IS6206A - Aðferð til hlutfallslegrar skömmtunar á metýleringu á bösum af sýtósín-gerð í DNA sýnum - Google Patents
Aðferð til hlutfallslegrar skömmtunar á metýleringu á bösum af sýtósín-gerð í DNA sýnumInfo
- Publication number
- IS6206A IS6206A IS6206A IS6206A IS6206A IS 6206 A IS6206 A IS 6206A IS 6206 A IS6206 A IS 6206A IS 6206 A IS6206 A IS 6206A IS 6206 A IS6206 A IS 6206A
- Authority
- IS
- Iceland
- Prior art keywords
- methylation
- bases
- dna samples
- cytosine
- dna
- Prior art date
Links
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 title abstract 6
- 229940104302 cytosine Drugs 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 3
- 230000011987 methylation Effects 0.000 title abstract 2
- 238000007069 methylation reaction Methods 0.000 title abstract 2
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 abstract 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical class C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/10—Detection mode being characterised by the assay principle
- C12Q2565/137—Chromatographic separation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19935772A DE19935772C2 (de) | 1999-07-26 | 1999-07-26 | Verfahren zur relativen Quantifizierung der Methylierung von Cytosin Basen in DNA-Proben |
| PCT/DE2000/002490 WO2001007647A2 (de) | 1999-07-26 | 2000-07-25 | Verfahren zur relativen quantifizierung der methylierung von cytosin basen in dna-proben |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IS6206A true IS6206A (is) | 2001-12-18 |
Family
ID=7916564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IS6206A IS6206A (is) | 1999-07-26 | 2001-12-18 | Aðferð til hlutfallslegrar skömmtunar á metýleringu á bösum af sýtósín-gerð í DNA sýnum |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7153671B2 (is) |
| EP (1) | EP1204765B1 (is) |
| JP (1) | JP3637308B2 (is) |
| AT (1) | ATE305520T1 (is) |
| AU (1) | AU772002B2 (is) |
| CA (1) | CA2378569A1 (is) |
| DE (2) | DE19935772C2 (is) |
| IL (1) | IL147845A0 (is) |
| IS (1) | IS6206A (is) |
| WO (1) | WO2001007647A2 (is) |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19951189C2 (de) * | 1999-10-15 | 2003-11-06 | Epigenomics Ag | Verfahren zur Unterscheidung von 5-Position-Methylierungsänderungen von Cytosin-Basen und Cytosin-zu-Thymin-Mutationen und zum Nachweis von single nucleotide polymorphisms (SNPs) oder Punktmutation in genomischer DNA |
| DE10056802B4 (de) * | 2000-11-14 | 2005-06-16 | Epigenomics Ag | Verfahren zur Detektion von Methylierungszuständen zur toxikologischen Diagnostik |
| DE10061348C2 (de) * | 2000-12-06 | 2002-10-24 | Epigenomics Ag | Verfahren zur Quantifizierung von Cytosin-Methylierungen in komplex amplifizierter genomischer DNA |
| DE10065814B4 (de) | 2000-12-22 | 2004-07-08 | Epigenomics Ag | Verfahren für die simultane Amplifikation vieler Sequenzen in einer PCR-Reaktion und deren Markierung |
| DE10119468A1 (de) * | 2001-04-12 | 2002-10-24 | Epigenomics Ag | Mikroarray-Verfahren zur Anreicherung von DNA-Fragmenten aus komplexen Mischungen |
| DE10128508A1 (de) * | 2001-06-14 | 2003-02-06 | Epigenomics Ag | Verfahren und Nukleinsäuren für die Differenzierung von Prostata-Tumoren |
| DE10132211A1 (de) * | 2001-06-27 | 2003-01-16 | Epigenomics Ag | Nachweis spezifischer Dinukleotide in DNA-Proben durch Fluoreszenzresonanzenergietransfer (FRET) |
| DE10151069A1 (de) * | 2001-10-05 | 2003-04-30 | Epigenomics Ag | Verfahren zum Nachweis von DNA-Methylierung mittels markierten S-Adenosylmethioninanaloga |
| US20110151438A9 (en) | 2001-11-19 | 2011-06-23 | Affymetrix, Inc. | Methods of Analysis of Methylation |
| WO2003044226A2 (en) * | 2001-11-23 | 2003-05-30 | Epigenomics Ag | Method and nucleic acids for the analysis of a lymphoid cell proliferative disorder |
| DE10160983B4 (de) * | 2001-12-05 | 2004-12-09 | Epigenomics Ag | Verfahren und Integrierte Vorrichtung zum Nachweis von Cytosinmethylierungen |
| EP1340818A1 (en) * | 2002-02-27 | 2003-09-03 | Epigenomics AG | Method and nucleic acids for the analysis of a colon cell proliferative disorder |
| AU2003900368A0 (en) * | 2003-01-24 | 2003-02-13 | Human Genetic Signatures Pty Ltd | Assay for nucleic acid molecules |
