WO2004078155A1 - Use of total flavonoid of bamboo leaf in cosmetic compositions as anti-aging factor - Google Patents

Abstract

This invention relates to the use of total flavonoid of bamboo leaf in cosmetic compositions as anti-aging factor. The object of the present invention is to provide a natural anti-aging factor. Flavonoid extracted from bamboo leaves has effective in antiradical, anti-oxidation, anti-radiation, antimicrobial, bacteria inhibition, promoting proliferation of skin cell, preventing skin from oxidative damage, reducing the formation of melanin, slowing down the process of skin aging and without bringing irritation and allergic reaction to skin and mucosa. It can provide cosmetic compositions with a unique fresh scent of bamboo. It can be used in various kinds of cosmetic compositions such as skin nutrition products, sunscreen, anti-wrinkle and skin-whitening products, face cleaner, bath lotion etc.

Description

 Application of bamboo leaf total flavones as anti-aging skin care factor in cosmetics

 The invention belongs to the field of daily cosmetics. The invention relates to the application of bamboo leaf total flavones as anti-aging skin care factors in cosmetics. As a natural plant-derived anti-aging skin care factor, bamboo leaf total flavones have anti-free radical, antioxidant, anti-radiation, antibacterial, bacteriostatic and whitening, anti-wrinkle, and spotting effects. They can be used in daily cosmetics. application. Background technique

 Cosmetics are very popular commodities, and their consumption follows the trend of the society and has distinctive characteristics of the times. With the deepening of medical research, consumers are more cautious about using cosmetics made of chemical compounds. In recent years, cosmetics have begun to naturalize raw materials, forming a world boom in the development of natural resources. With the improvement of the quality of life that comes with economic development, how to delay the aging process has become the hottest topic. Everyone wants healthy, beautiful and young skin. What is needed is not only to protect the skin, but also to help delay the skin. Aging skin care products. As a result, more and more cosmetics developed to reduce signs of aging appear in the current skin care market.

 Skin is an important part of maintaining human life and health. Direct contact with the outside world is the first barrier for the body, and it is extremely susceptible to damage caused by environmental factors. In addition to aging factors caused by age, stress, pollution, sunlight and ultraviolet radiation are all important causes of wrinkles or wrinkles on the skin.

 Skin aging is mainly manifested in two aspects: (1) the skin gradually loses its elasticity and forms wrinkles; (2) the appearance of the skin gradually produces various pigmentations such as melasma and age spots. According to the theory of aging free radicals, free radical damage to the skin is an important factor in the process of skin aging. Excessive reactive oxygen free radicals in the body interact with unsaturated fatty acids to generate substances such as malondialdehyde (MDA). MDA interacts with proteins on the cell membrane to generate brown pigment, which precipitates on the skin and becomes various pigmentations. Free radicals can also cause the collagen fibers and elastic fibers in the skin to cross-link and denature, become brittle, and lose elasticity. When there is insufficient moisture in the skin, it is easy for the elastic fibers to break and form wrinkles. The deeper causes of skin aging also include slower renewal metabolism of epidermal keratinocytes and reduced proliferation and division of dermal fibroblasts. Compared with young people, the skin of the elderly is thinner, and the skin keratinocytes and fibroblasts are significantly reduced. Therefore, promoting the proliferation of aging skin cells is one of the goals of many skin care products. If the chemical can reduce the generation of skin free radicals, increase the activity of SOD, and have a certain proliferation effect on skin cells, it can accelerate the regeneration of skin tissue, increase the production of collagen fibers, protect the skin's moisture, and effectively slow down the skin. Aging [Huang Hansheng, Anti-aging Skin Care Products, Daily Chemical Industry Translation Series, 1994 (2): 35-38].

The skin care industry agrees that the following two points must be noticed for effective skin care: (1) Wet skin care products should be used at an early age; (2) Skin must be protected from UV rays. Although the skin fine lines are reduced Some progress has been made with the current technology, but the best way is still to delay the appearance of wrinkles and minimize the appearance of wrinkles. Chanel's Mausner believes that the aging of the social population and environmental degradation will cause people's skin to age and become more and more serious, and people will increasingly need long-lasting, highly effective conditioning and preventive skin care products. Elizabeth Arden believes that the trend in the skin care industry is

Cosmeceuticals (meaning functional nutritional cosmetics).

 The age characteristics of the aging population and the special industry background in which cosmetics are located provide a broad space for the application of plant extracts with physiological activities such as anti-free radical, anti-oxidation, anti-radiation, antibacterial, and anti-inflammatory. Coupled with the chain reaction brought by Mad Cow Disease in recent years, animal-derived skin care factors can easily cause people's psychological panic, so the demand for phytochemicals is increasing. There are more than 50 types of plant-based makeup and beauty products launched at home and abroad according to the types of plants added. The specific effects can be summarized as anti-wrinkle, whitening, freckle, itching, moisturizing, weight loss and so on. The extracts of these natural plants have the characteristics of small side effects and good curative effects, and the cosmetic products made have not only maintained the characteristics of the cosmetics, but also have good therapeutic and health effects.

Flavonoids (flavonoids: hereinafter referred to as flavonoids) are widely distributed in the plant kingdom. So far, more than 2,000 species have been discovered, most of which exist in the form of glycosides and a few exist in the free form. Their physiological effects and biological activities are diverse. Diverse. Due to its conjugate structure, it shows strong absorption to both ultraviolet and visible light, and is highly stable in the visible and ultraviolet regions. Naturally derived flavonoids also have antibacterial, anti-photosensitivity, anti-oxidation, detoxification, and whitening effects. They can remove free radicals in the skin, avoid damage to cells by free radicals, promote skin metabolism, improve skin elasticity, and delay skin. The generation of wrinkles, reduces pigmentation, moisturizes the skin, and achieves a significant whitening effect [Wang Jianguo, Liu Haifeng, Wang Jianxin, Sunsol Parsol 1789 Photodegradation inhibition study, Fragrance Cosmetics, 2002 (1): 17-20]. For example, quercetin, which is widely present in vegetables and fruits, has anti-free radical, antioxidant, antibacterial, antiviral and anti-inflammatory, anti-allergic biological activities and pharmacological effects. Luteolin has a strong inhibitory effect on H.Suis virus. At a concentration of 1: 35000, it can inhibit the growth of staphylococcus and subtilis, and it also inhibits catarrh, white rosary, and deforming bacteria. It has anti-inflammatory effects. Compatible with phospholipids can treat dermatitis; strong penetration on the skin, can inhibit hyaluronidase activity, and the use of hyaluronic acid can prolong the physiological activity of the substance; can inhibit and eliminate skin pigmentation (such as age spots). A small amount of luteolin (0.01%) in the nail polish can keep the nail glaze surface smooth. Rutin has anti-oxidant, anti-inflammatory and anti-stomatitis virus effects. Rutin and limonene in a 50: 1 combination have a good inhibitory effect on active oxygen, can strongly absorb ultraviolet rays, can be used for sunscreen, whitening, hair care cosmetics [Wang Jianxin, Natural Active Cosmetics, China Light Industry Press, 1997: 214 ]. Adding 0.1 to 0.2% soy isoflavones to whitening cosmetics can prevent skin aging, blackening and brown spots; adding 2% to hair cream can absorb ultraviolet rays and maintain hair gloss. Delphinium in anthocyanins has strong anti-oxidant and anti-inflammatory effects, and can inhibit bacterial infections caused by skin deficiency caused by excessive sebum secretion in scalp or skin. The addition of cyanogenin in cosmetic water can provide better consistency and smoothness, and has deodorizing and astringent effects. [Wang Qiu'an, Physiological functions of natural flavonoids and their applications, perfumery cosmetics, 1999 (1): 28 -33]. Flavonoids because of It has a variety of activities and its non-toxic and harmless safety and has a wide application prospect in medicines, foods and cosmetics [Huang Xingyu, Ding Jianquan, the role and mechanism of quercetin and its derivatives in cosmetics , Spices, Fragrances, Cosmetics, 1998 (3): 17-18].

