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WO2004073828A1 - Procede d'extraction de chlorophylle a partir d'excrements de ver a soie et composition ainsi produite - Google Patents

Procede d'extraction de chlorophylle a partir d'excrements de ver a soie et composition ainsi produite Download PDF

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Publication number
WO2004073828A1
WO2004073828A1 PCT/KR2004/000348 KR2004000348W WO2004073828A1 WO 2004073828 A1 WO2004073828 A1 WO 2004073828A1 KR 2004000348 W KR2004000348 W KR 2004000348W WO 2004073828 A1 WO2004073828 A1 WO 2004073828A1
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WO
WIPO (PCT)
Prior art keywords
chlorophyll
mixture
stirring
concentrate
produce
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/KR2004/000348
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English (en)
Inventor
Chang-Ho Jeon
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Individual
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Individual
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Filing date
Publication date
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Publication of WO2004073828A1 publication Critical patent/WO2004073828A1/fr
Anticipated expiration legal-status Critical
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Classifications

    • E—FIXED CONSTRUCTIONS
    • E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D17/00—Excavations; Bordering of excavations; Making embankments
    • E02D17/20—Securing of slopes or inclines
    • E02D17/202—Securing of slopes or inclines with flexible securing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents
    • E—FIXED CONSTRUCTIONS
    • E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
    • E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
    • E02D2300/00—Materials
    • E02D2300/0051—Including fibers
    • E02D2300/0065—Including fibers made from textile

