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WO2005010209A3 - Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren - Google Patents

Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren Download PDF

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Publication number
WO2005010209A3
WO2005010209A3 PCT/EP2004/008363 EP2004008363W WO2005010209A3 WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3 EP 2004008363 W EP2004008363 W EP 2004008363W WO 2005010209 A3 WO2005010209 A3 WO 2005010209A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
reverse transcription
amplification
pool
derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/008363
Other languages
English (en)
French (fr)
Other versions
WO2005010209A2 (de
Inventor
Tanja Wille
Christian Korfhage
Eric Lader
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Priority to CA002533119A priority Critical patent/CA2533119A1/en
Priority to JP2006520816A priority patent/JP2006528482A/ja
Priority to EP04763506A priority patent/EP1651776A2/de
Priority to US10/565,989 priority patent/US20060281092A1/en
Priority to AU2004259851A priority patent/AU2004259851A1/en
Publication of WO2005010209A2 publication Critical patent/WO2005010209A2/de
Publication of WO2005010209A3 publication Critical patent/WO2005010209A3/de
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Die vorliegende Erfindung betrifft ein Verfahren zur reversen Transkription und/oder Amplifikation eines Produktes aus einer reversen Transkription eines Pools von Nukleinsäuren eines bestimmten Typs, wobei dieser Pool von Nukleinsäuren aus einer komplexen biologischen Probe oder einer enzymatischen Reaktion stammt.
PCT/EP2004/008363 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren Ceased WO2005010209A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002533119A CA2533119A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
JP2006520816A JP2006528482A (ja) 2003-07-24 2004-07-26 核酸の逆転写及び／または増幅方法
EP04763506A EP1651776A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren
US10/565,989 US20060281092A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids
AU2004259851A AU2004259851A1 (en) 2003-07-24 2004-07-26 Method for the reverse transcription and/or amplification of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48964303P 2003-07-24 2003-07-24
US60/489,643 2003-07-24

Publications (2)

Publication Number Publication Date
WO2005010209A2 WO2005010209A2 (de) 2005-02-03
WO2005010209A3 true WO2005010209A3 (de) 2005-05-26

Family

ID=34102910

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/008363 Ceased WO2005010209A2 (de) 2003-07-24 2004-07-26 Verfahren zur reversen transkription und/oder amplifikation von nukleinsäuren

Country Status (6)

Country Link
US (1) US20060281092A1 (de)
EP (1) EP1651776A2 (de)
JP (1) JP2006528482A (de)
AU (1) AU2004259851A1 (de)
CA (1) CA2533119A1 (de)
WO (1) WO2005010209A2 (de)