 Currently, industrially developed plant flavonoid extracts, such as ginkgo biloba extract, tea polyphenols, grape seed extract, pine bark extract, licorice flavonoids, soybean isoflavones, puerarin isoflavones, etc., many have been used in the cosmetics industry . For example, in the past 30 years, extensive and intensive research has been conducted on ginkgo at home and abroad, and many patented drugs, cosmetics and health products have been developed. [Liu Yan, Chen Jinghua, Application research of ginkgo in anti-aging cosmetics, daily chemical industry, 2002, 32 (4): 79-81]. Ginkgo flavonoids are powerful active oxygen free radical scavengers, which can protect skin cells from the effects of peroxidation, thereby prolonging the life span of skin cells and enhancing their anti-aging ability. Ginkgolides can also accelerate metabolism, improve blood circulation, and enhance Cell viability; In addition, Ginkgo biloba extract also has a broad-spectrum bactericidal effect, and has a significant inhibitory effect on common skin-infecting pathogens such as Staphylococcus aureus, Pseudomonas aeruginosa, and Floccus epidermatis The effect can be displayed at very low concentrations. This highly effective antibacterial characteristic is extremely advantageous for its use as a functional cosmetic ingredient.

[Xiao Chunqian et al., Skin external preparations, Japan: JP-A 5-70333, 1993 -03-23].

 In China, tea extracts have been added to toothpastes and shampoos for more than a decade. Now, Mitsui Agriculture and Forestry Corporation of Japan has launched a variety of products containing tea polyphenols (tea catechins) for food and cosmetics. Flavonoids in tea are mainly flavanols and flavonols, and their main functions are anti-oxidant, anti-cancer, anti-microbial, and deodorant. Catechin belongs to baicalol, which accounts for 20-30% of the dry weight of green tea. The main flavonols in tea include quercetin, tetrahydroxyflavonoids, and myricetin flavonoids, which account for about 2 ~ 3% of the water-soluble extract of tea. The antibacterial and deodorizing effects of catechins delay tooth decay (anti-caries) and improve breath freshness.Therefore, it can be added to toothpaste, mouthwash, chewing gum and breath freshener, and can also be applied to other daily necessities In many shampoos, moisturizing creams, perfumes, and sunscreens, tea extracts are contained. It is believed that they can calm the skin and act as an antioxidant to protect the skin from free radical attacks. [Jia Xudong, Tea Flavonoid Function And its application, Foreign Medical Hygiene Section, 2001, 28 (6): 369-371].

 The main components of puerariae include isoflavones, triterpenoid saponins, and alkaloids, among which the most concerned are isoflavones such as puerarin and daidzein. Isoflavones in Kudzu root can significantly inhibit the catalytic activity of tyrosinase, interrupt the melanin oxidation process, inhibit the occurrence and formation of melanin, and thereby prevent pigmentation such as melasma and sun spots. Therefore, Pueraria lobata is praised by the international cosmetics industry as another skin decolorizing component derived from green plants, and is used in freckle cosmetics by Japan, which is leading in cosmetic technology. [Tang Chunhong, Chen Qi, Current status of research and development of nutritional health functions of Kudzu at home and abroad, China Food Additives, 2002, (6): 56 ~ 58].

Licorice flavonoids in an oil-soluble extract from licorice root have an inhibitory activity against acne on the skin. The development of its water-soluble preparation has also been successful, and the prevention and treatment of acne and acne have been proven on the human body: for testosterone-5a reductase-induced acne and androgen-induced acne (anti-androgen receptor) Both have a preventive effect; it also has a preventive effect on acne caused by acne bacteria, and also has an effect on acne caused by lipase and phospholipase [Yamamoto Jin, information on new raw materials for cosmetics-water-soluble preparation of licorice flavonoids, 2001, ρ12-13]. ·

 Other flavonoids, such as hesperidin, can prevent erythema and skin cancer caused by UV-induced skin cell lipid peroxidation, which means that it can be used as an ideal raw material for sunscreen cosmetics. , 25 (7): 531-533]. The effective ingredients of grass coral contain flavonoid glycosides, coumarins, organic acids, etc. Jiangxi grass coral group company has tested its extracts in products such as shampoo, facial cleanser, shower gel, sun cream and toothpaste [胡国顺 ， 草Coral flow extract and its application in cosmetics, Daily Chemical Industry, 2000 (4): 61-64].

 In view of the urgent demand for cosmetic ingredients derived from natural plants, there is an urgent need in the art to develop new anti-aging skin care factors derived from natural plants. Summary of the Invention

 The purpose of the present invention is to provide a bamboo-derived anti-aging skin-care factor: bamboo leaf total flavonoids. In a first aspect of the present invention, there is provided an application of bamboo leaf total flavones in cosmetics, wherein the bamboo leaf total flavones are applied in cosmetics as an anti-aging skin care factor.

 In a second aspect of the present invention, a cosmetic product is provided, which contains 0.001 to 10 wt% (more preferably 0.005 to 1 wt% of bamboo leaf total flavones.

 In another preferred example, the cosmetics are nutritional beauty lotions (creams), anti-wrinkle whitening honey, sunscreens, facial cleansers, shower gels, and shampoos. BRIEF DESCRIPTION OF THE DRAWINGS

 Figure 1 is the infrared spectrum of bamboo leaf total flavonoids (compressed by potassium bromide);

 Figure 2 is the UV spectrum of bamboo leaf total flavonoids (dissolved in spectrally pure methanol). detailed description

 China is known as the "Kingdom of Bamboo" and has very rich bamboo resources and a long-standing bamboo culture. There are more than 40 species of bamboo belonging to more than 400 species in the territory, and the area of bamboo forests is about 4 million ha. According to incomplete statistics, more than 100 million people in China receive living expenses from bamboo forests and bamboo forest products processing in whole or in part. As an important part of forest resources, bamboo plants not only have high economic value, but also have extensive ecological and social benefits. With its unique biology, ecology, and multi-purpose characteristics, bamboo is increasingly being valued by people, and it is playing an increasingly important role in China's sustainable development strategy. .