| WO2007039101A1 (en) * | 2005-09-30 | 2007-04-12 | Epigenomics Ag | Method for the quantification of nucleic acids, in particular bisulfite treated dna |
| US7932027B2 (en) * | 2005-02-16 | 2011-04-26 | Epigenomics Ag | Method for determining the methylation pattern of a polynucleic acid |
| US20060292585A1 (en) * | 2005-06-24 | 2006-12-28 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| US20070122818A1 (en) * | 2005-10-31 | 2007-05-31 | Abhijit Mazumder | Gene methylation assay controls |
| US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
| US7901882B2 (en) | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| JP2008136404A (ja) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法 |
| US7899626B2 (en) * | 2007-01-10 | 2011-03-01 | Illumina, Inc. | System and method of measuring methylation of nucleic acids |
| US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| GB0714058D0 (en) * | 2007-07-19 | 2007-08-29 | Abertec Ltd | detection of methylation in nucleic acid sequences |
| US20110165565A1 (en) | 2008-01-03 | 2011-07-07 | The Johns Hopkins University | Compositions and methods for polynucleotide extraction and methylation detection |
| US20110177512A1 (en) * | 2010-01-19 | 2011-07-21 | Predictive Biosciences, Inc. | Method for assuring amplification of an abnormal nucleic acid in a sample |
| US10793895B2 (en) * | 2015-08-24 | 2020-10-06 | Seven Bridges Genomics Inc. | Systems and methods for epigenetic analysis |
| US10584380B2 (en) | 2015-09-01 | 2020-03-10 | Seven Bridges Genomics Inc. | Systems and methods for mitochondrial analysis |
| US10724110B2 (en) | 2015-09-01 | 2020-07-28 | Seven Bridges Genomics Inc. | Systems and methods for analyzing viral nucleic acids |
| CN113930487B (zh) * | 2020-06-29 | 2023-03-17 | 广州市基准医疗有限责任公司 | 一种新型多样本多片段dna甲基化检测方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4212939A1 (de) * | 1992-04-18 | 1993-10-21 | Schottmann Bernd Dr Med | Verfahren zum analytischen Nachweis von Mutationen in informationstragenden Biopolymeren |
| US5837832A (en) * | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| AU7829398A (en) * | 1997-06-09 | 1998-12-30 | University Of Southern California | A cancer diagnostic method based upon dna methylation differences |
| DE19754482A1 (de) * | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US6046002A (en) * | 1998-01-05 | 2000-04-04 | The Board Of Trustees Of The Leland Stanford Junior University | Highly parallel and sensitive method for identifying drugs and drug targets |
| US6379889B1 (en) * | 1999-11-04 | 2002-04-30 | Agilent Technologies, Inc. | Multiplexing methods for identifying nucleic acids using denaturing liquid chromatography |
-
1999
- 1999-07-26 DE DE19935772A patent/DE19935772C2/de not_active Expired - Fee Related
-
2000
- 2000-07-25 AU AU69818/00A patent/AU772002B2/en not_active Ceased
- 2000-07-25 DE DE50011259T patent/DE50011259D1/de not_active Expired - Lifetime
- 2000-07-25 IL IL14784500A patent/IL147845A0/xx unknown
- 2000-07-25 AT AT00958186T patent/ATE305520T1/de not_active IP Right Cessation
- 2000-07-25 WO PCT/DE2000/002490 patent/WO2001007647A2/de not_active Ceased
- 2000-07-25 EP EP00958186A patent/EP1204765B1/de not_active Expired - Lifetime
- 2000-07-25 CA CA002378569A patent/CA2378569A1/en not_active Abandoned
- 2000-07-25 JP JP2001512913A patent/JP3637308B2/ja not_active Expired - Fee Related
-
2001
- 2001-12-18 IS IS6206A patent/IS6206A/is unknown
-
2002
- 2002-01-25 US US10/057,776 patent/US7153671B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU772002B2 (en) | 2004-04-08 |
| JP3637308B2 (ja) | 2005-04-13 |
| EP1204765A2 (de) | 2002-05-15 |
| AU6981800A (en) | 2001-02-13 |
| ATE305520T1 (de) | 2005-10-15 |
| DE19935772C2 (de) | 2002-11-07 |
| CA2378569A1 (en) | 2001-02-01 |
| DE19935772A1 (de) | 2001-02-08 |
| WO2001007647A9 (de) | 2003-01-23 |
| DE50011259D1 (de) | 2005-11-03 |
| EP1204765B1 (de) | 2005-09-28 |
| US7153671B2 (en) | 2006-12-26 |
| JP2003516117A (ja) | 2003-05-13 |
| US20030032026A1 (en) | 2003-02-13 |
| IL147845A0 (en) | 2002-08-14 |
| WO2001007647A3 (de) | 2001-08-09 |
| WO2001007647A2 (de) | 2001-02-01 |
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