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WO2004055502A3 - Procede de mesure d'une quantite de photons proportionnelle a la quantite de photons recus par l'objet et dispositif associe - Google Patents

Procede de mesure d'une quantite de photons proportionnelle a la quantite de photons recus par l'objet et dispositif associe Download PDF

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Publication number
WO2004055502A3
WO2004055502A3 PCT/FR2003/050160 FR0350160W WO2004055502A3 WO 2004055502 A3 WO2004055502 A3 WO 2004055502A3 FR 0350160 W FR0350160 W FR 0350160W WO 2004055502 A3 WO2004055502 A3 WO 2004055502A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
measuring
photons
photon
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/FR2003/050160
Other languages
English (en)
Other versions
WO2004055502A2 (fr
Inventor
Philippe Rizo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Apibio SAS
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Apibio SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Apibio SAS filed Critical Commissariat a lEnergie Atomique CEA
Publication of WO2004055502A2 publication Critical patent/WO2004055502A2/fr
Publication of WO2004055502A3 publication Critical patent/WO2004055502A3/fr
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Landscapes

  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un dispositif de mesure de fluorescence comportant une source émettrice de photons (1) destinée à émettre un faisceau de photons vers un échantillon à analyser, le dispositif comprenant également des moyens de détection (11) d'une lumière de fluorescence émise par l'échantillon en réponse auxphotons du faisceau reçus par l'échantillon. Le dispositif comporte en outre des moyens de prélèvement (3) aptes à prélever, au cours de la mesure de fluorescence, une fraction déterminée du faisceau de photons émis par la source de photons, des moyens de mesure (4) de ladite fraction du faisceau de photons prélevée, et des moyens d'intégration (5) permettant d'intégrer la fraction déterminée du faisceau de photons. L'invention concerne également un procédé capable de fournir
PCT/FR2003/050160 2002-12-12 2003-12-11 Procede de mesure d'une quantite de photons proportionnelle a la quantite de photons recus par l'objet et dispositif associe Ceased WO2004055502A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR02/15734 2002-12-12
FR0215734A FR2848669B1 (fr) 2002-12-12 2002-12-12 Procede de mesure d'une quantite de photons proportionnelle a la quantite de photons recus par l'objet et dispositif associe.

Publications (2)

Publication Number Publication Date
WO2004055502A2 WO2004055502A2 (fr) 2004-07-01
WO2004055502A3 true WO2004055502A3 (fr) 2004-08-12

Family

ID=32338729

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/050160 Ceased WO2004055502A2 (fr) 2002-12-12 2003-12-11 Procede de mesure d'une quantite de photons proportionnelle a la quantite de photons recus par l'objet et dispositif associe

Country Status (2)

Country Link
FR (1) FR2848669B1 (fr)
WO (1) WO2004055502A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8749792B2 (en) 2011-09-02 2014-06-10 Commissariat A L'energie Atomique Et Aux Energies Alternatives Device for optical measurement of materials, using multiplexing of light
US9046487B2 (en) 2011-09-02 2015-06-02 Commissariat à l'énergie atomique et aux énergies alternatives Device for lighting an object, with light source provided with a member for sampling a portion of said light, application to the measurement of flux variations of the source

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100427923C (zh) * 2006-03-08 2008-10-22 东华大学 多功能芯片检测装置
FR2980577B1 (fr) 2011-09-26 2013-09-20 Biomerieux Sa Systeme de detection et/ou de quantification in vitro par fluorimetrie
FR3035716B1 (fr) 2015-04-30 2019-06-21 Biomerieux Machine et procede pour la detection automatisee in vitro d'analytes mettant en oeuvre une decomposition spectrale chromatique d'une reponse optique
EP3973533A1 (fr) 2019-05-22 2022-03-30 Biomerieux Procédés et systèmes de fabrication d'un réacteur de dosage de production

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055768A (en) * 1976-09-07 1977-10-25 Bromberg Nathan S Light measuring apparatus
US5639668A (en) * 1995-09-14 1997-06-17 Boehringer Mannheim Corporation Optical apparatus for performing an immunoassay
US5925884A (en) * 1996-02-21 1999-07-20 Biomerieux, Inc. Fluorescence station for biological sample testing machine
US6024920A (en) * 1998-04-21 2000-02-15 Bio-Rad Laboratories, Inc. Microplate scanning read head
GB2360162A (en) * 2000-01-07 2001-09-12 Axon Instr Inc Scanning microscope with rotating objective
US20020071121A1 (en) * 1999-01-25 2002-06-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055768A (en) * 1976-09-07 1977-10-25 Bromberg Nathan S Light measuring apparatus
US5639668A (en) * 1995-09-14 1997-06-17 Boehringer Mannheim Corporation Optical apparatus for performing an immunoassay
US5925884A (en) * 1996-02-21 1999-07-20 Biomerieux, Inc. Fluorescence station for biological sample testing machine
US6024920A (en) * 1998-04-21 2000-02-15 Bio-Rad Laboratories, Inc. Microplate scanning read head
US20020071121A1 (en) * 1999-01-25 2002-06-13 Amnis Corporation Imaging and analyzing parameters of small moving objects such as cells
GB2360162A (en) * 2000-01-07 2001-09-12 Axon Instr Inc Scanning microscope with rotating objective

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8749792B2 (en) 2011-09-02 2014-06-10 Commissariat A L'energie Atomique Et Aux Energies Alternatives Device for optical measurement of materials, using multiplexing of light
US9046487B2 (en) 2011-09-02 2015-06-02 Commissariat à l'énergie atomique et aux énergies alternatives Device for lighting an object, with light source provided with a member for sampling a portion of said light, application to the measurement of flux variations of the source

Also Published As

Publication number Publication date
WO2004055502A2 (fr) 2004-07-01
FR2848669A1 (fr) 2004-06-18
FR2848669B1 (fr) 2005-09-02

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