China is at the international advanced level in the research and development of bamboo effective ingredients. Bamboo leaf extract is Zhang It is a botanical flavonoid preparation developed in the 1990s. Its invention patent "a health beer with bamboo leaf flavonoid extract (ZL 98 1 04563.4)" and "extract flavonoid compound extract from bamboo leaves or The powder production method (ZL 98 1 04564.2) was authorized by the China National Patent Office in 2000 and 2001, respectively. A large number of studies have shown that bamboo leaf flavonoids have excellent biological effects such as anti-free radical, anti-oxidation, anti-aging, antibacterial, antiviral, protection of cardiovascular and cerebrovascular, prevention and treatment of senile degenerative diseases. And with its rich source of raw materials, clear functional factors, convincing safety, efficient and stable formulation quality, and fresh and sweet bamboo flavor, it has made its debut in the field of functional foods and medical and health products in recent years [张 英 ， natural Functional Additives—Bamboo Leaf Extract, Fine and Specialty Chemicals, 2002, 10 (7): 20 ~ 22].

The functional factors of bamboo leaf extract are mainly C-glycoside flavonoids. The four main bamboo leaf glycoside flavones are Orientin, Homoorientin, Vitexin, and Isolithin Glycoside (Is _0V it _eX in). Compared with oxoflavones, glycoside flavones have the following outstanding advantages: (1) stable structure, not easy to be degraded; (2) can penetrate deep into the lesion site, and directly exert therapeutic effects; (3) enhanced hydrophilicity, which is beneficial to Development of food, medicine and cosmetics. The international academic community has been paying attention to flavonoids since the 1990s, and this field is the latest research front.

Japan's research and development of effective ingredients of bamboo began in the 1970s, but a large amount of work was mainly concentrated on a class of herbal bamboos with characteristics of Japanese resources [known as bamboo grass (English) Bamboo grass, § 卩 Sasa albomarginta Makino & Shibata, etc. ], A small number of ganglia species are involved. Nishina Atsuyoshi et al. 1991 detected 2,6-methoxy-p-benzoquinone from the stem bark of Moso bamboo [J 0 / gn'c & Food Chem, l991, 39 (2): 266-269]; In 1997, he applied for a patent "antibacterial paper containing bamboo leaf extract" [JP Patent 9961697]; in 1998 he also applied for a patent "food antibacterial agent containing V _B1 and bamboo extract" [JP Patent 99269020].

Watanabe Mayumi et al. Applied for a patent of "antibacterial paper containing bamboo grass extract" in 1997 [JP Patent 98310992]. Yamanaka Satoshi et al. (1998) reported the antibacterial activity of bamboo retort and its application

Fudo ¾m: r 1998,14 (9): 57-60]. Nishina Atsuro et al. (1996) filed a patent on "anti-allergic agents containing bamboo extracts" [JP Patent 97278662]. Kiyooka Takatoshi (1995) applied for a patent "" Preparing a deodorant from bamboo hulls using an organic solvent extraction method "" [JP Patent 9794290]. Sato T. et al. Reported in 1986 a bamboo leaf extract solution for the treatment of gums [N ^? O "Shishubyo Gakkai Kaishi, m6, 28 (2): 752- 757]. Especially worth mentioning, Kenji A Japanese patent filed by Matsui et al. (1990) states that the active ingredients extracted from bamboos of the genus Corundum, such as Bamboo, Phyllostachys edulis, Golden Phyllostachys pubescens, using one or more organic solvents can inhibit various dandruff-producing bacteria and prevent skin Aging and inhibiting lipid peroxidation and stabilizing the product [JP Patent 03251518]. However, in this patent document, the source of raw materials refers only to bamboo, not to bamboo leaves or whole bamboo, and it does not say what the active ingredients are. Compounds: So far, Japanese patents and published literature have not clearly pointed out that the active ingredient of bamboo related to the above uses is a flavonoid compound.

Based on previous research, Zhang Ying and his collaborators have recently conducted a systematic research on the functions of bamboo leaf total flavones and skin physiology. It was unexpectedly found that bamboo leaf total flavones are a very good anti-aging skin care factor. The main research results are summarized as follows.

 1 Anti-oxidant and anti-aging effects of total flavonoids in bamboo leaves

Zhang Ying et al. Have studied the biological effects of Mao Jinzhu [· ΡΑ〃 ^ βύ; / ί; $ · nigra var. Hnonis (Bean) Stepf ex Rendle] leaf extract on anti-aging. The results show that the high-dose group can significantly enhance the resistance of mice to non-specific stimulation (normal pressure hypoxia test, p <0.01) and anti-fatigue ability (swim test, p <0.01); learning to normal mice Ability to promote to a certain extent (electric labyrinth method, p <0.05) _; significantly induce the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in aged mice Effect; significantly inhibits the generation of plasma peroxidized lipids (LPO) in elderly mice, and reduces the content of lipofuscin (LF) in the liver; by measuring changes in hydroxyproline content in mouse skin and tail tendon, low Positive effects on collagen were shown at the dose, indicating anti-oxidation, inhibition of lipid peroxidation products, elimination of lipofuscin, and protection of collagen [ZHANG Ying, TANG Lili, Anti-aging effect of Maojin bamboo leaf extract Experimental Research, Bamboo Research Transactions,

1997, 16 (4): 62-67] o

 2 Anti-free radical and anti-radiation effects of total flavones in bamboo leaves

The present invention also by means of experimental weak chemiluminescence and fluorescence spectrophotometry, etc., a comparative study of tea polyphenols, bamboo leaf extract of Ginkgo biloba, and three plant Total Flavonoids Flavonoids Clear _02-- ΌΗ Its activity and ^6Q Co -y irradiation and CuS0 ₄ -Phen-Vc- H ₂ 0 ₂ -DNA system protects DNA damage caused by plutonium.

 2.1 Raw materials

 2.1.1 Sample source

 Tea polyphenols: content 99%, batch number 991227, provided by Professor Yang Xianqiang, College of Agriculture and Biotechnology, Zhejiang University; Ginkgo biloba extract: total flavonoid glycoside content ≥24%, batch number 20000041, produced by Ningbo Chinese Medicine Pharmaceutical Factory; total bamboo leaf flavonoids: total Flavonoid glycoside content ≥24%, batch number 980625, provided by Professor Zhang Ying, School of Biological System Engineering and Food Science, Zhejiang University. The above samples were respectively prepared into a stock solution of 1 mg / mL with three distilled water, and were set aside.

