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WO2003016569A1 - Nucleic acid amplification methods - Google Patents

Nucleic acid amplification methods Download PDF

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Publication number
WO2003016569A1
WO2003016569A1 PCT/JP2002/008357 JP0208357W WO03016569A1 WO 2003016569 A1 WO2003016569 A1 WO 2003016569A1 JP 0208357 W JP0208357 W JP 0208357W WO 03016569 A1 WO03016569 A1 WO 03016569A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid amplification
amplification methods
methods
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/JP2002/008357
Other languages
English (en)
French (fr)
Inventor
Tatsuji Enoki
Hiroaki Sagawa
Ikunoshin Kato
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio Inc
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Priority to KR10-2004-7002070A priority Critical patent/KR20040028991A/ko
Priority to US10/486,645 priority patent/US7056671B2/en
Priority to CA002458127A priority patent/CA2458127A1/en
Priority to EP02758853A priority patent/EP1420069A4/en
Priority to JP2003521876A priority patent/JPWO2003016569A1/ja
Priority to AU2002325538A priority patent/AU2002325538B2/en
Priority to EA200400329A priority patent/EA006066B1/ru
Publication of WO2003016569A1 publication Critical patent/WO2003016569A1/ja
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PCT/JP2002/008357 2001-08-20 2002-08-20 Nucleic acid amplification methods Ceased WO2003016569A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
KR10-2004-7002070A KR20040028991A (ko) 2001-08-20 2002-08-20 핵산 증폭 방법
US10/486,645 US7056671B2 (en) 2001-08-20 2002-08-20 Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
CA002458127A CA2458127A1 (en) 2001-08-20 2002-08-20 Nucleic acid amplification methods
EP02758853A EP1420069A4 (en) 2001-08-20 2002-08-20 nucleic acid amplification
JP2003521876A JPWO2003016569A1 (ja) 2001-08-20 2002-08-20 核酸の増幅方法
AU2002325538A AU2002325538B2 (en) 2001-08-20 2002-08-20 Nucleic acid amplification methods
EA200400329A EA006066B1 (ru) 2001-08-20 2002-08-20 Способы амплификации нуклеиновых кислот

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001249497 2001-08-20
JP2001-249497 2001-08-20

Publications (1)

Publication Number Publication Date
WO2003016569A1 true WO2003016569A1 (en) 2003-02-27

Family

ID=19078491

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2002/008357 Ceased WO2003016569A1 (en) 2001-08-20 2002-08-20 Nucleic acid amplification methods

Country Status (10)

Country Link
US (1) US7056671B2 (ja)
EP (1) EP1420069A4 (ja)
JP (1) JPWO2003016569A1 (ja)
KR (1) KR20040028991A (ja)
CN (1) CN1633505A (ja)
AU (1) AU2002325538B2 (ja)
CA (1) CA2458127A1 (ja)
EA (1) EA006066B1 (ja)
TW (1) TWI257426B (ja)
WO (1) WO2003016569A1 (ja)

Cited By (9)

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JP2010094091A (ja) * 2008-10-17 2010-04-30 National Agriculture & Food Research Organization Ｄｎａ増幅法
JP2010539956A (ja) * 2007-10-04 2010-12-24 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 核酸増幅
WO2011055737A1 (ja) * 2009-11-06 2011-05-12 株式会社ニッポンジーン 耐熱性鎖置換型dnaポリメラーゼ及び該dnaポリメラーゼの生産方法
US9631231B2 (en) 2012-04-09 2017-04-25 Envirologix Inc. Compositions and methods for amplifying a nucleic acid sequence comprising a primer oligonucleotide with a 3′-terminal region comprising a 2′-modified nucleotide
US10364458B2 (en) 2014-07-16 2019-07-30 Tangen Biosciences, Inc. Isothermal methods for amplifying nucleic acid samples
US11505836B2 (en) 2014-04-22 2022-11-22 Envirologix Inc. Compositions and methods for enhancing and/or predicting DNA amplification
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
US12043866B2 (en) 2010-08-13 2024-07-23 Envirologix Inc. Compositions and methods for quantifying a nucleic acid sequence in a sample
US12392001B2 (en) 2014-10-20 2025-08-19 Envirologix Inc. Compositions and methods for detecting an RNA virus

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US7846733B2 (en) * 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
BR0205268A (pt) * 2001-03-09 2004-11-30 Nugen Technologies Inc Processos e composições para a mplificação de sequências de rna
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US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
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US7288373B2 (en) * 2003-05-02 2007-10-30 Human Genetic Signatures Pty Ltd. Treatment of methylated nucleic acid
US7799525B2 (en) * 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
DE602004018801D1 (de) * 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nukleinsäurenachweistest
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
ATE476439T1 (de) * 2004-09-10 2010-08-15 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
US7833942B2 (en) * 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
AU2005318874B2 (en) * 2004-12-23 2007-05-03 Human Genetic Signatures Pty Ltd Detection of human papilloma virus
CN101203618B (zh) * 2005-05-26 2013-03-13 人类遗传标记控股有限公司 使用含有非常规碱基的引物的等温链置换扩增
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JP2010505438A (ja) * 2006-10-09 2010-02-25 オキシテック・リミテッド 核酸配列を増幅および検出する方法
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JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
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Cited By (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010539956A (ja) * 2007-10-04 2010-12-24 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 核酸増幅
JP2010094091A (ja) * 2008-10-17 2010-04-30 National Agriculture & Food Research Organization Ｄｎａ増幅法
WO2011055737A1 (ja) * 2009-11-06 2011-05-12 株式会社ニッポンジーン 耐熱性鎖置換型dnaポリメラーゼ及び該dnaポリメラーゼの生産方法
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AU2002325538B2 (en) 2007-03-22
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EA006066B1 (ru) 2005-08-25
CA2458127A1 (en) 2003-02-27
TWI257426B (en) 2006-07-01
EP1420069A1 (en) 2004-05-19
CN1633505A (zh) 2005-06-29
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US7056671B2 (en) 2006-06-06
US20050059003A1 (en) 2005-03-17

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