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WO2004017728A3 - Procedes - Google Patents

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Publication number
WO2004017728A3
WO2004017728A3 PCT/GB2003/003647 GB0303647W WO2004017728A3 WO 2004017728 A3 WO2004017728 A3 WO 2004017728A3 GB 0303647 W GB0303647 W GB 0303647W WO 2004017728 A3 WO2004017728 A3 WO 2004017728A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
relates
insect
cyp
insecticidal agents
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB2003/003647
Other languages
English (en)
Other versions
WO2004017728A2 (fr
Inventor
Sheetal Mansukhlal Shah
Robert John Lind
John David Windass
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syngenta Ltd
Original Assignee
Syngenta Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Ltd filed Critical Syngenta Ltd
Priority to AU2003264722A priority Critical patent/AU2003264722A1/en
Publication of WO2004017728A2 publication Critical patent/WO2004017728A2/fr
Publication of WO2004017728A3 publication Critical patent/WO2004017728A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • A01K67/65—Genetically modified arthropods
    • A01K67/68—Genetically modified insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A01K2217/058—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function due to expression of inhibitory nucleic acid, e.g. siRNA, antisense
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

L'invention concerne un procédé de criblage d'agents insecticides potentiels au moyen de gènes codant pour des enzymes P450 d'insectes. L'invention porte notamment sur des procédés d'utilisation de gènes cyp d'insectes et/ou de leurs promoteurs pour l'identification et/ou l'optimisation d'agents insecticides potentiels. Lesdits procédés utilisent des cellules transgéniques et/ou des organismes entiers dans lesquels au moins un gène cyp est délété ou sous-exprimé.
PCT/GB2003/003647 2002-08-23 2003-08-19 Procedes Ceased WO2004017728A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003264722A AU2003264722A1 (en) 2002-08-23 2003-08-19 Methods

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0219723.4 2002-08-23
GBGB0219723.4A GB0219723D0 (en) 2002-08-23 2002-08-23 Methods

Publications (2)

Publication Number Publication Date
WO2004017728A2 WO2004017728A2 (fr) 2004-03-04
WO2004017728A3 true WO2004017728A3 (fr) 2004-12-09

Family

ID=9942906

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/003647 Ceased WO2004017728A2 (fr) 2002-08-23 2003-08-19 Procedes

Country Status (3)

Country Link
AU (1) AU2003264722A1 (fr)
GB (1) GB0219723D0 (fr)
WO (1) WO2004017728A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109112117B (zh) * 2017-09-04 2020-11-27 华中农业大学 一种分离的二化螟cyp15c1基因及其编码蛋白

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008083598A1 (fr) * 2006-12-30 2008-07-17 Zhicheng Shen Culture transgénique sélectivement éliminée, préparation et utilisation correspondantes
CN104059931B (zh) * 2014-07-03 2017-01-11 西南大学柑桔研究所 桔小实蝇细胞色素p450基因的rna干扰载体及其构建方法和应用
CN118895286A (zh) * 2024-08-09 2024-11-05 扬州大学 一种褐飞虱细胞色素P450编码基因NlCYP303A1及其应用
CN119220549A (zh) * 2024-09-26 2024-12-31 华中农业大学 褐飞虱基因NlCYP303A1及其纳米核酸农药

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040470A2 (fr) * 1997-03-07 1998-09-17 Novartis Ag Cytochrome p450 monooxygenases
WO2001092561A2 (fr) * 2000-06-02 2001-12-06 Purdue Research Foundation Procede de criblage de resistance croisee negative
WO2003025223A2 (fr) * 2001-09-20 2003-03-27 The University Of Bath Ameliorations apportees au criblage d'insecticides ou s'y rapportant

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998040470A2 (fr) * 1997-03-07 1998-09-17 Novartis Ag Cytochrome p450 monooxygenases
WO2001092561A2 (fr) * 2000-06-02 2001-12-06 Purdue Research Foundation Procede de criblage de resistance croisee negative
WO2003025223A2 (fr) * 2001-09-20 2003-03-27 The University Of Bath Ameliorations apportees au criblage d'insecticides ou s'y rapportant

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DABORN P ET AL: "DDT resistance in Drosophila correlates with Cyp6g1 over-expression and confers cross-resistance to the neonicotinoid imidacloprid", MGG MOLECULAR GENETICS AND GENOMICS, vol. 266, no. 4, December 2001 (2001-12-01), &, pages 556 - 563, XP002277771, ISSN: 1617-4615 *
DUNKOV B C ET AL: "THE DROSOPHILA CYTOCHROME P450 GENE CYP6A2: STRUCTURE, LOCALIZATION, HETEROLOGOUS EXPRESSION, AND INDUCTION BY PHENOBARBITAL", DNA, MARY ANN LIEBERT, NEW YORK, NY, US, vol. 16, no. 11, November 1997 (1997-11-01), pages 1345 - 1356, XP008020802, ISSN: 1044-5498 *
TIJET NATHALIE ET AL: "The cytochrome P450 gene superfamily in Drosophila melanogaster: Annotation, intron-exon organization and phylogeny", GENE (AMSTERDAM), vol. 262, no. 1-2, 10 January 2001 (2001-01-10), &, pages 189 - 198, XP002277772, ISSN: 0378-1119 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109112117B (zh) * 2017-09-04 2020-11-27 华中农业大学 一种分离的二化螟cyp15c1基因及其编码蛋白

Also Published As

Publication number Publication date
AU2003264722A1 (en) 2004-03-11
AU2003264722A8 (en) 2004-03-11
GB0219723D0 (en) 2002-10-02
WO2004017728A2 (fr) 2004-03-04

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