 2.1.2 Drugs and reagents

 Calf thymus DNA, hypoxanthine, xanthine oxidase, luminol, phenanthroline (CPhen), and fluorescent probe (EB) are products of Sigma, USA; potassium nitrate, copper sulfate, PBS and CBS Drugs and reagents are analytically pure; yeast polysaccharide suspensions are made from active dry yeast; Vc and hydrogen peroxide solutions are now in use.

 2.1.3 Instrument

 RF-5000 fluorescence spectrophotometer, produced by Shimadzu Corporation, Japan; BPCL-II ultra-weak luminometer, manufactured by the Institute of Biophysics, Chinese Academy of Sciences; α γ irradiation source was provided by the irradiation center of the Institute of Nuclear Agriculture, Zhejiang University.

 2.2 Experimental methods

2.1.1 Antioxygenation _(02-- and ΌΗ) determining the ability of

The Vc-Cu ²⁺ -H ₂ O ₂ -yeast polysaccharide system was used to generate rhenium, and the hypoxanthine-xanthine oxidase-lumino system was used to generate 0 ₂ _. It was inhibited by samples with appropriate concentration range, ultra-weak light emission. Measure the luminescence value of the blank and test system, calculate the inhibition rate, and further calculate the half-values of 试样 and 0 ₂ _ for different samples by linear regression equation. Inhibition concentration (IC _5. ). '

 2.1.2 Determination of protection against DNA damage

 2.1.2.1 EB-DNA system fluorescence intensity detection method

 In a 1 mL fluorescent cup, add 25 g of NDA and 2 g of EB, and then add a certain concentration of the sample solution, and make up the insufficient part with a 10 mmol / L potassium nitrate solution. After standing for 10 min, measure the fluorescence intensity. The test conditions are: slit Ex = 5nm, Em = 5nm, excitation wavelength 520nm, medium scanning speed, scanning range 550 ~ 720nm, determine the maximum emission fluorescence intensity under different conditions (around 580.8nm), and calculate the sample pair Inhibition ratio I of fluorescence intensity.

 2.1.2.2 Ultra-weak luminescence detection method

10 mL CuS0 ₄ -Phen-DNA solution was prepared with 0.1 mol / L CBS (pH = 10.5) (added 10- ² mol / L o-phenanthroline 490 μ1, 10- ² mol / L CuSO ₄ 70 l, 4 X 10- ⁴ g / mL of ΟΝΑ 35μ1), taking the above solution 858μ1, plus 10_ ² mol / L Vc solution 42μ1, to give a mixture of a volume of 900μ1. After adding samples of different concentrations in the mixed solution, make up the total volume with CBS to 100 μl, and place it in the luminometer measurement room. Finally, the reaction was started with 200 μ1 of 3% Ή ₂ Ο ₂ and the kinetic curve of chemiluminescence was recorded.

 2.1.3 Resistance to DNA radiation damage experiments

Add 0.4 mL of DNA solution (containing 100 g of DNA) to a 5 mL finger shape centrifuge tube, add 0, 20, 40, 80, 16 (^ g / mL different samples), and make up to 2 mL with double distilled water. Each Three parallel samples were set at different concentrations of the sample, and γ-rays were irradiated to the above series of tubes. ^∞ The source intensity of CO was about 144000Ci (April 12, 2001). There were five doses in the experiment, each of which was 0. , 0.8, 2, 8, 20 Gy, but all treatments use the same dose rate, 0.5 Gy / min. After 2.5 hours of irradiation, quickly measure according to the method in 2.1.1.

 2.3 Results

2.3.1 Clearing 〇 ₂ · Comparison with plutonium

 Table 1 Comparison of scavenging performance of three kinds of plant flavonoid extracts on reactive oxygen free radicals

Comparing the ability of the three samples to remove O ₂ -and · ΟΗ, the total flavonoids of bamboo leaves and Ginkgo biloba extract are very close, and tea polyphenols are slightly stronger than them, presumably related to their high purity (Table 1).

 2.3.2 Effect on EB-DNA system fluorescence characteristics

The inhibition of the EB-DNA system fluorescence intensity of the three plant flavonoid extracts has a significant concentration-dependent relationship. From the comparison of IC _5Q values, the total flavones of bamboo leaves and the ginkgo extract are very close, and they are stronger than the tea polyphenols (Table 2). . Table 2 Inhibition rates of flavonoid extracts from three natural plants on the fluorescence intensity of the EB-DNA system

 Flavonoids may compete with EB for binding sites on DNA molecules and pull down EB molecules embedded in DNA base pairs. In this way, the higher the concentration of the sample, the fewer EB molecules bound to the DNA, and the EB -The lower the fluorescence intensity of DNA.

 2.3.3 Protective effect against DNA radiation damage

Table 3 Effect of γ-irradiation at different doses on the fluorescence characteristics of EB-DNA

 Table 3 shows that irradiation can have direct and indirect effects on the DNA solution. Direct effects can cause DNA molecule hydrogen bond destruction, double helix unraveling, base shedding, single and double-strand breaks, etc. Indirect effects are mainly due to free radicals generated during irradiation, especially tritium, which attack the DNA strands and cause DNA molecules Damage. As a result, the binding sites of EB and DNA are reduced, and the fluorescence intensity value is also reduced.

Table 4, Table 5 and Table 6 show the effects of different kinds and different concentrations of samples on the EB-DNA fluorescence intensity at different doses. Flavonoids can effectively scavenge free radicals, and therefore have a significant protective effect on the free radical damage of DNA molecules caused by irradiation. Tea polyphenols, ginkgo biloba extract, and bamboo leaf total flavones can maintain the fluorescence intensity of EB-DNA at a high level at different concentrations in the range of 20 ~ 160μ _β / ηΛ, indicating that they have protection DNA irradiation damage effects and show concentration dependence. From the perspective of the protective effects of the three extracts, tea polyphenols were slightly stronger than the ginkgo extracts and bamboo leaf total flavones, and the latter two were close.

表 5不同浓度的银杏提取物对不同剂量辐照时 EB-DNA荧光强度的影响

Table 5 Effect of different concentrations of Ginkgo biloba extract on EB-DNA fluorescence intensity under different doses of irradiation

 Table 6 Effect of different concentrations of total flavones in bamboo leaves on EB-DNA fluorescence intensity under different doses of irradiation

2.3.4 Effect of CuSO ₄ -Phen-Vc-H ₂ O ₂ -DNA chemiluminescence kinetics

The chemiluminescence system of CuSO ₄ -Phen-Vc-H ₂ O ₂ -DNA is in an alkaline medium, and phenanthroline (Phen) can react with H ₂ O _{2 to} generate chemiluminescence under the catalysis of metal ions. The emission wavelength is in the range of 445 ~ 450nm. According to relevant literature reports, 0 ₂ · is the main factor that causes Phen to generate chemiluminescent substances, and it is in the front peak position on the luminescence kinetic curve. At the same time, the plutonium generated in the system causes DNA damage and breakage, and produces a slow chemiluminescence that is delayed from the light emission of Phen itself. The maximum emission wavelength is between 380 ~ 420nm. This luminescence is a characteristic reaction of free guanine. The top is in the rear peak position.

