticide dilution factor Parasite control efficiency 7 days after treatment 14 days after 20 days 29 days after (%)

4 2 Comparative Example 2 2500 85 Rating 796 11S4 1830 33. 6

 Test

 4 3 Comparative Example 2 NOACE 5000 81 118 520 81S 1684 43.9

4 4 Example Example: £ 5000 5,000 82 0 0 39 249 94.9

 Sun Crystal 300

 4 5 Example Suhi. NOACE 5000+ 82 0 0 6 258 95.3

 San Cris Evening 600

 4 6 Example NOACE 5000+ 82 0 0 1 152 97.3

 Sun Crystal 1200

 4 7 Comparative Example 1 Sun Crystal 300 115 22 206 268 786 S3.9

4 8 Comparative Example 1 Sun Crystal 600 89 39 236 490 930 72.4

4 9 No spray 75 91 890 1620 2581

[0048] Control efficiency = (l-Cb∑Tai / Tb∑Cai) X 100

 Cb: Number of insects before spraying insecticide in no-spray area

 Tai: The number of insects at the i-th time after spraying the insecticide in the spray area

 Tb: Number of insects before spraying insecticide in the spray area

 Cai: The number of insects at the i-th time after spraying the insecticide in the non-spray area

[0049] When Spinoace and Suncrystal were used alone, the effect was improved when mixed with Spinoace and Suncrystal, which had inferior effects on Namihada.

[0050] Evaluation test 50—61

 In the first compound leaf of the kidney bean (cultivar: Sakimidori No. 2) grown in a pot, 20 adult female Namihada were released in Z strains at a time, and the eggs were laid in a glass house for 1 day. After investigating the number of two parasites before spraying the insecticide, the insecticides shown in Table 8 were sprayed at a dilution factor (dilution with water) shown in Table 8 using a small atomizer, and the number of parasites 7 days after spraying was investigated. did. The results are shown in Table 8

[Table 8] Evaluation Insecticide Dilution multiple number Before application Number of parasites Survival treatment Test Number of insects Adult nymph Hatchworm Insect ratio

50 0 Comparative Example 2 Shino-Enos 2500 39 9 344 29 382 63.2

5 1 Comparative Example 2 Shino-noise 5000 36 16 536 28 580 96.0

5 2 Example Sue Ace 5000 + 37 0 0 2 2 0.3 Sun Crystal 600

 5 3 Example Suino Electronics 5000 + 36 0 19 17 36 6.0 Sun Crystal 1200

 5 4 Comparative Example 1 Sun Crystal 300 38 12 272 8 292 48.3

5 5 Comparative example 2 Shino-noise 5000 + 36 3 275 8 286 47.4 N N 100

 5 6 N N 100 38 0 213 3 216 48.3

5 7 Comparative example 2 Shino-noise 5000 + 36 2 151 10 163 27.0 0 O O 100

 5 8 O O 100 37 0 191 0 191 31.6

5 9 Comparative Example 2 Sueace 5000 + 38 1 428 5 434 71.9 + A A 1000

 6 0 A A 1000 39 10 491 7 508 84.1

6 1 No spray 37 28 520 56 604 100

[0052] N: adhesive liquid (manufactured by Sumitomo Chemical Co., Ltd., 5% starch)

 O: Olate solution (20% sodium oleate, manufactured by Otsuka Danigaku Co., Ltd.)

 A: Akari touch emulsion (Ishihara Sangyo Co., Ltd., 70% propylene glycol mono fatty acid ester)

 [0053] In Table 8, "Comparative Example 2 + N" for the insecticide means that the insecticide was a mixture of the insecticide prepared in Comparative Example 2 and the adhesive liquid agent. Show. The same applies to the expressions "Comparative Example 2 + 0" for insecticides and "Comparative Example 2 + A" for insecticides.

 [0054] The effect of mixing spinoacet with a sticky agent solution, an oleate solution or an acalyach emulsion, which is a physical control agent, was not recognized. When Spinoace and Suncrystal were used alone, the acaricidal effect was inferior, but when Spinoace and Suncrystal were mixed, many dead solids of hatched larvae were observed, and other It was found that different effects were obtained from similar physical control agents.

