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WO2004099428A1 - Procede d'extraction de beta glucane a partir de phellinus linteus par la destruction cellulaire et procede de fermentation d'enzymes - Google Patents

Procede d'extraction de beta glucane a partir de phellinus linteus par la destruction cellulaire et procede de fermentation d'enzymes Download PDF

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Publication number
WO2004099428A1
WO2004099428A1 PCT/KR2004/001055 KR2004001055W WO2004099428A1 WO 2004099428 A1 WO2004099428 A1 WO 2004099428A1 KR 2004001055 W KR2004001055 W KR 2004001055W WO 2004099428 A1 WO2004099428 A1 WO 2004099428A1
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WIPO (PCT)
Prior art keywords
phellinus linteus
glucan
extraction
mixture
phellinus
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Ceased
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PCT/KR2004/001055
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English (en)
Inventor
Kwan-Ho Kim
Il-Geun Roh
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • E—FIXED CONSTRUCTIONS
    • E06—DOORS, WINDOWS, SHUTTERS, OR ROLLER BLINDS IN GENERAL; LADDERS
    • E06B—FIXED OR MOVABLE CLOSURES FOR OPENINGS IN BUILDINGS, VEHICLES, FENCES OR LIKE ENCLOSURES IN GENERAL, e.g. DOORS, WINDOWS, BLINDS, GATES
    • E06B9/00—Screening or protective devices for wall or similar openings, with or without operating or securing mechanisms; Closures of similar construction
    • E06B9/24—Screens or other constructions affording protection against light, especially against sunshine; Similar screens for privacy or appearance; Slat blinds
    • E06B9/26—Lamellar or like blinds, e.g. venetian blinds
    • E06B9/36—Lamellar or like blinds, e.g. venetian blinds with vertical lamellae ; Supporting rails therefor
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
    • E—FIXED CONSTRUCTIONS
    • E06—DOORS, WINDOWS, SHUTTERS, OR ROLLER BLINDS IN GENERAL; LADDERS
    • E06B—FIXED OR MOVABLE CLOSURES FOR OPENINGS IN BUILDINGS, VEHICLES, FENCES OR LIKE ENCLOSURES IN GENERAL, e.g. DOORS, WINDOWS, BLINDS, GATES
    • E06B9/00—Screening or protective devices for wall or similar openings, with or without operating or securing mechanisms; Closures of similar construction
    • E06B9/24—Screens or other constructions affording protection against light, especially against sunshine; Similar screens for privacy or appearance; Slat blinds
    • E06B9/26—Lamellar or like blinds, e.g. venetian blinds
    • E06B9/38—Other details
    • E06B9/386—Details of lamellae