 In the presence of three samples of tea polyphenols, ginkgo biloba extract, and total flavonoids of bamboo leaves, the peak value of the front peak and the peak of the rear peak decreased significantly, and its semi-inhibitory concentration was about 0.2 g / mL. The decrease in the luminescence peak is due to the elimination of ΌΗ and the like that initiated the chain reaction by the antioxidant, which reduces the DNA damage caused by ΟΗ and the like. It is believed that the flavonoid acts as a preventive antioxidant. At the same time, it can be seen that the appearance time of the front peak value has almost no change, and the latter peak value lags behind, and with the increase of the antioxidant concentration, the peak backward time is prolonged. It is thought that the post-peak emission peak delay is caused by the sample removing the intermediate free radicals of the free radical chain reaction, interrupting the chain reaction, and protecting the DNA from free radical damage. Antioxidants. It has been proved that flavonoids such as tea polyphenols, ginkgo biloba extract, and total flavonoids in bamboo leaves act as both primary and secondary antioxidants.

 3Antibacterial and anti-inflammatory effects of total flavonoids in bamboo leaves

 3.1 Experimental raw materials

3.1.1 Strain source Enterococcus faecalis (E.faecalis), S. pyogenes (S. pyogenes), Epidermidis (S. epidermidis), P. vulgaris (P. vulgaris), K. pneumoniae (K. pneumoniae) from Zhejiang Province From the clinical urogenital tract specimens of the People's Hospital, they were isolated within 3 months before the experiment. Standard strains S. aureus (ATCC25923) and E. coli (ATCC25922) were provided by Zhejiang Provincial Clinical Test Center.

 3.1.2 Experimental animals

 ICR mice (clean grade), weighing 18 ~ 21g, males, were provided by Zhejiang Experimental Animal Center.

 3.1.3 Sample

 Total flavonoids in bamboo leaves: The total flavonoid glycoside content is ≥50%. It is a freeze-dried tan-like crystalline powder, stored in a desiccator, and prepared with distilled water before use.

 3.1.4 Drugs and reagents

 2% croton oil: Formulated with 2% croton oil, 20% absolute ethanol, 5% distilled water, and 73% diethyl ether when in use; Indomethacin: API, gifted by Ningbo Pharmaceutical Factory, 1% CMC when used -Na suspension formulation; Xylene: Hangzhou Chemical Reagent Factory; MH broth and MH agar were purchased from the Logistics Inspection Factory of Zhejiang Military Region; 0.22μm bacterial filter membrane was produced by the American company Milipore; newborn calf serum: super No mycoplasma, purchased from Hangzhou Sijiqing Bioengineering Materials Research Institute.

 3.1.5 Equipment

Shell / JB 3200 CO ₂ culture phase, product of the American Sheldon company; DG-3022A enzyme-linked immunoassay, produced by East China Electron Tube Factory; COIC biological inverted microscope, manufactured by Chongqing Optical Instrument Factory; SC-1 horizontal laminar flow purification Workbench, manufactured by Suzhou Institute of Clean Technology; CQ250 ultrasonic cleaner, produced by the 726 Ultrasonic Instrument Factory of the Seventh Hospital of China Shipbuilding Corporation.

 3.2 Methods and results

 3.2.1 Bacteriostatic effect

Streptococcus pyogenes is performed according to the drug liquid dilution method: 10% of inactivated calf serum is added to the sterilized MH broth, and then the sample is added to make a concentration of 25mg / mL, and the bacteria filter is installed in a sterilized large syringe. Sterile filtration treatment. The sterile filtrate was diluted twice with sterile MH serum medium, 1 mL per tube. Add 0.05mL of young Streptococcus pyogenes cultured for 4 to 6 hours (total bacteria content is 10 ⁴ cfu) and incubate in a 35 ° C incubator for 20 to 24 hours. Tube as its MIC value.

Other bacteria are carried out by double dilution of the drug: after dissolving each vial of MH agar, autoclaving and dissolving, cooling to about 55 ° C, adding different amounts of samples to make them 25.0, 12.5, 6.25, 3.125, respectively. , 1.56, 0.78 and 0.39 mg / mL concentration, set a MH agar without drug as a positive growth control dish after adding bacteria. Then add 6 kinds (see 3.1.1 strain source) bacterial solution of turbidimetric concentration to each dish (total bacterial content is 10 ⁴ cfu), and use the inoculation ring to paint small garden spots (both are 4 ~ 6h juvenile bacteria), cover the dish after drying, and incubate at 35 ° C for 16 ~ 20h. Use the lowest sample concentration tube with no bacterial growth as its MIC value. Table 7 Antibacterial effect of total flavonoids in bamboo leaves

 The results showed that bamboo leaves total flavonoids had different degrees of inhibition on the above seven bacteria (Table 7).

 3.2.2 Anti-inflammatory effect

 3.2.2.1 Effects of croton oil-induced auricle swelling in mice

 Mice were randomly divided into 5 groups according to body weight, 12 in each group. The settings were as follows: ① Control group: equal volume of normal saline, 0.8mL / 20g; ② Indomethacin group: indomethacin 10mg / kg; ③ high-dose group: 10mg / mL BLTF-01 at a dose of 400 mg / kg; ④ Medium dose group: 5 mg / mL BLTF-01 at a dose of 200 mg / kg; ⑤ Low dose group: 2.5 mg / mL BLTF-01 at a dose of 100 mg / kg. Animals were po-administered once a day for 7 days (indomethacin was administered only once), and lh after the last administration, mice were coated with 2% croton oil 0.02mL / head in the right ear, causing inflammation, and sacrificed after 4h, along the auricle Cut the left and right ears, punch the left and right ear pieces with a 9mm ring drill, weigh them, and record the difference between the weights of the left and right ear pieces as the swelling rate.

 The experimental results showed that mice were given 200 mg / kg and 400 mg / kg of bamboo leaf total flavonoids by gavage for 7 consecutive days, and had significant (* p <0.05) and extremely significant (p <0.01) on croton oil-induced auricle swelling. The inhibitory effect was significant and showed a significant dose-dependent relationship (Table 8).

 Note: Compared with the control group, * p <0.05; ** p <0.01.