Definitions

  • the present invention relates to a chlorophyll extraction method and a composition produced thereby, and more particularly, to extracting chlorophyll concentrate from fine powders of silkworm excrements by the addition of extraction solvent, and producing liquid and powdery chlorophyll compositions from the chlorophyll concentrate.
  • Chlorophyll is generally present in a chloroplast, and it is most abundantly distributed in leaves and sometimes also in green parts of other tissues, such as stems and roots.
  • the chlorophyll absorbs sunlight and converts the light energy into chemical energy by a photosynthesis process.
  • the converted energy transforms carbon dioxide (C0 2 ) and water into carbohydrates, such as glucose or starch, to supply nutrition to plants.
  • C0 2 carbon dioxide
  • carbohydrates such as glucose or starch
  • the chlorophyll When carefully examining the chemical structure and components of chlorophyll, the chlorophyll is a wax-like, blue and red fine crystal with about 5 ⁇ m size, and is a chelate form with magnesium (Mg) .
  • the chlorophyll emits fluorescence when exposed to ultraviolet rays in a dark place, and chlorophyll-a and chlorophyll-b are generally found in higher plants at a ratio of 3:1-3:2.
  • the chlorophyll extract according to the present invention contains tocopherol (vitamin E) , fatty acid (e.g., linoleic acid), carbohydrate, vegetable fat and carbon powder, the chlorophyll is difficult to store at room temperature and easily decomposed at room temperature, and thus should be freeze-stored. Thus, it has problems in terms of storage and distribution.
  • an object of the present invention is to provide a chlorophyll extraction method in which the excrements of fifth-instar silkworms is dried, washed, sterilized and pulverized into fine powders, the fine powders are added only with extraction solvent and then stirred, the stirred solution is passed through a centrifuge and a vacuum concentrator without using an ultrasonic system, to extract chlorophyll concentrate, the extracted chlorophyll concentrate is emulsified without using the ultrasonic system, and from the emulsified chlorophyll concentrate, a liquid or powdery composition is produced, and also to provide liquid or powdery compositions produced by the above method.
  • FIG. 1 shows the chemical structure of chlorophyll to be produced according to the present invention
  • FIG. 2 is a flow diagram showing the extraction and emulsification processes of chlorophyll according to the present invention
  • FIG. 3 is a flow diagram showing a process for producing a liquid chlorophyll composition according to the present invention
  • FIG. 4 is a flow diagram showing a process for producing a powdery chlorophyll composition according to the present invention.
  • the collected excrements are washed clean with water in a spraying manner, and sterilized with ultraviolet rays and ozone (O 3 ) , and then pulverized into fine powders.
  • Starch raw materials such as corns, sweet potatoes and potatoes, are fermented in the pulverized excrements, and a distilled and purified ethyl alcohol having a 95% alcohol concentration is added to the fermented product at the amount of 50-70% by weight relative to the excrements.
  • the mixture is introduced into a stainless container which is not oxidized by strong acid and strong alkaline.
  • the introduced material is stirred at high speed, and separated into a first chlorophyll extract and a residue by a centrifuge.
  • methylene chloride (dichloromethane; CHCI 2 ) with excellent extraction and volatilization properties are added at the amount of 40- 70% by weight relative to the residue, and the mixture is stirred in a stainless container at high speed, and then separated into a second chlorophyll extract and a residue.
  • the first and second chlorophyll extracts are stirred while volatilizing the extraction solvent.
  • the remaining chlorophyll extract is vacuum-concentrated to produce chlorophyll concentrate.
  • the chlorophyll concentrate produced is emulsified in the following manner.
  • the chlorophyll concentrate is stirred in a stainless container which is not oxidized by strong acid and strong alkaline. During the stirring step, the chlorophyll concentrate is emulsified with fatty acid, hydrogenated oil from animals and plants, and fatty acid ester.
  • emulsifiers corn oil, polyoxyethylene sorbitol fatty acid ether and polyoxyethylene glycerin fatty acid ester are used to emulsify the chlorophyll concentrate (see FIG. 2) .
  • water containing no minerals is prepared from tap water by a reverse osmosis system.
  • the prepared water has a pH below 7.
  • silver-ion water is made by a colloidal silver ion water-making system.
  • silver in the silver-ion water has excellent bactericidal ability against various bacteria, such as E . coli and virus, and has the ability to sterilize about 650 kinds of bacteria within at least 6 minutes.
  • the silver-ion water-making system which is used in the present invention, is a colloidal silver-ion preparation system, in which silver microparticles with a purity of
  • liquid chlorophyll composition 99.99% and a size of 0.005-0.015 microns are dissolved in 800 cc of distilled pure water at a concentration of 3-5 ppm. added to the colloidal silver-ion water prepared by the silver-ion water-making system, and stirred in a glass or plastic container at high speed, thereby producing a liquid chlorophyll composition.
  • the liquid chlorophyll composition produced as described above does not to be stored in a refrigerator during its distribution, because the silver contained in the liquid chlorophyll composition continuously sterilizes bacteria and inhibits the production of bacteria.
  • the colloidal silver was a natural bactericidal (antibiotic) agent sterilizing substantially all single-cell pathogenic organisms found on the ground. It is the most powerful theory that the silver inactivates special enzymes with metabolic actions (digestion and breathing) in the single-cell pathogenic organisms, thereby sterilizing the organisms .
  • Example 1 Production of liquid chlorophyll composition Chlorophyll concentrate is extracted from the excrements of fifth-instar silkworms using extraction solvent.
  • an extraction solvent with 95% alcohol concentration is added to 160 kg of silkworm excrements at the amount of 50-70% by weight relative to the silkworm excrements, stirred at high speed and centrifuged to produce a first chlorophyll extract.
  • the extraction solvent is volatilized.
  • 6 kg of the chlorophyll extract obtained from the first and second extraction processes is added with 1-3% by weight of corn oil, 10-15% by weight of polyoxyethylene sorbitol fatty acid ether and 2-5% by weight of polyoxyethylene glycerin fatty acid ester, and emulsified by high-speed stirring .
  • 6 kg of the emulsified chlorophyll concentrate is mixed with 600 liters of colloidal silver-ion water, and stirred at high speed to produce a liquid chlorophyll composition containing chlorophyll.
  • Chlorophyll concentrate is extracted from the excrements of fifth-instar silkworms using extraction solvent. Namely, in a first extraction process, an extraction solvent with 95% alcohol concentration is added to 160 kg of silkworm excrements at the amount of 50-70% by weight relative to the silkworm excrements, and the solution is stirred at high speed and centrifuged to produce a first chlorophyll extract. In a second extraction process, to a residue remaining after the first extraction process, methylene chloride (dichloromethane) is added at the amount of 40-70% by weight, and the mixture is stirred at high speed and centrifuged to produce a second chlorophyll extract. In the centrifugation process, the extraction solvent is volatilized.
  • methylene chloride dichloromethane
  • chlorophyll-b 12.4 mg/lOOg
  • chlorophyll-a 30.9 mg/lOOg
  • linoleic acid 22 mg/lOOg
  • tocopherol (vitamin E) 1.6 mg/lOOg
  • the concentrate had a viable bacterial cell count of 5.0 x 10 and was positive to E . coli .
  • the inventive method for extracting chlorophyll from silkworm excrements and the composition produced thereby various problems in the prior process of extracting the chlorophyll using ultrasonic waves can be completely solved. Namely, problems, such as an increase in production cost caused by high equipment cost, the breakdown of the active ingredients of chlorophyll, and a reduction in productivity, can be solved. Also, according to the present invention, the colloidal silver-ion water is used to produce the chlorophyll composition so that the chlorophyll composition can be distributed without a need for freeze-storage .
  • the present invention has many effects in that it reduces production cost and increases productivity, and bacteria are not produced even when the composition is distributed at room temperature.