Definitions

  • the present invention relates to a method for efficiently extracting and isolating ⁇ -glucan from Phellinus linteus in high yield.
  • Phellinus species are very rare perennial mushrooms in nature which grow on mulberry and broadleaved trees in the high reaches, and several tens of species have been identified to date.
  • Representative Phellinus spp. are Phellinus linteus and Phellinus baumi which mainly grow on the trunk of mulberry and broadleaved trees. According to a recent report, Phellinus linteus which has been allowed only as a medical raw material can be used as a raw material for foods (see, an article from the Korean daily economic newspaper dated February 7, 2003).
  • Phellinus linteus contains a large quantity of nutrients beneficial to humans, such as potassium, calcium, magnesium, vitamin B2, B3 and C, fibroid materials, amino acids and the like, and in particular, ⁇ - glucan, which is a polysaccharide substance.
  • ⁇ -glucan is a medicinal and therapeutically active ingredient.
  • the fact that ⁇ -glucan has immunopotentiating effects and anticancer effects is found in many related literatures and Korean traditional herb medical books, including Dongeuibogam, Sinnongbonchogyeong and Hyangyakjipsungbang.
  • Phellinus linteus has inhibitory effects against viruses, bacteria, fungi and parasites, inhibitory effects against tumors (cancers) at an inhibition level of 96.7%, and antioxidant effects against oxygen radicals and free radicals through immunopotentiating mechanisms.
  • ⁇ -glucan contained in Phellinus linteus is present in cell walls in a protein-bound state, and is surrounded by a large amount of water-insoluble fibroid materials.
  • Phellinus linteus includes fruiting bodies composed of a number of hard and thick cell layers. Korean Patent Laid-open No.
  • 2003-30636 discloses a method for producing a protein-polysaccharide by adding an organic substance and an organic nutrient to the fruiting bodies of a mushroom to obtain a fermentation mixture, inoculating the mixture with the mycelia of the ⁇ basidiomycetes or yeast, followed by fermentation, hot-water extraction, dialysis, centrifugation and lyophilization.
  • the texture of the fruiting bodies is softened by fermentation so that the extraction of the protein-polysaccharide is facilitated and thus the protein-polysaccharide is produced in high yield.
  • other fermentation metabolites as well as ingredients inherent to the mushroom are generated, it is difficult to collect functional ingredients of the mushroom in the natural state.
  • the hard fruiting bodies are finely pulverized, they are too large in size to be used as substrates of an enzyme generated during fermentation by the inoculated mycelia of the basidiomycetes or yeast.
  • the fruiting bodies are highly insoluble, it is almost impossible to collect ⁇ -glucan present in the cell walls on a large scale.
  • the fruiting bodies contain impurities other than ⁇ -glucan.
  • Korean Patent No. 308392 discloses a method for producing an extract of natural Phellinus linteus by mixing pulverized - Phellinus linteus with a solvent, and boiling the mixture in a heating bath under reduced pressure. At this time, the mixture is subjected to super high frequency sonication for 24 hours in order to increase the extraction efficiency.
  • This method requires a long time for extraction, and uses an organic solvent such as 1,3-butylene chloride, propyleneglycol or isopropanol as an extracting solvent. Accordingly, the method is improper to separate highly polar ⁇ -glucan present in the cell walls on a large scale and to extract inherent ingredients of Phellinus linteus.
  • an organic solvent such as 1,3-butylene chloride, propyleneglycol or isopropanol
  • Phellinus linteus is an expensive oriental herb costing as much as hundreds or thousands of U.S. dollars per kg
  • the hot- water extraction must be reconsidered because it is uneconomical.
  • the medicinal ⁇ -glucan is present in hard cell layers and is a highly water-insoluble macromolecule having a molecular weight on the order of 1,000,000 daltons. Since the quantification of the macromolecular ⁇ - • glucan has not been sufficiently investigated, the simple hot- water extraction must be reconsidered under this situation.
  • Figs. 1 to 5 are optical microscope images (1250x) showing changes in the morphology of the fruiting bodies of Phellinus linteus powders and residues obtained after extraction and centrifugation of the Phellinus linteus powders, which are obtained in Example 1 and Comparative Examples 1 and 2 of the present invention.
  • Fig. 1 is an optical microscope image of Phelinus Linteus
  • Fig. 2 is an optical microscope image of pulverized Phellinus linteus
  • Fig. 3 is an optical microscope image of ultrasonically pulverized Phellinus linteus
  • Fig. 4 is an optical microscope image of hot-water treated Phellinus linteus under high pressure
  • Fig. 5 is an optical microscope image of enzyme-treated Phellinus linteus.
  • the present invention has been made in view of the above problems, and it is an object of the present invention to provide a method for efficiently extracting and isolating ⁇ -glucan and other medically effective ingredients from Phellinus linteus.
  • the Phellinus linteus is subjected to sonication and the hard cell layers are degraded under high pressure hot-water extraction conditions, thereby forming a condition under which protease and cellulase can function, hi addition, since the content of soluble ingredients is determined by a dry process, the total extraction efficiency can be measured.
  • the extraction efficiency of ⁇ -glucan can be quantitatively determined by an ultrafiltration method using the molecular size of ⁇ -glucan.
  • a method for extracting medically effective ingredients from Phellinus linteus comprising the steps of: adding a pulverized Phellinus linteus powder into a distilled water of an amount ranging from 20 to 50 times based on the weight of the powder to make a mixture, and ultrasonicating the mixture; extracting the mixture with hot- water under a pressure of 2 to 3 atm, and filtering the extract through a membrane filter to obtain a filtrate; and enzyme-reacting the filtrate with cellulase and alkalase and subjecting to ultrafiltration on a membrane filter.
  • Phellinus linteus according to the present invention is very economical and quantitative. According to the method of the present invention, medically effective ingredients of expensive Phellinus linteus can be utilized in the various industrial fields such as pharmaceuticals, cosmetics and foods in an economic and efficient manner.
  • Comparative Example 1 Hot- water extraction of samples and ultrafiltration lOg of each of Phellinus linteus powder was extracted with about 300ml of distilled water at 100°C for 1, 2, 3 and 4 hours, followed by centrifugation. The resulting supernatant was concentrated with heating to a volume of 15ml. 5ml of the concentrate was separately dried to obtain a dry extract. The dry extract was weighed, and the result was multiplied by three. The obtained value was used as an extraction rate of the total dry extract. The results are summarized in Table 1 below. The remaining 10ml of the concentrate was subjected to ultrafiltration on a membrane filter having a pore size of 7,000 MWCO (Molecular Weight Cut Off).
  • Comparative Example 2 Hot- water extraction of samples under high pressure and ultrafiltration
  • Example 1 Ultrasonic hot-water extraction of samples under high pressure and ultrafiltration
  • Example 1 Determination of content of crude ⁇ -glucan in dry extract prepared in Example 1
  • the content of crude ⁇ -glucan in the dry extracts prepared in Example 1 was determined in accordance with methods of Mannors, D. J. (1973, The structure of a ⁇ -(l,6)-D-glucan, from yeast cell walls. Biochem. J. 135, 19-30) and Catley, B. J. (1988. Isolation and analysis of cell walls Yeast (A practical approach). IRL press, pp. 163-183).
  • the content of crude ⁇ -glucan in the dry extracts was determined by measuring the glucose content in glucan fractions obtained through chemical treatment using a NaOH solution.
  • Figs. 1 to 5 are optical microscope images (1250x) showing changes in the morphology of the fruiting bodies of the Phellinus linteus powders and the residues obtained after extraction and centrifugation of the Phellinus linteus powders, which were obtained in Example 1 and Comparative Examples 1 and 2.
  • Fig. 1 is an optical microscope image of Phelinus Linteus
  • Fig. 2 is an optical microscope image of the pulverized Phellinus linteus
  • Fig. 3 is an optical microscope image of the ultrasonically pulverized Phellinus linteus
  • Fig. 4 is an optical microscope image of the hot-water treated Phellinus linteus under high pressure
  • Fig. 5 is an optical microscope image of the enzyme-treated Phellinus linteus.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Structural Engineering (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Architecture (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Civil Engineering (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

