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WO2004067726A3 - Reactions isothermes pour amplification d'oligonucleotides - Google Patents

Reactions isothermes pour amplification d'oligonucleotides Download PDF

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Publication number
WO2004067726A3
WO2004067726A3 PCT/US2004/002718 US2004002718W WO2004067726A3 WO 2004067726 A3 WO2004067726 A3 WO 2004067726A3 US 2004002718 W US2004002718 W US 2004002718W WO 2004067726 A3 WO2004067726 A3 WO 2004067726A3
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WIPO (PCT)
Prior art keywords
reactions
reaction
amplification
exponential
chain reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/002718
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English (en)
Other versions
WO2004067726A2 (fr
Inventor
Ness Jeffrey Van
David J Galas
Ness Lori K Van
Jeffrey B Graybill
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Keck Graduate Institute of Applied Life Sciences
Ionian Technologies LLC
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Keck Graduate Institute of Applied Life Sciences
Ionian Technologies LLC
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Publication date
Application filed by Keck Graduate Institute of Applied Life Sciences, Ionian Technologies LLC filed Critical Keck Graduate Institute of Applied Life Sciences
Publication of WO2004067726A2 publication Critical patent/WO2004067726A2/fr
Anticipated expiration legal-status Critical
Publication of WO2004067726A3 publication Critical patent/WO2004067726A3/fr
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne une nouvelle catégorie de réactions isothermes pour amplifier l'ADN. Ces réactions homogènes synthétisent rapidement des oligonucléotides courts (par ex. 8-16 bases), spécifiés par la séquence d'une matrice d'amplification. Des versions des réactions peuvent se poursuivre soit en mode d'amplification linéaire, soit en mode d'amplification exponentielle. Ces deux formes de réaction font appel à des conditions simples et stables. Le taux d'amplification dépend entièrement des paramètres moléculaires régissant les interactions des molécules dans la réaction. La version exponentielle du procédé est une réaction moléculaire en chaîne utilisant les produits oligonucléotides de chaque réaction linéaire, afin de créer un producteur de davantage du même oligonucléotide. Il s'agit d'une réaction en chaîne à haut niveau de sensibilité qui peut être déclenchée par des séquences d'ADN spécifiques et peuvent effectuer des amplifications supérieures à 106 plis. Plusieurs réactions similaires de ladite catégorie sont décrites dans la présente invention. La robustesse, la vitesse et la sensibilité de la réaction exponentielle la rendent utile dans la détection rapide de la présence de petites quantités d'une séquence d'ADN spécifique dans un échantillon, et une série d'autres applications comprenant plusieurs utilisations courantes de la réaction en chaîne de la polymérase (PCR).
PCT/US2004/002718 2003-01-29 2004-01-29 Reactions isothermes pour amplification d'oligonucleotides Ceased WO2004067726A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44365203P 2003-01-29 2003-01-29
US60/443,652 2003-01-29

Publications (2)

Publication Number Publication Date
WO2004067726A2 WO2004067726A2 (fr) 2004-08-12
WO2004067726A3 true WO2004067726A3 (fr) 2005-12-15

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ID=32825356

Family Applications (1)

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PCT/US2004/002718 Ceased WO2004067726A2 (fr) 2003-01-29 2004-01-29 Reactions isothermes pour amplification d'oligonucleotides

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WO (1) WO2004067726A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562264B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US11845991B2 (en) 2009-02-03 2023-12-19 Exact Sciences Corporation Fecal sample processing and analysis comprising detection of blood
US12110550B2 (en) 2020-07-13 2024-10-08 Singular Genomics Systems, Inc. Methods of amplifying circular polynucleotides in situ