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  • Engineering & Computer Science (AREA)
  • Mining & Mineral Resources (AREA)
  • Paleontology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Civil Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Structural Engineering (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)

Abstract

La présente invention concerne un procédé d'extraction de chlorophylle à partir d'excréments de ver à soie, lequel comprend les étapes consistant: à ajouter un premier solvant d'extraction aux excréments de ver à soie au cinquième stade larvaire, à agiter le mélange et à centrifuger le mélange agité; à ajouter du chlorure de méthylène à un résidu, à ajouter le mélange contenant le résidu et à centrifuger le mélange contenant le résidu agité, le chlorure de méthylène étant volatilisé dans le processus de centrifugation, à concentrer l'extrait de chlorophylle obtenu des premier et second processus d'extraction, sous vide, afin de produire un concentré de chlorophylle; à ajouter de l'huile de maïs, du polyoxyéthylène sorbitol éther d'acides gras et du polyoxyéthylène glycérine ester d'acides gras au concentré de chlorophylle et à ajouter le mélange contenant le concentré de chlorophylle pour émulsifier le mélange et à mélanger le concentré de chlorophylle émulsifié ainsi que l'eau colloïdale à ions argent, et à agiter le mélange contenant des ions argent pour produire une composition de chlorophylle liquide contenant de la chlorophylle.
PCT/KR2004/000348 2003-02-20 2004-02-19 Procede d'extraction de chlorophylle a partir d'excrements de ver a soie et composition ainsi produite Ceased WO2004073828A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020030015887A KR20030032983A (ko) 2003-02-20 2003-02-20 엽록소 추출을 위한 조성물 및 그의 제조방법
KR10-2003-0015887 2003-02-20

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WO2004073828A1 true WO2004073828A1 (fr) 2004-09-02

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WO (1) WO2004073828A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110090469A (zh) * 2019-04-26 2019-08-06 青岛农业大学 一种从甘草中提取纯化甘草素和甘草次酸的方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100390176C (zh) * 2004-10-12 2008-05-28 大连理工大学 一种微波预处理提取蚕沙中叶绿素并制备叶绿酸铜钠盐的方法
KR100979224B1 (ko) * 2007-09-28 2010-09-01 광주과학기술원 안정화된 클로로필 ａ 조성물

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5933231A (ja) * 1982-08-19 1984-02-23 Seikou Kin 葉緑素抽出装置
KR100246934B1 (ko) * 1998-01-08 2000-03-15 김성호 잠분의 유효성분 추출장치
WO2002062161A1 (fr) * 2001-02-07 2002-08-15 Susumu Takayama Procede et appareil permettant de produire une boisson saine contenant de la chlorophylle
WO2003015539A1 (fr) * 2001-08-13 2003-02-27 Susumu Takayama Procede de production d'une boisson contenant de la chlorophylle
KR20040013430A (ko) * 2002-08-06 2004-02-14 스스무 다카야마 엽록소 음료 및 그 제조 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5933231A (ja) * 1982-08-19 1984-02-23 Seikou Kin 葉緑素抽出装置
KR100246934B1 (ko) * 1998-01-08 2000-03-15 김성호 잠분의 유효성분 추출장치
WO2002062161A1 (fr) * 2001-02-07 2002-08-15 Susumu Takayama Procede et appareil permettant de produire une boisson saine contenant de la chlorophylle
WO2003015539A1 (fr) * 2001-08-13 2003-02-27 Susumu Takayama Procede de production d'une boisson contenant de la chlorophylle
KR20040013430A (ko) * 2002-08-06 2004-02-14 스스무 다카야마 엽록소 음료 및 그 제조 방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110090469A (zh) * 2019-04-26 2019-08-06 青岛农业大学 一种从甘草中提取纯化甘草素和甘草次酸的方法

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