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WO2005059149A3 - Fabrication amelioree de proteines - Google Patents

Fabrication amelioree de proteines Download PDF

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Publication number
WO2005059149A3
WO2005059149A3 PCT/EP2004/053378 EP2004053378W WO2005059149A3 WO 2005059149 A3 WO2005059149 A3 WO 2005059149A3 EP 2004053378 W EP2004053378 W EP 2004053378W WO 2005059149 A3 WO2005059149 A3 WO 2005059149A3
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WO
WIPO (PCT)
Prior art keywords
expression
opener
sequence
unit comprises
transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/EP2004/053378
Other languages
English (en)
Other versions
WO2005059149A2 (fr
Inventor
Arie Pieter Otte
Theodorus Hendrikus Jaco Kwaks
Richard George Antonius Sewalt
Blokland Henricus Johannes Van
Abraham Bout
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Janssen Vaccines and Prevention BV
Chromagenics BV
Original Assignee
Chromagenics BV
Crucell Holand BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics BV, Crucell Holand BV filed Critical Chromagenics BV
Publication of WO2005059149A2 publication Critical patent/WO2005059149A2/fr
Publication of WO2005059149A3 publication Critical patent/WO2005059149A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des cellules de rétine E1 immortalisées comprenant une unité d'expression de protéines recombinantes, ladite unité comprenant un promoteur lié de façon fonctionnelle à un cadre de lecture ouvert codant pour au moins une protéine d'intérêt et caractérisé en ce que ladite unité d'expression comprend au moins un élément améliorant l'expression, ledit élément améliorant l'expression étant choisi dans le groupe constitué de a) une séquence stabilisée anti-répresseurs (STAR); b) une séquence d'interruption de transcription (TRAP); et c) un site de liaison destiné à un élément d'un système de modification de chromatine destiné à rendre la chromatine plus accessible à la transcription (système d'ouverture), ledit système d'ouverture étant présent dans ladite cellule. L'invention concerne également des procédés de la fabrication d'au moins une desdites protéines recombinantes dans ces cellules.
PCT/EP2004/053378 2003-12-12 2004-12-09 Fabrication amelioree de proteines Ceased WO2005059149A2 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP0350999 2003-12-12
EPPCT/EP03/50999 2003-12-12
EP0351038 2003-12-17
EPPCT/EP03/51038 2003-12-17

Publications (2)

Publication Number Publication Date
WO2005059149A2 WO2005059149A2 (fr) 2005-06-30
WO2005059149A3 true WO2005059149A3 (fr) 2006-05-26

Family

ID=34702159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/053378 Ceased WO2005059149A2 (fr) 2003-12-12 2004-12-09 Fabrication amelioree de proteines

Country Status (1)

Country Link
WO (1) WO2005059149A2 (fr)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2123772A1 (fr) 2008-04-29 2009-11-25 DSM IP Assets B.V. Souches produisant des antibiotiques bêta-lactamines
WO2010015627A2 (fr) 2008-08-05 2010-02-11 Dsm Ip Assets B.V. Souches productrices d'adipoyl-7-adca
EA023558B1 (ru) 2009-04-24 2016-06-30 ДСМ АйПи АССЕТС Б.В. ТЕРМОСТАБИЛЬНЫЙ ПОЛИПЕПТИД С АКТИВНОСТЬЮ β-ЦЕЛЛОБИОГИДРОЛАЗЫ И ЕГО ПРИМЕНЕНИЕ ДЛЯ ПРОИЗВОДСТВА САХАРОВ ИЗ ЛИГНОЦЕЛЛЮЛОЗНОГО МАТЕРИАЛА
MX2012009282A (es) 2010-02-11 2012-09-07 Dsm Ip Assets Bv Polipeptido que tiene actividad de celobiohidrolasa y usos del mismo.
EA201300058A1 (ru) 2010-06-29 2013-06-28 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий бета-глюкозидазной активностью, и его применение
MX2012014993A (es) 2010-06-29 2013-02-26 Dsm Ip Assets Bv Polipeptido que tiene actividad degradante de carbohidratos y usos de los mismos.
AU2011273686B2 (en) 2010-06-29 2014-07-10 Versalis S.P.A. Polypeptide having acetyl xylan esterase activity and uses thereof
EP2588603B1 (fr) 2010-06-29 2017-03-22 DSM IP Assets B.V. Polypeptide améliorant la cellulase et utilisations correspondantes
EA201300054A1 (ru) 2010-06-29 2013-05-30 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий активностью бета-глюкозидазы, и его применение
BR112012033396A2 (pt) 2010-06-29 2015-06-23 Dsm Ip Assets Bv Polipetídeo tendo atividade swollenin e seus usos.
PL3447136T3 (pl) 2013-02-04 2020-10-19 Dsm Ip Assets B.V. Polipeptyd degradujący węglowodany i jego zastosowania
WO2014202620A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
WO2014202622A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
WO2014202624A2 (fr) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Gène de rasamsonia et son utilisation
WO2014202621A1 (fr) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Polypeptide de dégradation des hydrates de carbone et utilisations de ce dernier
EP3469076B1 (fr) 2016-06-10 2020-09-02 DSM IP Assets B.V. Lipase mutante et son utilisation
KR20210013097A (ko) 2018-05-18 2021-02-03 디에스엠 아이피 어셋츠 비.브이. 돌연변이체 리파아제 및 이의 용도
CA3100611A1 (fr) 2018-05-18 2019-11-21 Dsm Ip Assets B.V. Lipase mutante et utilisation associee

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063403A2 (fr) * 1999-04-15 2000-10-26 Crucell Holland B.V. Production de proteine de recombinaison dans une cellule humaine
WO2003004704A2 (fr) * 2001-07-04 2003-01-16 Chromagenics B.V. Sequences d'adn comprenant des qualites de regulation de la transcription genique et methodes de detection et d'utilisation de ces sequences d'adn

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000063403A2 (fr) * 1999-04-15 2000-10-26 Crucell Holland B.V. Production de proteine de recombinaison dans une cellule humaine
WO2003004704A2 (fr) * 2001-07-04 2003-01-16 Chromagenics B.V. Sequences d'adn comprenant des qualites de regulation de la transcription genique et methodes de detection et d'utilisation de ces sequences d'adn

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
PFARR D S ET AL: "DIFFERENTIAL EFFECTS OF POLYADENYLATION REGIONS ON GENE EXPRESSION IN MAMMALIAN CELLS", DNA, MARY ANN LIEBERT, NEW YORK, NY, US, vol. 5, no. 2, 1986, pages 115 - 122, XP002910674, ISSN: 1044-5498 *

Also Published As

Publication number Publication date
WO2005059149A2 (fr) 2005-06-30

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