L'invention concerne un procédé d'extraction et d'isolement de β-glucane brut en tant qu'ingrédient médicalement efficace à partir de Phellinus linteus à haut rendement. Plus précisément, ce procédé permet d'augmenter la vitesse d'extraction de β-glucane brut à partir de Phellinus linteus au moyen de la dégradation des organes de fructification de Phellinus linteus par sonication, destruction de parois cellulaires au moyen d'eau chaude soumise à une pression élevée. Il permet aussi d'augmenter la fermentation d'enzymes.
PCT/KR2004/001055 2003-05-07 2004-05-06 Procede d'extraction de beta glucane a partir de phellinus linteus par la destruction cellulaire et procede de fermentation d'enzymes Ceased WO2004099428A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020030028783A KR20040096000A (ko) 2003-05-07 2003-05-07 초음파에 의한 세포벽 파쇄 및 효소발효법을 이용한상황버섯 베타글루칸 추출방법
KR10-2003-0028783 2003-05-07

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PCT/KR2003/001723 Ceased WO2004099427A1 (fr) 2003-05-07 2003-08-26 Procede d'extraction de beta-glucane a partir de phellinus linteus
PCT/KR2004/001055 Ceased WO2004099428A1 (fr) 2003-05-07 2004-05-06 Procede d'extraction de beta glucane a partir de phellinus linteus par la destruction cellulaire et procede de fermentation d'enzymes

