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WO2004010780A3 - Innocuous intracellular ice - Google Patents

Innocuous intracellular ice Download PDF

Info

Publication number
WO2004010780A3
WO2004010780A3 PCT/US2003/024147 US0324147W WO2004010780A3 WO 2004010780 A3 WO2004010780 A3 WO 2004010780A3 US 0324147 W US0324147 W US 0324147W WO 2004010780 A3 WO2004010780 A3 WO 2004010780A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
intracellular ice
innocuous
cooling
ice formation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2003/024147
Other languages
French (fr)
Other versions
WO2004010780A2 (en
Inventor
Jason Paul Acker
Locksley Earl Mcgann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Biotechnology LLC
Original Assignee
General Biotechnology LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Biotechnology LLC filed Critical General Biotechnology LLC
Priority to AU2003258001A priority Critical patent/AU2003258001A1/en
Publication of WO2004010780A2 publication Critical patent/WO2004010780A2/en
Publication of WO2004010780A3 publication Critical patent/WO2004010780A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/16—Physical preservation processes
    • A01N1/162—Temperature processes, e.g. following predefined temperature changes over time
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a method for cryopreserving cells and maintaining cell viability. The method comprises the steps of predetermining the nucleation temperature for extracellular and intracellular ice formation for the cells, cooling the cells to the predetermined nucleation temperature, nucleating intracellular ice formation, and cooling the cells to a temperature lower than the predetermined nucleation temperature. In another embodiment a method is provided for cryopreserving cells and maintaining cell viability without the use of cryoprotective compounds. The method comprises the step of cooling the cells under conditions to optimize controlled innocuous intracellular ice formation.
PCT/US2003/024147 2002-07-30 2003-07-30 Innocuous intracellular ice Ceased WO2004010780A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003258001A AU2003258001A1 (en) 2002-07-30 2003-07-30 Innocuous intracellular ice

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39995302P 2002-07-30 2002-07-30
US60/399,953 2002-07-30

Publications (2)

Publication Number Publication Date
WO2004010780A2 WO2004010780A2 (en) 2004-02-05
WO2004010780A3 true WO2004010780A3 (en) 2004-05-13

Family

ID=31188648

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/024147 Ceased WO2004010780A2 (en) 2002-07-30 2003-07-30 Innocuous intracellular ice

Country Status (2)

Country Link
AU (1) AU2003258001A1 (en)
WO (1) WO2004010780A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005250053B2 (en) * 2004-06-02 2009-08-06 Es Cell International Pte Ltd Cell preservation method
CA2569485C (en) * 2004-06-02 2015-08-18 Es Cell International Pte Ltd. Cell preservation method
US8794013B2 (en) 2006-02-10 2014-08-05 Praxair Technology, Inc. Method and system for nucleation control in a controlled rate freezer (CRF)
CN113615681A (en) * 2021-08-27 2021-11-09 郑州源创吉因实业有限公司 Frozen stock solution and frozen stock method for immune cells

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3842831A (en) * 1972-10-27 1974-10-22 Genetic Labor Inc Cellular skin patch
US5689961A (en) * 1996-01-30 1997-11-25 Organogenesis Inc. Ice seeding apparatus for cryopreservation systems
US5891617A (en) * 1993-09-15 1999-04-06 Organogenesis Inc. Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
US5964096A (en) * 1996-01-30 1999-10-12 Organogenesis Inc. Method and package design for cryopreservation and storage of cultured tissue equivalents
US6140123A (en) * 1998-10-07 2000-10-31 Cedars-Sinai Medical Center Method for conditioning and cryopreserving cells

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3842831A (en) * 1972-10-27 1974-10-22 Genetic Labor Inc Cellular skin patch
US5891617A (en) * 1993-09-15 1999-04-06 Organogenesis Inc. Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
US5689961A (en) * 1996-01-30 1997-11-25 Organogenesis Inc. Ice seeding apparatus for cryopreservation systems
US5964096A (en) * 1996-01-30 1999-10-12 Organogenesis Inc. Method and package design for cryopreservation and storage of cultured tissue equivalents
US6140123A (en) * 1998-10-07 2000-10-31 Cedars-Sinai Medical Center Method for conditioning and cryopreserving cells

Also Published As

Publication number Publication date
WO2004010780A2 (en) 2004-02-05
AU2003258001A8 (en) 2004-02-16
AU2003258001A1 (en) 2004-02-16

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