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
CA2654266A1 (fr) 2006-06-12 2007-12-27 Oncomethylome Sciences S.A. Marqueurs de methylation destines a une detection et un pronostic precoces de cancers du colon
CN101849019A (zh) 2007-07-09 2010-09-29 微敏医疗技术有限公司 基于其nad依赖性dna连接酶活性来检测微生物
US9322065B2 (en) 2007-09-17 2016-04-26 Mdxhealth Sa Methods for determining methylation of the TWIST1 gene for bladder cancer detection
CN101932723B (zh) 2007-09-17 2014-09-10 肿瘤甲基化科学公司 改进的对mage-a表达的检测
EP2677041A3 (fr) 2008-02-19 2014-04-09 MDxHealth SA Détection et pronostic du cancer du poumon
CA2718092C (fr) 2008-03-21 2019-03-19 Oncomethylome Sciences S.A. Detection et pronostic du cancer du col de l'uterus
CA2679750A1 (fr) 2008-09-23 2010-03-23 Nexus Dx, Inc. Methodes de detection d'acides nucleiques dans un echantillon
US20120028253A1 (en) * 2009-01-05 2012-02-02 Xiaolong Wang Method for amplifying oligonucleotide and small rna by using polymerase-endonuclease chain reaction
WO2010102823A1 (fr) 2009-03-13 2010-09-16 Oncomethylome Sciences Sa Nouveaux marqueurs pour la détection du cancer de la vessie
AU2010225125A1 (en) 2009-03-17 2011-10-20 Glaxosmithkline Biologicals S.A. Improved detection of gene expression
WO2011036173A1 (fr) 2009-09-24 2011-03-31 Oncomethylome Sciences S.A. Détection et pronostic du cancer du col de l'utérus
WO2011135058A2 (fr) 2010-04-30 2011-11-03 Mdxhealth Sa Procédé de détection de modifications épigénétiques
EP2420579A1 (fr) 2010-08-17 2012-02-22 QIAGEN GmbH Amplification isotherme dépendante de l'hélicase à l'aide d'enzymes d'ébréchage
US20120058481A1 (en) 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
US9845495B2 (en) 2010-12-10 2017-12-19 Abbott Laboratories Method and kit for detecting target nucleic acid
GB201206209D0 (en) 2012-04-06 2012-05-23 Univ Leuven Kath Marker gene based diagnosis, staging and prognosis of prostate caner
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
WO2013155056A1 (fr) 2012-04-09 2013-10-17 Envirologix Inc. Compositions et procédés permettant de quantifier une séquence d'acides nucléiques dans un échantillon
GB201322034D0 (en) 2013-12-12 2014-01-29 Almac Diagnostics Ltd Prostate cancer classification
WO2015114469A2 (fr) 2014-01-15 2015-08-06 Abbott Japan Co., Ltd Adn pour conversion de séquences et procédés de détection
JP6773651B2 (ja) 2014-10-14 2020-10-21 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ ポリｄｎａスペーサー配列を有する配列変換およびシグナル増幅ｄｎａならびにそれらを用いた検出方法
US10604790B2 (en) 2014-12-24 2020-03-31 Abbott Laboratories Sequence conversion and signal amplifier DNA cascade reactions and detection methods using same
CN107532124B (zh) 2015-03-27 2022-08-09 精密科学公司 检测食管疾病
GB201510684D0 (en) 2015-06-17 2015-08-05 Almac Diagnostics Ltd Gene signatures predictive of metastatic disease
WO2017141068A1 (fr) 2016-02-16 2017-08-24 The University Of Tokyo Composant informatique moléculaire et procédé d'informatique moléculaire
WO2017141067A1 (fr) 2016-02-16 2017-08-24 The University Of Tokyo Procédé d'élimination de l'amplification de fond d'acides nucléiques cibles
GB201605110D0 (en) 2016-03-24 2016-05-11 Mologic Ltd Detecting sepsis
US20200048691A1 (en) * 2017-04-17 2020-02-13 Montana State University Switch-like isothermal dna amplification demonstrating a non-linear amplification rate
GB201805479D0 (en) 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201902458D0 (en) 2019-02-22 2019-04-10 Mologic Ltd Treatment stratification for an excerbation of inflamation
CN110408678A (zh) * 2019-07-26 2019-11-05 宁儿医院股份有限公司 单管型多重微生物检测体系及其即时检测方法
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230086471A1 (en) * 2020-02-15 2023-03-23 Agency For Science, Technology And Research A method to amplify a nucleic acid
GB202019059D0 (en) * 2020-12-03 2021-01-20 Univ Birmingham Target rna detection
JPWO2021192965A1 (fr) * 2020-03-26 2021-09-30
EP4150126A4 (fr) * 2020-05-12 2024-06-19 Singular Genomics Systems, Inc. Procédés d'amplification d'acides nucléiques
CN112760362B (zh) * 2021-02-04 2023-07-07 中国人民解放军陆军特色医学中心 一种用于寡核苷酸扩增的环形信号扩增模板及其应用
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6130038A (en) * 1996-07-16 2000-10-10 Gen-Probe Incorporated Method for amplifying target nucleic acids using modified primers
WO2001094544A2 (fr) * 2000-06-02 2001-12-13 New England Biolabs, Inc. Clonage et production de l'endonuclease de coupure n.$m(f)i$m(g)bst$m(f)/i$m(g)nbi et procedes associes d'utilisation des endonucleases de coupure dans l'amplification par deplacement de simple brin

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US6130038A (en) * 1996-07-16 2000-10-10 Gen-Probe Incorporated Method for amplifying target nucleic acids using modified primers
WO2001094544A2 (fr) * 2000-06-02 2001-12-13 New England Biolabs, Inc. Clonage et production de l'endonuclease de coupure n.$m(f)i$m(g)bst$m(f)/i$m(g)nbi et procedes associes d'utilisation des endonucleases de coupure dans l'amplification par deplacement de simple brin

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MORGAN R.D. ET AL: "Characterization of the specific DNA nicking activity of Restriction endonuclease N.BstNBI.", BIOL CHEM., vol. 381, no. 11, November 2000 (2000-11-01), pages 1123 - 1125, XP001009735 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9562264B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US9562263B2 (en) 2007-07-14 2017-02-07 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US11845991B2 (en) 2009-02-03 2023-12-19 Exact Sciences Corporation Fecal sample processing and analysis comprising detection of blood
US12110550B2 (en) 2020-07-13 2024-10-08 Singular Genomics Systems, Inc. Methods of amplifying circular polynucleotides in situ
US12139759B2 (en) 2020-07-13 2024-11-12 Singular Genomics Systems, Inc. Methods of sequencing circular template polynucleotides

Also Published As

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