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US7126215B2 (en) 2004-03-30 2006-10-24 Intel Corporation Microelectronic packages including nanocomposite dielectric build-up materials and nanocomposite solder resist
US20060234277A1 (en) * 2005-03-31 2006-10-19 Amgen Inc. Method for selectively blocking hemoglobin RNA amplification
BRPI0605857A (pt) * 2005-04-19 2007-12-18 Coding Tech Ab quantização dependente de energia para a codificação eficiente de parámetros de áudio espacial
EP2479291A1 (de) * 2005-10-27 2012-07-25 Life Technologies Corporation Nucleinsäureverstärkung unter Verwendung nichtzufälliger Primer
US10131935B2 (en) * 2006-07-11 2018-11-20 Aj Innuscreen Gmbh Method for parallel isolation of viral nucleic acids
WO2011032053A1 (en) * 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
EP2839024B1 (de) * 2012-04-16 2017-09-06 Life Technologies Corporation Verfahren zur herstellung von rna-bibliotheken
CN110964796B (zh) 2013-08-28 2024-04-05 贝克顿迪金森公司 大规模平行单细胞分析
EP3262192B1 (de) 2015-02-27 2020-09-16 Becton, Dickinson and Company Räumlich adressierbare molekulare barcodierung
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3303587A4 (de) * 2015-05-28 2018-11-21 Kaarel Krjutskov Blockierung von oligonukleotiden
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
CN113151423B (zh) 2016-08-05 2024-09-10 生物辐射实验室股份有限公司 第二链引导
KR102638006B1 (ko) 2016-09-26 2024-02-20 셀룰러 리서치, 인크. 바코딩된 올리고뉴클레오티드 서열을 갖는 시약을 이용한 단백질 발현의 측정
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
EP4345172A3 (de) 2017-06-05 2024-07-03 Becton, Dickinson and Company Probenindizierung für einzelzellen
WO2018232594A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 Pcr引物对及其应用
AU2019262048B2 (en) 2018-05-03 2025-09-04 Becton, Dickinson And Company High throughput multiomics sample analysis
EP4545647A3 (de) 2018-05-03 2025-07-09 Becton, Dickinson and Company Molekulare barcodierung auf entgegengesetzten transkriptenden
CN112771176A (zh) * 2018-09-25 2021-05-07 凯杰科技有限公司 耗竭不需要的rna物质
CN118853827A (zh) 2018-10-01 2024-10-29 贝克顿迪金森公司 确定5’转录物序列
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
EP3894552A1 (de) * 2018-12-13 2021-10-20 Becton, Dickinson and Company Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen
EP3914728B1 (de) 2019-01-23 2023-04-05 Becton, Dickinson and Company Mit antikörpern assoziierte oligonukleotide
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
EP4004231B1 (de) 2019-07-22 2025-11-12 Becton, Dickinson and Company Test für einzelzell-chromatin-immunpräzipitationssequenzierung
CN114729350A (zh) 2019-11-08 2022-07-08 贝克顿迪金森公司 使用随机引发获得用于免疫组库测序的全长v(d)j信息
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4097228B1 (de) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barkodierte wells zur räumlichen abbildung einzelner zellen durch sequenzierung
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (de) 2020-05-14 2023-03-22 Becton Dickinson and Company Primer zur profilierung von immunrepertoire
EP4407030B1 (de) 2020-06-02 2025-12-17 Becton, Dickinson and Company Oligonukleotide und perlen für 5 prime genexpressionsassay
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116194589A (zh) 2020-07-31 2023-05-30 贝克顿迪金森公司 用于转座酶可及染色质的单细胞测定
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2025054499A1 (en) * 2023-09-06 2025-03-13 North Carolina State University Compositions and methods relating to enzymatic dna synthesis

Citations (5)

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WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
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WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

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US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
CA2528109A1 (en) * 2003-06-06 2005-01-13 Gene Logic, Inc. Methods for enhancing gene expression analysis

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US5891625A (en) * 1992-06-05 1999-04-06 Buchardt Ole Use of nucleic acid analogues in the inhibition of nucleic acid amplification
WO2001032931A2 (en) * 1999-11-02 2001-05-10 Curagen Corporation Method and compositions for selectively inhibiting amplification of sequences in a population of nucleic acid molecules
WO2001060517A2 (de) * 2000-02-15 2001-08-23 Antigene Biotech Gmbh Gefäss zur nukleinsäureanalytik
WO2002000599A1 (de) * 2000-06-27 2002-01-03 Qiagen Gmbh Neue kompositionen für die isolierung und/oder stabilisierung von nukleinsäuren in biologischen materialien
CA2442092A1 (en) * 2001-03-26 2002-10-17 Ribozyme Pharmaceuticals, Inc. Oligonucleotide mediated inhibition of hepatitis b virus and hepatitis c virus replication

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Also Published As

Publication number Publication date
CA2533119A1 (en) 2005-02-03
JP2006528482A (ja) 2006-12-21
US20060281092A1 (en) 2006-12-14
AU2004259851A1 (en) 2005-02-03
EP1651776A2 (de) 2006-05-03
WO2005010209A2 (de) 2005-02-03

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