 3.2.2.2 Effect on mouse xylene-induced auricle swelling

Mice were randomly divided into 5 groups according to body weight, with 10 mice in each group. The setting was the same as 3.2.2.1. Animals were administered once a day for 7 consecutive days (indomethacin was administered only once), and 30 minutes after the last administration, the mice's right ear was coated with xylene 0.05 mL / head, causing inflammation, After 15 minutes, the rats were sacrificed, the left and right ears were cut along the auricle, and the left and right ear pieces were punched out with a 9mm ring drill, weighed, and the difference between the weights of the left and right ear pieces was recorded as the swelling rate. Compare with the control group to judge the curative effect.

 Note: Compared with the control group, * ρ <0.05; ** ρ <0.01.

 The experimental results showed that mice were given a total of 400 mg / kg of bamboo leaf total flavones by gavage for 7 consecutive days, which had a significant (* p <0.05) inhibitory effect on auricle swelling caused by xylene. (Table 9).

 4Skin Physiological Activity of Bamboo Flavone

 4.1 Experimental materials and methods

 4.1.1 Sample

 Bamboo leaf total flavonoids: brownish yellow powder with a total flavonoid content of 28.7%. The sample was dissolved in DMSO, filtered through a 0.22 μm filter to remove bacteria, and diluted with serum-free DMEM to the test concentration. The final concentrations were 0.5%, 0.05%, and 0.005%, and stored at -4 ° C. Serum-free DMEM was used as a control.

 4.1.2 Reagents, utensils, instruments

 DMEM medium, keratinocyte (K-SFM) medium (Gibco BRL), insulin (American Sigma), trypsin (American Difco) calf serum (NBS, Hangzhou Sijiqing Company), tetramethyl coupling Azole (MTT, Fluka Corporation, USA), dimethyl sulfoxide (DMSO, Shanghai Institute of Biochemistry); cortisone, penicillin, streptomycin, Ficoll, 96-well plate and 24-well plate, and 35mm culture dish and 25cm2 culture flask (Coming, USA). Microplate reader (BIO-TEK), pH meter, clean bench, incubator, 722 spectrophotometer.

 4.1.3 Cell Culture

 4.1.3.1 Primary culture of skin keratinocytes

 Peel off the back skin of SD rats (3 days old) under sterile conditions, and place the skin in a refrigerator at 4 ° C for 30 ~

Adhere to the wall for 60 minutes, slowly add 0.25% trypsin vertically and slowly in a refrigerator at 4 ° C overnight (10 ~ 1 lh), then rinse with PBS, and neutralize the trypsin with 10% NBS DMEM medium. Separate the epidermis from the dermis, brush the keratinocytes at the base layer of the epidermis, and centrifuge to remove the fibroblasts. The cell density was adjusted to 1 × 10 mL with K-SFM medium, seeded in 96-well plates and 24-well plates, and the first solution was changed after 24 h, and the solution was changed every Id thereafter. After the cells grew to a sub-fusion state, samples of each concentration were added after starvation for 24 hours. 4.1.3.2 Primary Culture of Skin Fibroblasts

 Digest the isolated dermis for 90 minutes, neutralize the trypsin with DMEM containing 10% NBS, cut and pipette in DMEM, filter with 100 mesh filter, wash the cells twice, and adjust the cells with DMEM culture solution containing 10% NBS Density to 2 X 10VmL, seeded in% well plate. After 24h, change the first liquid, and then change the liquid every 2 ~ 3d. When the cells grew to 80% confluence, test substances of various concentrations were added after 24 h of starvation.

 4.1.3.3 B-16 melanoma cell culture

The number of cells was adjusted to 1 × 10 ⁴ cells / ml with DMEM medium containing 15% NBS, and inoculated into culture flasks and 35 mm petri dishes.

 4.1.4 Cell Proliferation Assay

The 96-well plate was seeded with 2 × 10 ⁵ cells per well. After the cells were cultured in a sub-fusion state, the solution was changed and test substances of various concentrations were added. Each concentration was 4 samples. After adding the drug, 10 μΙ MTT was added to each well for 24 hours, the culture was continued for 4 hours, and the supernatant was discarded. After adding 100 μΙ of the lysate, it was detected on a microplate reader at 570 mm and 630 nm.

 4.1.5 Determination of Lipid Peroxidation Product (MDA) and Superoxide Dismutase (SOD)

 MDA was determined by TBA colorimetry, SOD was determined by nitrite reduction, and the kit was provided by Nanjing Jiancheng Biological Company.

 4.1.6 Determination of melanocyte tyrosinase activity and synthetic melanin

 4.1.6.1 Determination of tyrosinase activity

The density of B 16 cells was adjusted to 1.0 × 10 ⁴ cells. Each 35 mm culture dish was inoculated with 1.0 mL of a cell suspension and cultured for 24 h. At the cell doubling phase, test substances of various concentrations were added, the cells were harvested at 24 h, washed twice with PBS, 2.0 M1 PBS (containing 0.1% Triton-X) was added, and the cell debris solution was removed at 37 ° C for 30 min. 1mL for protein determination. Add 2.0 mL of PBS containing 0.1% L-DOPA to the remaining solution, incubate at 37 ° C for 1 h, and determine the colorimetry at 400 nm. -'

 4.1.6.2 Determination of melanin synthesis

The cell density was adjusted to 1.0 x 10 ⁴ cells, and the cells were seeded in a culture flask for 24 hours, the test substance was added, the L solution was changed at 48 h, and the cells were harvested at 72 h. The cells were washed with PBS and trypsinized. The collected cells were lysed in 2 mL of PBS, and 0.2 mL was taken out to determine the protein content. To the remaining 0.9 mL of the cell fluid, 1.8 mL of 4 mol / L NaOH was added, and the mixture was heated at 100 ° C for 20 minutes, and the colorimetric measurement was performed at 400 nm.

 4.1.7 Safety evaluation test

 According to the safety evaluation procedures and methods in the "Cosmetic Hygiene Regulations" issued by the Ministry of Health in 1999. The test substance concentration is 10%. Skin irritation test: Take 0.2ml of test substance and apply it on the skin. Once a day for 1 h each time for 14 consecutive days. Eye irritation test: 0.1 ml of the test substance was dropped into the conjunctival sac, and the eyes of the animals were tested at 1, 24, 48, 72 h, and on the 4th and 7th days after exposure. an examination.

 4.2 Results

4.2.1 Proliferation effect on skin cells Table 10 shows that the total flavonoids of bamboo leaves promote skin cell proliferation in a dosage range of 0.005% to 0.05%, wherein the proliferation effect on skin keratinocytes is significant at a concentration of 0.005% (p <0.05) and at a concentration of 0.05% Very significant (p <0.01); there was also a significant difference in the proliferation of skin fibroblasts compared with the control group (at 0.005%, p <0.05).