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104592409A (zh) * 2015-01-12 2015-05-06 陕西师范大学 一种低压低温提取高活性桑黄多糖的方法
CN106749734A (zh) * 2016-12-26 2017-05-31 庆阳敦博科技发展有限公司 甜百合多糖提取方法
KR101879506B1 (ko) * 2016-10-05 2018-07-17 경북대학교 산학협력단 상황버섯으로부터 베타글루칸의 추출방법
US10323263B2 (en) 2015-02-03 2019-06-18 Tate & Lyle Sweden Ab Methods for producing liquid compositions comprising β-glucan

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KR100825396B1 (ko) * 2007-02-28 2008-04-29 주식회사 한불후치피아 동식물성 유기조직체로부터 수용성 액체 성분을 추출하는방법
KR100908034B1 (ko) * 2007-06-18 2009-07-20 계명대학교 산학협력단 상황버섯 추출물, 이를 포함하는 조청 및 고추장
KR100981347B1 (ko) * 2007-11-23 2010-09-10 강원대학교산학협력단 복분자 추출방법
KR101477140B1 (ko) * 2012-11-13 2014-12-29 한국식품연구원 β-글루칸 제조방법
KR101687985B1 (ko) 2015-08-27 2016-12-23 주식회사 엔에스텍 아임계추출법을 이용한 버섯의 베타 글루칸 추출방법
CN109369818A (zh) * 2018-10-12 2019-02-22 齐鲁工业大学 一种超声波协同蒸煮法提取桉木木聚糖的方法
CN111635916A (zh) * 2020-06-28 2020-09-08 上海应用技术大学 一种用于酵母β-葡聚糖提取的预处理方法

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JPH11318432A (ja) * 1998-04-30 1999-11-24 Korea Advanced Inst Of Sci Technol 新規のペリヌス属菌株から分離した免疫増強活性多糖類物質及びその使用
KR100303886B1 (ko) * 1994-11-19 2001-11-22 임곤혁 상황버섯으로부터항암성분을추출하는방법
JP2002171936A (ja) * 2000-12-05 2002-06-18 Yukito Akiyama 健康食品及びその製造方法
KR20030072417A (ko) * 2002-03-04 2003-09-15 박홍제 버섯 균사체와 균사배양액 유래의 기능성 다당류의 추출법및 추출물을 이용한 기능성 차 또는/및 식품의 제조방법

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JP2002000229A (ja) * 2000-06-23 2002-01-08 S F C:Kk 健康食品
JP2002262820A (ja) * 2001-03-07 2002-09-17 Isao Horiuchi きのこ類の有効成分の抽出方法
JP2003183176A (ja) * 2001-10-09 2003-07-03 Wellness Movement:Kk 免疫賦活組成物

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KR100303886B1 (ko) * 1994-11-19 2001-11-22 임곤혁 상황버섯으로부터항암성분을추출하는방법
JPH11318432A (ja) * 1998-04-30 1999-11-24 Korea Advanced Inst Of Sci Technol 新規のペリヌス属菌株から分離した免疫増強活性多糖類物質及びその使用
JP2002171936A (ja) * 2000-12-05 2002-06-18 Yukito Akiyama 健康食品及びその製造方法
KR20030072417A (ko) * 2002-03-04 2003-09-15 박홍제 버섯 균사체와 균사배양액 유래의 기능성 다당류의 추출법및 추출물을 이용한 기능성 차 또는/및 식품의 제조방법

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104592409A (zh) * 2015-01-12 2015-05-06 陕西师范大学 一种低压低温提取高活性桑黄多糖的方法
US10323263B2 (en) 2015-02-03 2019-06-18 Tate & Lyle Sweden Ab Methods for producing liquid compositions comprising β-glucan
KR101879506B1 (ko) * 2016-10-05 2018-07-17 경북대학교 산학협력단 상황버섯으로부터 베타글루칸의 추출방법
CN106749734A (zh) * 2016-12-26 2017-05-31 庆阳敦博科技发展有限公司 甜百合多糖提取方法

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AU2003256111A1 (en) 2004-11-26
KR20040096000A (ko) 2004-11-16

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