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WO2004065543A3 - Dispositifs et procedes pour recuperer une molecule cible d'un echantillon - Google Patents

Dispositifs et procedes pour recuperer une molecule cible d'un echantillon Download PDF

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Publication number
WO2004065543A3
WO2004065543A3 PCT/US2004/001041 US2004001041W WO2004065543A3 WO 2004065543 A3 WO2004065543 A3 WO 2004065543A3 US 2004001041 W US2004001041 W US 2004001041W WO 2004065543 A3 WO2004065543 A3 WO 2004065543A3
Authority
WO
WIPO (PCT)
Prior art keywords
unit
sample
target molecule
region
recovery
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/001041
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English (en)
Other versions
WO2004065543A2 (fr
Inventor
Duncan H Whitney
Walker M Sloan Iii
Stephen G Kieffer-Higgins
Anthony P Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exact Sciences Corp
Original Assignee
Exact Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exact Sciences Corp filed Critical Exact Sciences Corp
Publication of WO2004065543A2 publication Critical patent/WO2004065543A2/fr
Publication of WO2004065543A3 publication Critical patent/WO2004065543A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif permettant de récupérer une molécule cible contenue dans un échantillon biologique. Ce dispositif présente une première unité définissant une chambre qui comporte une première zone pour la réception d'un échantillon et une seconde zone contenant au moins un milieu électrophorétique. Ce dispositif présente une seconde unité s'étendant d'une première extrémité à une seconde extrémité et cette seconde unité peut être interfacée avec la première unité. Une structure maillée est située au niveau de la seconde extrémité de la seconde unité et imprégnée d'un milieu électrophorétique contenant des sondes de capture copolymérisées. La seconde zone est située entre la première zone et la structure maillée lorsque la première unité est interfacée avec la seconde unité.
PCT/US2004/001041 2003-01-16 2004-01-15 Dispositifs et procedes pour recuperer une molecule cible d'un echantillon Ceased WO2004065543A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44050003P 2003-01-16 2003-01-16
US60/440,500 2003-01-16

Publications (2)

Publication Number Publication Date
WO2004065543A2 WO2004065543A2 (fr) 2004-08-05
WO2004065543A3 true WO2004065543A3 (fr) 2004-12-23

Family

ID=32771822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/001041 Ceased WO2004065543A2 (fr) 2003-01-16 2004-01-15 Dispositifs et procedes pour recuperer une molecule cible d'un echantillon

Country Status (1)

Country Link
WO (1) WO2004065543A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007512509A (ja) 2003-11-05 2007-05-17 エグザクト サイエンシーズ コーポレイション 反復的親和性分離およびその使用
CN103884570B (zh) * 2014-03-28 2016-06-15 北京纳晶生物科技有限公司 一体化电洗脱收集装置

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169511A (en) * 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5833927A (en) * 1992-09-18 1998-11-10 Amersham International Plc Device and method for affinity separation
WO1998051823A1 (fr) * 1997-05-16 1998-11-19 Mosaic Technologies Analyse electrophoretique de molecules au moyen de sondes immobilisees
WO2001007915A2 (fr) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Methode et dispositif d'analyse de specimens biologiques
US20020119480A1 (en) * 1999-02-26 2002-08-29 Lawrence Weir Purification devices comprising immobilized capture probes and uses therefor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169511A (en) * 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5833927A (en) * 1992-09-18 1998-11-10 Amersham International Plc Device and method for affinity separation
WO1998051823A1 (fr) * 1997-05-16 1998-11-19 Mosaic Technologies Analyse electrophoretique de molecules au moyen de sondes immobilisees
US20020119480A1 (en) * 1999-02-26 2002-08-29 Lawrence Weir Purification devices comprising immobilized capture probes and uses therefor
WO2001007915A2 (fr) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Methode et dispositif d'analyse de specimens biologiques

Also Published As

Publication number Publication date
WO2004065543A2 (fr) 2004-08-05

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