 [0055] Evaluation test 62—97

The leaves (1 branch 1 leaf) of the field mandarin orange (Senju mandarin) were collected, washed with water, and then watered, and then 10 adult female Citrus uncles were released per leaf. After laying eggs in a room at 25 ° C for 4 days, female adults were removed and the number of eggs laid was examined. On the same day, the insecticides shown in Table 9 were sprayed at the dilution factor (dilution with water) and concentration shown in Table 9 using a small atomizer. After spraying, place in a room at 25 ° C, Eight days after spraying, the mortality of hatched larvae was calculated. Table 9 shows the results.

[Table 9]

If Spino Ace and Sun Crystal are used alone, the larvalicidal effect is rarely seen. By mixing Spino Ace and Sun Crystal, starvation is achieved. Many dead solids were observed, and a remarkable improvement in the effect on larvae was observed. At first glance, it seems that the overall mortality rate is lower than the growth inhibitory effect and the control effect, but as the number of days increases, the number of dead animals increases. The most characteristic point of the tested ovicidal effect is that no larva hatched by vigorous power mixing does not grow. This effect is different from that of the use of sun crystal alone, in that it appears only when sun crystals are applied to larvae by continuous application.

 [0058] Evaluation test 98—132

 The insecticide shown in Table 10 was sprayed on the primary leaves of pot-cultivated kidney beans (variety: Sakimidori No. 2) at the dilution factor (dilution with water) and concentration shown in Table 10 and placed in the area where Mamehamamo Daribae was generated. did. Fourteen days after spraying, the number of insect pests infested by the beetle fly after spraying was investigated, and the damage inhibition rate was calculated. Table 10 shows the results.

[Table 10]

Evaluation Pesticide Spinoace Sancristal Damage inhibition test Dilution concentration Dilution concentration (%)

 Multiple (ppm) Multiple (ppm)

 9 8 Example 2500 100 300 3000 100

 9 9 600 1500 100

 1 0 0 1200 750 99.5

 1 0 1 2400 375 97.3

 1 0 2 4800 188 56.9

 1 0 3 5000 50 300 3000 100

 1 0 4 600 1500 100

 1 0 5 1200 750 98.9

 1 0 6 2400 375 87.2

 1 0 7 4800 188 42.0

 1 0 8 10000 25 300 3000 98.9

 1 0 9 600 1500 98.4

 1 1 0 1200 750 95.2

 1 1 1 2400 375 85. 1

 1 1 2 4800 188 26.7

 1 1 3 20000 13 300 3000 96.3

 1 1 4 600 1500

 1 1 5 1200 750 95.7

 1 1 6 2400 375 72.9

 1 1 7 4800 188 14.4

 1 1 8 40000 6 300 3000

 1 1 9 600 1500

 1 2 0 1200 750 85.1

 1 2 1 2400 375

 1 2 2 4800 188 8.0

 1 2 3 Comparative example 1 300 3000 31.9

 1 2 4 600 1500 26. 1

 1 2 5 1200 750 24.5

 1 2 6 2400 375 16.0

 1 2 7 4800 188 2.7

 1 2 8 Comparative Example 2 2500 100 21.3

1 2 9 5000 50 Damage prevention rate = (1 number of parasites in the sprayed area Z number of parasites in the non-sprayed area) x 100 After spraying as described above, no effect has been seen on larvae and hatched larvae that infest the parasite. Even when using Sun Crystal alone. It is the same. By mixing spino ace and sun crystal, spawning, but not hatching larvae It was recognized that the effect of reducing the damage was enhanced and a high damage prevention effect was obtained. After spraying, they were placed in a common area where the legumes and flies were found. By mixing spinoace and sun crystals, some dead adults could be seen, but a large number of food scars and eggs could be seen. At the beginning of hatching, the larva dies after hatching. The aftereffect is also long. At present, there are only a few agents that are effective in preventing damage from the bean paste fly.

