AU2001247842A1 - A promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producingfungus rhizopus oryzae and a method of expressing a gene of interest in fungal species - Google Patents
A promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producingfungus rhizopus oryzae and a method of expressing a gene of interest in fungal speciesInfo
- Publication number
- AU2001247842A1 AU2001247842A1 AU2001247842A AU4784201A AU2001247842A1 AU 2001247842 A1 AU2001247842 A1 AU 2001247842A1 AU 2001247842 A AU2001247842 A AU 2001247842A AU 4784201 A AU4784201 A AU 4784201A AU 2001247842 A1 AU2001247842 A1 AU 2001247842A1
- Authority
- AU
- Australia
- Prior art keywords
- gene
- polynucleotide
- phosphoglycerate kinase
- promoter sequence
- expressing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000894007 species Species 0.000 title abstract 5
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 title abstract 3
- 108091000080 Phosphotransferase Proteins 0.000 title abstract 3
- 230000002538 fungal effect Effects 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 240000005384 Rhizopus oryzae Species 0.000 title abstract 2
- 235000013752 Rhizopus oryzae Nutrition 0.000 title abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 9
- 108091033319 polynucleotide Proteins 0.000 abstract 9
- 239000002157 polynucleotide Substances 0.000 abstract 9
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 101150079312 pgk1 gene Proteins 0.000 abstract 2
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 abstract 1
- 101710139464 Phosphoglycerate kinase 1 Proteins 0.000 abstract 1
- 102000020233 phosphotransferase Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1217—Phosphotransferases with a carboxyl group as acceptor (2.7.2)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
The invention includes polynucleotide constructs that contain a polynucleotide promoter sequence from the phosphoglycerate kinase 1 (pgk1) gene from Rhizopus oryzae. The invention also includes methods of expressing a polypeptide in a host cell using a polynucleotide construct containing a 3-phosphoglycerate kinase (pgk) gene promoter sequence operably linked to a polynucleotide encoding a polypeptide of interest. The invention also includes a system for producing a polypeptide. The system has a first polynucleotide sequence containing a 3-phosphoglycerate kinase gene promoter sequence isolated from a fungal species, and a second polynucleotide sequence which has been isolated from a second species and which encodes a polypeptide. A host cell contains the first polynucleotide sequence operably linked to the second polynucleotide sequence. The host cell is from a different species than the fungal species from which the first polynucleotide sequence is isolated.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/536,034 US6465635B2 (en) | 2000-03-27 | 2000-03-27 | Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species |
| US09536034 | 2000-03-27 | ||
| PCT/US2001/009913 WO2001073083A2 (en) | 2000-03-27 | 2001-03-27 | Promoter sequence of 3-phosphoglycerate kinase gene 1 of rhizopus oryzae and its use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2001247842A1 true AU2001247842A1 (en) | 2001-10-08 |
Family
ID=24136857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2001247842A Abandoned AU2001247842A1 (en) | 2000-03-27 | 2001-03-27 | A promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producingfungus rhizopus oryzae and a method of expressing a gene of interest in fungal species |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US6465635B2 (en) |
| EP (1) | EP1268824B1 (en) |
| AT (1) | ATE288495T1 (en) |
| AU (1) | AU2001247842A1 (en) |
| DE (1) | DE60108729T2 (en) |
| WO (1) | WO2001073083A2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6900305B2 (en) * | 2000-08-04 | 2005-05-31 | Battelle Memorial Institute | Isolated yeast promoter sequence and a method of regulated heterologous expression |
| JP6276479B2 (en) * | 2015-08-06 | 2018-02-07 | 花王株式会社 | New promoter |
| WO2017135317A1 (en) | 2016-02-04 | 2017-08-10 | 花王株式会社 | Method for producing mutant filamentous fungi |
| US10947522B2 (en) | 2016-02-04 | 2021-03-16 | Kao Corporation | Mutant of genus Rhizopus |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03247286A (en) * | 1990-02-26 | 1991-11-05 | Nippon Shokuhin Kako Co Ltd | Promoter gene of phosphoglyceric acid kinase gene originated from rhizopus genus |
| JP3247286B2 (en) | 1995-12-18 | 2002-01-15 | 帝人株式会社 | Cellulose acetate multifilament yarn having a special cross section and method for producing the same |
-
2000
- 2000-03-27 US US09/536,034 patent/US6465635B2/en not_active Expired - Fee Related
-
2001
- 2001-03-27 EP EP01920829A patent/EP1268824B1/en not_active Expired - Lifetime
- 2001-03-27 DE DE60108729T patent/DE60108729T2/en not_active Expired - Fee Related
- 2001-03-27 AU AU2001247842A patent/AU2001247842A1/en not_active Abandoned
- 2001-03-27 AT AT01920829T patent/ATE288495T1/en not_active IP Right Cessation
- 2001-03-27 WO PCT/US2001/009913 patent/WO2001073083A2/en not_active Ceased
-
2002
- 2002-08-07 US US10/214,655 patent/US20030045701A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20030045701A1 (en) | 2003-03-06 |
| DE60108729T2 (en) | 2006-05-11 |
| WO2001073083B1 (en) | 2002-05-16 |
| US6465635B2 (en) | 2002-10-15 |
| EP1268824B1 (en) | 2005-02-02 |
| DE60108729D1 (en) | 2005-03-10 |
| ATE288495T1 (en) | 2005-02-15 |
| US20020102636A1 (en) | 2002-08-01 |
| WO2001073083A2 (en) | 2001-10-04 |
| WO2001073083A3 (en) | 2002-02-28 |
| EP1268824A2 (en) | 2003-01-02 |
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