 Table 10.Effect of bamboo leaves total flavonoids on the proliferation of two types of skin cells

 Note: * p <0.05, ** p <0.01, compared with the control.

 4.2.2 Effect on MDA production and SOD activity of skin cells

 It can be seen from Table 11 that the total flavonoids of bamboo leaves can reduce the production of lipid peroxidation product MDA and increase the activity of antioxidant enzyme SOD in the dosage range of 0.0005% ~ 0.005%. <0.05). '

 4.2.3 Skin whitening effect

 B16 melanoma tumor cell tyrosinase inhibition test results show that the total flavonoids of bamboo leaves have no significant effect on the enzyme activity; and from the results of melanin synthesis tests, it has been shown that the concentration of melanin is significant at 0.005% ~ 0.05% Inhibition (see Table 12).

 Table 11 Effects of bamboo leaf total flavonoids-dermal keratinocytes on MDA production and SOD activity

 Sample concentration (%) MDA (nmol / mL) SOD (U / mL) Blank control 1.66 + 0.22 2.94 ± 0.085

 0.0005 1.40 + 0.53 3.00 ± 0.17

0.005 1.13 ± 0.13 * 3.29 ± 0.16 *

0.05 2.26 ± 0.81 2.49 + 0.16 Note: * p <0.05, compared with the control. Table 12 Bamboo leaf total flavones-B16 melanoma tumor cell tyrosinase activity and the effect of melanin synthesis Sample concentration (%) tyrosinase activity melanin synthesis

 (OD / mg protein) (OD / mg protein) Blank control 5.81 ± 0.31 1.72 ± 0.38

 0.0005 6.50 + 0.47 1.12 ± 0 · 23

0.005 6.83 ± 1.34 1.05 + 0.13 *

0.05 6.29 ± 0.54 0.78 ± 0.07 **

0.5 5.28 ± 2.40 2.00 ± 0.56 Note: * p <0.05, ** p <0.01, compared with the control.

 4.2.4 Skin safety evaluation test

 The skin irritation test and eye irritation test of bamboo leaf total flavones were negative, showing no skin and eye irritation.

 4.3 Conclusion

 The skin irritation test and eye irritation test are to understand whether foreign substances have irritating and damaging effects on the skin or cause inflammatory lesions of the mucosa in the case of contact with the skin or mucous membranes. They are mandatory inspection items for the safety evaluation of cosmetics and their raw materials. The test results showed that the skin and eye conjunctival irritation response of total bamboo leaf flavonoids were negative, indicating that under external skin application conditions, it would not cause irritation to human skin and mucous membranes. Tests show that bamboo leaves total flavonoids have good biological effects on skin cells, can promote the proliferation of skin keratinocytes and fibroblasts, increase SOD activity, and reduce oxidative damage, suggesting that bamboo leaves total flavones can be used to delay skin aging. The active substance is applied to cosmetics. The skin whitening effect test found that bamboo leaves total flavonoids can reduce the amount of melanin synthesized by melanocytes, suggesting that it may have a whitening effect on the skin.

 The total flavones of bamboo leaves referred to in the present invention are flavonoid preparations with different accuracy obtained from the leaves of Graminae, Bambusoideae: and Phyllostochys Sieb. Et Zucc varieties, and the production process thereof It has been involved in two previous invention patents of Zhang Ying (patent numbers ZL 98 1 04564.2 and ZL 98 1 04563.4). It should be noted that the bamboo leaf total flavonoids referred to in this patent can be either products obtained by using the above patent process, or based on this, further use of high-tech technologies such as adsorption-desorption, membrane separation, supercritical fluid extraction and the like The obtained bamboo leaf flavonoid product is refined by a combined method.

The appearance of total flavonoids in bamboo leaves is yellow or brownish-yellow powder (also available in the form of extract), and the total flavonoid glycoside content (on a dry basis) can vary between 10 and 90%, among which humengin, isohumuloside The content of the four main glucosides, vitexin, and isovitein is more than 50% of the total flavonoid glycosides. The infrared spectrum of the potassium bromide tablet shows that bamboo leaves total flavonoids have characteristic absorption near 3400, 2900, 1610, 1520, 1080cm- ¹ and so on (see attached picture 1); after dissolving them in pure methanol Scanning in the wavelength range of 200 ~ 600mn, the ultraviolet spectrum shows that there are two main absorption peaks in the 240 ~ 400nm region, of which there is a strong absorption peak between 240 ~ 280nm, and once between 300 ~ 350nm Strong absorption peak, which meets the typical characteristics of flavonoids (see Figure 2).

Identification of chemical reagents for total flavonoids in bamboo leaves: Take 0.5g of the sample and dissolve it in 100mL of 95% ethanol. ① Take 1mL of the above solution, and force 2 ~ 3 drops of 1% FeCl ₃ -ethanol solution. It should be dark blue or blue-violet. ② Take 1mL of the above solution, add 2 ~ 3 drops of 1% A1C1 ₃ -ethanol solution, it should be bright yellow. + Take 0.5g of BLTF, add 10mL of ether, ultrasonic-assisted extraction for 30s, and filter. Take 1mL of the filtrate, place it in a water bath at 70 ~ 90 ° C, and dry the ether, then add 2% m-dinitrobenzene solution (prepared with 95% ethanol) and 1mL of 2.5mol / L KOH aqueous solution, which should appear immediately Slightly reddish, when placed in the hot water bath above, it quickly turns deep purple. The latest research involved in the present invention shows that the total flavonoids of bamboo leaves have no irritating and allergic reactions to the skin and mucous membranes; they have anti-free radical, antioxidant and anti-radiation activities comparable to tea polyphenols and ginkgo extracts;

Within the dosage range of 0.005% ~ 0.05%, it can significantly promote the proliferation of skin cells and significantly inhibit the synthesis of melanin; within the dosage range of 0.0005% ~ 0.005%, it can significantly reduce the production of MDA and increase the activity of SOD It has sufficient and necessary conditions to be used as a safe, efficient, and economic phytochemical as an anti-aging skin care factor.

 In view of the anti-free radical, antioxidant, anti-aging, anti-radiation, antibacterial, antibacterial, anti-inflammatory, whitening, anti-wrinkle functions of bamboo flavonoids, it also meets the safety requirements as a cosmetic additive, and can be used as The functional factors of anti-aging skin care cosmetics are applied in various daily cosmetics such as nutrient moisturizers, sunscreen skin care agents, anti-wrinkle whitening agents, cleansing milks, shampoos, shower gels and so on.

 In addition to the functional ingredients in the bamboo leaf total flavonoids, the skin-care factor of the present invention can be appropriately blended with components commonly used in cosmetics to make products for various uses. These other components can include various vitamins and their derivatives, hyaluronic acid, surfactants, wetting agents, chelating agents, pH adjusters, excipients, other UV absorbers, preservatives, dyes, fragrances, etc. .