 [0061] Evaluation test 133—146

 Planter cultivated mini tomatoes (variety: Thai-It Tim 6.5-leaf stage, 10 plants planted) were sprayed with the insecticide shown in Table 11 at the dilution factor shown in Table 11 (dilution with water). Sprayed. Eight days after spraying, the top five leaves (50 leaves in one section) were examined for the degree of damage caused by the bean hammoebari. The damage degree, leaf damage, damage degree and control value were calculated. Table 11 shows the results.

 [Table 11]

[0063] F: Afarm emulsion (1% ememamethine benzoate, manufactured by Syngenta Japan Co., Ltd.) C: Colomite emulsion (1% milbemectin, manufactured by Sankyo Co., Ltd.)

 K: Cascade emulsion (10% flufenoxuron, manufactured by BS Efagro Co., Ltd.) D: Dantotsu water solvent (16% clothia-zine, manufactured by Takeda Pharmaceutical Co., Ltd.)

 R: Ortlan wettable powder (Takeda Pharmaceutical Co., Ltd., 50% acetate)

 Survey leaves: 150

 Damage level

 0: No trace of dive.

 1: Less than 10% of the submerged traces of the total area.

 2: Submergence scar is less than 10-25% of the total area.

 3: Submersion scars are less than 25-50% of the total area.

 4: Indentation marks are 0% or more of the total area.

 Damage = n + 2n + 3n + 4n / 4 X N

 2 3 4

 n: The number of leaves indicated by the degree of damage.

 N: Number of leaves examined.

 Damage value = Damage of untreated zone-Damage of treated zone Z Damage of untreated zone X 100

[0064] It was recognized that a high control effect was obtained by mixing a macrolide insecticide and a sun crystal emulsion.

[0065] Evaluation test 147-159

 The insecticides shown in Table 12 were sprayed at a concentration shown in Table 12 on small nymphs of the Thrips palmi Karny thistle, which were infested in the first compound leaf stage of the kidney beans (cultivar: Sakimidori No. 2) of pot cultivation. Five days after spraying, the survival status of Thrips palmi Karny was examined, and the mortality was calculated. Table 12 shows the results.

[0066] [Table 12] Evaluation Pesticide Concentration Adult Larval Mortality Test (PPm) Live Insect Insect Live Insect Insect (%)

 147 Example Example 25.0 + 0 3 0 38 100

 Sun Crystal 1500

 148 SHINOISE 12.5 + 0 1 0 27 100

 Sun Crystal 750

 149 Sue Ace 6.3 + 0 1 3 25 89.7

 Sun Crystal 375

 150 Sue Ace 3.1 + 0 2 17 31 66. 7

 Sun Crystal 188

 151 Shino-noise 1.6 + 0 0 15 26 63.4

 Santa Listal 94

 152 Comparative Example 2 Shino Ace 25.0 0 3 0 25 100

 153 Shino-noise 12.5 0 2 11 12 56.0

 154 Suino Ace 6.3 0 0 30 6 16.7

 155 Ace 3.1 3 0 48 47.3

 156 Shino-noise 1.6 1 3 40 2 10.9

 157 Comparative Example 1 Sun Crystal 1500 2 4 19 9 38.2

 158 Sun Crystal 750 3 1 31 1 5.6

 159 No spraying 3 2 36 2 9.3

When spinoace is used alone, the effect is inferior. It was recognized that a high control effect was obtained by mixing spinoace and suncrystal in a concentration of 12.5 ppm or less.

 [0068] Evaluation test 160—164

 The primary leaves of pot-grown kidney beans (variety: Toramaru quail) were placed in breeding cages (30 X 25 X 28 cm), and adults of whiteflies were released and allowed to lay eggs for 2 days. After the spawning, the insecticides shown in Table 13 were sprayed at a dilution factor shown in Table 13 (dilution with water) using a small atomizer. Two days after spraying, the parasitic leaves were cut off, and hatching status and the mortality of hatched larvae were examined using a stereoscopic microscope. Table 13 shows the results.

