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WO2004065543A3 - Devices and method for recovery of a target molecule from a sample - Google Patents

Devices and method for recovery of a target molecule from a sample Download PDF

Info

Publication number
WO2004065543A3
WO2004065543A3 PCT/US2004/001041 US2004001041W WO2004065543A3 WO 2004065543 A3 WO2004065543 A3 WO 2004065543A3 US 2004001041 W US2004001041 W US 2004001041W WO 2004065543 A3 WO2004065543 A3 WO 2004065543A3
Authority
WO
WIPO (PCT)
Prior art keywords
unit
sample
target molecule
region
recovery
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2004/001041
Other languages
French (fr)
Other versions
WO2004065543A2 (en
Inventor
Duncan H Whitney
Walker M Sloan Iii
Stephen G Kieffer-Higgins
Anthony P Shuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exact Sciences Corp
Original Assignee
Exact Sciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exact Sciences Corp filed Critical Exact Sciences Corp
Publication of WO2004065543A2 publication Critical patent/WO2004065543A2/en
Publication of WO2004065543A3 publication Critical patent/WO2004065543A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a device for recovering a target molecule from a biological sample. The device includes a first unit defining a chamber, and the chamber includes a first region for receiving a sample and a second region including at least one electrophoretic medium. The device includes a second unit extending from a first end to a second end, and the second unit is interfaceable with the first unit. A mesh structure is located at the second end of the second unit and is impregnated with an electrophoretic medium that includes copolymerized capture probes. The second region is located between the first region and the mesh structure when the first unit is interfaced with the second unit.
PCT/US2004/001041 2003-01-16 2004-01-15 Devices and method for recovery of a target molecule from a sample Ceased WO2004065543A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44050003P 2003-01-16 2003-01-16
US60/440,500 2003-01-16

Publications (2)

Publication Number Publication Date
WO2004065543A2 WO2004065543A2 (en) 2004-08-05
WO2004065543A3 true WO2004065543A3 (en) 2004-12-23

Family

ID=32771822

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/001041 Ceased WO2004065543A2 (en) 2003-01-16 2004-01-15 Devices and method for recovery of a target molecule from a sample

Country Status (1)

Country Link
WO (1) WO2004065543A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1685395A2 (en) 2003-11-05 2006-08-02 EXACT Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor
CN103884570B (en) * 2014-03-28 2016-06-15 北京纳晶生物科技有限公司 Integration electroelution collection device

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169511A (en) * 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5833927A (en) * 1992-09-18 1998-11-10 Amersham International Plc Device and method for affinity separation
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
WO2001007915A2 (en) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Layered device with capture regions for cellular analysis
US20020119480A1 (en) * 1999-02-26 2002-08-29 Lawrence Weir Purification devices comprising immobilized capture probes and uses therefor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5169511A (en) * 1988-11-29 1992-12-08 Isco, Inc. Capillary electrophoresis technique
US5833927A (en) * 1992-09-18 1998-11-10 Amersham International Plc Device and method for affinity separation
WO1998051823A1 (en) * 1997-05-16 1998-11-19 Mosaic Technologies Electrophoretic analysis of molecules using immobilized probes
US20020119480A1 (en) * 1999-02-26 2002-08-29 Lawrence Weir Purification devices comprising immobilized capture probes and uses therefor
WO2001007915A2 (en) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Layered device with capture regions for cellular analysis

Also Published As

Publication number Publication date
WO2004065543A2 (en) 2004-08-05

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