 The cosmetics of the present invention can be made into various forms, such as oily cosmetics, emulsion cosmetics, and water-based cosmetics.

 The cosmetics of the present invention have cosmetic skin care effects such as whitening, anti-wrinkle, sun protection, anti-inflammatory, mottled spots and cowpea. The main advantages of the present invention are: It provides a broad-sourced, safe, efficient, and economically applicable anti-aging skin-care factor—total flavonoids from bamboo leaves, and systematically studies its characteristics related to the physiological functions of the skin, showing that it has anti-freedom Base, anti-oxidation, anti-radiation, antibacterial, bacteriostatic, promote skin cell proliferation, prevent skin oxidative damage, reduce melanin synthesis, delay skin aging and other beauty effects, and can give the product a unique fragrance of bamboo, such as nourishing and nourishing Agents, sunscreen skincare agents, anti-wrinkle whitening agents, cleansing milk, shampoo, shower gel and other daily cosmetics fields have broad application prospects.

 The invention will be illustrated by the following non-limiting examples. Units in the following formulation examples are weight percent (w /%).

 Example 1 Nutritional Lotion (Cream)

 Beeswax 13 ~ 17

 Refined floor wax 13 ~ 17

 Vaseline 15 ~ 20

 Lanolin 3 ~ 7

 Olive oil 8 ~ 12

 Polyoxyethylene sorbitan monolaurate 2 ~ 6

 Spices 0.1 ~ 1

Bamboo leaf total flavonoids 0.005 ~ 0.05 Add to 10CK

 This product has antibacterial, cosmetic, improving blood circulation, promoting metabolism, enhancing skin vitality, moisturizing the epidermis, inhibiting the secretion of abnormal sebum, and activating cells and anti-tissue toxicity.

 Example 2 Anti-wrinkle whitening honey

 This product has anti-wrinkle, whitening, moisturizing, and spotting effects.

 Example 3 Sunscreen

 This product has sun protection, anti-aging and skin care effects.

 Example 4 facial cleanser

 Vitamin E and its derivatives 0.05 ~ 0.5

 Glyceryl tristearate 6 ~ 10

 Cetyl Alcohol 1 ~ 3

 Liquid paraffin 8 ~ 16

Bamboo leaf total flavones 0 · 01 ~ 0 · 05 Triethanolamine 1 ~ 3

 Propylene glycol 4 ~ 8

 Add water to 100.

 This product has bacteriostatic, inhibited secretion of sebum secretion, anti-inflammatory, cowpea and so on.

 Example 5 shower gel

 This product can inhibit harmful bacteria on the skin surface, activate skin cells, condition the skin and prevent skin cancer, and make the body with a pleasant fragrance for a long time after bath.

 Example 6 Shampoo

 In addition to making the hair shiny, soft and easy to comb, this product can also prevent the loss of keratin during shampooing, prevent the yellowing and bifurcation of the hair, prevent itching of the scalp, and protect the hair. Hair has a light fragrance for a long time. All documents mentioned in the present invention are incorporated by reference in this application, as if each document were individually incorporated by reference. In addition, it should be understood that after reading the above-mentioned teaching content of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

 Rights request 1. The application of bamboo leaf total flavonoids as an anti-aging skin care factor in cosmetics, which is characterized in that the bamboo leaf total flavones are applied as an anti-aging skin care factor in cosmetics.  2. A kind of cosmetics, which is characterized in that it contains 0.001 to 10% of bamboo leaf total flavonoids.  The cosmetic according to claim 2, characterized in that the cosmetic is a nutritional cosmetic lotion (cream), an anti-wrinkle whitening honey, a sunscreen, a facial cleanser, a shower gel, and a shampoo.  The cosmetic according to claim 3, wherein the weight percentage of the nutritional cosmetic lotion (cream) is:  Beeswax 13 ~ 17  Refined floor wax 13 ~ 17  Vaseline 15 ~ 20  Lanolin 3 ~ 7  Olive oil 8 ~ 12  Polyoxyethylene sorbitan monolaurate 2 ~ 6  Spices 0.1 ~ 1  Bamboo leaf total flavones 0.005-0.05  Add water to 100.  5. The cosmetic according to claim 3, wherein the weight percent of the anti-wrinkle whitening honey is grouped into:  L-ascorbic acid and its derivatives 1 ~ 3  Olive oil 10 ~ 20  Isopropyl myristate 3 ~ 7  Nonylphenol polyoxyethylene ether 0.1 ~ 1  Glycerin 3 ~ 7  Hyaluronic acid 1 ~ 3  Bamboo leaf total flavones 0.005-0.05  Ethanol 5 ~ 10  Hydraulic port 100.  6. The cosmetic according to claim 3, wherein the weight percentage of the sunscreen is  Cetyl to stearyl alcohol 6 to 10  White oil 26 * 3 ~ 6 Isopropyl palmitate 3-6 Dimethicone  Monoglyceride  Lanolin  Polyethylene glycol -6000 glycerin

 Bamboo leaf total flavonoids. 0.005 ~ 0.05 SPP-200 (phosphonate quaternary ammonium cationic emulsifier) 2 ~ 3  Flavor preservatives 0.1 ~ 1  Add water to 100.  7. The cosmetic according to claim 3, characterized in that the weight fraction of the face wash is vitamin E and its derivatives 0.05 to 0.5 glyceryl tristearate 6 to 10  1 ~ 3  Liquid paraffin 8 ~ 16  1  Bamboo leaf total flavonoids 0.01-0.05  2  Triethanolamine 1 ~ 3 1 1  Propylene glycol 4 ~ 8  Add water to 100.  The cosmetic according to claim 3, characterized in that said shower gel has a weight percentage of amphoteric lauric acid hydroxypropyl sulfonate 20 ~ 30  Coconut amide propyl hydroxypropyl sulfonated betaine 7 ~ 13  Lauryl ether sulfate (30%) 8 ~ 12 Laurylamido diethanolamine 0.5 ~ 1.5 Bamboo leaves total flavones 0.01 ~ 0.5 Water is added to 100. The cosmetic according to claim 3, wherein the weight percentage of the shampoo is

 Sodium lauryl sulfate 40 '  Hydroxypropyl cellulose 0.5  Oleyl diethanolamide Cationic animal protein Polyethylene glycol lanolin 1 ~ 3  Methyl paraben 0.1 ~ 0.3 Quaternary amine compound -15 0.05 ~ 0.15 Polyaminopropyl biguanide chloroxylenol 0.05 ~ 0 · 15 Soy phospholipid 0.1 ~ 1 Total flavonoids in bamboo leaves 0.01—0.5 Water Add to 100ο 10. The cosmetic according to claim 2, further comprising 0.005 to

Flavonoids.