 [Table 13]

 Evaluation Insecticide Effect on diluted multiple eggs Effect on hatched larvae Test on hatchling ovality Adult worms Insects Dead larvae Eggs Eggs (%) (%)

160 Suhi. NOES 2500 141 27 16.1 43 21 44 40.7

161 Example Suhi ^÷ Noesu 25ϋΰ + 154 1 B 9 .4 2 9 109 90.6 thunk y Stal soci

162 Example Suhi ^÷ Noesu 5ΰϋΰ + 140 20 12.5 0 5 131 96.3 thunk y Stal soci

163 Comparative Example 1 Thunk y Star 300 116 19 14 ₊ 1 53 28 25 23, 6

184 No spray 201 20 10, 0 109 0 0 0 [0070] It was recognized that the effect on hatchling larvae was remarkably improved and a stable effect was obtained by mixing spinoace and sun crystal.

[0071] Evaluation test 165—200

 Inoculate the first leaf of a pot lily (cultivar: Shinko No. A) with a parasitic aphid leaf,

One day later, the number of parasites was investigated. After the investigation, the insecticides shown in Table 14 were sprayed at the dilution factor (dilution with water) and concentration shown in Table 14 using a small atomizer. Five days after application, the number of parasites was examined, and the growth inhibition rate was calculated. Table 14 shows the results.

[Table 14]

Evaluation Pesticide Spinoace Sancristal Reproduction inhibition test Dilution concentration Dilution concentration (%)

 Multiple (ppm) Multiple (ppm)

 165 Example 2500 100 300 3000 98

 166 600 1500 99

 167 1200 750 95

 168 2400 375 92

 169 4800 188 67

 170 5000 50 300 3000 100

 171 600 1500 95

 172 1200 750 90

 173 2400 375 81

 174 4800 188 15

 175 10000 25 300 3000 100

 176 600 1500 100

 177 1200 750 88

 178 2400 375 53

 179 4800 188 0

 180 20000 13 300 3000 83

 181 600 1500 60

 182 1200 750 56

 183 2400 375 0

 184 4800 188 0

 185 40000 6 300 3000 40

 186 600 1500 84

 187 1200 750 30

 188 2400 375 3

 189 4800 188 0

 190 Comparative Example 1 300 3000 70

 191 600 1500 69

 192 1200 750 34

 193 2400 375 0

 194 4800 188 0

 195 Comparative Example 2 2500 100 0

 196 5000 50 0

[0073] Growth inhibition rate = (1-number of pre-treated insects in untreated group X number of treated insects in treated group Z number of treated insects in treated group X number of treated insects in untreated group) x 100

[0074] Force that Spinoace had no effect when used alone. By mixing Spinoace and Suncrystal, the growth-inhibiting effect on P. aphid was enhanced. [0075] Evaluation test 201—230

 Twenty adult female Namihada (2 pots) were released onto the primary leaves of pot-grown kidney beans (variety: Saki Midori No. 2). After laying eggs for two days, the insecticides shown in Table 15 were sprayed at the concentrations shown in Table 15 using a small atomizer. Nine days after spraying, the number of surviving insects was examined, and the growth inhibition rate was calculated. Table 15 shows the results.

 [0076] [Table 15]

[0077] Growth inhibition rate C: Colomite emulsion (1% milbemectin, manufactured by Sankyo Co., Ltd.)

 The effect of using colomite alone was inferior. At a concentration of lppm or less, a stable effect was obtained by mixing the colomite emulsion and the sun crystal emulsion. The optimum mixing ratio was colomite: sancristanole = 1000 ppm: l. 13 ppm (1: 885).

 [0078] Evaluation test 231— 238

 The insecticides shown in Tables 16 and 17 were sprayed onto the first true leaves of the pot lilies (cultivar: Shinko A) at a dilution factor (dilution with water) shown in Tables 16 and 17 using a small sprayer. Two, four, six, and eight days after spraying, 15 female female adults were released on the surface of the cucumber leaves. Two days after each release, the number of surviving insects and the number of eggs that laid behind the leaves were determined. The numbers in parentheses are the number of eggs laid per animal. The results are shown in Tables 16 and 17.

 [0079] [Table 16]

[0080] [Table 17]

Evaluation test Pesticide Dilution multiple release Spray after 6 days Spray 8 days after spray

 Number of living insects Number of eggs laid Number of living insects Number of eggs laid

2 3 1 Example C 2000+ 9 17 (1.9) 8 22 (2.8) Sun Crystal 600

 2 3 2 Example C 4000+ 10 28 (2.8) 6 38 (6.3) Sun Crystal 600

 2 3 3 Example C 8000+ 10 51 (5.1) 8 115 (14.4) Sun Crystal 600

 2 3 4 C C 2000 8 48 (6.0) 7 86 (12.3)

2 3 5 C C 4000 9 75 (8.3) 10 147 (14.7)

2 3 6 C C 8000 12 174 (14.5) 8 124 (15.5)

2 3 7 Comparative Example 1 Sun Crystal 600 13 175 (13.5) 12 186 (15.5)

2 3 8 No spray 15 227 (15.1) 14 220 (15.7)

[0081] C: colomite emulsion (1% milbemectin, manufactured by Sankyo Co., Ltd.)

 The first true leaves of the sprayed cucumber elongated 2.5-fold 8 days after spraying. After spraying, the effect of the colomite emulsion on the released adults of Nami-hamada was insignificant. The number of eggs laid by adults that migrated from the leaf surface to the back of the leaves decreased until 4 days after spraying with colomite emulsion alone, indicating an effect of suppressing egg production. By mixing the colomite emulsion and the sun crystal emulsion, the number of eggs laid further decreased, and the egg-laying inhibitory effect could be maintained until 8 days after application.

 [0082] Evaluation test 239—263

Twenty female adult Namihada (2 pots) were released on the primary leaves of pot-cultivated kidney beans (variety: Saki Midori No. 2). One day after the release, the insecticides shown in Table 18 were sprayed at a dilution factor (dilution with water) and concentration shown in Table 18 using a small atomizer. Adults and hatched larvae were examined ⁷ days after spraying, and the growth inhibition rate was calculated. Table 18 shows the results.

[0083] [Table 18] Evaluation Pesticide F Santa Rital Growth inhibition rate Test Dilution concentration Dilution concentration (%)

 Multiple (P m) Multiple (P P m)

 Ξ39 Example 2000 5.0 500 1800 100

 240 Example 1000 900 100

 241 Example 2000 450 100

 242 Example 4000 225 100

 243 Example 4000 2.5 500 1800 100

 244 Example 1000 900 100

 Ξ45 Example 2000 450 100

 246 Example 4000 225 100

 247 Example 8000 1.25 500 1S0 100

 248 Example 1000 900 100

 249 Example 2000 450 100

 250 Example 4000 225 97.5

251 Example 16000 0.625 500 1800 100

 Ξ52 Example 1000 900 98.6

253 Example 2000 450 97.5

Ξ54 Example 4000 225 92.3

255 Comparative Example 1 500 1800 49.1

256 Comparative Example 1 1000 900 47.7

257 Comparative Example 1 2000 450 32.0

Ξ58 Comparative Example 1 4000 225 10.9

259 F 2000 5.0 100

 260 F 4000 2.5 100

 261 F 8000 1.25 97.0

262 F 16000 0.625 65.0

263 No spraying 0

F: Afarm emulsion (manufactured by Syngenta Japan Co., Ltd., 1% emmamethine benzoate) Growth inhibition rate = (1-number of insects before treatment in untreated group X number of insects after treatment in treated group Z before treatment in treated group Z Number of insects X number of insects after treatment in untreated area) X 100

 Number of surviving untreated plots: 12 adults, 28 larvae, 40 in total

[0085] It was confirmed that even in the case of using afarm emulsion alone, the growth inhibitory effect was high, and in the 1.62 ppm section of afarm which is inferior to the force effect, a high effect was obtained by mixing the afarm emulsion and the sun crystal emulsion.

[0086] Evaluation test 264—288

Twenty female adult Namihada (2 pots) were released on the primary leaves of pot-cultivated kidney beans (variety: Saki Midori No. 2). One day after release, the insecticides shown in Table 19 were sprayed at the dilution factor (dilution with water) and concentration shown in Table 19 by a small atomizer. Adults and hatching ⁷ days after spraying The larvae were examined and the growth inhibition rate was calculated. The results are shown in Table 19.

[0087] [Table 19]

[0088] M: Megatop liquid (3.6% Nemadectin, manufactured by BS Efagro Co., Ltd.) Growth inhibition rate = (1-number of insects before treatment in untreated group X number of insects after treatment in treated group Z of treated group Number of insects before treatment X Number of insects after treatment in untreated area) X 100

[0089] In the case of using Megatop liquid alone, it was recognized that a stable effect was obtained by mixing the force megatop liquid with sun crystal, which had unstable effects.

[0090] Evaluation test 289—324

The insecticides shown in Table 20 were sprayed on the primary leaves of potato-cultivated kidney beans (variety: Sakimidori No. 2) at the dilution factor (dilution with water) and concentration shown in Table 20 using a small sprayer. After spraying, they were placed in a mini-tomato cultivated vine, which frequently occurs, and spawned and damaged. . Thirteen days after spraying, the number of larvae of the larva of Paramecium chinensis in the primary leaves was investigated, and the number of parasites was calculated to prevent damage. The results are shown in Table 20.

[0091] [Table 20]

[0092] F: Afarm emulsion (manufactured by Syngenta Japan Co., Ltd., 1% emmamethatin benzoate) Damage prevention rate = (1 number of parasites in the sprayed area Z number of parasites in the unsprayed area) x 100

[0093] In the case of afarm emulsion alone, a high effect was observed at 2000 times (5 ppm). Inferior effect The damage prevention effect was remarkably improved by mixing afarm emulsion and sun crystal in the concentration section of 2.5 ppm or less.

[0094] Evaluation test 325—334

 A pot aphid parasite was inoculated into a pot-grown cucumber (variety: Shinko A, 2 leaf stage). After examining the number of insects before spraying two days after inoculation, the insecticides shown in Table 21 were sprayed at a dilution factor shown in Table 21 (dilution with water) using a small sprayer. Three days after spraying, the number of parasites was examined, and a corrected density index was calculated. The results are shown in Table 21.

[0095] [Table 21]

[0096] C: Colomite emulsion (1% millbemectin, manufactured by Sankyo Co., Ltd.)

 F: Afarm emulsion (manufactured by Syngenta Japan Co., Ltd., 1% emamethatin benzoate) Corrected density index = number of pre-insects in untreated area X number of insects in treated area X number of pre-insects in treated area X none

 Insect count after treatment in treatment area

[0097] Although the use of the colomite emulsion or afarm emulsion alone was inferior in effect, the colomite emulsion or the afarm emulsion was mixed with a 900-fold diluted sun crystal. It was recognized that a high effect was obtained by doing so.

 [0098] Evaluation test 335—343

 The pesticides shown in Table 22 were sprayed on the new leaves of Chinokokorida infested by eggplants grown in the field (variety: Senryo No. 2) at the dilution factor shown in Table 22 (dilution with water) using a small sprayer. . Three days after spraying, the sprayed new leaves were cut off, and the number of surviving insects was examined under a microscope. The results are shown in Table 22.

 [0099] [Table 22]

[0100] C: colomite emulsion (1% millbemectin, manufactured by Sankyo Co., Ltd.)

 [0101] The use of colomite emulsion and Ardent wettable powder alone, which are ineffective, can provide a high effect by mixing the colomite emulsion with a 600-fold diluted sun crystal at 16000-fold and 32,000-fold. Admitted.

Claims

The scope of the claims  [1] An insecticide comprising fatty acid glyceride obtained from a fatty acid having 8 to 22 carbon atoms and glycerin, and a macrolide insecticide. [2] The insecticide according to [1], wherein the fatty acid forming the fatty acid glyceride is a plant-derived fatty acid.  3. The insecticide according to claim 1, wherein the concentration of the fatty acid glyceride is 200 to 3000 ppm, and the concentration of the macrolide insecticide is 0.2 to 100 ppm.  [4] The insecticide according to any one of [13] to [13], which is used for controlling pests of the second order, pests of the order Hemiptera, pests of the order Thysanoptera, or pests of the order Lepidoptera.